A method has been developed for release/isolation of O-glycans from glycoproteins in whole cell lysates for mass spectrometric analysis. Cells are lysed in SDS, which is then exchanged for urea and ammonium bicarbonate in a centrifugal filter, before treating with NH4OH to release O-glycans. Following centrifugation, O-glycans are recovered in the filtrate. Sonication achieves O-glycan release in 1 h. Combining the established protocol for filter-aided N-glycan separation, here optimized for enhanced PNGase F efficiency, with the developed O-glycan release method allows analysis of both N- and O-glycans from one sample, in the same filter unit, from 0.5 to 1 million cells. The method is compatible with subsequent analysis of the residual protein by liquid chromatography-mass spectrometry (LC-MS) after glycan release. The medium throughput approach is amenable to analysis of biological replicates, offering a simple way to assess the often subtle changes to glycan profiles accompanying differentiation and disease progression, in a statistically robust way.

PNGase F

Ammonium bicarbonate

N-linked glycosylation

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Online Learning and Adaptation for Intelligent Embedded Agents Operating in Domestic Environments

Studies in fuzziness and soft computing (2003)

Hani Hagras Vic Callaghan Martin Colley Graham S. Clarke Hakan Duman

Scope (computer science)

Autonomous agent

10.1007/978-3-7908-1767-6_11

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A basic program to determine outliers in biopharmaceutical analysis using the Nair criterion

Journal of Pharmaceutical and Biomedical Analysis (1988)

Graham S. Clarke Malcolm L. Robinson

Replicate

Bioanalysis

Statistical Analysis

Sample (material)

10.1016/0731-7085(88)80059-1

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Dissociation between pancreatic enzyme secretory and synthetic dose-responses to cholecystokinin in man

International Journal of Pancreatology (1986)

E. J. S. Boyd J.K. Dunbar Graham S. Clarke K. G. Wormsley

The effect of varying the intensity of pancreatic stimulation on the synthesis of human pancreatic enzymes has not previously been studied. We have measured the secretion and synthesis of pancreatic enzymes in response to either secretin alone (1 Cu·kg−1·h−1) or secretin plus increasing doses of cholecystokinin (CCK) (0.25, 0.5 or 1.0 IDU·kg−1·h−1). Enzyme synthesis was measured using the incorporation of75Se-methionine (0.15 mCi (5.6 kBq)·kg−1·h−1) into the trichloracetic acid-insoluble fraction of the duodenal aspirate. Outputs of trypsin, chymotrypsin, lipase and protein showed a bell-shaped dose response to increasing doses of cholecystokinin, with maximal outputs occurring in response to secretin plus cholecystokinin 0.5 IDU·kg−1·h−1. The rate of incorporation of75Se-methionine increased with increasing doses of cholecystokinin and was maximal in response to secretin plus cholecystokinin 1.0 IDU·kg−1·h−1. There was therefore dissociation between the secretory and synthetic responses to increasing doses of cholecystokinin.

Secretin

10.1007/bf02795237

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The American Dream and American Cinema in the Age of Trump

Graham S. Clarke Ross Clarke

The American Dream and American Cinema in the Age of Trump uses both film theory and insights from object relations theory in order to examine how recent films address and reflect the state of the 'American Dream'. This fascinating book looks at how the American Dream is one of the organising ideas of American cinema, and one of the most influential cultural outputs of the twenty-first century, at a time of internal crisis. In an era characterised by populism, climate change and economic uncertainty, the book considers nine auteur films in how they illustrate the challenges of contemporary America. Graham S. Clarke and Ross Clarke present a bifocal perspective on some of the most well-received American films of recent years and how they relate to the American Dream in the context of the Trump presidency. For each of the nine films discussed, two different accounts are presented side by side so that each film is considered from an object relations psychoanalytic point of view (internal world) as well as a film and cultural theory perspective (external world). This unique approach is complemented by discussion of political and critical theory, providing a thorough and engaging analysis. Challenging and insightful, The American Dream and American Cinema in the Age of Trump will be of great interest to scholars of cinema, popular culture, American studies and psychoanalytic studies.

10.4324/9781003206224

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The Revenant (Iñárritu, 2015)

Routledge eBooks (2022)

Graham S. Clarke Ross Clarke

10.4324/9781003206224-4

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Us (Peele, 2019)

Routledge eBooks (2022)

Graham S. Clarke Ross Clarke

10.4324/9781003206224-11

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Filmography

Routledge eBooks (2022)

Graham S. Clarke Ross Clarke

Filmography

10.4324/9781003206224-15

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A modest proposal: Fairbairn’s psychology of dynamic structure is not “between paradigms” but already a synthesis of classical and relational thinking

Routledge eBooks (2018)

Graham S. Clarke

In this chapter, the author attempts here at a reinterpretation of Fairbairn's theory as already being a synthesis of classical and relational thinking before relational psychoanalysis was "invented" by Stephen Mitchell and Greenberg will provoke incredulity. He believes that Fairbairn's theory can be interpreted in such a way as to demonstrate this synthesis. The author thinks he is right in claiming that it is bad experience that leads to the establishment of internal objects as differentiated structures. He describes that the three ego structures would seem, characteristically, to be much more organised and differentiated—a phenomenon which may be attributed to the simple fact that they are ego-structures. The author argues that Fairbairn's theory of dynamic structure and the endopsychic system of inner reality, while based in classical thinking, when understood fully is founded upon a thoroughgoing relational approach, and as such it is a true and original synthesis of the two approaches.

10.4324/9780429452772-7

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