Gonçalo Dória

Stab Vida (Portugal)

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Pedro V. Baptista

Universidade Nova de Lisboa

Ricardo Franco

Fundação Oswaldo Cruz

Eulália Pereira

Rede de Química e Tecnologia

Orfeu Flores

Centro Medico Nacional Siglo XXI

Elvira Fortunato

Universidade Nova de Lisboa

Rodrigo Martins

Universidade Nova de Lisboa

Leandro Raniero

Universidade do Vale do Paraíba

João Conde

Universidade Nova de Lisboa

Inês Gomes

University of Lisbon

Eduardo Coelho

Stab Vida (Portugal)

Cooperative Institutions

Universidade Nova de Lisboa

University of Lisbon

Rede de Química e Tecnologia

University of Eastern Finland

Stab Vida (Portugal)

Hospital Garcia de Orta

Universidade do Porto

Faculdade de Tecnologia e Ciências

Madan Parque

Instituto Politécnico de Lisboa

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Star-shaped magnetite@gold nanoparticles for protein magnetic separation and SERS detection

RSC Advances (2013)

Pedro Quaresma Inês Osório Gonçalo Dória P.A. Carvalho André M. Pereira

A novel synthetic methodology for star shaped gold-coated magnetic nanoparticles is reported. The coating is performed in two steps: formation of gold nuclei at the surface of magnetite nanoparticles followed by growth of the gold nuclei into a complete star shaped shell. The star-shaped gold-coated magnetic nanoparticles thus obtained preserve the magnetic properties of the precursor magnetite nanoparticles, e.g. they can be easily separated with a magnet. In addition, the gold coating provides interesting optical properties while simultaneously allowing for biofunctionalization that may be advantageous for biological applications, such as (bio)detection via surface-enhanced Raman spectroscopy (SERS). As a proof-of-concept, a capping agent terminated with a nickel(II)-nitrilotriacetate group showing high affinity for histidine was used to modify the surface of the nanoparticles. The resulting star-shaped nanoparticles were used to selectively capture histidine-tagged maltose-binding protein from a crude cell extract. Finally, the performance of star shaped gold-coated magnetic nanoparticles as SERS platforms was demonstrated through the detection of Raman active dye (Astra Blue).

Magnetite Nanoparticles

Surface-Enhanced Raman Spectroscopy

Surface Modification

10.1039/c3ra46762g

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Citations (100)

Portable optoelectronic biosensing platform for identification of mycobacteria from the Mycobacterium tuberculosis complex

Biosensors and Bioelectronics (2010)

Leonardo Bione Silva Bruno Veigas Gonçalo Dória Pedro Costa João Inácio

SIGNAL (programming language)

10.1016/j.bios.2010.08.078

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Alloy metal nanoparticles for multicolor cancer diagnostics

Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE (2011)

Pedro V. Baptista Gonçalo Dória João Conde

Cancer is a multigenic complex disease where multiple gene loci contribute to the phenotype. The ability to simultaneously monitor differential expression originating from each locus results in a more accurate indicator of degree of cancerous activity than either locus alone. Metal nanoparticles have been thoroughly used as labels for in vitro identification and quantification of target sequences. We have synthesized nanoparticles with assorted noble metal compositions in an alloy format and functionalized them with thiol-modified ssDNA (nanoprobes). These nanoprobes were then used for the simultaneous specific identification of several mRNA targets involved in cancer development - one pot multicolor detection of cancer expression. The different metal composition in the alloy yield different "colors" that can be used as tags for identification of a given target. Following a non-cross-linking hybridization procedure previously developed in our group for gold nanoprobes, these multicolor nanoprobes were used for the molecular recognition of several different targets including differently spliced variants of relevant genes (e.g. gene products involved in chronic myeloid leukemia BCR, ABL, BCR-ABL fusion product). Based on the spectral signature of mixtures, before and after induced aggregation of metal nanoparticles, the correct identification could be made. Further application to differentially quantify expression of each locus in relation to another will be presented. The differences in nanoparticle stability and labeling efficiency for each metal combination composing the colloids, as well as detection capability for each nanoprobe will be discussed. Additional studies will be conducted towards allele specific expression studies.

Nanoprobe

10.1117/12.879025

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An isothermal lab-on-phone test for easy molecular diagnosis of SARS-CoV-2 near patients and in less than 1 hour

International Journal of Infectious Diseases (2022)

