Guohua Xu

Nanjing Agricultural University

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Co-Authors

Xiaorong Fan

Nanjing Agricultural University

Mian Gu

Nanjing Agricultural University

Qirong Shen

Nanjing Agricultural University

Aiqun Chen

Nanjing Agricultural University

Shubin Sun

Nanjing Agricultural University

Yali Zhang

Nanjing Agricultural University

Hongye Qu

Nanjing Agricultural University

Ling Yu

University of Florida

Huimin Feng

Nanchang Institute of Technology

Shaoling Zhang

Nanjing Agricultural University

Cooperative Institutions

Nanjing Agricultural University

420

Chinese Academy of Sciences

188

Ministry of Agriculture and Rural Affairs

137

Zhejiang University

131

Chinese Academy of Agricultural Sciences

University of Chinese Academy of Sciences

Sun Yat-sen University

Shanghai Jiao Tong University

Sichuan University

Peking University

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OsNLP3 and OsPHR2 orchestrate direct and mycorrhizal pathways for nitrate uptake by regulating NAR2.1–NRT2s complexes in rice

Proceedings of the National Academy of Sciences (2025)

Shuangshuang Wang Hanghang Ye Congfan Yang Yan Zhang Jia Pu

Nitrogen (N) is the most important essential nutrient required by plants. Most land plants have evolved two N uptake pathways, a direct root pathway and a symbiotic pathway, via association with arbuscular mycorrhizal (AM) fungi. However, the interaction between the two pathways is ambiguous. Here, we report that OsNAR2.1–OsNRT2s, the nitrate (NO 3 − ) transporter complexes with crucial roles in direct NO 3 − uptake, are also recruited for symbiotic NO 3 − uptake. OsNAR2.1 and OsNRT2.1/2.2 are coregulated by NIN-like protein 3 (OsNLP3), a key regulator in NO 3 − signaling, and OsPHR2, a major regulator of phosphate starvation responses. More importantly, AM symbiosis induces expression of OsNAR2.1 – OsNRT2s, OsNLP3, and OsSPX4 , encoding an intracellular Pi sensor, in arbuscular-containing cells, but weakens their expression in the epidermis. OsNAR2.1 and OsNLP3 can activate both mycorrhizal NO 3 − uptake and mycorrhization efficiency. Overall, we demonstrate that OsNLP3 and OsPHR2 orchestrate the direct and mycorrhizal NO 3 − uptake pathways by regulating the NAR2.1–NRT2s complexes in rice.

Arbuscular mycorrhiza

10.1073/pnas.2416345122

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Transcription factor OsSHR2 regulates rice architecture and yield per plant in response to nitrogen

Planta (2024)

Zhi Hu Xu Huang Huihuang Xia Zhantian Zhang Huixin Lu

10.1007/s00425-024-04400-7

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Advances in the Uptake and Transport Mechanisms and QTLs Mapping of Cadmium in Rice

International Journal of Molecular Sciences (2019)

Jingguang Chen Wenli Zou Lijun Meng Xiaorong Fan Guohua Xu

Cadmium (Cd), as a heavy metal, presents substantial biological toxicity and has harmful effects on human health. To lower the ingress levels of human Cd, it is necessary for Cd content in food crops to be reduced, which is of considerable significance for ensuring food safety. This review will summarize the genetic traits of Cd accumulation in rice and examine the mechanism of Cd uptake and translocation in rice. The status of genes related to Cd stress and Cd accumulation in rice in recent years will be summarized, and the genes related to Cd accumulation in rice will be classified according to their functions. In addition, an overview of quantitative trait loci (QTLs) mapping populations in rice will be introduced, aiming to provide a theoretical reference for the breeding of rice varieties with low Cd accumulation. Finally, existing problems and prospects will be put forward.

10.3390/ijms20143417

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Functional characterization of OsHAK1 promoter in response to osmotic/drought stress by deletion analysis in transgenic rice

Plant Growth Regulation (2019)

Guang Chen Jiang Hu Juan Lian Yu Zhang Li Zhu

Osmotic shock

CAAT box

TATA box

Genetically modified rice

10.1007/s10725-019-00504-3

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Variation in the Abundance of OsHAK1 Transcript Underlies the Differential Salinity Tolerance of an indica and a japonica Rice Cultivar

Frontiers in Plant Science (2018)

Guang Chen Chaolei Liu Zhenyu Gao Yu Zhang Anpeng Zhang

Salinity imposes a major constraint over the productivity of rice. A set of chromosome segment substitution lines (CSSLs), derived from a cross between the japonica type cultivar (cv.) Nipponbare (salinity sensitive) and the indica type cv. 9311 (moderately tolerant), was scored using a hydroponics system for their salinity tolerance at the seedling stage. Two of the CSSLs, which share a ∼1.2 Mbp stretch of chromosome 4 derived from cv. Nipponbare, were as sensitive to the stress as cv. Nipponbare itself. Fine mapping based on an F2 population bred from a backcross between one of these CSSLs and cv. 9311 narrowed this region to 95 Kbp, within which only one gene (OsHAK1) exhibited a differential (lower) transcript abundance in cv. Nipponbare and the two CSSLs compared to in cv. 9311. The gene was up-regulated by exposure to salinity stress both in the root and the shoot, while a knockout mutant proved to be more salinity sensitive than its wild type with respect to its growth at both the vegetative and reproductive stages. Seedlings over-expressing OsHAK1 were more tolerant than wild type, displaying a superior photosynthetic rate, a higher leaf chlorophyll content, an enhanced accumulation of proline and a reduced level of lipid peroxidation. At the transcriptome level, the over-expression of OsHAK1 stimulated a number of stress-responsive genes as well as four genes known to be involved in Na+ homeostasis and the salinity response (OsHKT1;5, OsSOS1, OsLti6a and OsLti6b). When the stress was applied at booting through to maturity, the OsHAK1 over-expressors out-yielded wild type by 25%, and no negative pleiotropic effects were expressed in plants gown under non-saline conditions. The level of expression of OsHAK1 was correlated with Na+/K+ homeostasis, which implies that the gene should be explored a target for molecular approaches to the improvement of salinity tolerance in rice.

