Mokhamad Fahrudin

IPB University

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Ni Wayan Kurniani Karja

IPB University

Junko Noguchi

Institute of Agrobiological Sciences

Takashi Nagai

Tokushima University

Takeshige Otoi

Tokushima University

Hiroyuki Kaneko

National Agriculture and Food Research Organization

Arief Boediono

IPB University

Kazuhiro Kikuchi

Institute of Agrobiological Sciences

T. Somfai

Institute of Agrobiological Sciences

Manabu Ozawa

Systems Biology Institute

Katsuhiko Ohnuma

Institute of Agrobiological Sciences

Cooperative Institutions

IPB University

Yamaguchi University

Institute of Agrobiological Sciences

Tokushima University

National Research and Innovation Agency

Kyushu University

National Agriculture and Food Research Organization

Detská Fakultná Nemocnica s Poliklinikou

Institute of Livestock and Grassland Science

Universitas Gadjah Mada

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Antioxidant Activity and Total Phenolic Content of Stingless Bee Propolis from Indonesia

Journal of Apicultural Science (2019)

Al Mukhlas Fikri Ahmad Sulaeman Sri Anna Marliyati Mokhamad Fahrudin

Abstract The aim of this study was to investigate antioxidant activity, total phenolic compounds and flavonoids of propolis from three provinces of Indonesia with two methods of extraction. Stingless bee propolis from Banten, South Kalimantan, and South Sulawesi was prepared through ultrasound-assisted extraction with water and ethanol solvents, separately. Antioxidant activity was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Total phenolic and flavonoid contents were analyzed by Folin-Ciocalteu colorimetric and aluminum chloride (AlCl 3 ) methods, respectively. The result showed that the antioxidant activity expressed as IC 50 of Indonesian propolis ranged from 452.52 to 1027.29 mg/L. Ethanol extracts showed higher antioxidant activity than water extracts. Total phenolic and flavonoids content detected in the samples ranged from 10.00 to 28.65 mg/g GAE and 0.76 to 3.39 mg/g QE, respectively. The ethanol extract of propolis from South Kalimantan had the highest total phenolic contents, while that from South Sulawesi possessed the highest total flavonoids.

Propolis

Stingless bee

10.2478/jas-2019-0012

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Citations (40)

Effect of Cycloheximide on In Vitro Development of Electrically Activated Feline Oocytes

Journal of Reproduction and Development (2005)

Ni Wayan Kurniani Karja Takeshige Otoi Masako Murakami Pimprapar Wongsrikeao Agung Budiyanto

This study was conducted to improve parthenogenetic development in vitro of feline oocytes following a combined activation treatment of electrical stimulation and cycloheximide. In vitro matured (IVM) oocytes were stimulated electrically by a DC electrical pulse of 2 kV/cm for 50 micros. The stimulated oocytes were then incubated in MK-1 medium with or without cycloheximide and subsequently cultured in vitro for 6 days. No significant differences were observed between the two groups with respect to the proportions of cleavage, development to the morula stage, and the cell number of blastocysts. However, exposure of electrically stimulated oocytes to cycloheximide significantly increased the rate of development of the stimulated oocytes into the blastocyst stage compared with oocytes stimulated by electrical stimulation alone (31.0% vs 6.7%). The results from the present study suggested that a single electrical stimulation was insufficient to activate the IVM cat oocytes at 24 h of maturation and that exposure to cycloheximide following electrical stimulation improved the efficacy of the parthenogenetic development of domestic cat oocytes.

