Prasad Reddy

Indian Veterinary Research Institute

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Co-Authors

Osnat Herzberg

Advanced Bioscience Laboratories (United States)

Alan Peterkofsky

National Institutes of Health

Jonathan Reizer

University of California, San Diego

Milton H. Saier

University of California, San Diego

Keith McKenney

George Mason University

Andrew Howard

Illinois Institute of Technology

Sarah L. Sutrina

University of the West Indies

Miral Dizdaroğlu

National Institute of Standards and Technology

Vani Rao Chalamasetty

National Institute of Standards and Technology

Wojciech Krajewski

The Francis Crick Institute

Cooperative Institutions

National Institute of Standards and Technology

Biotechnology Institute

National Institutes of Health

Advanced Bioscience Laboratories (United States)

Southampton General Hospital

University of Maryland, Baltimore

University of New Mexico

University of Maryland, College Park

National Heart Lung and Blood Institute

National Institute of Standards

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Structure of HI1333, a Hypothetical Protein from Haemophilus influenzae with Structural Similarity to RNA-binding Proteins

M.A. Willis Wojciech Krajewski Vani Rao Chalamasetty Prasad Reddy Anthony Howard

Similarity (geometry)

Structural alignment

10.2210/pdb1jo0/pdb

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Citations (0)

Effect of PDI Overexpression on Recombinant Protein Secretion in CHO Cells

Biotechnology Progress (2000)

Roger J. Davis Kenneth Schooley Brian Schou Rasmussen James N. Thomas Prasad Reddy

In eukaryotic cells, protein disulfide isomerase (PDI) found in the endoplasmic reticulum (ER) catalyzes disulfide bond exchange and assists in protein folding of newly synthesized proteins. PDI also functions as a molecular chaperone and has been found associated with proteins in the ER. In addition, PDI functions as a subunit of two more complex enzyme systems: the prolyl-4-hydroxylase and the triacylglycerol transfer proteins. Increasing PDI activity in bacterial, yeast, and insect cell expression systems can lead to increased secretion of heterologous proteins containing disulfide bridges. Since Chinese hamster ovary (CHO) cells are widely used for the expression of recombinant proteins, we expressed recombinant human PDI (rhu PDI) in CHO cells to increase cellular PDI levels and examined its effect on the secretion of two different recombinant proteins: interleukin 15 (IL-15) and a tumor necrosis factor receptor:Fc fusion protein (TNFR:Fc). Secretion of TNFR:Fc (a disulfide-rich protein) is decreased in cells overexpressing PDI; the TNFR:Fc protein is retained inside these cells and colocalizes with the overexpressed rhu PDI protein in the endoplasmic reticulum. PDI overexpression did not result in intracellular retention of IL15. The nature of the interaction between PDI and TNFR:Fc was further investigated by expressing a disulfide isomerase mutant PDI in CHO cells to determine if the functional activity of PDI is involved in the cellular retention of TNFR:Fc protein.

Chaperone (clinical)

Foldase

10.1021/bp000107q

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Citations (104)

Factors Regulating the Activity of Escherichia Coli Adenylate Cyclase

Springer eBooks (1985)

Prasad Reddy E S Liberman Celia Gazdar Alan Peterkofsky

10.1007/978-94-011-6565-5_23

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Citations (3)

Hemodynamic Effects of Butorphanol-Oxygen Anesthesia in Dogs

Anesthesia & Analgesia (1981)

James Sederberg Theodore H. Stanley Prasad Reddy Wen-Shin Liu J. David Port

The cardiovascular effects of two intravenous rates of butorphanol infusion (0.1 and 0.2 mg/kg/min) were measured in 32 dogs breathing oxygen and nitrous oxide in oxygen. Sixteen dogs were premedicated with atropine and the other 16 were unpremeditated. Of the dogs receiving the higher dose infusion rate, 25% moved with a tail-clamp stimulus whereas 75% moved with the lower doses. Butorphanol produced significant but similar cardiovascular depression with the two rates of infusion irrespective of the presence or absence of atropine medication. Addition of nitrous oxide resulted in further cardiac depression in all groups studied. The data suggest that butorphanol is not an attractive alternative to morphine or fentanyl as a narcotic anesthetic.

Butorphanol

Nitrous oxide

Narcotic

10.1213/00000539-198110000-00003

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Citations (16)

Nutritional strategies for amelioration of heat stress in dairy cows: A Review

International Journal of Veterinary Sciences and Animal Husbandry (2023)

Prasad Reddy P Manoj Kumar Goud Divya Katam Ratna Supriya Mani Kumar

The livestock sector provides a consistent income to the farmer and acts as a backbone of the national economy along with agriculture and in the near future, this sector will become the major source to provide nutritional security (meat and milk) for the growing population. But the changing climate scenario and global warming are increasing the effect of Heat stress (HS) on animals and it becomes a severe threat to sustainable livestock production. HS compromises the growth, production, and reproduction efficiency of dairy cattle and will cause severe economic losses to the dairy farmer. The adverse effects of HS can be minimized by three types of interventions including genetic manipulation, nutritional interventions, and management practices that help the dairy cow to sustain production and reproduction in hot weather. Among these, nutritional strategies are simple and cost-effective in preventing the adverse effects of HS. This article will discuss various nutritional strategies such as the provision of ad libitum cold water, quality protein, fat, minerals, vitamins, and other feed additives necessary for dairy cows to cope with HS and sustain productivity in dairy cows.

