Yuji Yaguchi

Ibaraki Prefectural Livestock Center

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Kiyoko Tamai

Hiromichi Suzuki

Tsukuba Medical Center Hospital

Tomoyuki SHIBAHARA

National Agriculture and Food Research Organization

Shigeyuki Notake

Tsukuba Medical Center Hospital

Yuki Kashima

Ibaraki Prefectural Livestock Center

Katsunori Yanagihara

Nagasaki University Hospital

Masanari Shiigai

Tsukuba Medical Center Hospital

Yusaku Akashi

Kindai University

Yuki Fujii

Okayama University Hospital

Haruhisa MASATOU

Hitachi (Netherlands)

Cooperative Institutions

National Institute of Animal Health

National Agriculture and Food Research Organization

Ibaraki Prefectural Livestock Center

University of Tsukuba

Tsukuba Medical Center Hospital

Hokkaido University

University of Tsukuba Hospital

Nihon University

The University of Tokyo

National Institute of Infectious Diseases

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Spatial transmission of H5N6 highly pathogenic avian influenza viruses among wild birds in Ibaraki Prefecture, Japan, 2016–2017

Archives of Virology (2018)

Ryota Tsunekuni Yuji Yaguchi Yuki Kashima Kaoru Yamashita Nobuhiro Takemae

10.1007/s00705-018-3752-7

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Citations (11)

Optimizing immunohistochemical staining for rapid diagnosis of porcine epidemic diarrhea

Japanese Journal of Large Animal Clinics (2016)

Yuji Yaguchi Shinichi Takahashi Yoji Komatsu Kenji KAWASHIMA

豚流行性下痢（PED）は，主に新生子豚の水様性下痢や高い致死率を示す急性ウイルス感染症である．PEDの診断には免疫組織化学的染色（免疫染色）による腸管病変部のウイルス抗原の検出が有効であるが，通常の手法では結果判定までに数日間の時間を要する．そこで迅速判定を目的として，迅速固定および凍結切片を用いたPEDの免疫染色の手法を検討した．迅速固定では，10％中性緩衝ホルマリン液を用いて温度や時間を変更した条件を設定してPED発症豚の小腸を固定し，免疫染色標本でのウイルス抗原の染色強度と分布を顕微鏡下で比較した．その結果，40℃および60℃の１時間加温固定が迅速固定としてPEDの免疫染色に最適であった．凍結切片を用いた手法では，-80℃に冷却したヘキサンで凍結後にクリオスタットにより薄切した切片をホルマリンメタノールで固定し，マイクロウェーブ処理による抗原賦活化法を用いてPEDの免疫染色を実施した．凍結切片を用いたPEDの免疫染色において，ウイルス特異抗原を検出し形態学的にも十分な染色結果が得られた．以上より，迅速固定および凍結切片を用いた免疫染色を実施することにより，解剖当日ないし翌日に判定可能な迅速なPEDの診断が可能となった．

10.4190/jjlac.7.163

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Swine Ulcerative Colitis Associated with <i>Entamoeba</i> spp.

Journal of the Japan Veterinary Medical Association (2016)

Yuji Yaguchi Makoto Matsubayashi Tomoko Itahashi Yuki Fujii Tokio ONUKI

削痩が認められた83日齢の肥育豚2頭について，病性鑑定を実施した．2頭ともに潰瘍性大腸炎が広範囲に認められ，病変部組織には多数のEntamoeba属原虫の栄養型が認められた．遺伝子解析の結果より，同原虫はE. poleckiサブタイプ3であった．病理学，細菌学及びウイルス学的検索から，この原虫が病態発現に関与したことが示唆された．

Entamoeba

10.12935/jvma.69.389

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Granulomatous Nephritis and Meningoencephalomyelitis Caused by Halicephalobus gingivalis in a Pony Gelding

Journal of Veterinary Medical Science (2007)

