Objective: Tagetes erecta, marigold, has various ethnomedicinal uses. It has a wide variety of bioactive components such as polyphenols and flavonoids which show different bioactivities. The aim of the present study is to quantitatively estimate total phenolic content (TPC) and total flavonoid content (TFC) of different extracts of T. erecta flowers.Methods: Extraction was done by maceration process, sequentially from non-polar to polar. Chloroform, ethyl acetate, and methanol extracts of T. erecta flowers were subjected to preliminary phytochemical screening. The extracts were analyzed for TPC and TFC using gallic acid and rutin as standard, respectively.Result: Phytochemical screening of different extracts showed the presence of carbohydrates, flavonoids, phenolics, fats, and oils. TPC and TFC in extracts of T. erecta varies with solvents. The study revealed that methanolic extract possesses the highest phenolic content, 49.76±0.29 mg gallic acid equivalents/g extract, and also maximum flavonoid content, 13.43±0.43 mg RE/g extract, among the three extracts.Conclusion: Higher value of phenolics and flavonoid indicates higher antioxidant activity. The present study revealed that methanolic extract has the highest phenolic and flavonoid content. This indicates that the flowers may possess a good antioxidant property and further research could be carried out.
Tagetes erecta (T. erecta) is an herbaceous species, having wide ethno medicinal and traditional uses. It demonstrates different pharmacological activities like antioxidant, antimicrobial and anti depressant. The present study deals with the estimation of antioxidant properties of four solvent extracts of flowers of T. erecta. The study aims to discover potent extracts as a novel source of natural antioxidant. The crude extracts; Petroleum ether (PE), Chloroform (CF), Ethyl Acetate (EA) and Methanol (MET) were screened for their free radical scavenging capacity and reducing powers using DPPH, Superoxide and Reducing power assay. Ascorbic acid was used as standard. All the extracts show considerable antioxidant potential. However, methanolic extract can strongly scavenge DPPH and superoxide radical with significant IC50 value range 30.08 ± 0.98 µg/mL and 64.22±0.04 µg/mL respectively. The IC50 value of the extracts follows the order; MET less than EA less than PE less than CF. Lowest IC50 value indicates highest antioxidant potential. The reducing power of extracts were in the order; CF less than PE less than EA less than Met
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