Rebecca J. Jepsen

National Animal Disease Center

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Lawrence E. Evans

Alpine Immune Sciences (United States)

Rich Stammer

Boehringer Ingelheim (United States)

Daniel W. Armstrong

The University of Texas at Arlington

Curtis R. Youngs

Iowa State University

Kyoung‐Jin Yoon

Iowa State University

Roman M. Pogranichniy

Kansas State University

Lingfeng He

National University of Malaysia

Steve Sorden

Northern Arizona University

Cooperative Institutions

Iowa State University

Kansas State University

Animal and Plant Health Inspection Service

Boehringer Ingelheim (United States)

The University of Texas at Arlington

University of Arkansas for Medical Sciences

Arizona Youth Partnership

Defence Science and Technology Laboratory

University of Oxford

Azyp (United States)

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Use of Direct Thaw Insemination to Establish Pregnancies with Frozen–Thawed Semen from a Standard Jack

Journal of Equine Veterinary Science (2010)

Rebecca J. Jepsen Lawrence E. Evans Curtis R. Youngs

Human chorionic gonadotropin

10.1016/j.jevs.2010.10.001

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Citations (12)

A novel approach to intrauterine viral inoculation of swine using PCV type 2 as a model

Theriogenology (2003)

Kyoung‐Jin Yoon Rebecca J. Jepsen Roman M. Pogranichniy Steve Sorden Rich Stammer

Transplacental

10.1016/j.theriogenology.2003.05.001

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Citations (31)

Electrophoretic Behavior and Potency Assessment of Boar Sperm Using a Capillary Electrophoresis−Laser Induced Fluorescence System

Analytical Chemistry (2003)

Lingfeng He Rebecca J. Jepsen Lawrence E. Evans Daniel W. Armstrong

The assessment of mammalian fertility, and the agents that affect it, is of increasing concern in medicinal, environmental, and agricultural science. The viability, integrity, and overall state of the male gamete (sperm) is an essential factor that must be considered in such studies. Traditional potency evaluations tend to be labor intensive and often are not precise. A CE-LIF technique for determining the viability of boar sperm was developed using the fluorescent stains SYBER-14 and propidium iodide. The buffer type, pH, ionic strength, applied voltage, and polymer additive must be optimized in order to obtain sharp peaks and accurate results. Extender solutions that are widely used in artificial insemination programs were found to be compatible and even beneficial for these CE-LIF experiments. A single viability assay takes less than 10 min, which is significantly faster than most other procedures. The compaction or focusing of the sample zone seems to be similar to that reported previously for microorganisms in CE.

BOAR

Propidium iodide

10.1021/ac026051r

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Citations (14)

The effect of hydroxypropyl-[beta]-cyclodextrin and egg yolk concentration on cryopreservation and post-thaw parameters of jack and stallion semen

Rebecca J. Jepsen

Pregnancy rates following artificial insemination with frozen-thawed jack spermatozoa have been relatively low compared to those attained in other species. Cholesterol is known to interfere with post-thaw fertility of jack and stallion semen. Altering the amount of cholesterol in the freezing extender may improve the fertility of frozen-thawed jack semen. Here we report clinical work completed with semen collected from a single jack; extended in EZ Mixin OF and slowly cooled to 5°C. Extended semen samples were then centrifuged at 400G for 10 minutes and the supernatant removed. The spermatozoa were frozen in liquid nitrogen vapor after resuspension in the appropriate freezing medium to a final concentration of 400 x 10 cells/mL. Freezing extender treatments were: 1) 20% Egg yolk (EY); 2) 5% EY; and 3) 20% EY + 60mM hydroxypropyl-β-cyclodextrin (β-CD). A total of 28 mares 2 to 18 years in age were utilized over 5 breeding seasons (82 total cycles). Mares were administered human chorionic gonadotropin (hCG) to induce ovulation when the dominant follicle was ≥ 35 mm as assessed by ultrasonography and were inseminated within 6 hours pre-ovulation and again within 6 hours post-ovulation. Pregnancy rates for each treatment were as follows: 1) 6.25% (1 pregnancy, 15 matings), 2) 46.5% (20 pregnancies, 43 matings), 3) 58.5% (14 pregnancies, 24 matings). These data support the theory of cholesterol interfering with post-thaw fertility of jack semen. We have established that mule pregnancies can be achieved at acceptable rates with frozen-thawed jack semen cryopreserved in 5%EY and 20% EY + 60 mM β-cyclodextrin transferred directly post-thaw. Cyclodextrins are reported to improve post-thaw viability and motility in boar semen. Cyclodextrin mediates cholesterol efflux and subsequent acrosome reaction and capacitation in the sperm cells of several species in vitro. The second objective of this study was to

Semen cryopreservation

Human chorionic gonadotropin

10.31274/rtd-180813-17176

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