A simple and precise novel stability-indicating method for the simultaneous estimation of tezacaftor and ivacaftor in combined tablet dosage form was developed and validated using reversed-phase high-performance liquid chromatography (RP-HPLC). The method is being reported for the first time and includes an estimation of degradation products produced post-stress conditions without any extraction or derivatization. The chromatographic separation of the drugs was achieved with a Symmetry Shield RP18 Column (100 Å, 5 μm, 4.6 mm × 250 mm) using a mixture of buffer, methanol and acetonitrile (42:27:31 v/v/v) as mobile phase. The buffer used in mobile phase contained 35 mM potassium dihydrogen phosphate, and its pH was adjusted to 7.0 ± 0.02 with 20% orthophosphoric acid. The instrument was set at flow rate of 1.2 mL min-1 at ambient temperature and the wavelength of UV-visible detector at 275 nm. The developed method could be suitable for the quantitative determination of these drugs in pharmaceutical preparations and also for quality control in bulk manufacturing. Stress testing was performed to prove the specificity. No interference was observed from its stress degradation products. The statistical analysis was done by using F-test and t-test at 95% confidence level.
Objective: To perform the Phytochemical Screening & Anti-inflammatory activity of Murraya Koenigii & Ficus Lacor roots in Albino Wistar rats.
Methods: The different Pharmacognostical parameters were evaluated as per standard procedure. The crude drug was evaluated for organoleptic properties shape, size, color, odor, taste. Preliminary Phytochemical Screening was carried out & finally Anti-inflammatory activity was evaluated by adopting different methods.
Results: The extracts showed a marked Anti-inflammatory effect. The FLET fraction from Ficus lacor aerial roots showed maximum inhibition (75%) of Carrageenan induced edema (Table 3.12B), followed by FLPE and MKCF from Murraya koenigii roots (74 % and 70% approx) (p <0.001 for all). The inhibition of inflammation was comparable for all the fractions for any change in extent & percentage of inhibition at 30 min, 1hr, 2hr and 3hr. The anti-inflammatory effect induced via indomethacin gradually increased and reached at higher level (80.8%) after 3 hrs. It was maintained up to six hours. For, MKPE the inhibition was observed to be maximum at the end of 2 hrs and then tapered. MKEA, FLEA and FLCF showed minimum response that was constant throughout and insignificant. MKPE was slightly significant (55.1 %, p<0.05) which was more effective.
Conclusion: The results of the study indicate that the extract of Murraya koenigii roots and Ficus lacor possesses strong Anti-inflammatory activity. This study also describes therapeutic effect of Murraya koenigii roots and Ficus lacor aerial roots in inflammation and arthritis which will give a new direction for the future scientific research.
Ayurveda the ancient science of medicine describes various herb preparations that achieve the hastening of bone healing. Harjor showed clinical efficacy in the treatment of fractures.The comparative evaluation of herbal agents as osteogenic agents in mandibular fractures.The patients were divided into four groups. Group 1: Osteoseal; Group 3: Harjor (Cissus quadrangularis); Group 2: Moringa (Moringa Oleifera); Group 4: Placebo.Pain, Swelling, Tenderness, Mobility reduction is maximum in Osteoseal group and minimum in Placebo. There was an increase in the serum calcium and phosphorus level at different follow-ups in each groups but there was a decrease in the placebo group. Ca, Ca+, Phosphrous increase was maximum in the group 1.
Pharmaceutical analysis is one of the most challenging fields of analytical chemistry. Pharmaceutical analysts carry out the qualitative and quantitative control of APIs and drug products and also develop and validate appropriate methods. One of my main goals was to develop modern, rapid, precise and reproducible, but also cost-effective HPLC assay methods which are generally available and applicable for most users. The aim of this work was to develop LC methods for both compounds. The assay of erythromycin by LC offers several advantages, such as high specificity, the possibility of determining and quantifying impurities and degradation products, and improved accuracy. The developed methods were validated. My whole work containing following plan of work as Selection of drug, Review Literature, FITR of both drugs and Mixture, Preparation of standard solutions, Preparation of sample of pure drug in Standard solution, Method development by HPLC (as Selection of solvents to be used as diluents and mobile phase, Selection of wavelength, Selection of mobile phase and Selection of chromatographic conditions) Preparation of Mobile phase, Preparation of standard calibration curve combination of drug, Optimization of HPLC condition using box behnken design. Validation of analytical method following parameters as per ICH guidelines. (i). System suitability (ii). Linearity and range (iii). Specificity (iv).Accuracy and precision (v). Limits of detection (LOD) and Quantitation (LOQ). (vi). Selectivity and (vii).Robustness.