Gonçalo Dória Carla Clemente Eduardo Coelho João Colaço Rui Crespo

The performance of a new point-of-care CE-IVD-marked isothermal lab-on-phone COVID-19 assay was assessed in comparison to a gold standard real-time reverse transcriptase-PCR method.The study was conducted following a nonprobability sampling of ≥16-year-old volunteers from three different laboratories, using direct mouthwash (N = 24) or nasopharyngeal (N = 191) clinical samples.The assay demonstrated 95.19% sensitivity and 100% specificity for detection of SARS-CoV-2 in direct nasopharyngeal crude samples and 78.95% sensitivity and 100% specificity in direct mouthwash crude samples. It also successfully detected currently predominant SARS-CoV-2 variants of concern (Beta B.1.351, Delta B.1.617.2, and Omicron B.1.1.529) and demonstrated to be inert against potential cross-reactions of other common respiratory pathogens that cause infections that present similar symptoms to COVID-19.This lab-on-phone pocket-sized assay relies on an isothermal amplification of SARS-CoV-2's N and E genes, taking just 50 minutes from sample to result, with only 2 minutes of hands-on time. It presents good performance when using direct nasopharyngeal crude samples, enabling a low-cost, real-time, rapid, and accurate identification of SARS-CoV-2 infections at the point of care, which is important for both clinical management and population screening, as a tool to break the chain of transmission of COVID-19 pandemic, especially in low-resources environments.

Gold standard (test)

Point-of-Care Testing

Point of care

10.1016/j.ijid.2022.07.042

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Noble Metal Nanoparticles for Biosensing Applications

Sensors (2012)

Gonçalo Dória João Conde Bruno Veigas Letícia Giestas Carina Almeida

In the last decade the use of nanomaterials has been having a great impact in biosensing. In particular, the unique properties of noble metal nanoparticles have allowed for the development of new biosensing platforms with enhanced capabilities in the specific detection of bioanalytes. Noble metal nanoparticles show unique physicochemical properties (such as ease of functionalization via simple chemistry and high surface-to-volume ratios) that allied with their unique spectral and optical properties have prompted the development of a plethora of biosensing platforms. Additionally, they also provide an additional or enhanced layer of application for commonly used techniques, such as fluorescence, infrared and Raman spectroscopy. Herein we review the use of noble metal nanoparticles for biosensing strategies--from synthesis and functionalization to integration in molecular diagnostics platforms, with special focus on those that have made their way into the diagnostics laboratory.

Noble metal

10.3390/s120201657

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Citations (672)

Novel Optoelectronic Platform using an Amorphous/Nanocrystalline Silicon Biosensor for the Specific Identification of Unamplified Nucleic Acid Sequences Based on Gold Nanoparticle Probes

TRANSDUCERS 2007 - 2007 International Solid-State Sensors, Actuators and Microsystems Conference (2007)

L. B. Silva Pedro V. Baptista Leandro Raniero Gonçalo Dória Ricardo Franco

Here we describe an innovative optoelectronic platform which enables the specific detection of unamplified nucleic acid sequences with the integration of oligonucleotide-derivatized gold nanoparticles, a colour sensor and a light emission source for a colorimetric detection method. This new low cost, fast and simple optoelectronic platform permits detection of less than 1 picomole quantities of nucleic acid without target or signal amplification.

Nanocrystalline material

10.1109/sensor.2007.4300285

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Citations (3)

Development of a fast and efficient ultrasonic-based strategy for DNA fragmentation

Talanta (2010)

Miguel Larguinho Hugo M. Santos Gonçalo Dória Holger C. Scholz Pedro V. Baptista

Sonication

Fragmentation

10.1016/j.talanta.2010.01.032

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Citations (26)

Gold nanoparticle-based fluorescence immunoassay for malaria antigen detection

Analytical and Bioanalytical Chemistry (2011)

Bassem S. Guirgis Cláudia Sá e Cunha Inês Gomes Miguel Cavadas Isabel Gonçalves Silva

10.1007/s00216-011-5489-y

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Citations (84)

Noble Metal Nanoparticles Applications in Cancer

Journal of Drug Delivery (2011)

João Conde Gonçalo Dória Pedro V. Baptista

Nanotechnology has prompted new and improved materials for biomedical applications with particular emphasis in therapy and diagnostics. Special interest has been directed at providing enhanced molecular therapeutics for cancer, where conventional approaches do not effectively differentiate between cancerous and normal cells; that is, they lack specificity. This normally causes systemic toxicity and severe and adverse side effects with concomitant loss of quality of life. Because of their small size, nanoparticles can readily interact with biomolecules both at surface and inside cells, yielding better signals and target specificity for diagnostics and therapeutics. This way, a variety of nanoparticles with the possibility of diversified modification with biomolecules have been investigated for biomedical applications including their use in highly sensitive imaging assays, thermal ablation, and radiotherapy enhancement as well as drug and gene delivery and silencing. Here, we review the available noble metal nanoparticles for cancer therapy, with particular focus on those already being translated into clinical settings.

Biomolecule

Cancer Therapy

Cancer Treatment

10.1155/2012/751075

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Citations (507)

Inkjet printed and “doctor blade” TiO2 photodetectors for DNA biosensors

Biosensors and Bioelectronics (2009)

Iwona Bernacka‐Wojcik G.K.R. Senadeera P. Wójcik Leonardo Bione Silva Gonçalo Dória

10.1016/j.bios.2009.09.027

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Citations (68)