Parthenogenesis

In vitro maturation

Oocyte activation

10.1262/jrd.17056

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Relationship between Nucleus Swelling and Development Competence of Bovine Cloned Embryos Reconstructed by Enucleated Oocytes with Serum-starved or Serum-fed Fetal Somatic Cells

Indonesian Journal of Biotechnology (2015)

Mokhamad Fahrudin Ni Wayan Kurniani Karja Tatsuyuki SUZUKI

This study was conducted to examine the occurrence of nuclear remodeling (nucleus swelling) and its effectson the subsequent in vitro development of bovine embryos reconstructed by serum-starved and serum-fed somaticcells. Results from this study demonstrated that all of the reconstructed embryos that received serum-starved andserum-fed somatic cells exhibited condensed-nuclei. More than 90% of the transferred nuclei exhibited nuclearenvelope breakdown and premature chromatin condensation which clearly distinct from an intact nucleus. Therewas no significant difference on the degree of nucleus swelling in SS-NT embryos or SF-NT embryos, indicatingthat either serum-starved or confluent somatic cell lines could be reprogrammed by the recipient cytoplasmenvironments in similar pattern. Although the fusion rate was not significantly different among the groups, theproportion of SS-NT embryos which developed to the 2- to 4-cell stage (89.7%) and to the 8- to 16-cell stage (74.7%)was significantly higher than that of SF-NT embryos. Whereas, the proportion of reconstructed embryos thatdeveloped to the morula and blastocyst stages were not significantly different among the groups. Results of thesestudies demonstrate that reconstructed embryos, which received either serum-starved or serum-fed confluentsomatic cells, showed similar developmental competence to the blastocyst stage.Keywords: nuclear transplantation technique-somatic cells-nucleus swelling

10.22146/ijbiotech.7553

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Temperature of Eggshell, Weight Loss, and Air Sac on Hatched Local Duck Eggs During Incubation

Buletin Peternakan (2018)

Yusuf Kurniawan Rukmiasih Rukmiasih Mokhamad Fahrudin Peni Soeprapti Hardjosworo

The study was conducted to analyze the characteristic of eggs (temperature of eggshell, weight loss, and air sac alteration) at various hatching period of local duck hatched (H) and unhatched (UH) at the final observation, and to find out the effective time for estimating the life of embryo during the end of incubation. A total of 146 eggs were incubated and observed between 1 and 25 days of incubation (DOI). The results of weight loss and air sac change showed a significant difference (P<0,05) between H and UH eggs on 7 to 25 DOI, while the temperature of eggshell was only different on 25 DOI. The average characteristic of H group (temperature of eggshell, weight loss, and air sac alteration) on 25 DOI was recorded 38,46oC, 11,84%, and 51,03%, respectively. It can be concluded that 3 characteristics of eggs influence hatchability of local duck. Weight loss and air sac alteration parameters can be applyed to estimate the hatched eggs between 7 and 25 DOI, but the temperature of eggshell can be administrated after 25 DOI.

Air cell

Incubation period

10.21059/buletinpeternak.v42i3.31279

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Citations (2)

Cryopreservation and in vitro fertilizing ability of ram spermatozoa from cold storage epididimides

Mokhamad Fahrudin Ni Wayan Kurniani Karja Mohamad Agus Setiadi

10.14882/jrds.105.0_1099

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CHARACTERISTICS AND FERTILITY OF SUMATRAN TIGER SPERMATOZOA CRYOPRESERVED WITH DIFFERENT SUGARS.

PubMed (2016)

Ni Wayan Kurniani Karja Mokhamad Fahrudin Mohamad Agus Setiadi Ligaya ITA Tumbelaka R Sudarwati

Cryopreservation of semen is one of the most important methods for the preservation of endangered tigers.This study evaluated the effects of sugar supplementation on the cryosurvival of spermatozoa from Sumatran tigers (Panthera tigris sumatrae).The post-thaw characteristics and fertility of spermatozoa cryopreserved with different sugars (glucose, lactose, and trehalose) were evaluated using heterologous in-vitro fertilisation with cat oocytes.All parameters of post-thaw spermatozoa significantly decreased as compared with those of fresh spermatozoa. The index of sperm motility for semen cryopreserved with lactose was significantly higher than that for semen cryopreserved with trehalose. The percentage of total fertilisation for tiger spermatozoa cryopreserved with trehalose was significantly lower than that for control cat spermatozoa.Our findings indicated that supplementation with lactose or glycerol as the main sugar in the egg yolk extender resulted in a better motility and fertility potential for post-thawed spermatozoa.

