Rodrigo Arruda de Oliveira

Universidade de São Paulo

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I. Pivato

Universidade de Brasília

Mariane Leão Freitas

Universidade de Brasília

Marco Antônio de Oliveira Viu

Universidade Federal de Jataí

Maria Lúcia Gambarini

Universidade Federal de Goiás

Francisco José Gonçalves de Oliveira

Centro Universitário São Lucas

Margot Alves Nunes Dode

Universidade de Brasília

Christine Aurich

University of Veterinary Medicine Vienna

Hélio Blume

Faculdades Integradas de São Paulo

Jéssica Maresch de Araújo

Brazilian Agricultural Research Corporation

Tatiana Almeida Pignataro

Universidade de Brasília

Cooperative Institutions

Universidade de Brasília

Universidade Estadual Paulista (Unesp)

Universidade Federal de Goiás

Universidade de São Paulo

Brazilian Agricultural Research Corporation

Fundação de Estudo e Pesquisa em Medicina Veterinária e Zootecnia

Universidade Federal do Rio Grande do Sul

Instituto de Medicina Integral Professor Fernando Figueira

Universidade Federal de Minas Gerais

Universidade Brasil

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Cio do potro: o que é e quando utilizar?

Revista Brasileira de Reprodução Animal (2017)

Bárbara Souza Barros Barros Rodrigo Arruda de Oliveira

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#031 Tumor de Warthin – Relato de um caso clínico

Revista Portuguesa de Estomatologia Medicina Dentária e Cirurgia Maxilofacial (2022)

Gabriela Pinheiro Nuno Duarte Rodrigo Arruda de Oliveira Ana Cláudia Mariana Magalhães

esquelética severa, o tratamento ortodôntico -cirúrgico é a opção de tratamento mais adequada para

10.24873/j.rpemd.2022.12.916

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Avaliações in vitro da fertilidade do sêmen equino

PubVet (2015)

Rodrigo Arruda de Oliveira

10.22256/pubvet.v7n26.1646

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Liofilização celular e sua aplicação na reprodução animal

Revista Brasileira de Reprodução Animal (2015)

Adriane Bianchi Pedroni Medeiros Rodrigo Arruda de Oliveira I. Pivato

Lyophilization, or freeze-drying, is an alternative method of cellular preservation. This practice can assist the preservation of sperm cells, facilitating the storage and also the domestic or international transport, by eliminating the need of liquid nitrogen for storage. Lyophilization can maintain sperm viable for several years when stored at 4o C, and for hours at room temperature. In addition to exempting the use of liquid nitrogen, also has the advantage of allowing sperm to be separated into many aliquots for later use, even when the sperm stored in straws is lyophilized. What you can simplify and expand the use of freeze-dried spermatozoa in assisted reproduction techniques. Therefore, this paper aimed to describe the importance of lyophilization as a method of sperm preservation for animal reproduction, discussing the biotech history, the physical processes involved, the damages arising, the application, as well as their advantages and limitations.

Liquid nitrogen

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SUPEROVULAÇÃO EM ÉGUAS DA RAÇA CRIOULA E QUARTO DE MILHA COM EXTRATO DE PITUITÁRIA EQUINA (EPE)

Ciência Animal Brasileira (2019)

Deise Keli Farias Silvério Bunn Aimê de Medeiros Friso André Thaler Neto Alceu Mezzalira

Resumo Este trabalho compara a resposta superovulatória de éguas doadoras de embriões das raças Quarto de Milha (QM) e Crioula, utilizando um protocolo com baixa dose de extrato de pituitária equina (EPE). Oito éguas QM e seis éguas Crioulas foram acompanhadas durante 3 ciclos estrais consecutivos, sendo que cada ciclo corresponde a um grupo. Grupo Controle – monitoramento do crescimento folicular até a ovulação; Grupo EPE – monitoramento ultrassonográfico até que os folículos atingissem cerca de 20 mm de diâmetro e, posteriormente, administração de 7 mg de EPE, duas vezes ao dia, até a indução da ovulação com 2500UI de hCG; Grupo Pós-EPE – idem ao grupo controle. Os dados foram submetidos à análise de variância, utilizando o procedimento GLM do pacote estatístico SAS, sendo previamente testadas para normalidade dos resíduos, e as médias comparadas pelo teste de Tukey ao nível de 5%. Nas éguas Crioulas tratadas com EPE houve um aumento no número de ovulações (p=0,0534), com média de 3,33±2,06 quando comparadas aos grupos controle e pós-EPE e às éguas da raça QM (2,00±0,53). O tratamento com EPE na dose preconizada permitiu uma melhor resposta superovulatória e produção embrionária nas éguas Crioulas, quando comparadas com as éguas Quarto de Milha.

10.1590/1089-6891v20e-37582

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TERMOGRAFIA COMO MÉTODO DE AVALIAÇÃO DA TEMPERATURA CORPORAL E TESTICULAR DE GARANHÕES E SUAS CONSEQUÊNCIAS NA QUALIDADE SEMINAL.

Instituto Scientia eBooks (2022)

Thiago Rodrigues Cardoso Braga Rodrigo Arruda de Oliveira Mariane Leão Freitas

sobre esses fatores, e avaliar a eficácia do uso da termografia como método de predição da qualidade espermática.

