Hussein Sabit

Misr University for Science and Technology

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Shaimaa Abdel-Ghany

Misr University for Science and Technology

Hüseyin Tombuloğlu

Imam Abdulrahman Bin Faisal University

EÇ

Emre Çevik

Imam Abdulrahman Bin Faisal University

Osama A. M. Said

Misr University for Science and Technology

Amany I. Alqosaibi

Imam Abdulrahman Bin Faisal University

Emre Cevik

Imam Abdulrahman Bin Faisal University

Mohamed A. Abdel-Hakeem

Misr University for Science and Technology

Ayhan Bozkurt

Imam Abdulrahman Bin Faisal University

Suriya Rehman

Imam Abdulrahman Bin Faisal University

Thamer Alshammari

Florida Atlantic University

Cooperative Institutions

University of Washington

138

University of Nebraska Medical Center

124

University of California, Irvine Medical Center

120

Seattle Cancer Care Alliance

119

Sheffield Teaching Hospitals NHS Foundation Trust

117

Fred Hutch Cancer Center

115

Levine Cancer Institute

101

Imam Abdulrahman Bin Faisal University

The University of Texas MD Anderson Cancer Center

Misr University for Science and Technology

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A Wild Fomes fomentarius for Biomediation of One Pot Synthesis of Titanium Oxide and Silver Nanoparticles for Antibacterial and Anticancer Application

Biomolecules (2020)

Suriya Rehman Romana Farooq B. Rabindran Jermy Sarah Mousa Asiri Vijaya Ravinayagam

The present study offers an alternative method for green synthesis of the formation of two types of nanoparticles (NPs). These NPs, titanium oxide and silver NPs (TiO2 and Ag NPs, respectively), were obtained from the amalgamation of intracellular extract of a wild mushroom, Fomes fomentarius, with aqueous solutions of titanium isopropoxide and silver nitrate, respectively. F. fomentarius was identified phenotypically and by 18S ribosomal RNA gene sequencing (Gene accession no: MK635351). The biosynthesis of TiO2 and Ag NPs was studied and characterized by X-ray diffraction (XRD), diffuse reflectance UV-Visible spectroscopy (DR-UV), fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and transmission electron microscope (TEM). Success was achieved in obtaining NPs of differing sizes and shapes. The antibacterial and anticancer activity of the NPs was significant with morphological damage being caused by both, although Ag NPs (10-20 nm) were found to have profound effects on bacterial and cancer cells in comparison to TiO2 NPs (100-120 nm). These metal NPs, synthesized using wild mushrooms, hold a great potential in biomedicinedue to an effective enzyme combination, which permits them to modify different chemical compounds to less toxic forms, which is required for ecofriendly and safe biomaterials.

Silver nanoparticle

Silver nitrate

10.3390/biom10040622

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Citations (52)

Impact of Ultraviolet Radiation on the Genetic Background of Egyptian Freshwater Leech; Barbronia assiuti

Nasser A. El-Shimy Z. El-Bakry Hussein Sabit

3 ABSTRACT: Ultraviolet (UV), one of the components of solar radiation, is divided into UV-A (320-400 nm), UV-B (280-320•nm) and UV-C (200-280 nm). Under normal conditions, many vertebrates and invertebrates can protect themselves from UV by skin pigments or integuments, such as feathers, hairs or scales. Leeches are common in a wide range of fresh water habitat in Egypt. This makes them one of the most suitable bio-indicator organisms for testing UV-A. The present study aimed at elucidating the negative impact of UV-A on the histology and histopathology of the body wall, epidermis, muscles, pigments, botryoidal tissue and reproductive organs. Molecular genetic analysis was performed in order to assess the effects of UV-A on the genetic background of Barbronia assiuti.

