Shaimaa Abdelmalik Pessar

Ain Shams University

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Rasha Abd El‐Rahman El‐Gamal

Ain Shams University

Nahela Ahmed Shalaby

Ain Shams University

Mariam John Amin Ibrahim

Ain Shams University

Raghda El Sayed Abdel Monem Galal

Ain Shams University

Mahira I. Elmogy

Ain Shams University

Marina Mamdouh Malky Ibrahim

Ain Shams University

Hanan M. Mahmoud

University of Tripoli

Dalia Ahmed El-Sewefy

Ain Shams University

Botheina Ahmed Thabet Farweez

Ain Shams University

Heba Samy Agamy

Ain Shams University

Cooperative Institutions

Ain Shams University

Ain Shams University Hospital

Al-Azhar University

Menoufia University

Armed Forces College of Medicine

National Center for Geriatrics and Gerontology

British Geriatrics Society

Zagazig University

Cairo University

Pediatrics and Genetics

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Urinary miRNA-377 and miRNA-216a as biomarkers of nephropathy and subclinical atherosclerotic risk in pediatric patients with type 1 diabetes

Journal of Diabetes and its Complications (2017)

Mona H. El-Samahy A. M. Adly Yasmine Ibrahim Elhenawy E. G. M. Ismail Shaimaa Abdelmalik Pessar

Albuminuria

Subclinical infection

Pathogenesis

10.1016/j.jdiacomp.2017.10.014

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Citations (39)

SCREENING PARADIGM FOR ?-THALASSEMIA CARRIERS; FROM CLINICAL TO MOLECULAR

DOAJ (DOAJ: Directory of Open Access Journals) (2014)

Ibrahim Youssef Soha R. Youssef Galila M. Mokhtar Mahira I. Elmogy Hanan M. Mahmoud

Paradigm shift

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Diagnostic Approach and risk stratification of thrombocytosis: Integrating morphological, cytogenetic features and MPL gene mutation

Menoufia Medical Journal (2023)

Olfat Hendy Dina Fouad Shaimaa Abdelmalik Pessar Sara M. Aboagiza Hanan M. Bedair

Objectives: To evaluate the integration of clinical, morphological, cytogenetic features and MPL gene mutation analysis in Egyptian patients with clonal thrombocytosis and correlate these finding to patient’s outcome. Background: Thrombocytosis can be caused by either reactive or autonomous processes as a result of genetic alterations that affect signaling pathway through gain-of-function activity of MPL (myeloproliferative leukemia virus), Janus kinase 2 (JAK 2), calreticulin (CALR). Methods: This study was conducted on 60 Egyptian patients having clonal thrombocytosis attending to clinical pathology department, Ain Shams University Hospitals during the period from August 2020 to December 2021. Complete blood count, LDH level and MPL gene mutation by Real-time PCR were measured. Results: JAK2 gene mutation was more prevalent than MPL gene mutation in our study (40% and 8.3% respectively). MPL mutation has an aggressive nature than JAK2 mutation as evident by; older age (68.80± 2.28 vs 55.5 ± 8.28 accordingly), more thrombotic manifestation (100% vs 58.3%), higher platelets count (1221.4 ± 309.70 vs 879.38 ± 215.98) and poor response to therapy (0% of MPL positive patients achieved complete response to therapy vs 41.7% for JAK2 positive patients) but with no marked morphological difference regarding megakaryocytes morphology. Conclusion: MPL W515L/K mutations present in 10.3% in essential thrombocythemia Egyptian patients, included in our current study. MPL gene mutation has a bad prognostic effect. Moreover, presence of coexisting JAK2 & MPL mutation have a synergistic bad effect to be evaluated in further larger studies.

Thrombocytosis

10.59204/2314-6788.1053

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Methyltransferase-like 3 gene (METTL3) expression and prognostic impact in acute myeloid leukemia patients

Egyptian Journal of Medical Human Genetics (2022)

Reham Mohamed Nagy Amal Abd El Hamid Mohamed Rasha Abd El‐Rahman El‐Gamal Shereen Abdel Monem Ibrahim Shaimaa Abdelmalik Pessar

