Aaron M. Neiman

Stony Brook University

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Hideki Nakanishi

Jiangnan University

Jae-Sook Park

Stony Brook University

Nancy M. Hollingsworth

Stony Brook University

Yasuyuki Suda

RIKEN Center for Advanced Photonics

Hiroyuki Tachikawa

Rikkyo University

Rolf Sternglanz

Nova Southeastern University

Alison Coluccio

University of Oregon

Mark E. Nickas

Stony Brook University

Kai Zhang

Chinese Academy of Fishery Sciences

Ira Herskowitz

University of California, San Francisco

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Stony Brook University

Harvard University

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State University of New York

Centre National de la Recherche Scientifique

Howard Hughes Medical Institute

The University of Tokyo

University of California, San Francisco

University of North Carolina at Chapel Hill

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A conserved regulatory role for antisense RNA in meiotic gene expression in yeast

Current Opinion in Microbiology (2011)

Huei-Mei Chen Aaron M. Neiman

Transcription

10.1016/j.mib.2011.09.010

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Citations (17)

Genetic dissection of the biochemical activities of RecBCD enzyme.

Genetics (1990)

Susan K. Amundsen Aaron M. Neiman S M Thibodeaux Gerald R. Smith

Abstract RecBCD enzyme of Escherichia coli is required for the major pathway of homologous recombination following conjugation. The enzyme has an ATP-dependent DNA unwinding activity, ATP-dependent single-stranded (ss) and double-stranded (ds) DNA exonuclease activities, and an activity that makes a ss DNA endonucleolytic cut near Chi sites. We have isolated and characterized ten mutations that reduced recombination proficiency and inactivated some, but not all, activities of RecBCD enzyme. One class of mutants had weak ds DNA exonuclease activity and lacked Chi-dependent DNA cleavage activity, a second class lacked only Chi-dependent DNA cleavage activity, and a third class retained all activities tested. The properties of these mutants indicate that the DNA unwinding and ss DNA exonuclease activities of the RecBCD enzyme are not sufficient for recombination. Furthermore, they suggest that the Chi-dependent DNA cleavage activity or another, as yet unidentified activity or both are required for recombination. The roles of the RecBCD enzymatic activities in recombination and exclusion of foreign DNA are discussed in light of the properties of these and other recBCD mutations.

RecBCD

10.1093/genetics/126.1.25

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Citations (78)

A phosphatidylinositol transfer protein integrates phosphoinositide signaling with lipid droplet metabolism to regulate a developmental program of nutrient stress–induced membrane biogenesis

Molecular Biology of the Cell (2014)

Jihui Ren Coney P. C. Lin Manish C. Pathak Brenda Temple Aaron H. Nile

Lipid droplet (LD) utilization is an important cellular activity that regulates energy balance and release of lipid second messengers. Because fatty acids exhibit both beneficial and toxic properties, their release from LDs must be controlled. Here we demonstrate that yeast Sfh3, an unusual Sec14-like phosphatidylinositol transfer protein, is an LD-associated protein that inhibits lipid mobilization from these particles. We further document a complex biochemical diversification of LDs during sporulation in which Sfh3 and select other LD proteins redistribute into discrete LD subpopulations. The data show that Sfh3 modulates the efficiency with which a neutral lipid hydrolase-rich LD subclass is consumed during biogenesis of specialized membrane envelopes that package replicated haploid meiotic genomes. These results present novel insights into the interface between phosphoinositide signaling and developmental regulation of LD metabolism and unveil meiosis-specific aspects of Sfh3 (and phosphoinositide) biology that are invisible to contemporary haploid-centric cell biological, proteomic, and functional genomics approaches.

Organelle biogenesis

Lipid droplet

Second messenger system

10.1091/mbc.e13-11-0634

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Citations (73)

Long-Chain Polyprenols Promote Spore Wall Formation in Saccharomyces cerevisiae

Genetics (2017)

Reuben Hoffmann Kariona A. Grabińska Ziqiang Guan William C. Sessa Aaron M. Neiman

Abstract Dolichols are isoprenoid lipids of varying length that act as sugar carriers in glycosylation reactions in the endoplasmic reticulum. In Saccharomyces cerevisiae, there are two cis-prenyltransferases that synthesize polyprenol—an essential precursor to dolichol. These enzymes are heterodimers composed of Nus1 and either Rer2 or Srt1. Rer2-Nus1 and Srt1-Nus1 can both generate dolichol in vegetative cells, but srt1∆ cells grow normally while rer2∆ grows very slowly, indicating that Rer2-Nus1 is the primary enzyme used in mitotically dividing cells. In contrast, SRT1 performs an important function in sporulating cells, where the haploid genomes created by meiosis are packaged into spores. The spore wall is a multilaminar structure and SRT1 is required for the generation of the outer chitosan and dityrosine layers of the spore wall. Srt1 specifically localizes to lipid droplets associated with spore walls, and, during sporulation there is an SRT1-dependent increase in long-chain polyprenols and dolichols in these lipid droplets. Synthesis of chitin by Chs3, the chitin synthase responsible for chitosan layer formation, is dependent on the cis-prenyltransferase activity of Srt1, indicating that polyprenols are necessary to coordinate assembly of the spore wall layers. This work shows that a developmentally regulated cis-prenyltransferase can produce polyprenols that function in cellular processes besides protein glycosylation.