Dairy Farming

10.22271/veterinary.2023.v8.i3sa.613

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Citations (1)

Structure of HI1333 (YhbY), a putative RNA‐binding protein from Haemophilus influenzae

Proteins Structure Function and Bioinformatics (2002)

M.A. Willis Wojciech Krajewski Vani Rao Chalamasetty Prasad Reddy Andrew Howard

10.1002/prot.10225

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Citations (10)

A Rapid Method for the Isolation of Genomic DNA From Aspergillus Fumigatus

Preparative Biochemistry (1995)

Nivedita Bir Anubha Paliwal K. Muralidhar Prasad Reddy P. Usha Sarma

A majority of Aspergillus induced diseases are reported to be caused by Aspergillus fumigatus. In immunocompromized and post transplant cases it can lead to invasive aspergillosis. Due to this the molecular fingerprinting of aspergillus isolates by RFLP analysis and development of DNA diagnostic probes are gaining importance. Different methodologies are being adopted for extraction of the genomic DNA from fungus. The existing procedures for isolation of DNA are time consuming and range from several hours to few days. The most difficult step in the isolation of DNA from aspergillus species is to disrupt the tough chitin rich cell wall without causing damage to genomic DNA. We report here a rapid method for extraction of genomic DNA based on the cleavage of chitin with chitinase. The subsequent modification steps included are lysis and microwave treatment. The chromosomal DNA obtained by this procedure is 1.5-2.0 micrograms per mg of wet weight of mycelia and is observed to be minimally sheared. It is pure enough for restriction analysis and for use in the PCR to detect the gene coding for 18 kDa allergen which has been identified in our laboratory using western blot analysis with human patient sera.

genomic DNA

Southern blot

Chitinase

10.1080/10826069508010119

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Citations (26)

Crystallization of the IIA domain of the glucose permease of Bacillus subtilis

Journal of Molecular Biology (1991)

Geeta Kapadia Celia C. H. Chen Prasad Reddy Milton H. Saier Jonathan Reizer

PEP group translocation

Ammonium sulfate

Phosphotransferases

10.1016/0022-2836(91)90919-w

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Citations (6)

Abstract 3891: Identification and quantification of DNA repair proteins by liquid chromatography/isotope-dilution tandem mass spectrometry using 15N-labeled whole proteins as internal standards

Cancer Research (2011)

Miral Dizdaroğlu Paweł Jaruga Prasad Reddy

Abstract Reduced DNA repair capacity is associated with increased risk for a variety of disease processes including carcinogenesis. Thus, DNA repair proteins have the potential to be used as important predictive, prognostic and therapeutic biomarkers in cancer and other diseases. The measurement of the expression level of these enzymes may be an excellent tool for this purpose. Mass spectrometry is becoming the technique of choice for the identification and quantification of proteins. We applied liquid chromatography/ isotope-dilution tandem mass spectrometry (LC-MS/MS) for the identification and quantification of DNA repair proteins human 8-oxoguanine-DNA glycosylase (hOGG1) and E. coli formamidopyrimidine DNA glycosylase (Fpg), which are involved in base-excision repair of DNA damage. We over-expressed, purified and characterized 15N-labeled analogues of these proteins to be used as internal standards. 15N-labeled whole proteins are ideal internal standards to ensure the accuracy of quantification of proteins by mass spectrometry. DNA glycosylase activities of 15N-labeled hOGG1 and 15N-labeled Fpg were determined and found to be essentially identical to those of their respective unlabeled counterparts, ascertaining that the 15N-labeling did not perturb their catalytic sites. hOGG1, Fpg and their 15N-labeled analogues were digested with trypsin and analyzed by LC-MS/MS. A large number of tryptic peptides of both proteins were identified on the basis of their full-scan mass spectra. These peptides matched the theoretical peptide fragments expected from the trypsin digestion and provided statistically significant protein scores that would unequivocally identify these proteins. We also recorded the product ion spectra (MS/MS spectra) of the tryptic peptides and defined the characteristic product ions. Mixtures of the analyte proteins and their 15N-labeled analogues as internal standards were analyzed by selected-reaction monitoring (SRM) on the basis of the previously identified product ions in the MS/MS spectra. The experimental conditions for these analyses were optimized. The methodology was successfully applied to the measurement of human OGG1 in human cells and Fpg in E.coli. The results obtained in this work suggest that the methodology developed would be highly suitable for the positive identification and accurate quantification of DNA repair proteins in vivo as potential biomarkers for cancer and other diseases. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 3891. doi:10.1158/1538-7445.AM2011-3891

Isotope dilution

Label-free Quantification

Bottom-up proteomics

Tandem mass tag

10.1158/1538-7445.am2011-3891

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Citations (1)

Improved Method for the Production of M13 Phage and Single-Stranded DNA for DNA Sequencing

BioTechniques (1996)

Prasad Reddy Keith McKenney

An improved method is described for the efficient production of M13 phage and M13 single-stranded (ss)DNA in a relatively short time period. Infection of E. coli (F') cells with as few as 5 phage particles can yield 10(12) phage particles/mL in 3 hours if the cells are grown in LB broth or SOB broth supplemented with about 5 mM Mg2+. The method tolerates large variations in the initial multiplicity of infection (5-5000 phage per 5 x 10(7) cells) and still yields about 10(12) phage particles/mL. These amounts are sufficient to purify 10-15 micrograms of ssDNA and to carry out at least 10-15 DNA sequencing reactions.

Multiplicity of infection

Phagemid

10.2144/96205st05

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Citations (19)