Semen cryopreservation

Cryoprotectant

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Citations (6)

Endonezya Propolis'in Hamile Farelerde İmmünomodülatör Etkisi: Bir Ön Sonuç

Uludağ Arıcılık Dergisi (2022)

Al Mukhlas Fikri Ahmad Sulaeman Sri Anna Marliyati Mokhamad Fahrudin Ekowati Handharyani

Propolis has been widely accepted to exhibit immunomodulatory activity. However, the activity during pregnancy has not been investigated yet. This study was a preliminary study that aimed to analyze the effect of several types of Indonesian propolis on the histological changes of maternal mice spleen. A total of 25 pregnant mice were divided into 5 groups, control (1% Tween 80) group, low (380 mg/kg) and high dose (1400 mg/kg) ethanol extract of South Sulawesi propolis groups, and low (380 mg/kg) and high dose (1400 mg/kg) water extract of Banten propolis groups. Propolis samples were administered daily during pregnancy. At day 18 of gestation, the mice were sacrificed to obtain spleen which was used for histological evaluation using hematoxylin and eosin staining. The number and diameter of white pulp were observed under the 10x magnifying of microscope. The results showed that all propolis extracts at low dose significantly increased the number of white pulp (p < 0.05). However, an increase in the diameter was found not significant in all propolis administered groups. This study suggests that Indonesian propolis may modulate maternal immune system.

Propolis

White pulp

Histology

Positive control

10.31467/uluaricilik.1098059

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63 PARTHENOGENETIC DEVELOPMENT OF PORCINE OOCYTES RECONSTRUCTED BY CENTRIFUGATION AND ELECTROFUSION AFTER METAPHASE-II CHROMOSOME TRANSFER

Reproduction Fertility and Development (2006)

N. Maedomari Kazuhiro Kikuchi Mokhamad Fahrudin Michiko Nakai Manabu Ozawa

Metaphase-II chromosome transfer (M-II transfer) is considered to be a useful technique for studying nucleus–cytoplasm relationships, or for generating oocytes with good developmental ability after transfer of the nucleus to the cytoplasm. The reconstructed oocytes carry the original genomic information within the metaphase chromosomes from the donor oocytes. The objective of the present study was to evaluate the parthenogenetic developmental ability of porcine M-II transferred oocytes. In vitro maturation was carried out as reported previously (Kikuchi et al. 2002 Biol. Reprod. 66, 1033–1041). After culture for 44 h, cumulus cells were removed by hyaluronidase treatment and gentle pipetting. Oocytes that had extruded the first polar body were selected and centrifuged at 13 000g for 9 min to stratify the cytoplasm. The zonae pellucidae were removed after exposure to pronase, and zona-free oocytes were layered on a 300 �L discontinuous gradient (100 �L each of 45%, 30%, and 7.5%) of Percoll in TCM-HEPES supplemented with 5 �g mL-1 cytochalasin B. After centrifugation of the oocytes on the gradient in microcentrifuge tubes at 6000g for 20 s, fragmented cytoplasm with an equal volume was obtained, stained with Hoechst 33342, and classified as cytoplasm with or without chromosomes by observation with a fluorescence microscope. One fragmented cytoplasm with chromosomes and 2 fragmented cytoplasms without chromosomes were fused by electric stimulation with a single DC pulse (1.5 kV cm-1, 20 �s) and cultured temporarily for 1 h. The reconstructed oocytes were then stimulated again to induce parthenogenetic activation (0.8 kV cm-1, 30 �s, 2 DC pulses) (treatment group). Zona-free mature oocytes that had not been subjected to reconstruction were activated as a control group. The oocytes in both groups were treated with 5 �g mL-1 cytochalasin B for 2 h, and then cultured for 6 days in media (Kikuchi et al. 2002) using the WOW system (Gabor et al. 2000 Mol. Reprod. Dev.). The blastocyst formation rate in the control group (22.9 � 5.5%) was significantly higher (P < 0.05; ANOVA and PLSD-test) than that in the treatment group (7.6 � 1.8%). The total cell number per blastocyst in the control group (28.7 � 4.6) was significantly higher (P < 0.05) than that in the treatment group (16.7 � 1.0). These results suggest that reconstructed porcine oocytes following M-II transfer by centrifugation and electrofusion can develop to the blastocyst stage in vitro. This technique enables transfer of the nucleus to cytoplasm with good developmental ability without the use of a micro-manipulation system.