10.3389/fpls.2017.02216

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Effect of hepatocyte growth factor on expression of connective tissue growth factor in cardiac fibroblasts

Practical Journal of Medicine & Pharmacy (2007)

Guohua Xu

Objective To observe the effect of hepatocyte growth factor on the expression of connective tissue growth factor (CTGF) in cultured cardiac fibroblast(CFs).Methods CFs of neonatal Sprague-Dawley (SD)rats were isolated and cultured. Immunocytochemistry staining was used to monitor the marker antigen of the neonatal rat cardiac fibroblasts. HGF 100ng/ml and AngⅡof 10-6mol/L were added to the medium for 48 hours to observe the effects of HGF on CTGF mRNA and protein expression stimulated by AngⅡ.The expression of CTGF mRNA and protein were detected by reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot respectively.Results HGF could partly reduce the AngⅡ-dependent expression of CTGF both in mRNA and protein level. Conclusion HGF and CTGF may be a couple of contradictory growth factor. HGF could delay the procession of their myocardial fibrosis partly because of inhibiting the expression of CTGF.

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Research on the Technology of Deep Bed Filtration Used in Wastewater Treatment from Waste Plastics Granulation

Guohua Xu

Waste plastics granulation process used a large amount of water to clean surface remainders,and there were much SS in wastewater.The technology of coagulation and deep bed filtration was used.After treatment,the outlet can reach the water quality standard of domestic miscellaneous water(GB/T18920-2002).This process was characterized by low dosage,small area.

Granulation

Filtration (mathematics)

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Design, synthesis and antitubulin activity of novel podophyllotoxin derivatives as potent anticancer agent

Chemical Research in Chinese Universities (2015)

Lifei Bai Renlei Wang Ye Zou Guohua Xu

Colchicine

10.1007/s40242-015-5325-6

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The Role of a Potassium Transporter OsHAK5 in Potassium Acquisition and Transport from Roots to Shoots in Rice at Low Potassium Supply Levels

PLANT PHYSIOLOGY (2014)

Tianyuan Yang Song Zhang Yibing Hu Fachi Wu Qingdi Hu

In plants, K transporter (KT)/high affinity K transporter (HAK)/K uptake permease (KUP) is the largest potassium (K) transporter family; however, few of the members have had their physiological functions characterized in planta. Here, we studied OsHAK5 of the KT/HAK/KUP family in rice (Oryza sativa). We determined its cellular and tissue localization and analyzed its functions in rice using both OsHAK5 knockout mutants and overexpression lines in three genetic backgrounds. A β-glucuronidase reporter driven by the OsHAK5 native promoter indicated OsHAK5 expression in various tissue organs from root to seed, abundantly in root epidermis and stele, the vascular tissues, and mesophyll cells. Net K influx rate in roots and K transport from roots to aerial parts were severely impaired by OsHAK5 knockout but increased by OsHAK5 overexpression in 0.1 and 0.3 mm K external solution. The contribution of OsHAK5 to K mobilization within the rice plant was confirmed further by the change of K concentration in the xylem sap and K distribution in the transgenic lines when K was removed completely from the external solution. Overexpression of OsHAK5 increased the K-sodium concentration ratio in the shoots and salt stress tolerance (shoot growth), while knockout of OsHAK5 decreased the K-sodium concentration ratio in the shoots, resulting in sensitivity to salt stress. Taken together, these results demonstrate that OsHAK5 plays a major role in K acquisition by roots faced with low external K and in K upward transport from roots to shoots in K-deficient rice plants.

Rice plant

Potassium deficiency

10.1104/pp.114.246520

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Citations (309)

OsNRT2.4 encodes a dual-affinity nitrate transporter and functions in nitrate-regulated root growth and nitrate distribution in rice

Journal of Experimental Botany (2018)

Jia Wang Zheng Yi Huimin Feng Hongye Qu Xiaorong Fan

Plant NRT2 nitrate transporters commonly require a partner protein, NAR2, for transporting nitrate at low concentrations, but their role in plants is not well understood. In this study, we characterized the gene for one of these transporters in the rice genome, OsNRT2.4, in terms of its activity and roles in rice grown in environments with different N supply. In Xenopus oocytes, OsNRT2.4 alone without OsNAR2 co-expression facilitated nitrate uptake showing biphasic kinetics at a wide concentration range, with high- and low-affinity KM values of 0.15 and 4 mM, respectively. OsNRT2.4 did not have nitrate efflux or IAA influx activity. In rice roots, OsNRT2.4 was expressed mainly in the base of lateral root primordia. Knockout of OsNRT2.4 decreased lateral root number and length, and the total N uptake per plant at both 0.25 and 2.5 mM NO3− levels. In the shoots, OsNRT2.4 was expressed mainly in vascular tissues, and its knockout decreased the growth and NO3−-N distribution. Knockout of OsNRT2.4, however, did not affect rice growth and N uptake under conditions without N or with only NH4+ supply. We conclude that OsNRT2.4 functions as a dual-affinity nitrate transporter and is required for nitrate-regulated root and shoot growth of rice.

Primordium

Efflux

Lateral root

10.1093/jxb/erx486

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Citations (88)