H. Paco Kang

Cell Technology (China)

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Michael D. Gershon

Columbia University

Guangchun Jin

Columbia University

Alka Kolhe

Cancer Research Center

Heon Goo Lee

Columbia University

Karan Nagar

Carisma Therapeutics (United States)

Yiling Si

Chinese PLA General Hospital

Tracy C. Grikscheit

Children's Hospital of Los Angeles

Samuel Asfaha

Western University

Thaddeus S. Stappenbeck

Cleveland Clinic

Andrew S. Giraud

Murdoch Children's Research Institute

Cooperative Institutions

Columbia University

Columbia University Irving Medical Center

Cancer Research Center

Heidelberg University

National Center on Addiction and Substance Abuse at Columbia University

Harvard University

University of Michigan–Ann Arbor

Chinese Academy of Medical Sciences & Peking Union Medical College

Washington University in St. Louis

Howard Hughes Medical Institute

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Gremlin 1 Identifies a Skeletal Stem Cell with Bone, Cartilage, and Reticular Stromal Potential

Cell (2015)

Daniel L. Worthley Michael Churchill Jocelyn Compton Yagnesh Tailor Meenakshi Rao

10.1016/j.cell.2014.11.042

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Citations (587)

Gremlin 1 Identifies a Skeletal Stem Cell with Bone, Cartilage, and Reticular Stromal Potential

PMC (2015)

Daniel L. Worthley Michael Churchill Jocelyn Compton Yagnesh Tailor Meenakshi Rao

Amniotic stem cells

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Serum and xeno‐free, chemically defined, no‐plate‐coating‐based culture system for mesenchymal stromal cells from the umbilical cord

Cell Proliferation (2016)

Xiaoyun Wu H. Paco Kang Xuemin Liu Jin Gao Kuijun Zhao

Umbilical cord mesenchymal stromal cells (UCMSCs) can be considered to become a new gold standard for MSC-based therapies. A serum and xeno-free, chemically defined and no-plate-coating-based culture system will greatly facilitate development of robust, clinically acceptable bioprocesses for reproducibly generating quality-assured UCMSCs.In this study, we report for the first time, such a serum-free, xeno-free, completely chemically defined and no-plate-coating-based culture system for the isolation and expansion of UCMSCs, whose biological characteristics were evaluated and compared with serum-containing medium (SCM) methods.This culture system not only supported UCMSC primary cultures but also allowed for their expansion at low seeding density. Compared to SCM, UCMSCs in SFM exhibited (i) higher proliferative and colony-forming capacities; (ii) distinctly different morphologies; (iii) similar phenotype; (iv) similar pluripotency-associated marker expression; (v) superior osteogenic, but reduced adipogenic differentiation capacitities. In addition, UCMSCs cultured in SFM retained similar immunomodulatory properties to those in SCM.Our findings demonstrate the feasibility of isolating and expanding UCMSCs in a completely serum-free, xeno-free, chemically defined and no-plate-coating-based culture system and represent an important step forward for development of robust, clinically acceptable bioprocesses for UCMSCs. Further, this provides a superior study platform for UCMSCs biology in a controlled environment.

10.1111/cpr.12279

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Citations (32)

Zoledronate increases enrichment, activation and expansion of natural killer cells from umbilical cord blood

Human Cell (2018)

Zhijie Ma Yun-Hong Wang H. Paco Kang Xiaoyun Wu

Cord blood

Placenta cord banking

10.1007/s13577-018-0213-2

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Citations (2)