Eleazar Lara‐Padilla

Instituto Politécnico Nacional

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Cindy Bandala

Instituto Politécnico Nacional

Alexander Kormanovski

Instituto Politécnico Nacional

Noemí Cárdenas‐Rodríguez

Instituto Nacional de Pediatria

Gustavo Guevara‐Balcázar

Instituto Politécnico Nacional

ÁM

Ángel Miliar-García

Centro Médico Nacional La Raza

Francisco José Berral de la Rosa

Universidad Pablo de Olavide

Rafael Campos‐Rodríguez

Instituto Politécnico Nacional

Esaú Floriano-Sánchez

Hospital Central Militar

Alfonso Alfaro-Rodríguez

Instituto Nacional de Rehabilitación

Cleva Villanueva

Instituto Politécnico Nacional

Cooperative Institutions

Instituto Politécnico Nacional

228

Universidad Nacional Autónoma de México

Tecnológico Nacional de México

Secretaria de Salud

Center for Research and Advanced Studies of the National Polytechnic Institute

Mexican Social Security Institute

Centro Medico Nacional Siglo XXI

Universidad Pablo de Olavide

Instituto Nacional de Rehabilitación

Instituto Nacional de Enfermedades Respiratorias

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Anorectic interaction and safety of 5-hydroxytryptophan/carbidopa plus phentermine or diethylpropion in rat

Behavioural Pharmacology (2021)

Ernesto Limón-Bernal José Eduardo Roa‐Coria Ángel Zúñiga‐Romero Juan Carlos Huerta-Cruz Irma R.C. Ruíz-Velasco

Drug combinations are being studied as potential therapies to increase the efficacy or improve the safety profile of weight loss medications. This study was designed to determine the anorectic interaction and safety profile of 5-hydroxytryptophan (5-HTP)/carbidopa + diethylpropion and 5-HTP/carbidopa + phentermine combinations in rats. The anorectic effect of individual drugs or in combination was evaluated by the sweetened milk test. Isobologram and interaction index were employed to determine the anorectic interaction between 5-HTP/carbidopa and diethylpropion or phentermine. Plasma serotonin (5-HT) was measured by ELISA. Safety of repeated doses of both combinations in rats was evaluated using the tail sphygmomanometer, cardiac ultrasound, hematic biometry and blood chemistry. A single oral 5-HTP, diethylpropion or phentermine dose increased the anorectic effect, in a dose-dependent fashion, in 12 h-fasted rats. A dose of carbidopa at 30 mg/kg reduced the 5-HTP-induced plasmatic serotonin concentration and augmented the 5-HTP-induced anorectic effect. Isobologram and interaction index indicated a potentiation interaction between 5-HTP/30 mg/kg carbidopa + diethylpropion and 5-HTP/30 mg/kg carbidopa + phentermine. Chronic administration of experimental ED 40 of 5-HTP/30 mg/kg carbidopa + phentermine, but not 5-HTP/30 mg/kg carbidopa + diethylpropion, increased the mitral valve leaflets area. Moreover, there were no other significant changes in cardiovascular, hematic or blood parameters. Both combinations induced around 20% body weight loss after 3 months of oral administration. Results suggest that 5-HTP/30 mg/kg carbidopa potentiates the anorectic effect of diethylpropion and phentermine with an acceptable safety profile, but further clinical studies are necessary to establish their therapeutic potential in the obesity treatment.

Carbidopa

Anorectic

Phentermine

10.1097/fbp.0000000000000625

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Effects on secretory IgA levels in small intestine of mice that underwent moderate exercise training followed by a bout of strenuous swimming exercise

Brain Behavior and Immunity (2012)

Marycarmen Godínez‐Victoria Ma. Elisa Drago-Serrano Humberto Reyna-Garfias María Viloria Eleazar Lara‐Padilla

Polymeric immunoglobulin receptor

Lamina propria

Immunoglobulin A

10.1016/j.bbi.2012.07.018

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Citations (25)

Hipoglicemia durante el nado largo. El factor alimentario

Revista Médica Del Hospital General De México (2002)

Alexander Kormanovski Eleazar Lara‐Padilla Eduardo Díaz-Franco Jacinto Licea-Mendoza Fileno Piñera-Limas

