Akio Fukusho

Nippon Veterinary and Life Science University

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Co-Authors

Hiroshi Aoki

Teikoku Seiyaku (Japan)

Yoshihiro Sakoda

Hokkaido University

Polly Roy

London School of Hygiene & Tropical Medicine

Kenichi Sakamoto

Icahn School of Medicine at Mount Sinai

Shigeyuki Nakamura

Ministry of Agriculture, Forestry and Fisheries

Masanobu Kamada

Japan Racing Association

Minoru Narita

Hoshi University

Shuichi Ueda

Nara Institute of Science and Technology

Y. Shimizu

Kobe University

N. Mizukoshi

National Institute of Animal Health

Cooperative Institutions

Hokkaido University

National Institute of Animal Health

The University of Tokyo

Nippon Veterinary and Life Science University

Ministry of Agriculture, Forestry and Fisheries

Kyoto University

Tokyo University of Agriculture and Technology

National Institute of Infectious Diseases

Suzuki (Japan)

Tokyo University of Science

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Characterization of Classical Swine Fever Virus Associated with Defective Interfering Particles Containing a Cytopathogenic Subgenomic RNA Isolated from Wild Boar.

Journal of Veterinary Medical Science (2001)

Hiroshi Aoki Kiyoyasu Ishikawa Yoshihiro Sakoda Hideto Sekiguchi Michi Kodama

Classical swine fever virus (CSFV) strain WB82, isolated from a wild boar in 1982, induced a distinct cytopathic effect (CPE) in primary swine testicle cell culture and in most of the porcine cell lines. This strain of CSFV was found to be composed of two biotypes, cytopathogenic (cp) CSFV, as a minor population, and noncytopathogenic (noncp) CSFV, as a major population. The noncp CSFV (designated strain WB82/E+) was obtained by biological cloning, and it showed the exaltation of Newcastle disease virus phenomenon. In Northern blot analysis and RT-PCR assay, CSFV RNA with a subgenomic (sg) length was detected in addition to full-length viral RNA only in the cells in which a CPE had been revealed. These RNAs represent the genomes of typical defective interfering (DI) particles because of the strict dependence on a complementing helper virus and interference with replication of the helper virus. The sg RNA, which exhibits the genomes of the DI particles, lacked the nucleotides of the viral genomic region from Npro to NS2 (4764 bases). When extracted sg RNA was transfected to the cells infected with the WB82/E+ strain, a distinct CPE was observed. Interestingly, the CPE was observed in cells infected with other heterologous noncp CSFV ALD and GPE- strains by sg RNA transfection. The results suggested that these noncp CSFVs act as helper viruses for the replication of sg RNA (DI particles). It was also shown that the cytopathogenicity of strain WB82 is caused by apoptosis.

Subgenomic mRNA

10.1292/jvms.63.751

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Citations (21)

Hemagglutination and hemagglutination-inhibition tests with porcine rotavirus.

PubMed (1987)

Yoshio EIGUCHI H Yamagishi Akio Fukusho Yohko K. Shimizu M. Matumoto

Hemagglutination assay

Hemagglutination tests

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Citations (1)

African Horsesickness Virus Infection in Adult Mice.

Journal of Equine Science (2000)

Kenichi Sakamoto Kenji Kawashima Minoru Narita Masanobu Kamada Akio Fukusho

Adult BALB/c mice were inoculated intranasally with African horsesickness virus serotype 4 (AHSV-4), the attenuated-vaccine strain. The 4-week-old mice died after developing nervous signs at 7 to 8 days post infection (p.i). Lesions, such as perivascular accumulation of lymphocytes and necrosis of neurons, were found only in the brains of virus-infected mice, but not in those of controls. Brains, red blood cells (RBC) and white blood cells (WBC) were collected from two mice each day p.i for virus isolation and RT-PCR. Virus could be isolated from brains collected at 2 to 7 days p.i of infection mice and only one of WBC sample collected on 7 day p.i, but no virus was isolated from any RBC samples. In contrast, RT-PCR products were visible in agarose gel electrophoresis stained with ethidium bromide from RBC and WBC samples collected at days 1 to 7 p.i, and from brain samples collected at days 2 to 7 p.i. The amplified PCR products were increased with days p.i when using extracted RNAs from brain samples, but the quantity of the PCR products was relatively constant with the RBC and WBC samples. It was shown that mice were susceptible to AHSV.

Ethidium bromide

Agarose gel electrophoresis

White blood cell

10.1294/jes.11.107

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Citations (1)

Coronavirus-, calicivirus-, and astrovirus-like particles associated with acute porcine gastroenteritis.

