M.‐Pilar Marco

Institute of Advanced Chemistry of Catalonia

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J.‐Pablo Salvador

Institute of Advanced Chemistry of Catalonia

Francisco Sánchez‐Baeza

Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine

Roger Galvé

Institute of Advanced Chemistry of Catalonia

‪B

‪Damià Barceló

University of Almería

Javier Ramón‐Azcón

Institute for Bioengineering of Catalonia

Francisco Camps

Consejo Superior de Investigaciones Científicas

Daniel G. Pinacho

Universidad de La Sabana

Enrique Valera

University of Illinois Urbana-Champaign

Nuria Sanvicens

Consejo Superior de Investigaciones Científicas

Ana Sanchís

Institute of Advanced Chemistry of Catalonia

Cooperative Institutions

Consejo Superior de Investigaciones Científicas

163

Universitat de Barcelona

Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine

Universitat Autònoma de Barcelona

Instituto de Salud Carlos III

Institute of Advanced Chemistry of Catalonia

Instituto de Investigaciones Químicas y Ambientales de Barcelona

Centre National de la Recherche Scientifique

Centro de Investigación Biomédica en Red

Universidad Complutense de Madrid

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Conductimetric transducer array for the readout of low-density protein microarrays

Maria Mallén Diana Bonilla María Díaz‐González J.‐Pablo Salvador M.‐Pilar Marco

An electrical readout system of protein microarrays comprising arrays of gold interdigitated electrode pairs, which enabled impedance multiplexed detection of up to 36 biological events on the same substrate, and of polydimethylsiloxane (PDMS) microwells, is reported. Similarly to fluorescent readout counterparts, regular glass slides were applied for carrying out the microarray. However, unlike them, the presented system is compact and requires a simple and inexpensive instrumentation, thus being ideally suited for deployment of bioarray detection approaches. The system was based on the use of urease-based immunoassay formats applied to the detection of boldenone steroid achieving analytical results similar to those obtained by fluorescent scanner detection approaches.

Polydimethylsiloxane

Protein microarray

Instrumentation

Lab-on-a-Chip

10.1109/transducers.2013.6626992

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Citations (0)

A plasmonic biosensor array exploiting plasmon coupling between gold nanorods and spheres for domoic acid detection via two methods

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy (2021)

Joost L.D. Nelis J.‐Pablo Salvador M.‐Pilar Marco Christopher T. Elliott Katrina Campbell

Domoic Acid

Nanorod

10.1016/j.saa.2021.119473

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Citations (4)

Novel strategy for sulfapyridine detection using a fully integrated electrochemical Bio-MEMS: Application to honey analysis

Biosensors and Bioelectronics (2016)

Nadia El Alami El Hassani Abdoullatif Baraket Ernandes T. Tenório‐Neto Michael Lee J.‐Pablo Salvador

Sulfamerazine

Sulfapyridine

Amperometry

10.1016/j.bios.2016.08.083

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Citations (35)

Chapter 7 Immunoassays for environmental analysis

Techniques and instrumentation in analytical chemistry (2000)

Anna Oubiña Berta Ballesteros Pilar Bou Carrasco Roger Galvé Jordi Gascón

Immunochemical methods are powerful analytical techniques that can answer many questions concerning environmental contamination. This chapter provides an overview of the potential of immunoassays (IAs) as environmental analytical methods, describes the types and components of IAs, steps involved in their development and the criteria used for their development, and discusses various aspects related to the validation of immunochemical techniques. IA has proven to be a very useful technique for the screening of contaminating substances in environmental samples. The strength of this technique lies in the possibility to screen a large number of samples within a short time at low costs. Most of the IA tests are designed to be sensitive enough to assay water samples directly without concentration or clean-up steps. Both the availability of commercial IA test kits and the fact some IAs have already been included in the list of SW-846 methods of the Environmental Protection Agency (EPA) indicate that this technology is finding its place in the environmental area as an effective screening method to complement other analytical techniques. Future developments on immunosensor systems are a great promise for the future automation of these environmental immunodetection strategies.

