The presence of iron in circulating monocytes is well known as they play an essential role in iron recycling. It has been demonstrated that the iron content of blood cells can be measured through their magnetic behavior; however, the magnetic properties of different monocyte subtypes remain unknown. In this study, we report for the first time, the magnetic behavior of classical, intermediate and non-classical monocytes, which is related to their iron storage capacity. The magnetic properties of monocytes were compared to other blood cells, such as lymphocytes and red blood cells in the oxyhemoglobin and methemoglobin states, and a cancer cell type. For this analysis, we used an instrument referred to as Cell Tracking Velocimetry (CTV), which quantitatively characterizes the magnetic behavior of biological entities. Our results demonstrate that significant fractions of the intermediate and non-classical monocytes have high magnetophoretic mobilities, equivalent to methemoglobin red blood cells and higher than the classical subset, suggesting their higher iron storage capacities. Moreover, our findings have implications for the immunomagnetic separation industry; we demonstrate that negative magnetic isolation techniques for recovering monocytes from blood should be used with caution, as it is possible to lose magnetic monocytes when using this technique.
Plasma treatment increases the surface energy of condensed phases: solids and liquids.Two independent methods of the quantification of the influence imposed by a cold radiofrequency air plasma treatment on the surface properties of silicone oils (polydimethylsiloxane) of various molecular masses and castor oil are introduced.Under the first method the water droplet coated by oils was exposed to the cold air radiofrequency plasma, resulting in an increase of oil/air surface energy.An expression relating the oil/air surface energy to the apparent contact angle of the water droplet coated with oil was derived.The apparent contact angle was established experimentally.Calculation of the oil/air surface energy and spreading parameter was carried out for the various plasmatreated silicone and castor oils.The second method is based on the measurement of the electret response of the plasma-treated liquids.
The paper examines the electret response of cold plasma treated polypropylene and low‐density polyethylene films. Films were loaded with oscillating mechanical load; the output voltage was registered by a lock‐in amplifier. In parallel, hydrophobic recovery of the films was studied by the measurement of the apparent water contact angle. Time dependencies of electret response and hydrophobic recovery demonstrated qualitatively very similar behavior. Three distinct scale times of electric and hydrophobic restoring were extracted from the time dependencies of electric decay and hydrophobic recovery, extending from minutes to hundreds of minutes.
Plasma treatment increases the surface energy of condensed phases: solids and liquids. Two independent methods of the quantification of the influence imposed by a cold radiofrequency air plasma treatment on the surface properties of silicone oils (polydimethylsiloxane) of various molecular masses and castor oil are introduced. Under the first method the water droplet coated by oils was exposed to the cold air radiofrequency plasma, resulting in an increase of oil/air surface energy. An expression relating the oil/air surface energy to the apparent contact angle of the water droplet coated with oil was derived. The apparent contact angle was established experimentally. Calculation of the oil/air surface energy and spreading parameter was carried out for the various plasma-treated silicone and castor oils. The second method is based on the measurement of the electret response of the plasma-treated liquids.
The impact of the cold radiofrequency air plasma on the surface properties of silicone oils (polydimethylsiloxane) was studied. Silicone oils of various molecular masses were markedly hydrophilized by the cold air plasma treatment. A pronounced decrease of the apparent water contact angles was observed after plasma treatment. A general theoretical approach to the calculation of apparent contact angles is proposed. The treated liquid surfaces demonstrated hydrophobic recovery. The characteristic time of the hydrophobic recovery grew with the molecular mass of the silicone oil.
The probe intended for the characterization of cold plasma is introduced. The probe allows estimation of the Debye length of the cold plasma. The probe is based on the pronounced modification of surface properties (wettability) of polymer films by cold plasmas. The probe was tested with the cold radiofrequency inductive air plasma discharge. The Debye length and the concentration of charge carriers were estimated for various gas pressures. The reported results coincide reasonably with the corresponding values established by other methods. The probe makes possible measurement of characteristics of cold plasmas in closed chambers.
Plasma treatment increases the surface energy of condensed phases: solids and liquids.Two independent methods of the quantification of the influence imposed by a cold radiofrequency air plasma treatment on the surface properties of silicone oils (polydimethylsiloxane) of various molecular masses and castor oil are introduced.Under the first method the water droplet coated by oils was exposed to the cold air radiofrequency plasma, resulting in an increase of oil/air surface energy.An expression relating the oil/air surface energy to the apparent contact angle of the water droplet coated with oil was derived.The apparent contact angle was established experimentally.Calculation of the oil/air surface energy and spreading parameter was carried out for the various plasmatreated silicone and castor oils.The second method is based on the measurement of the electret response of the plasma-treated liquids.
