The C 3 polymorphism was studied by agarose gel electrophoresis in a series of serum samples from 148 Swedish Lapps. The frequencies of the genes C31 and C32 were estimated to be 0.027 and 0.973, respectively. The distribution of C 3 phenotypes in Swedish Lapps was significantly different from that among Swedes. Reexaminations of certain sera indicated that the previously described phenotype 2–4 may be a storage artefact.
In a controlled study of fibronectin supplementation in sepsis, 11 ICU patients in septic shock were scheduled to receive either cryoprecipitate from 20-40 donors (n = 6) or 250-300 ml of stored plasma (n = 5) (two infusions over 24 h). We wanted to: compare some "conventional" DIC variables in the ICU (platelet count, prothrombin complex = NT, FDP) to additional variables: Fibronectin (Fn), fibrinogen (Fg), F V, FVIII R:Ag, F VIII:C activity, F XII, plasminogen (Plg), antiplasmin (AP), antithrombin (AT), kallikrein inhibiting activity (KI) and spontaneous proteolytic activity (SPA): study the effects of cryoprecipitate or plasma infusion on three variables. Samples were taken before the first infusion, and 24 and 48 h after. At onset, high levels (p less than .001 when compared to blood donors) of Fg, VIIIR:Ag and VIII:C were seen. KI levels were within the normal range. F V was low (p less than .05). Fn, NT, XII, Plg, AP and AT were markedly low (p less than .001). SPA showed great variation. When compared to 28 patients with severe infections, but not in septic shock, the ICU group had higher VIIIR:Ag (p less than .05) and VIII:C (p less than .01), and lower XII, Plg, AP and AT (p less than .001). FDP was elevated in all ICU patients. Five patients were thrombocytopenic, and in these a pattern with low levels of Plg and AT was observed. Fn did not correlate well to the other variables measured. These results indicate a marked activation of coagulation and fibrinolysis in these severely ill patients.(ABSTRACT TRUNCATED AT 250 WORDS)
Blood was drawn after the intranasal administration of 325 μg DDAVP from altogether 420 blood donors at 5 blood banks in Sweden. No significant side‐effects were observed. The DDAVP plasma was pooled in two separate batches. Matched pools of control plasma from the same donors were also prepared. From each pool a separate batch of high purity factor VIII concentrate (Octonativ) was produced. The yield of VIII:c in the DDAVP batches was 2.6 and 1.5 times higher than in the corresponding control batches. The specific activity was 2.8 for the two DDAVP batches and 2.0 IU/mg protein for the control batches. The in vivo recovery was about 100 per cent for all the batches when examined in 5 patients with severe haemophilia a. The individual half‐lives (late phase 5–48 hours) varied between 7 and 17 hours. No significant differences between the batches derived from the DDAVP and the control plasma could be detected. Thus, intranasal DDAVP seems to be a safe and practicable means of improving the production of factor vIII concentrates.
Intensive plasmapheresis was performed on 7 alloimmunized pregnant women. About 60% of the plasma volume was removed each day, Monday to Friday, with pauses over the weekends. This treatment resulted in drops in immunoglobulin levels to about 1/3 of the pre‐treatment concentrations, whereas the haemostatic parameters studied remained within normal limits. Thus, generally the treatment (as performed in this study) does not seem to induce changes that predispose for thrombotic or haemorrhagic complications. However, in one of the patients, antithrombin III became progressively depleted over the weeks of treatment. Therefore it is suggested that the patients be screened for their haemostatic profile prior to and after a few weeks of plasmapheresis treatment.
HLA (A and B) antigens, blood group systems (ABO, Rh, MNSs, P, Kell, Lewis and Duffy) and serum group systems (Hp, Tf, Pi, C3 and C4) were studied in patients with intermittent claudication (IC) and controls. HLA antigen A 28 was significantly more common, and blood group 0 was significantly less common among the patients than among the controls. A comparison between patients with IC and those with abdominal aortic aneurysms showed a significant difference between these two groups concerning the MN blood groups.
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