Environmental Analysis

Complement

10.1016/s0167-9244(00)80013-x

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Citations (3)

Gel-based immunotest for simultaneous detection of 2,4,6-trichlorophenol and ochratoxin A in red wine

Analytica Chimica Acta (2010)

Natalia V. Beloglazova Irina Yu. Goryacheva T. Yu. Rusanova N. A. Yurasov Roger Galvé

10.1016/j.aca.2010.05.024

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Citations (22)

Bond Elasticity Controls Molecular Recognition Specificity in Antibody–Antigen Binding

Nano Letters (2013)

Anna Alemany Nuria Sanvicens Sara de Lorenzo M.‐Pilar Marco Félix Ritort

Force-spectroscopy experiments make it possible to characterize single ligand-receptor pairs. Here we measure the spectrum of bond strengths and flexibilities in antibody-antigen interactions using optical tweezers. We characterize the mechanical evolution of polyclonal antibodies generated under infection and the ability of a monoclonal antibody to cross-react against different antigens. Our results suggest that bond flexibility plays a major role in remodeling antibody-antigen bonds in order to improve recognition during the maturation of the humoral immune system.

Polyclonal antibodies

Force Spectroscopy

10.1021/nl402617f

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Citations (2)

A high-throughput screening (HTS) immunochemical method for the analysis of stanozolol metabolites in cattle urine samples

Journal of Chromatography B (2009)

J.‐Pablo Salvador Francisco Sánchez‐Baeza M.‐Pilar Marco

Stanozolol

10.1016/j.jchromb.2009.08.027

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Citations (11)

Evaluation of a Newly Developed Enzyme-Linked Immunosorbent Assay for Determination of Linear Alkyl Benzenesulfonates in Wastewater Treatment Plants

Environmental Science & Technology (2006)

Marinella Farré Javier Ramón‐Azcón Roger Galvé M.‐Pilar Marco ‪Damià Barceló

A recently developed enzyme-linked immunosorbent assay (ELISA) for the determination of linear alkyl benzenesulfonates (LAS) and long chain sulfophenyl carboxylates (SPCs) has been evaluated for its application in wastewater control analysis. This ELISA based on the use of polyclonal antibodies in an indirect format shows an IC50 of 28.1 +/- 3.2 microg L(-1) and a limit of detection (LOD) of 1.8 +/- 0.6 microg L(-1) in buffer. The assay uses antibodies raised through a pseudoheterologous immunization strategy using an equimolar mixture of two immunogens, N-(4-alkylphenyl)sulfonyl-3-aminopropanoic acid covalently coupled to keyhole limped hemocyanin (SFA-KLH) and sulfophenyl carboxylate 13C13 coupled to KLH (13C13-SPC-KLH). The immunizing haptens have been designed to address recognition versus two different epitopes of the LAS molecule. To assess the performance of this immunoassay in complex real samples, a cross reactivity study was carried out, and the possible interference of other surfactants commonly detected in wastewater, including nonylphenol ethoxylates (NPEOs), nonylphenol (NP), octylphenol (OP), and coconut fatty acid diethanol amides (CDEA), have been evaluated. Additionally, a study of the matrix effects of different types of wastewater was achieved. This ELISA has been evaluated and validated by measuring the LAS content of 22 samples collected from the influents and the effluents of six wastewater treatment plants (WWTP) located in Catalonia, Spain. A solid-phase extraction followed by liquid chromatography coupled to mass spectrometry detection (SPE-LC-MS) has been used as a validation method of the new ELISA test.

Nonylphenol

Hapten

Polyclonal antibodies

Solid phase extraction

10.1021/es060662+

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Citations (12)

13C Dynamic Nmr Studies on Restricted Rotation about C-N Bond in 2-Aryl-1-formyl-4-piperidones

Heterocycles (1989)

Mario Rubiralta M.‐Pilar Marco Miguel Feliz Ernest Giralt

AkKaci -The synthesis of l-formyl-2-(3-indolyl)-4-piperidone ( 7 ) .m -lformyl-2-(3-indolyl)-3-methyl-4-piperidone (E), and their 4.4-ethyleneacetals 5 and 6 is described.The introduction of a formyl group on the piperidone nitrogen

10.3987/com-89-5157

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Citations (17)

Phytoecdysteroid analysis by high-performance liquid chromatography-thermospray mass spectrometry

Journal of Chromatography A (1993)

M.‐Pilar Marco Francisco Sánchez‐Baeza Francisco Camps José Coll

Thermospray

Ecdysteroid

10.1016/0021-9673(93)83461-z

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Citations (17)