Ivana Serraino

University of Messina

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Co-Authors

Salvatore Cuzzocrea

Saint Louis University

Achille P. Caputi

University of Messina

Emanuela Mazzon

University of Messina

Laura Dugo

University of Messina

Rosanna Di Paola

University of Messina

Domenico Britti

Magna Graecia University

Christoph Thiemermann

William Harvey Research Institute

Tiziana Genovese

University of Messina

Lidia Sautebin

University of Naples Federico II

Angela De Sarro

University of Messina

Cooperative Institutions

University of Messina

William Harvey Research Institute

University of Naples Federico II

Queen Mary University of London

Centro Neurolesi Bonino Pulejo

University of Florence

Federico II University Hospital

Magna Graecia University

Azienda Ospedaliera Universitaria Policlinico "G. Martino"

St Bartholomew's Hospital

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Absence of endogeneous interleukin-10 enhances the evolution of murine type-II collagen-induced arthritis.

PubMed (2002)

Salvatore Cuzzocrea Emanuela Mazzon Laura Dugo Ivana Serraino Domenico Britti

Interleukin-10 (IL-10) exerts a wide spectrum of regulatory activities in the immune and inflammatory response. The aim of this study was to investigate the role of endogenous IL-10 in the modulation of the inflammatory response in mice subjected to collagen-induced arthritis. Collagen-induced arthritis (CIA) was induced in mice lacking the gene for IL-10 (IL-10 "knock-out", IL-10KO) and in wild-type control (IL-10WT) mice by an intradermal injection of 100 mul of the emulsion (containing 100 mug of bovine type II collagen) (CII) and complete Freund's adjuvant (CFA) at the base of the tail. On day 21, a second injection of CII in CFA was administered. IL-10 wild type (WT) mice developed an erosive, hind paw arthritis when immunised with CII in CFA. Macroscopic clinical evidence of CIA first appeared as peri-articular erythema and oedema in the hind paws. The incidence of CIA was 100% by day 27 in the CII-challenged IL-10WT. The severity of CIA progressed over a 35-day period, with radiographic evaluation revealing focal resorption of bone. The histopathology of CIA included erosion of the cartilage at the joint margins. IL-10KO mice experienced higher rates of clinical signs and more severe knee and paw injury as compared to IL-10WT. The degree of oxidative and nitrosative damage was significantly higher in IL-10KO mice than in wild-type littermates, as indicated by elevated malondialdehyde levels and formation of nitrotyrosine and poly (ADP-ribose) synthetase (PARS). Plasma levels of the proinflammatory cytokines, tumour necrosis factor, interleukin-1beta and interleukin-6 were also greatly enhanced in comparison to wild-type mice. These data demonstrate that endogenous IL-10 exerts an anti-inflammatory role during chronic inflammation and tissue damage associated with collagen-induced arthritis, possibly by regulating neutrophil recruitment, and the subsequent cytokine and oxidant generation.

Type II collagen

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Protective Effects of Anthocyanins from Blackberry in a Rat Model of Acute Lung Inflammation

Free Radical Research (2003)

Antonietta Rossi Ivana Serraino Paola Dugo Rosanna Di Paola Luigi Mondello

Anthocyanins are a group of naturally occuring phenolic compounds related to the coloring of plants, flowers and fruits. These pigments are important as quality indicators, as chemotaxonomic markers and for their antioxidant activities. Here, we have investigated the therapeutic efficacy of anthocyanins contained in blackberry extract (cyanidin-3-O-glucoside represents about 80% of the total anthocyanin contents) in an experimental model of lung inflammation induced by carrageenan in rats. Injection of carrageenan into the pleural cavity elicited an acute inflammatory response characterized by fluid accumulation which contained a large number of neutrophils as well as an infiltration of polymorphonuclear leukocytes in lung tissues and subsequent lipid peroxidation, and increased production of nitrite/nitrate (NOx) and prostaglandin E2 (PGE2). All parameters of inflammation were attenuated in a dose-dependent manner by anthocyanins (10, 30 mg kg-1 30 min before carrageenan). Furthermore, carrageenan induced an upregulation of the adhesion molecule ICAM-1, nitrotyrosine and poly (ADP-ribose) synthetase (PARS) as determined by immunohistochemical analysis of lung tissues. The degree of staining was lowered by anthocyanins treatment. Thus, the anthocyanins contained in the blackberry extract exert multiple protective effects in carrageenan-induced pleurisy.