Ultraviolet

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The Long-Term Impact of COVID-19 Pneumonia on the Pulmonary Function of Survivors

International Journal of General Medicine (2021)

Ayad Mohammed Salem Noor Al Khathlan Abdulelah Fawzi Alharbi Turki Alghamdi Saleh AlDuilej

The sequelae of COVID-19 pneumonia on pulmonary function and airways inflammation are still an area of active research.This research aimed to explore the long-term impact of COVID-19 pneumonia on the lung function after three months from recovery.Fifty subjects (age 18-60 years) were recruited and classified into two groups: the control group (30 subjects) and the post-COVID-19 pneumonia group (20 patients). Pulmonary function tests, spirometry, body plethysmography [lung volumes and airway resistance (Raw)], diffusion capacity for carbon monoxide (DLCO), and fractional exhaled nitric oxide (FeNO), were measured after at least 3 months post-recovery.Significant reduction in total lung capacity (TLC), forced vital capacity (FVC), forced expiratory volume (FEV1), FEV1/FEV, and diffusing capacity for carbon monoxide (DLCO) was observed in post-COVID-19 subjects compared to controls. Restrictive lung impairment was observed in 50% of post-COVID-19 cases (n = 10) compared to 20% in the control group (n = 6, P = 0.026). In addition, mild diffusion defect was detected in 35% (n = 7) of the post-COVID-19 group compared to 23.3% (n = 7) in the controls (P = 0.012).COVID-19 pneumonia has an impact on the lung functions in terms of restrictive lung impairment and mild diffusion defect after three months from recovery. Therefore, a long-term follow-up of the lung function in post-COVID-19 survivors is recommended.

DLCO

Vital capacity

10.2147/ijgm.s319436

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Citations (61)

Effect of Nigella sativa on general health and immune system in young healthy volunteers; a randomized, placebo-controlled, double-blinded clinical trial

F1000Research (2023)

Ayad Mohammed Salem Abdullah Bamosa Mohammed H Alam Saleh Alshuraim Hamad Alyalak

Nigella sativa (N. sativa) is traditionally used as an immune enhancer in different communities. The aim of this study was to evaluate the effect of N. sativa on immunity related parameters in young healthy subjects. This study was a double blind, randomized, placebo controlled clinical trial. Fifty-two healthy subjects (48 male and 4 female) 18-25 years old were enrolled in the study. They were randomly divided into four groups; the first received charcoal capsules and served as controls and the other three received 0.5, 1 g, and 2 g of powdered N. sativa capsules, respectively. Two blood samples were obtained from all participant, before initiation of the trial and at the end of the four weeks intervention. One sample was used for routine health screening by evaluating liver and renal functions as well as complete blood count and differential. The second sample was used to measure certain cytokines including; IL-1, IL-4, IL-6, IL-10, and TNF. A third and fourth samples were obtained from the last cohort of subjects before and after treatment; the third was used for measuring immunoglobulins and CD profile and the fourth for evaluating certain gene expressions (INF-γ, NF-κ-B, TNF-α, IL-1β, IL-13, IL-8, and IL-6). Only 1 g dose of N. sativa produced a significant elevation in total lymphocyte count, CD3+ and CD4+ counts. One gram N. sativa increased the absolute lymphocyte count from 1850±0.24 to 2170±0.26 (p=0.008), CD3+ from 1184.4±75.60 to 1424±114.51 (p=0.009), and CD4+ from 665.6±141.66 to 841±143.36 (p=0.002). This elevation in T cells was lost by increasing the dose of N. sativa to 2g. The rest of the parameters were not changed significantly in all doses. The results show a promising immunopotentiation effect of N. sativa by elevating helper T cells and the optimum dose for young age group seems to be 1 g.

