Beatrica Ševčı́ková

Research Field

Correction: The dpsA Gene of Streptomyces coelicolor: Induction of Expression from a Single Promoter in Response to Environmental Stress or during Development

PLoS ONE (2011)

Paul D. Facey Beatrica Ševčı́ková Renáta Nováková Matthew D. Hitchings Jason C. Crack

The DpsA protein plays a dual role in Streptomyces coelicolor, both as part of the stress response and contributing to nucleoid condensation during sporulation.Promoter mapping experiments indicated that dpsA is transcribed from a single, sigB-like dependent promoter.Expression studies implicate SigH and SigB as the sigma factors responsible for dpsA expression while the contribution of other SigB-like factors is indirect by means of controlling sigH expression.The promoter is massively induced in response to osmotic stress, in part due to its sensitivity to changes in DNA supercoiling.In addition, we determined that WhiB is required for dpsA expression, particularly during development.Gel retardation experiments revealed direct interaction between apoWhiB and the dpsA promoter region, providing the first evidence for a direct WhiB target in S. coelicolor.

Environmental stress

10.1371/annotation/8fedd9bb-d84e-4d7b-bc71-3321e652198d

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Citations (4)

Phylogenetic analysis of therplA genes encoding ribosomal protein L1

Folia Microbiologica (2001)

Ľuboš Kľúčár Renáta Nováková Dagmar Homerová Beatrica Ševčı́ková Ján Turňa

Ribosomal protein

Streptomyces aureofaciens

28S ribosomal RNA

10.1007/bf02873585

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Citations (0)

Identification and transcriptional analysis of a cold shock-inducible gene, cspA, in Streptomyces coelicolor A3(2)

Molecular Genetics and Genomics (2000)

Ján Kormanec Beatrica Ševčı́ková

Identification

10.1007/s004380000298

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Citations (15)

A Method for Isolation of Small DNA Fragments from Agarose and Polyacrylamide Gels

Analytical Biochemistry (2001)

Ján Kormanec Dagmar Homerová Beatrica Ševčı́ková Bronislava Řežuchová

Agarose

Polyacrylamide

Isolation

10.1006/abio.2001.5048

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Citations (13)

An efficient blue-white screening system for markerless deletions and stable integrations in Streptomyces chromosomes based on the blue pigment indigoidine biosynthetic gene bpsA

Applied Microbiology and Biotechnology (2018)

Bronislava Řežuchová Dagmar Homerová Beatrica Ševčı́ková Luz Elena Núñez Renáta Nováková

Multiple cloning site

mCherry

Gene cluster

Heterologous

10.1007/s00253-018-9393-7

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Citations (18)

Strict control of auricin production in Streptomyces aureofaciens CCM 3239 involves a feedback mechanism

Applied Microbiology and Biotechnology (2012)

Peter Kutas Lubomira Feckova Alena Rehakova Renáta Nováková Dagmar Homerová

Streptomyces aureofaciens

Gene cluster

Streptomycetaceae

10.1007/s00253-012-4505-2

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Citations (19)

Complete Genome Sequence of Streptomyces lavendulae subsp. lavendulae CCM 3239 (Formerly “ Streptomyces aureofaciens CCM 3239”), a Producer of the Angucycline-Type Antibiotic Auricin

Genome Announcements (2018)

Tobias Busche Renáta Nováková Arwa Al-Dilaimi Dagmar Homerová Lubomira Feckova

ABSTRACT Streptomyces lavendulae subsp. lavendulae CCM 3239 produces the angucycline antibiotic auricin and was thought to be the type strain of Streptomyces aureofaciens . We report the complete genome sequence of this strain, which consists of a linear chromosome and the linear plasmid pSA3239, and demonstrate it to be S. lavendulae subsp. lavendulae .

Streptomyces aureofaciens

Strain (injury)

10.1128/genomea.00103-18

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Citations (12)

Activity of theStreptomyces coelicolorstress-response sigma factor Ï^His regulated by an anti-sigma factor

FEMS Microbiology Letters (2002)

Beatrica Ševčı́ková Ján Kormanec

The alternative sigma factor σH has been shown to play an important role in stress response and morphological differentiation in Streptomyces coelicolor. Its gene, sigH, is located in an operon with the gene encoding proposed anti-sigma factor UshX, and one of the promoters directing expression of the operon is dependent upon sigH. To clarify the function of S. coelicolor UshX, both the σH and UshX proteins were overproduced in Escherichia coli and purified. In an in vitro transcription assay, σH, after complementation with S. coelicolor core RNA polymerase, was able to recognize the sigH-dependent promoter, sigH-P2. This transcription was inhibited by UshX, if it was incubated with σH prior to the addition of the core RNA polymerase. When σH and UshX were incubated and electrophoresed through non-denaturing polyacrylamide gels, they formed a specific complex. These results showed that UshX is a specific anti-sigma factor for σH, and the S. coelicolor sigH operon is directly autoregulated.

Sigma factor

Transcription

Sigma

rpoS

10.1111/j.1574-6968.2002.tb11136.x

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Citations (22)

Activity of the Streptomyces coelicolor stress-response sigma factor σH is regulated by an anti-sigma factor

FEMS Microbiology Letters (2002)

Beatrica Ševčı́ková

Sigma factor

Sigma