A simple, fast, accurate and precise RP-HPLC method were developed and validated for the estimation of Azithromycin & Benzoyl peroxide per ICH guidelines. Potassium dihydrogen phosphate and Acetonitrile (50:50) are commonly used as solvents. The method was developed in Eclipse C18 column (Waters XTerra®, 4.6Χ250 mm, particle 5μ) with Potassium dihydrogen phosphate and Acetonitrile are commonly used solvents in RP -HPLC having low UV cut- off of 200-400 nm respectively. In RP-HPLC method was found to be linear in the range of Azithromycin/Benzoyl peroxide is 1-5μg/ml with a correlation coefficient value of 0.99. The accuracy studies of RP-HPLC method was performed at three different levels, i.e., 80%, 100%. The limit of detection (LOD) and Limit of Quantification (LOQ) for Azithromycin 0.009μg/ml & 0.028μg/ml and for Benzoyl peroxide 0.015μg/ml & 0.046μg/ml is to be were found. The Percentage RSD should not be more than 2 which indicate the accuracy and precision of the method. Hence the method was robust.Thus this shows that the method is capable to give a good detector response, the recovery calculated was within the range of 98% to 101% of the specification limits. Hence the method was a rapid tool for routine analysis of Azithromycin/Benzoyl peroxide in the bulk and in the pharmaceutical dosage form.
The hydro-alcoholic extracts of Nyctanthes arbortristis were evaluated for anthelmintic activity using adult earthworms; the leaves extract of Nyctanthes arbortristis exhibited a dose-dependent inhibition of spontaneous motility (paralysis) and evoked responses to pin-prick. With lower doses the effects were comparable with that of albendazole. However, there was no final recovery in the case of worms treated with hydro-alcoholic extract. The result showed that the hydro- alcoholic extract possessed wormicidal activity and thus, may be useful as an anthelmintic.
Background and objectives: As an indispensable dietary component, fresh fruits and vegetables, which are often consumed raw, have been reported to harbor large microbial populations often culminating in enteric disease outbreaks.The present study aimed to assess the microbial quality of coriander and mint and find the efficacy of potassium permanganate (KMnO 4) in effectively removing such microbes. Materials and methods:Ten grams each of fresh, unstored green coriander (n = 50) and mint (n = 50) samples were examined for their parasitic and bacterial content before and after washing with tap water and with an aqueous solution of 0.001% KMnO 4. The different species of bacteria, as well as their main burden in these herbs, were also estimated along with the parasites they harbored.Results: Only 22% unwashed herbs harbored parasites which were reduced to a significant number on washing with tap water.One hundred percent of the herb samples before washing, and 98% of tap water washed herb samples were contaminated with bacteria.A total of 33 different species of bacteria were isolated, from members of the family Enterobacteriaceae (56.3%)Pseudomonadaceae (15.6%),Vibrionaceae (9.4%,), and Acinetobacteriaceae (6.2%).These bacteria were significantly removed after treatment with KMnO 4 . Conclusion:The study reveals the presence of harmful enteric bacteria and parasites in fresh green coriander and mint which are liable to cause enteric diseases.We recommend that all the fresh produce which is consumed raw should be treated with 0.001% KMnO 4 after tap water washing to render them safe for consumption.
A simple and precise stability indicating method for the simultaneous estimation of dapagliflozin and saxagliptin in combined tablet dosage form was developed and validated using RP-HPLC.
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