10.55232/10830015.39

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Uterine Inflammatory Response After Prostaglandin E1 (Misoprostol) Infusion Prebreeding or Immediately After Embryo Flushing in Commercial Donor Mares

Journal of Equine Veterinary Science (2022)

G Amorim Lorenzo Segabinazzi Odilon Marquez de Oliveira Simone Perecmanis Rodrigo Arruda de Oliveira

Flushing

Uterine horns

10.1016/j.jevs.2022.104060

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Cooling of equine semen at 16ºC for 36h with the addition of cysteine in different concentrations

Pferdeheilkunde Equine Medicine (2015)

Rodrigo Arruda de Oliveira C.A. Wolf Marco Antônio de Oliveira Viu Maria Lúcia Gambarini

Equine semen manipulation during the cooling process reduces sperm viability and fertility in consequence to, among others, membrane lipid peroxidation, because of the high content of polyunsaturated fatty acids, which makes cells highly susceptible to free radicals and reactive oxygen species.The objective ofthe present study was to evaluate the effect of in vitro addition of cysteine in four concentrations (0, 1, 1.5, and 2.5 mM) for cooling spermatozoa of 12 stallions at 16 ºC for 36 h.Evaluated variables were total motility, strength, viability and plasmatic and acrosomal membrane integrity at four different time points (0, 12, 24 and 36 h).With the exception of acrosomal integrity, it was verified a reduction in total motility, strength and plasmatic membrane integrity in all samples, during cooling.In the evaluations at 36 h of cold storage, total motility (mot) and viability (viab) were higher in groups treated with 1mM (mot:46,5 ± 6,1/viab:76,5 ± 6,9) and 1.5 mM (mot:46,0 ± 4,6/viab:76,9 ± 3,7) cysteine, respectively, compared to control (mot:35,5 ±18,4/viab:68,1±13,4) and 2.5mM (mot:39,7 ±12,4/viab:66 ±17,2) (P < 0.05).As for strength (str) and plasmatic membrane integrity (plasm), 1 mM cysteine (str:3.6 ± 0.5/plasm:57.2± 9.5) showed better results compared to control (str:3.2±1.1/plasm:54.1±11.8),1.5 mM (str:3.5 ± 0.6/plasm:52.2±13.3) and 2.5 mM (str:3.2 ±1.1/plasm:55.8±12.5) (P < 0.05).Regarding acrosomal membrane integrity, in general, there was no loss of integrity (70.5 ±10.4; 69.4 ± 4.4; 68.0 ± 7.2 and 70.3 ± 0.5), control, 1 mM, 1.5 mM and 2.5 mM respectively.The concentration of 1mM cysteine was more efficient for the protection of sperm cells in the commercial system of passive cooling at 16 ºC for 36 h, with higher values for total motility, strength, viability and plasmatic membrane integrity.

10.21836/pem20150104

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Criopreservação de sêmen equino, um desafio a ser vencido

PubVet (2015)

Rodrigo Arruda de Oliveira

10.22256/pubvet.v7n26.1647

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ÍNDICE DE PRENHEZ COM SÊMEN CONGELADO DE GARANHÕES DA RAÇA CRIOULA USANDO GLICEROL OU DIMETILFORMAMIDA COMO CRIOPROTETORES

Ciência Animal Brasileira (2013)

Rodrigo Arruda de Oliveira Mara Iolanda Batistella Rubin Carlos Antônio Mondino Silva

Pregnancy rates using stallions’s frozen semen are variable; moreover, some breeds present low freezability. One hundred and four (104) mares were inseminated and separeted into two experiments to evaluate fertility of frozen semen’s of Criollo stallions (n=5), with 5% of dimethylformamide (DMF) or 5% of glycerol (GLY) as cryoprotectants. In Experiment I, the inseminations were made during pre-ovulation with fresh and frozen semen with DMF. In Experiment II, the mares were inseminated during post-ovulation with fresh semen, DMF and GL. The inseminations with frozen semen were performed deep in the uterine horn ipsilateralis to the dominant follicle. Control mares were inseminated with fresh semen in the uterus. Only one estrus period per mare was used to evaluate the pregnancy rates of the groups. Pregnancy diagnosis through ultrasonography was performed on the 15th day post-ovulation. The mean post-thawing motility was 40% and 20%, respectively, for frozen semen with DMF and GLY (P<0.05). All stallions had higher post-thawing motility when using DMF. In Experiment I, pregnancy rates were 12% (5/42) and 62% (20/32), respectively for DMF and fresh semen (P<0.0001). In Experiment II, pregnancy rates were 40% (4/10), 10% (1/10) and 70% (7/10), respectively for DMF, GLY (P<0.05) and fresh semen (P<0.0001). Insemination with frozen semen performed with more frequent follicular control showed the best results. The low sperm motility after thawing was attributed to the GLY used in experiment II. The DMF can be used alternatively to Criollos Stallion’s semen feezing.KEYWORDS: Artificial insemination; cryopreservation; cryoprotectant; equine; spermatozoa.

Cryoprotectant

10.5216/cab.v14i4.18923

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Citations (7)