The objective of this study was to investigate the impact of male age, semen quality and days of ejaculatory abstinence on embryo morphokinetics. A total of 1,220 zygotes obtained from 139 couples in a private in vitro fertilisation centre were analysed. The timing of specific events from the point of insemination, such as timings to pronuclei appearance and fading, to two, three, four, five, six, seven and eight cells and to blastulation were recorded. Multivariate linear regression analysis was used to evaluate the influence of paternal factors on embryo morphokinetic events. Paternal age was positively correlated with delayed cell cleavage and blastulation, and negatively associated with implantation rate, and clinical pregnancy and live-birth chances. The ejaculatory abstinence was inversely correlated with the implantation rate. Inverse relationships were observed between semen parameters (sperm count, progressive sperm motility, total motile sperm count and morphology) and the timing of specific events during embryo development. Sperm morphology was also positively associated with implantation rate and pregnancy and live-birth chances. Increased paternal age and ejaculatory abstinence, and poor semen quality correlate with delayed cell cleavage and blastulation and negatively impact intracytoplasmic sperm injection outcomes.

Pronucleus

Blastula

Semen quality

10.1111/and.14211

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Citations (5)

Endometriosis Affects Oocyte Morphology in Intracytoplasmic Sperm Injection Cycles

JBRA (2015)

Edson Borges D. P. A. F. Braga Amanda Souza Setti Livia Vingris R. C. S. Figueira

To identify associations between presence of endometriosis and oocyte defects, embryo developmental potential, and cycle outcomes.This study looked into the impact of endometriosis on oocyte and embryo quality, and blastocyst formation probability. Endometriosis was also correlated with cycle characteristics. In order to avoid age-related bias, in the first analysis only patients aged 36 years or younger were included, and the cycles were split into endometriosis infertility cycles (n=431; 3172 oocytes) and other cycles (n=2510; 24480 oocytes).The number of retrieved oocytes (10.6±21.2 vs. 14.6±21.1, P<0.001), oocyte yield (68.1±20.0% vs. 70.6±19.6%, P=0.015), and embryos obtained (6.1±4.43 vs. 7.8±5.12, P<0.001) were lower among patients with endometriosis. Implantation rates (28.1%±38.9% vs. 33.9±42.7, P<0.001) were lower among patients with endometriosis, but fertilization, pregnancy, miscarriage and cycle cancelation rates were not different. There was a significant increase in the incidence of extra-cytoplasmic, but not intra-cytoplasmic, oocyte defects among patients with endometriosis. The quality of embryos (45.3% vs. 47.3%, P=0.037) collected from patients with endometriosis was lower, but blastocyst formation rates were unaltered.A possible explanation for the lower implantation rates seen in patients with endometriosis is the poorer quality of the oocytes and embryos observed in this group of patients.

Embryo quality

Oocyte activation

10.5935/1518-0557.20150046

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Citations (29)

SESSION 33: MALE INFERTILITY - CLINICAL AND LABORATORY ASPECTS

Human Reproduction (2012)

Jin-Ku Byun Chong Won Bak J. H. Lee Y. J. Ju S. Han

10.1093/humrep/27.s2.32

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Parameters for embryo developmentin vitro: The role of the WHO guidelines (2010) in the characterization of abnormal sperm

JBRA (2013)

Edson Borges Amanda Souza Setti Daniela Paes de Almeida Ferreira Braga Rita de Cassia Sávio Figueira Livia Vingris

Introduction:The role of paternal factors in embryonic development remains controversial.Moreover, it is unclear whether the new reference values for semen parameters correlate with ICSI outcomes.The objective of this study was to determine whether the assessment of sperm parameters according to the new reference values is associated with the frequency of high-quality embryos and ICSI cycle outcomes.Materials and Methods: A total of 385 cycles was inclu-(ASP group, n=175) and normal sperm parameter group (NSP group, n=210).The ICSI outcomes were then compared between the groups.Regression analysis models were on high-quality embryos and the ICSI outcomes.Results high-quality embryo rate (RC: 0.139, p = 0.042). Conclusion: This study demonstrated that sperm progressi-recently established reference values for semen characteristics are of limited value in predicting embryonic development.

10.5935/1518-0557.20130064

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The male factor of infertility should not be an issue for the selection of patients for extended embryo culture programs

Fertility and Sterility (2013)

E. Borges D. P. A. F. Braga Livia Vingris R. C. S. Figueira Amanda Souza Setti

10.1016/j.fertnstert.2013.07.529

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The prevalence of sperm with large nuclear vacuoles is a prognostic tool in the prediction of ICSI success

Journal of Assisted Reproduction and Genetics (2013)

Amanda Souza Setti Daniela Paes de Almeida Ferreira Braga Livia Vingris Rita de Cassia Sávio Figueira Assumpto Iaconelli

Semen Analysis

10.1007/s10815-013-0157-0

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Citations (9)