Nigella sativa

Asparagus racemosus

10.12688/f1000research.73524.2

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Citations (3)

Peer Review #2 of "DisCoVering potential candidates of RNAi-based therapy for COVID-19 using computational methods (v0.1)"

Hussein Sabit

The ongoing pandemic of a novel coronavirus (SARS-CoV-2) leads to international concern; thus, emergency interventions need to be taken.Due to the time-consuming experimental methods for proposing useful treatment, computational approaches facilitate investigating thousands of alternatives simultaneously and narrow down the cases for experimental validation.Herein, we conducted four independent analyses on RNA interference (RNAi)based therapy with computational and bioinformatic methods.The aim is to target the evolutionarily conserved regions in the SARS-CoV-2 genome in order to down-regulate or silence its RNA.miRNAs are denoted to play an important role in the resistance of some species to viral infections.A comprehensive analysis of the miRNAs available in the body of humans, as well as the miRNAs in bats and many other species, were done to find efficient candidates with low side effects in the human body.Moreover, the evolutionarily conserved regions in the SARS-CoV-2 genome were considered for designing novel significant siRNA that are target-specific.A small set of miRNAs and two siRNA were suggested as the possible efficient candidates with a high affinity to the SARS-CoV-2 genome and low side effects.The suggested candidates are promising therapeutics for the experimental evaluations and may speed up the procedure of treatment design.

10.7287/peerj.10505v0.1/reviews/2

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Vorinostat enhances the therapeutic potential of Erlotinib via MAPK in lung cancer cells

Cancer Treatment and Research Communications (2022)

Amany I. Alqosaibi Shaimaa Abdel-Ghany Fatma A. Al-Mulhim Hussein Sabit

Lung cancer is the second most common cancer in both men and women, with an estimated 235,760 new cases and 131,880 deaths in 2021 in the US. Despite the modern therapies being available such as radiotherapy and chemotherapy, death rates are still increasing. Erlotinib (ERL) is one of the treatment options for lung cancer, although the probability for the patients to develop resistance to ERL constrains its reliability. The aim of the present study is to assess the synergetic effect of combining ERL with vorinostat (SAHA) on the progression of lung cancer cells.Adenocarcinoma alveolar basal epithelial cells (A549) were treated with either ERL, SAHA as mono drugs or with the combination of them for 24 h. Cytotoxicity assay and cell cycle analysis along with apoptosis detection were investigated. The expression profile of CDH1, TGF1, and MAPK was also assessed. Results showed an elevation in the apoptosis level in all treatments compared to WISH; the normal human amnion-derived cells. Furthermore, the treatments caused the cell cycle to arrest at G2/M, indicating its cytotoxic activity.The combination of SAHA and ERL significantly increased the level of apoptosis in lung cancer cells. Meanwhile, this combination treatment downregulated MAPK compared to the mono drugs and the control cells, suggesting the potential role of MAPK in regulating apoptosis and cell cycle machinery in lung cancer.

Vorinostat

10.1016/j.ctarc.2022.100509

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Citations (12)

Small Interference RNA-Mediated Down Regulation of Survivin Expression in Breast and Hepatocellular Carcinoma Cell Lines

Hussein Sabit Hala B. Al-Sharkawy Mariam Al-Zaidy Mohamed A. Abdel-Baseer Nabil S. Awad

4 Abstract: Survivin, a member of inhibitor of apoptosis protein family, has become an attractive therapeutic target in cancer due to its selective expression in different malignant tumors. It has a dual function in apoptosis suppression and in cell cycle progression. In the present study, we evaluated the role of Survivin knockdown in reactivating the apoptotic machinery and hence in reducing cell proliferation. Lipofection-based approach was used to deliver small interfering RNAs (siRNAs) to down regulate Survivin expression in two different cancerous cell line (MCF-7 for breast cancer and HebG2 for hepatocellular carcinoma). The obtained results showed that down regulation of Survivin has affected the overall cell proliferation via activating caspases- mediated apoptosis. 3-(4, 5-dimethylthylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay was employed to assess the viability of the transfected cells. Cell toxicity was evaluated by Trypan blue test. Real- Time PCR was conducted to assess the gene expression of the cells after being transfected. The results demonstrated that Survivin mRNA translation was inhibited and this has led to attenuation of the malignant features of breast and hepatocellular carcinoma cell lines. On the light of our conclusion, Survivin-targeting siRNA may provide a novel approach to control cancer due to its specificity.

