A 56‐year‐old woman came to the hospital with fever and skin eruptions. A rise in myogenic enzyme and the presence of antileucocyte antibody were noticed, along with the gradual appearance of myalgia in both lower extremities, and muscle weakness. Steroid therapy was started under the diagnosis of polymyositis. The steroid was reduced because of mental disturbance but immediately the patient developed high fever. Various forms of treatment were carried out but there was no improvement, and the patient died. At autopsy there were scattered purpura on the skin, and the muscles were atrophic and yellowish‐grey in color. Histopathologically, there was inflammatory cell infiltration and muscle fiber degeneration visible in many of the muscles, and the findings showed evidence of polymyositis. There were intranuclear inclusions in the lungs, ovaries, and adrenal glands, and this was diagnosed as generalized cytomegalic inclusion disease. Fibrin thrombi were found in the kidneys, lungs, and adrenal glands and this was pathologically diagnosed as disseminated intravascular coagulation. Endothelial cell damage caused by cytomegalovirus was assumed to be involved to a large extent in triggering the disseminated intravascular coagulation. ACTA PATHOL. JPN. 35: 723–730, 1985.
We describe a case of solitary papilloma of the bronchus and provide a review of 38 similar cases reported in Japan. A 70-year-old man complained of cough and sputum. Chest X-rays and CT scans revealed atelectasis of the right middle lobe. On bronchoscopy, a polypoid tumor was found at the orifice of the bronchus of the right middle lobe. The tumor was histologically diagnosed as a squamous papilloma with moderate atypia. Because of elevated tumor markers and the reported high incidence of malignant changes in papillomas, the tumor was endoscopically resected by electrosurgical snare. While this procedure resulted in improvement of atelectasis, the chest CT taken subsequently revealed a mass adjacent to the resected polypoid tumor in the middle lobe bronchus. Percutaneous needle biopsy followed by histopathological examination confirmed the tumor to be a squamous cell carcinoma. Only three cases of malignant changes in papillomas have been previously reported in Japan. Electrosurgical snare, which allows the identification of tissue at the tumor base, should be the treatment of choice rather than YAG laser surgery.(Internal Medicine 40: 56-60, 2001)
Eosinophilic cytoplasmic inclusion bodies were observed in the midbrain, pons, medulla and spinal cord of beef cattle (Japanese Brown). The inclusion bodies, observed in 14 of 20 cases exhibiting neurological signs and in one of nine healthy cases, differed in morphology and/or staining properties from previously reported inclusion bodies in humans and animals.
Three females in their fifties and five males in their seventies, all of whom had been exposed to the Nagasaki atomic bomb explosion in 1945, were investigated concerning the aging of neurons of the dorsal root ganglia. Through this study dealing with the frequency of neurons with intracytoplasmic pigments, lipofuscin, neuromelanin, eosinophilic granules and neurons free of pigment, no significant difference in four kinds of neurons between the exposed persons and the nonexposed persons was detected in 50-59 year-old females. Contrariwise in the 70-79 year-old males, the frequency, as average but not as individual persons, was significantly higher in lipofuscin, lower in neuromelanin, and unchanged in eosinophilic granules and no pigment.
Abstract Aim Dedifferentiated fat (DFAT) cells, similar to mesenchymal stem cells (MSCs), are gaining attention owing to their minimally invasive nature. We aimed to evaluate the effects of 3D DFAT spheroids on bone regeneration by implantation into rat skull defects. Methods Adipose tissue was harvested from 8‐week‐old Sprague–Dawley rats, and DFAT was prepared using the ceiling culture method. DFAT was seeded onto three‐dimensional (3D) culture plates to produce spheroids, enhance cellular interactions, and mimic the in vivo environment. Bone defects were created on the skulls of 15‐week‐old rats using a 6.0‐mm trephine burr. Collagen sponges were transplanted as controls, and 3D DFAT spheroid‐seeded sponges were used in the experimental group. Results DFAT showed a fibroblast‐like morphology. Flow cytometry revealed that DFAT expressed MSC markers but not hematopoietic stem cell markers. Bone analysis revealed increased bone mineral density, volume, and trabecular thickness in the experimental group. Hematoxylin–eosin staining in the control group revealed only a thin layer of fibrous tissue, whereas the experimental group showed new bone tissue formation. The control group lacked Von Willebrand factor (vWf) expression, whereas the experimental group exhibited vWf expression, indicating the development of new blood vessels. Von Kossa staining revealed calcification only in the experimental group. Conclusion Spheroids alone are difficult to retain in bone defects, making their use in combination with collagen sponges more effective for bone regeneration. The study findings suggest that 3D DFAT spheroids may help regenerate rat skull defects, with potential implications for human bone regeneration.
A case of urethral diverticulum with calculus in a 53-year-old woman is reported. She was admitted to Isahaya General Hospital complaining of a localized swelling in the external genitalia. We found a urethral diverticular calculi by urethrography. Urethral diverticulectomy was carried out without any complications. The removed stones were in 2 pieces and they were composed of calcium phosphate and ammonium urate.
Male Wistar rats weighing 280g were subjected to the stress condition in which the rats were thrusted unceasingly and harassingly with a bamboo stick by the reseacher, for twenty minutes a day for a period of three and twelve months. The blood pressure reached a high mean value (191±3 S. E.) in twelve months. Mean organweight to body-weight ratios of heart, kidney and adrenal gland when compared to nonstressed controls were increased, and that of thymus was decreased. One of the animals subjected to the stress developed significant vascular pathology in the form of panarteritis like arteriopathy and simple fibrous intimal thickening, and others disclosed nothing of vascular changes. The analysis of relative thickness of media to radius in the branches of coronary arteries resulted in no change between subjected rats and controls in thickness of media, compared to that of spontaneously hypertensive reats (SHR) which disclosed medial hypertrophy.