Carbohydrate Metabolism

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Catecholamine levels and gene expression of their receptors in tissues of adults with osteosarcoma

Archives of Physiology and Biochemistry (2019)

Cindy Bandala Alberto Ávila-Luna Modesto Gómez‐López Eréndira Estrada‐Villaseñor Sergio Montes

The purpose of this work was to identify and measure catecholamines, their metabolites, and the gene expression of catecholamine receptors in osteosarcoma tissue.The levels of 3,4-dihydroxyphenylacetic acid, norepinephrine, serotonin, and 5-hydroxyindoleacetic acid in cancer tissue and in adjacent and non-oncological bone tissue were analysed by high-performance liquid chromatography, and the gene expression of catecholamine receptors and of dopamine β-hydroxylase, monoaminoxidase, ki67, and Runx2 in the osteosarcoma tissue, tissue adjacent to the tumour, non-oncological bone, and human brain tissue was analysed by RT-PCR.We found significantly higher levels of 3,4-dihydroxyphenylacetic acid and norepinephrine in the cancer sample than in adjacent and non-oncological bone. We found that β-adrenergic receptors and dopaminergic receptors, dopamine β-hydroxylase, ki67, Runx2, and serotonergic receptor gene expression were significantly higher in tumour tissue than in adjacent and non-oncological bone.Catecholamines and their receptors could be potential molecular markers for osteosarcoma progression.

10.1080/13813455.2019.1638942

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Biosensor of inflammation biomarkers based on electrical bioimpedance analysis on immobilized DNA without chemical modification

Journal of Electrical Bioimpedance (2020)

Modesto Gómez‐López Ángel Miliar-García Nadia Mabel Pérez-Vielma Eleazar Lara‐Padilla César Antonio González-Díaz

The development of biosensors to identify molecular markers or specific genes is fundamental for the implementation of new techniques that allow the detection of specific Deoxyribonucleic acid (DNA) sequences in a fast, economic and simple way. Different detection techniques have been proposed in the development of biosensors. Electrical Bioimpedance Spectroscopy (EBiS) has been used for diagnosis and monitoring of human pathologies, and is recognized as a safe, fast, reusable, easy and inexpensive technique. This study proves the development of a complementary DNA (cDNA) biosensor based on measurements of EBiS and DNA's immobilization with no chemical modifications. The evaluation of its potential utility in the detection of the gene expression of three inflammation characteristic biomarkers (NLRP3, IL-1β and Caspase 1) is presented. The obtained results demonstrate that EBiS can be used to identify different gene expression patterns, measurements that were validated by Quantitative Polymerase Chain Reaction (qPCR). These results indicate the technical feasibility for a biosensor of specific genes through bioimpedance measurements on the immobilization of cDNA.

10.2478/joeb-2020-0006

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Effect of botulinum neurotoxin type A (BoNTA) on the morphology and viability of 3T3 murine fibroblasts.

PubMed (2015)

Cindy Bandala Juan Luis Terán-Melo Maricruz Anaya-Ruı́z César M. Mejía-Barradas R Dominguez-Rubio

BoNTA is used in the treatment of ophthalmological disorders, muscular hyperactivity and pain. In recent years it has been described that BoNTA reduces cellular viability and induces apoptosis in prostate cells lines. Studies about the effect of BoNTA are no well known. There have been studies about the effect of BoNTA on the expression levels of collagenase in fibroblasts, but not on its morphological impact on these cells. The aim of this study was to determine the effect of BoNTA on the morphology and viability of the 3T3 fibroblast cell line.The 3T3 fibroblast cell line was cultured and the experimental group received 10 U BoNTA added to a 0.9% sterile saline solution in a reconstituted vial. The control group received saline solution only. Cultured cells were observed and photographed at 5, 10, 15 and 20 h. Cell viability was evaluated by means of the trypan blue test, and cell proliferation with the Proliferation Assay kit (PROMEGA).The application of BoNTA to 3T3 fibroblast cells induced morphological changes, such as a loss of normal fibroblast morphology. Additionally, we observed the cytoplasmic retraction and spread phenomena. The nuclei showed other important changes with Giemsa staining.The results indicate that BoNTA induced a loss of spindle form, increase in cytoplasmic vesicles, and the presence of nuclear vesicles (compacted chromatin surrounded by a nuclear envelope). This suggests an apoptotic process and decreased cell viability. Further studies are needed to explore the mechanisms of these alterations.