Survivin

Viability assay

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Citations (2)

Alginate-guided size and morphology-controlled synthesis of MnO₂nanoflakes

Soft Materials (2019)

Sarah Mousa Asiri Emre Çevik Hussein Sabit Ayhan Bozkurt

This work reports the morphology, crystal size, and shape variations of nanoparticles, including manganese dioxide- MnO2 during in situ synthesis in biopolymer. In this context, stable nanocomposite based on sodium alginate (Alg) – MnO2 were produced in different solutions by changing the reaction conditions. Then the host polymer, Alg was removed via calcination to obtain neat nanoflakes. The chemical, thermal, morphology, and structural properties as well as porosity of pure MnO2, Alg-MnO2, and calcinated products were investigated using scanning electron microscopy (SEM), Transmission electron microscopy (TEM), X‐ray diffraction (XRD), thermogravimetric analysis (TGA), differential thermal analysis (DTA), Brunauer-Emmett-Teller (BET), and Fourier transform infrared spectroscopy (FT-IR). It was clearly demonstrated that the presence different solvents, polymer, and the pH variations affected noticeably on the crystal structures of the samples. Additionally, nanorods monodispersed particles with length and width around 200 and 7–10 nm were produced without Alg in isopropanol. Whereas, in the same condition, the morphology of MnO2 changed to nanoflakes structures in the existence of Alg and size diminished to quarter (50 nm) of the pristine material. A further decrease in the nanoflake size was observed in the neural pH, acidic and basic environmental conditions with Alg route.

Thermogravimetric analysis

Nanorod

Differential thermal analysis

Morphology

10.1080/1539445x.2019.1672192

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Citations (16)

Uptake, translocation, and physiological effects of hematite (α-Fe2O3) nanoparticles in barley (Hordeum vulgare L.)

Environmental Pollution (2020)

Hüseyin Tombuloğlu Y. Slimani Thamer Alshammari Muhammed Bargouti Mehmet Özdemir

10.1016/j.envpol.2020.115391

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Citations (55)

Isolation, characterization and fingerprinting of some chlorpyrifos- degrading bacterial strains isolated from Egyptian pesticides-polluted soils

African Journal of Microbiology Research (2011)

Nabil S. Awad Hussein Sabit E M Abo Aba Salah Abduallah Reda

Five bacterial isolates (B-CP5- B-CP6 - B-CP7- B-CP8- B-CP9) were isolated from pesticides- contaminated soil in Egypt. The capability of these isolates to degrade chlorpyrifos was investigated using enrichment mineral salt (MS) medium containing chlorpyrifos. Two different PCR-based techniques, RAPD-PCR and PCR-RFLP for amplified16S rRNA fragment were used to conduct genetic fingerprinting and obtain specific molecular markers for the studied isolates. The isolates exhibited substantial growth in mineral salt medium supplemented with 100-300 mg/L chlorpyrifos as a sole source of carbon and energy. Based on their morphological, cultural and biochemical characters, the isolates have been identified as Pseudomonas stuzeri, Enterobacter aerogenes, Pseudomonas pseudoalcaligenes, Pseudomonas maltophila andPseudomonas vesicularis respectively. Pseudomonas stuzeri was the most potent degrader strain. Five specific markers for this strain were determined. The highest genetic similarity was observed between CP8 and CP7 (66%), while the lowest genetic similarity was detected between CP8 and CP6 (37%). All isolates had the same pattern after digestion of 16S rRNA amplified fragment with two restriction enzymes (EcoRI and AluI) except Enterobacter aerogenes, which generate two monomorphic bands at 420 and 130 bp, respectively. In conclusion, the strain Pseudomonas stuzeri could be used to clean up the areas contaminated with Chlorpyrifos. Obtained molecular markers might be used for identifying and tracking the most potent bacterial isolate. The used PCR techniques represent a powerful tool and could be used for rapid typing of this strain. Key words: Pesticide, chlorpyrifos, biodegradation, 16S rRNA, RAPD-PCR,Pseudomonas sp.

Enterobacter aerogenes

Strain (injury)

10.5897/ajmr11.044

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Citations (56)