Abstract Background DNA methylation is involved in pathogenesis of acute myeloid leukemia (AML). N6-methyladenosine (m6A) modification of mRNA, mediated by methyltransferase-like 3 ( METTL3 ), is one of the well-identified mRNA modifiers associated with the pathogenesis of AML. High level of METTL3 mRNA is detected in AML cells, thus can be a potential target therapy for AML. This is a preliminary study that aimed at measuring METTL3 mRNA expression level in de novo AML patients and correlating it with clinicopathological, laboratory and prognostic markers. METTL3 expression was analyzed by quantitative reverse transcription polymerase chain reaction in 40 newly diagnosed AML adults and was re-measured in the 2nd month of chemotherapy. Patients were followed up for periods up to 6 months post-induction therapy. Results METTL3 expression was found to be significantly upregulated in AML patients compared to control subjects ( p < 0.001). METTL3 gene was significantly expressed among non-responders compared to responders ( p < 0.001). A cutoff value was assigned for normalized METTL3 values to categorize AML patients according to response to therapy. Statistically significant association was observed between high pretreatment normalized METTL3 gene level and failure to attain complete remission at 2nd, 4th and 6th month following therapy ( p = 0.01, 0.02 and 0.003, respectively) . However, insignificant correlation was found between pretreatment normalized METTL3 gene level and event free survival or clinicopathological prognostic factors. Conclusion METTL3 is overexpressed in AML patients and is associated with adverse prognostic effect and failure to attain hematological remission within 6 months post-induction therapy.

Pathogenesis

10.1186/s43042-022-00242-8

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Prognostic implications of IDH1rs11554137 and IDH2R140Q SNPs mutations in cytogenetically normal acute myeloid leukemia

Egyptian Journal of Medical Human Genetics (2019)

Sahar Samir AbdElMaksoud Rasha Abd El‐Rahman El‐Gamal Shaimaa Abdelmalik Pessar Dalia Diaa ElDine Salem Hanaa Fathey Abdelsamee

Mutant isocitrate dehydrogenase (IDH) 1 and 2 alter the epigenetic landscape in acute myeloid leukemia (AML) cells through production of the oncometabolite (R)-2-hydroxyglutarate. We aimed to determine the prevalence and clinical and prognostic effect of IDH1rs11554137 and IDH2R140Q SNPs mutations, in 80 newly diagnosed cytogenetically normal AML (CN-AML), using real-time polymerase chain reaction (PCR). Heterozygous mutations of IDH1 and IDH2 SNP were detected in 13.8% and 16.3% of patients, respectively. Both mutations were associated with older age, higher platelet count, and shorter overall survival. Survivors showed significantly younger age and lower mean platelet and blast counts, as well as negative IDH1 SNP (p = 0.001, 0.016, 0.002, and 0.003, respectively). Multivariate logistic regression analysis identified high bone marrow blast percentage as an independent prognostic predictor for 6-month mortality (p = 0.014, OR 1.049, 95% CI 1.010–1.090). IDH1/2 SNPs mutations are recurrent events in CN-AML associated with negative prognostic impact, representing a new subgroup for risk stratification and may indicate new treatment options.

Isocitrate dehydrogenase

SNP

IDH2

10.1186/s43042-019-0012-7

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Erythroferrone, the new iron regulator: evaluation of its levels in Egyptian patients with beta thalassemia

Annals of Hematology (2019)

Rasha Abd El‐Rahman El‐Gamal Ibrahim Youssef Abdel-Messih Deena Mohamed Habashy Shams ElDoha Galal ElDin Zaiema Shaimaa Abdelmalik Pessar

Transferrin saturation

Beta thalassemia

10.1007/s00277-019-03882-w

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Pseudo Eosinophilia: A Role for Automation in Plasmodium Vivax Detection

Shaimaa Abdelmalik Pessar

A 52-year-old male, Sudanese, diagnosed to have vivax malaria while being investigated for anemia and fever of 3 months duration. Automated eosinophil count turned to be erroneously elevated discordant with manual count which attributed to presence of Plasmodia and its byproducts in circulation suggesting a role for automated complete blood count (CBC) in malaria diagnosis. Because general screening tests like CBC are always undertaken for patients who present with fever. Hematologists should be aware that samples containing malaria may give erroneously high eosinophil counts, thus blood films should be reviewed carefully and the White Blood Cell (WBC) differential count should be confirmed microscopically.