Viability assay

Trypan blue

3T3 cells

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Citations (8)

Oxidative stress and vascular reactivity changes in thoracic and abdominal aorta expose at the hyperbaric oxygenation (693.1)

The FASEB Journal (2014)

Nallely Acevedo‐Villavicencio Alexander Kormanovsky Cleva Villanueva Israel Ramírez‐Sánchez Eleazar Lara‐Padilla

During the hyperbaric oxygenation therapy (HBO) has been described some changes like hypoglycemia, low blood pressure, etc. The mean idea of this work is to analyze if the HBO induce some changes in the vascular reactivity and oxidative stress in healthy rats. Methods: Male rats were used (3 months old) and divided in 2 groups: with and without HBO.Rats were exposed to pure oxygen at 2 absolute atmospheres during 60 min for 3, 5, 10 and 20 sessions, after that, we obtain thoracic and abdominal aorta and were used some of the aortic rings for the isolated organ system where we made concentration response curves; and other aortic rings were used to analyze the oxidative stress. Results: changes were observed in the contractile effect to phenyleprine in both segments after HBO, without any changes in the relaxant response. On the other hand thoracic oxidative response were higher after 3 sessions of HBO, but in an interesting way we can observe that antioxidant enzyme decreased after this time; those results suggest that the antioxidant system increase the response trying to metabolize O2. Grant Funding Source : COFAA / IPN

Abdominal aorta

Thoracic aorta

Hyperbaric oxygenation

Reactivity

10.1096/fasebj.28.1_supplement.693.1

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The consumption of n-3 polyunsaturated fatty acids differentially modulates gene expression of peroxisome proliferator-activated receptor alpha and gamma and hypoxia-inducible factor 1 alpha in subcutaneous adipose tissue of obese adolescents

Endocrine (2013)

César M. Mejía-Barradas Blanca E. Del‐Rio‐Navarro Aarón Domínguez‐López Rafael Campos‐Rodríguez María de los Ángeles Martínez–Godínez

10.1007/s12020-013-9941-y

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Citations (42)

Comparative treatment with hyperbaric oxygen therapy in a model of systemic loxoscelism in rats.

DOAJ (DOAJ: Directory of Open Access Journals) (2022)

Mireille Toledo-Blas Antonio Franco‐Vadillo Selma Alin Somilleda-Ventura Brenda Dominguez-Ruiz Gustavo Guevara‐Balcázar

Spiders of the Loxosceles genus, known as violin spiders, produce venom with dermonecrotic and systemic effects, as it is a species widely distributed in the world, its study represents a high medical relevance. Systemic loxoscelism, which occurs in 1 in 5 cases and is the most frequent in children, can be fatal, so the study of effective therapy is of great relevance. In the present study, we compared different therapeutic options to mitigate the systemic effects of Loxosceles boneti venom in a model in which prepubertal rats were used.A model of systemic intoxication by L. boneti venom was provoked in male Wistar rats. Study groups were formed: healthy control, with venom and untreated control, treatment with N-acetylcysteine, and/or hyperbaric oxygenation therapy. Subsequently, pathological analysis of the kidney and lung was performed. The oxidant-antioxidant response was evaluated, and molecular analysis of the COX-1 and COX-2 enzymes was performed.Regenerative changes were observed at the cellular level in both treatments, being more noticeable in the hyperbaric oxygen therapy (HBO) group. The anti-oxidant response was outstanding in the same group.Both treatments offer considerable benefits, however; further studies are needed to provide adequate therapeutics.

Hyperbaric oxygenation

Therapeutic effect

Antidote

10.22038/ijbms.2022.65357.14385

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Effect of caloric restriction and aerobic exercise on the celular immune response of the intestinal mucous in mice

Eleazar Lara‐Padilla Francisco José Berral de la Rosa Adriana Jarillo‐Luna Guillermo López‐Lluch Plácido Navas

Caloric theory

Aerobic Exercise

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