Carrageenan

ABTS

10.1080/1071576031000112690

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Citations (186)

5-Lipoxygenase knockout mice exhibit a resistance to pleurisy and lung injury caused by carrageenan

Journal of Leukocyte Biology (2003)

Salvatore Cuzzocrea Antonietta Rossi Ivana Serraino Emanuela Mazzon Rosanna Di Paola

In the present study, by comparing the responses in wild-type (WT) mice and mice lacking [knockout (KO)] the 5-lipoxygenase (5-LO), we investigated the role played by 5-LO in the development of acute inflammation. When compared with carragenan-treated 5-LOWT mice, 5-LOKO mice, which had received carrageenan, exhibited a reduced degree of pleural exudation, polymorphonuclear cell migration. Lung myeloperoxidase activity, an index of neutrophil infiltration, was significantly reduced in 5-LOKO mice in comparison with 5-LOWT. Lung-tissue sections from carrageenan-treated 5-LOWT mice showed positive staining for intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), P-selectin, and E-selectin, which were mainly localized around vessels. The intensity and degree of ICAM-1, VCAM-1, P-selectin, and E-selectin were markedly reduced in tissue section from carrageenan-5-LOKO mice, which also improved the histological status of the inflamed lungs. Taken together, our results clearly demonstrate that 5-LO modulates neutrophil infiltration in the acute lung inflammation.

Pleurisy

Carrageenan

Infiltration (HVAC)

Knockout mouse

10.1189/jlb.1002477

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Citations (34)

Regulation of prostaglandin generation in carrageenan-induced pleurisy by inducible nitric oxide synthase in knockout mice

Life Sciences (2003)

Antonietta Rossi Salvatore Cuzzocrea Emanuela Mazzon Ivana Serraino Angela De Sarro

Carrageenan

Pleurisy

Exudate

10.1016/s0024-3205(02)02375-5

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Citations (10)

GPI 6150, a poly (ADP-ribose) polymerase inhibitor, exhibits an anti-inflammatory effect in rat models of inflammation

European Journal of Pharmacology (2001)

Emanuela Mazzon Ivana Serraino Jiahe Li Laura Dugo Achille P. Caputi

PARP inhibitor

Zymosan

10.1016/s0014-2999(01)00809-3

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Citations (43)

Pyrrolidine dithiocarbamate attenuates the development of acute and chronic inflammation

British Journal of Pharmacology (2002)

Salvatore Cuzzocrea Prabal K. Chatterjee Emanuela Mazzon Laura Dugo Ivana Serraino

The nuclear factor‐κB (NF‐κB) is a transcription factor which plays a pivotal role in the induction of genes involved in physiological processes as well as in the response to injury and inflammation. Dithiocarbamates are antioxidants which are potent inhibitors of NF‐κB. We postulated that pyrrolidine dithiocarbamate (PDTC) would attenuate inflammation. In the present study we investigate the effects of PDTC in animal models of acute and chronic inflammation (carrageenan‐induced pleurisy and collagen‐induced arthritis). We report here for the first time that PDTC (given at 100, 30 or 10 mg kg −1 i.p. in the pleurisy model or at 10 mg kg −1 i.p. every 48 h in the arthritis model) exerts potent anti‐inflammatory effects (e.g. significant reduction of (A) pleural exudate formation, (B) polymorphonuclear cell infiltration, (C) lipid peroxidation, (D) inducible nitric oxide synthase (iNOS) activity and nitric oxide production (E) plasma and pleural exudates levels of interleukin‐1β and tumour necrosis factor‐α, (F) histological injury and (G) delayed development of clinical indicators). Furthermore, PDTC reduced immunohistochemical evidence of (A) formation of nitrotyrosine, (B) activation of poly (ADP‐ribose) polymerase (PARP), (C) expression of iNOS and (D) expression of cyclo‐oxygenase‐2 (COX‐2) in the lungs of carrageenan‐treated mice and in the joints from collagen‐treated mice. Additionally, Western blotting and immunohistochemical analysis of lung tissue revealed that PDTC prevented degradation of IKB‐α and translocation of NF‐κB from the cytoplasm into the nucleus. Taken together, our results clearly demonstrate that prevention of the activation of NF‐κB by PDTC reduces the development of acute and chronic inflammation. Therefore, inhibition of NF‐κB may represent a novel approach for the therapy of inflammation. British Journal of Pharmacology (2002) 135 , 496–510; doi: 10.1038/sj.bjp.0704463

Pyrrolidine dithiocarbamate

Nitrotyrosine

10.1038/sj.bjp.0704463

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Citations (209)