Pronucleus

Percoll

Electrofusion

Cytochalasin B

Polar body

Pipette

10.1071/rdv19n1ab63

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Allotransplantasi testis mencit muda sebagai upaya preservasi gonad in vivo

Jurnal Ilmu Pertanian Indonesia (2007)

Wahono Esthi Prasetyaningtyas Kusdiantoro Mohamad Mokhamad Fahrudin Ita Djuwita Srihadi Agungpriyono

Cancer disease are not detected only at adult but also at young age. One therapy for cancer diseases is chemotherapy and radiation, that give a side effect of infertility in the gonad, therefore, it is necessary to preserve the gonad. Sperm collection from adult is easy but not from the young patients. Keyword: transpalantation, testis, mencit muda, spermatogenesis

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Development to the blastocyst stage, the oxidative state, and the quality of early developmental stage of porcine embryos cultured in alteration of glucose concentrations in vitro under different oxygen tensions

Reproductive Biology and Endocrinology (2006)

Ni Wayan Kurniani Karja Kazuhiro Kikuchi Mokhamad Fahrudin Manabu Ozawa T. Somfai

Recent work has shown that glucose may induce cell injury through the action of free radicals generated by autooxidation or through hypoxanthine phosphoribosyltransferase inhibition. The effect of glucose during early in vitro culture (IVC) period of porcine embryos on their developmental competence, contents of reactive oxygen species (ROS) and glutathione (GSH), and the quality of the blastocysts yielded was examined.In vitro matured and fertilized porcine oocytes were cultured for the first 2 days (Day 0 = day of fertilization) of IVC in NCSU-37 added with 1.5 to 20 mM glucose (Gluc-1.5 to -20 groups) or pyruvate and lactate (Pyr-Lac group). The embryos in all groups were cultured subsequently until Day 6 in NCSU-37 with 5.5 mM added glucose. The ROS and GSH level were measured at Day 1 and 2. DNA-fragmented nuclei and the total cell numbers in blastocyst were evaluated by TUNEL-staining at Day 6.Under 5% oxygen the blastocyst rates and total cell numbers in the blastocysts in all glucose groups were significantly lower than that in the Pyr-Lac group. Similar result in blastocyst rate was found under 20% oxygen (excluding the Gluc-10 group), but total cell numbers in the blastocysts was similar among the groups. At both oxygen tensions, the H2O2 levels of Day 1 embryos in all glucose groups were significantly higher than that in the Pyr-Lac group, while only the Gluc-1.5 group of Day 2 embryos showed a significantly higher H2O2 level than that in the Pyr-Lac group. The GSH contents of either Day 1 or Day 2 embryos developed under 5% oxygen were similar among the groups. Only the content of Day 2 embryos in 1.5 mM group was significantly lower than the embryos in the Pyr-Lac group under 20% oxygen. Total cell numbers in the blastocysts (except in the Gluc-20 group) were significantly lower in the embryos cultured under 20% oxygen than 5% oxygen. Only the Gluc-20 blastocysts developed under 5% oxygen showed significantly higher DNA fragmentation rate than those of Pyr-Lac blastocysts.These results show that a decrease in developmental ability of embryos cultured by use of glucose instead of pyruvate and lactate after the ferilization may be due to the rise in ROS generation in Day 1 embryos. Moreover, results from this study suggest that the concentration of glucose in the medium that can be used by the Day 1-2 embryos is limited to 3.5 mM and exposure to higher glucose concentrations does not improve embryo development.

10.1186/1477-7827-4-54

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Citations (45)