Dolichol

Chitin synthase

Prenyltransferase

10.1534/genetics.117.300322

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Citations (21)

Interaction between VPS13A and the XK scramblase is required to prevent VPS13A disease in humans

bioRxiv (Cold Spring Harbor Laboratory) (2022)

Jae-Sook Park Yiying Hu Nancy M. Hollingsworth Gábriel Miltenberger-Miltényi Aaron M. Neiman

Abstract VPS13 family proteins form conduits between the membranes of different organelles through which lipids are transferred. In humans there are four VPS13 paralogs each of which is required to prevent a different inherited disorder. VPS13 proteins contain multiple conserved domains. The VAB domain binds to adaptor proteins to recruit VPS13 to specific membrane contact sites. This work demonstrates the importance of a different domain in VPS13A in preventing VPS13A disease (chorea-acanthocytosis). The Pleckstrin Homology (PH) domain at the C-terminus of VPS13A is required to form a complex with the XK scramblase and for proper localization of VPS13A within the cell. Mutations in the interaction surface between VPS13A and XK predicted by Alphafold modeling disrupt complex formation and colocalization of the two proteins. Mutant VPS13A alleles found in patients with VPS13A disease truncate the PH domain. The phenotypic similarities between VPS13A disease and McLeod syndrome caused by mutations in XK argue that loss of VPS13A-XK complex is the basis of both diseases. Summary Statement VPS13A disease and McLeod syndrome are related disorders caused by mutation of the VPS13A and XK genes, respectively. A pathologic VPS13A mutation disrupts binding of the VPS13A and XK proteins, suggesting a common basis of both diseases.

Pleckstrin homology domain

Phospholipid scramblase

10.1101/2022.05.09.491223

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Citations (3)

Phosphorylation of the MEKK Ste11p by the PAK-like kinase Ste20p is required for MAP kinase signaling in vivo

Current Biology (2000)

Frank van Drogen Sean M. O’Rourke Volker M. Stucke Malika Jaquenoud Aaron M. Neiman

MAP2K7

Phosphorylation cascade

ASK1

MAPKAPK2

MAPK14

10.1016/s0960-9822(00)00511-x

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Citations (160)

Unconventional Constituents and Shared Molecular Architecture of the Melanized Cell Wall of C. neoformans and Spore Wall of S. cerevisiae

Journal of Fungi (2020)

Christine Chrissian Coney Pei-Chen Lin Emma Camacho Arturo Casadevall Aaron M. Neiman

The fungal cell wall serves as the interface between the cell and the environment. Fungal cell walls are composed largely of polysaccharides, primarily glucans and chitin, though in many fungi stress-resistant cell types elaborate additional cell wall structures. Here, we use solid-state nuclear magnetic resonance spectroscopy to compare the architecture of cell wall fractions isolated from Saccharomyces cerevisiae spores and Cryptococcus neoformans melanized cells. The specialized cell walls of these two divergent fungi are highly similar in composition. Both use chitosan, the deacetylated derivative of chitin, as a scaffold on which a polyaromatic polymer, dityrosine and melanin, respectively, is assembled. Additionally, we demonstrate that a previously identified but uncharacterized component of the S. cerevisiae spore wall is composed of triglycerides, which are also present in the C. neoformans melanized cell wall. Moreover, we identify a tyrosine-derived constituent in the C. neoformans wall that, although it is not dityrosine, is a non-pigment constituent of the cell wall. The similar composition of the walls of these two phylogenetically distant species suggests that triglycerides, polyaromatics, and chitosan are basic building blocks used to assemble highly stress-resistant cell walls and the use of these constituents may be broadly conserved in other fungal species.

10.3390/jof6040329

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Citations (24)

Cdc15 Is Required for Spore Morphogenesis Independently of Cdc14 in Saccharomyces cerevisiae

Genetics (2007)

M Evangelina Pablo-Hernando Yolanda Arnáiz‐Pita Hideki Nakanishi Dean Dawson Francisco del Rey

In Saccharomyces cerevisiae exit from mitosis requires the Cdc14 phosphatase to reverse CDK-mediated phosphorylation. Cdc14 is released from the nucleolus by the Cdc14 early anaphase release (FEAR) and mitotic exit network (MEN) pathways. In meiosis, the FEAR pathway is essential for exit from anaphase I. The MEN component Cdc15 is required for the formation of mature spores. To analyze the role of Cdc15 during sporulation, a conditional mutant in which CDC15 expression was controlled by the CLB2 promoter was used. Cdc15-depleted cells proceeded normally through the meiotic divisions but were unable to properly disassemble meiosis II spindles. The morphology of the prospore membrane was aberrant and failed to capture the nuclear lobes. Cdc15 was not required for Cdc14 release from the nucleoli, but it was essential to maintain Cdc14 released and for its nucleo-cytoplasmic transport. However, cells carrying a CDC14 allele with defects in nuclear export (Cdc14-DeltaNES) were able to disassemble the spindle and to complete spore formation, suggesting that the Cdc14 nuclear export defect was not the cause of the phenotypes observed in cdc15 mutants.

Mitotic exit

10.1534/genetics.107.076133

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Citations (26)

Faculty Opinions recommendation of Insights into autophagosome biogenesis from structural and biochemical analyses of the ATG2A-WIPI4 complex.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature (2018)

Aaron M. Neiman

Autophagosome

10.3410/f.733956676.793551108

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Faculty Opinions recommendation of Programmed cortical ER collapse drives selective ER degradation and inheritance in yeast meiosis.