Blood film

Blood count

Complete blood count

White blood cell

Vivax malaria

Blood smear

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Diagnostic Evaluation of Hematological Sepsis Score and Presepsin in Neonatal Sepsis

Iranian Journal of Pediatric Hematology & Oncology (2022)

Marina Mamdouh Malky Ibrahim Dalia Ahmed El-Sewefy Mariam John Amin Ibrahim Shaimaa Abdelmalik Pessar

Background: Early detection of neonatal sepsis and categorization of patients based on clinical severity is not yet effectively achieved. Some hematological parameters are used to formulate a hematological scoring system (HSS) and a modified hematological scoring system (MHSS) to diagnose neonatal sepsis. A promising biomarker: Presepsin, or Soluble Cluster of Differentiation 14 SubType (sCD14-ST), is a proteolysis product of CD14 produced after immune activation during infections. The purpose of this research is to assess the performance of both hematological sepsis scores and serum presepsin level in neonatal sepsis and compare them to C-reactive protein (CRP) as diagnostic tools and predictors of mortality. Materials and Methods: This case-control study comprised two groups, one group comprised 51 neonates who were further subgrouped into suspected & proved sepsis, along with 30 uninfected neonates as the control group. Both groups were subjected to the calculation of HSS and MHSS, serum presepsin levels, CRP measurement, and blood culture and assessed for clinical severity and mortality. Results: Hematological sepsis scores and presepsin levels were significantly higher in the sepsis group (P <0.001). Presepsin showed the best diagnostic performance at > 0.5 ng/ml (AUC 0.979; sensitivity of 94.1% and specificity of 100%). While HSS and MHSS at a cutoff value > 1 achieved comparable specificity, lower sensitivity, 72.6% for the former and 76.5% for the later was noted. Presepsin also was significantly higher in the dead group (P<0.004) with the best predictive performance over CRP at cutoff value >1.9 ng/ml (AUC 0.838; sensitivity of 85.7% and specificity of 79.6%). Conclusion: Hematological sepsis scores and presepsin were useful diagnostic tools in neonatal sepsis, with presepsin as a good predictor of mortality comparable to CRP.

Neonatal Sepsis

Hematology

10.18502/ijpho.v12i2.9075

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Clinical Significance of Lymphoid Enhancer-Binding Factor 1 (LEF-1) Expression in Acute Myeloid Leukemia

The Egyptian Journal of Hospital Medicine (2018)

Nahela Ahmed Shalaby Doaa Ahmed Gamal Eissa Botheina Ahmed Thabet Farweez Shaimaa Abdelmalik Pessar Raghda El Sayed Abdel Monem Galal

Lymphoid enhancer-binding factor-1 (LEF1) is a member of the LEF/T-cell factor family of transcription factors and a key mediator of the Wingless-type (Wnt) pathway. It mediates Wnt signals through

Clinical Significance

Expression (computer science)

10.21608/ejhm.2018.9841

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Evaluation of polymorphonuclear leukocyte elastase levels in neonatal sepsis

International Journal of Research in Medical Sciences (2016)

Shaimaa Abdelmalik Pessar

Background: Sepsis is an important cause of neonatal morbidity and mortality. In Egypt, A multi-center study reported that 45.9% of the neonates admitted to neonatal intensive care units (NICUs) were due to suspected neonatal sepsis. Proper management is not guaranteed due to nonspecific symptoms and signs especially at the onset of disease, delay of culture results and high rate of false negative results. Attempts have been made to use hematologic parameters, acute phase reactants and cytokine profiles for early and accurate diagnosis of neonatal sepsis; however, none was adequately sensitive or specific. Polymorphonuclear elastase (PMN-E) is a serine protease secreted during inflammatory diseases. The aim of the current study was to explore the reliability of PMN-E level for the diagnosis of neonatal sepsis and assess response to treatment in comparison to hematological parameters and C - reactive protein (CRP).Methods: The study comprised 30 neonates with proven sepsis, 30 neonates as control group. Both groups were subjected to calculation of hematologic sepsis score (HSS), CRP measurement, blood culture and serum PMN-E levels, the latter was measured both at diagnosis and 6 days after treatment in sepsis group.Results: Serum PMN-E levels were significantly higher in sepsis group (118.4±21.8) than in control group (57.9±27.9) and the best cut-off value was at 85 ng/ml with 97% sensitivity and 81% specificity, PMN-E levels also decreased significantly with response to treatment.Conclusions: Raised PMN-E level was found to be a diagnostic and prognostic marker in neonates with sepsis comparable with CRP and HSS.

Neonatal Sepsis

Blood Culture

Neutrophil elastase

10.18203/2320-6012.ijrms20163795

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Citations (1)