The Japanese Journal of Veterinary Science (1985)

Junsuke Shirai M. Shimizu Akio Fukusho

Diarrheal stools of an acute porcine gastroenteritis were examied by electron microscopy and inoculation into cell cultures. Two types of virus, coronavirus-like and calicivirus-like, were detected by direct electron microscopy, and cytopathogenic astrovirus-like virus was isolated in ESK cell cultures. This is the first report on the detection of calicivirus- and astrovirus-like viruses in porcine diarrhea in Japan, and on the isolation of cytopatogenic astrovirus-like virus in cell cultures.

Astrovirus

Calicivirus

Virus isolation

Coronavirus

Isolation

Feline calicivirus

10.1292/jvms1939.47.1023

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Citations (15)

END-phenomenon negative bovine viral diarrhea virus that induces the host's innate immune response supports propagation of BVDVs with different immunological properties

Virology (2019)

Mai Shiokawa Tsutomu Omatsu Yukie Katayama Kaoru Nishine Yuri Fujimoto

Pestivirus

10.1016/j.virol.2019.09.016

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Citations (6)

Variation from Cytopathogenic Biotype to Non-Cytopathogenic Biotype is Correlated with the Deletion of Cellular Sequence from Bovine Viral Diarrhea Viruses.

Journal of Veterinary Medical Science (1997)

Shigeyuki Nakamura Kenichi Sakamoto Yoshihiro Sakoda Tomoaki Shimazaki Yoshimitsu INOUE

Non-cytopathogenic (NCP) viruses of bovine viral diarrhea (BVD) virus were detected at a low ratio by the reverse plaque formation method from virus samples after several plaque clonings of cytopathogenic (CP) BVD viruses; NADL and Osloss strains. This phenomenon suggests that the NCP BVD viruses are produced at a low ratio during the propagation of CP BVD viruses in vitro. To investigate the differences between the parent CP BVD virus and the NCP BVD virus as a real progeny, the regions flanking the insertion of cellular mRNA in the p125 domain of NADL and Osloss strains were amplified by RT-PCR and cloned into pGEM 3Z plasmid vector, and then sequenced. Consequently, it was confirmed that sequences of cellular mRNA insertion of CP BVD viruses, NADL and Osloss strains, were completely and exactly deleted from the NCP BVD viruses which were real progeny of CP BVD viruses, NADL and Osloss strains. These results suggest that NCP BVD viruses may revert from CP biotype to NCP biotype by the deletion of cellular mRNA insertion in the viral genome of CP BVD viruses (NADL and Osloss strains).

Pestivirus

10.1292/jvms.59.361

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Citations (8)

Method for Detection of Extraneous Active Bovine Viral Diarrhoea Virus and Classical Swine Fever Virus in Animal Viral Vaccines by RT-PCR, Which Amplify Negative-strand Viral RNA in Infected Cells

Biologicals (2002)

Hiroshi Aoki Tomoaki Shimazaki Chikako Takahashi Yoshimasa Sasaki Shōko Suzuki

Classical swine fever

Primer (cosmetics)

Pseudorabies

Pestivirus

10.1006/biol.2001.0314

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Citations (5)

Electron microscopy of the intestine of gnotobiotic piglets infected with porcine rotavirus

Journal of Comparative Pathology (1982)

Minoru Narita Akio Fukusho Y. Shimizu

Lipid droplet

10.1016/0021-9975(82)90011-1

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Citations (7)

Field Distribution of END Phenomenon-Negative Bovine Viral Diarrhea Virus

Journal of Veterinary Medical Science (2014)

Kaoru Nishine Hiroshi Aoki Yoshihiro Sakoda Akio Fukusho

Field isolates of BVDV which do not show the exaltation of Newcastle disease virus (END) phenomenon (END–) are rarely reported. In this study, 45 BVDV field isolates from cattle in Hokkaido prefecture in Japan were analyzed by the reverse plaque formation method, the END method and observation of cytopathic effects. END– virus was detected in 34 of 45 isolates (75.6%), although 35 of 45 field isolates contained END phenomenon positive virus as the predominant virus population. We propose that END– viruses are widely distributed in the field and that it is possible that the mixture of biologically distinct BVDV correlates with the appearance of disease in infected animals.

Phenomenon

10.1292/jvms.14-0220

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Citations (4)

Synthesis of recombinant baculoviruses expressing the outer capsid protein VP2 of five BTV serotypes and the induction of neutralizing antibodies to homologous and heterologous BTV serotypes

Virus Research (1994)

T Urakawa T J French Yuchi Adachi Akio Fukusho H. Leblois

Heterologous

10.1016/0168-1702(94)90001-9

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Citations (16)