Cong Liang Deng

Chinese Academy of Inspection and Quarantine

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Tao Song

Institute of Electrical Engineering

Yong Jie Dou

Chinese Academy of Sciences

Jian Xu

Harbin Engineering University

Leng Nie

Chinese Academy of Sciences

Yang Yu

Chinese Academy of Sciences

Xiaoli Zhao

Tongji University

Ming Wang

Northeast Normal University

Ming Wang

Anhui Agricultural University

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Chinese Academy of Sciences

University of Chinese Academy of Sciences

Institute of Electrical Engineering

Ministry of Ecology and Environment

Beijing National Laboratory for Molecular Sciences

Shanghai Jiao Tong University

Tongji University

Chongqing University of Technology

Zhejiang University

Anhui Water Conservancy and Hydropower Survey and Design Institute

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Automated Extraction of Plant Viral RNA Using the Nucleic Acid Instrument Based on Magnetic Nanobeads

Applied Mechanics and Materials (2013)

Yong Jie Dou Xiaoli Zhao Jian Xu Cong Liang Deng Leng Nie

In this paper, an automated nucleic acid extraction instrument based on magnetic nanobeads is described. Also, a prototype is designed and constructed. Some plant viral RNA extraction experiments were done using the platform. And the experimental results demonstrated that the instrument could be successfully used for extraction of plant viral RNA，and the extracted RNA was successfully used in an automated PCR assay for the detection of plant virus. The Ct values of CGMMV RNA, LSV RNA and ArMV RNA at the initial concentration were 12.51, 17.81 and 23.48.

10.4028/www.scientific.net/amm.303-306.646

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Design of Automatic Nucleic Acid Extraction Instrument Based on Magnetic Nanobeads

Advanced materials research (2012)

Yong Jie Dou Jian Xu Cong Liang Deng Xiaoli Zhao Leng Nie

In this paper, an automatic nucleic acid extraction instrument is described. It consists of mechanical components, motor controlling module, temperature controlling module and computer. Also, a prototype has been designed and constructed. The maximum sample throughput of this prototype is 96, the sample volume is 300-1000ul, the maximum sample handling time is about 60min, the sample temperature controlling range is from room temperature to 95oC, and the accuracy of temperature controlling achieved is about ± 2oC.

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Atmospheric temperature range

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10.4028/www.scientific.net/amr.621.313

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