Masataka Akagami Tomoyuki SHIBAHARA Toyoshi Yoshiga Nobuaki Tanaka Yuji Yaguchi

A ten-year-old Shetland pony gelding showed low appetite, ataxia, peculiar swaying, clouding of consciousness, and ultimately died. At necropsy, multiple coalescing granulomatous foci were detected in the kidneys, and small necrotic lesions were found in the cerebellum. Histologic examination of the renal tissue sections revealed extensive granuloma, and Halicephalobus gingivalis-like nematodes were seen. Similar nematodes were found in the granulomatous or necrotic lesions of the renal lymph nodes and cerebellum, and were also frequently detected in cerebrospinal meningovascular lesions. Morphologic features together with partial ribosomal RNA gene sequences of the nematodes in the lesions revealed that they were H. gingivalis. The present results indicated that H. gingivalis caused granulomatous nephritis and meningoencephalomyelitis in this pony gelding.

Pony

Nephritis

10.1292/jvms.69.1187

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Citations (46)

Multicenter evaluation of the Verigene Gram-negative blood culture nucleic acid test for rapid detection of bacteria and resistance determinants in positive blood cultures

Diagnostic Microbiology and Infectious Disease (2015)

Naoki Uno Hiromichi Suzuki Hiromi Yamakawa Maiko Yamada Yuji Yaguchi

Blood Culture

Concordance

Nucleic acid test

10.1016/j.diagmicrobio.2015.08.004

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Citations (17)

Risk factors associated with the within-farm transmission of bovine viral diarrhea virus and the incidence of persistently infected cattle on dairy farms from Ibaraki prefecture of Japan

Research in Veterinary Science (2020)

Masataka Akagami Satoko Seki Yuki Kashima Kaoru Yamashita Shoko Oya

Seroprevalence

Cattle Diseases

10.1016/j.rvsc.2020.02.001

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Citations (25)

Pararenal Lymphatic Cyst Infection Caused by <i>Helicobacter cinaedi</i>

Internal Medicine (2015)

Yusaku Akashi Jun Igarashi Hiromichi Suzuki Emiko Rimbara Keigo Shibayama

A 43-year-old man was referred to our hospital for an acute-onset fever and left flank pain. He had been previously diagnosed with lymphangioma, and abdominal computed tomography showed pararenal cysts with fat stranding around the left kidney, of which infection was subsequently confirmed on magnetic resonance imaging. Gram-negative spiral bacilli were isolated from two sets of blood cultures, and Helicobacter cinaedi was identified using 16S rRNA sequencing. The patient was successfully treated with ceftriaxone therapy without recurrence. A multilocus sequence typing analysis indicated the current H. cinaedi strain differed from previous strains isolated in Japan.

Helicobacter

Lymphangioma

10.2169/internalmedicine.54.3991

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Enzootic bovine leukosis accompanied by splenomegaly in an 8-month-old calf

Journal of the Hellenic Veterinary Medical Society (2020)

Susumu Okada Kazumi Tagawa Ryan Wada Yuji Yaguchi Yuki Kashima

In this report, an 8-month-old calf (crossbred, Holstein × Japanese Black) developed fever and accompanied abomasum displacement. Blood chemical test showed remarkably high values of white blood cell count and heteromorphic lymphocytes. In pathological appraisal, enlarged splenomegaly and swelling of the lymph nodes were observed. Histopathological examination revealed invasion of tumor cells derived from B1 cells into systemic lymph nodes, liver and spleen. The provirus loads of bovine leukemia virus (BLV) was 1,439 copies per 10 ng DNA by using real time PCR. In conclusion, this case was diagnosed as bovine leukemia caused by BLV infection with a huge splenomegaly.

Bovine leukemia virus

Provirus

White blood cell

10.12681/jhvms.22969

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First case of a bloodstream infection caused by the genus Brachybacterium

Journal of Infection and Chemotherapy (2018)

Kiyoko Tamai Yusaku Akashi Yuta Yoshimoto Yuji Yaguchi Yosuke Takeuchi

Bloodstream infection

Isolation

10.1016/j.jiac.2018.06.005

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Citations (27)

Survey of bovine foamy virus infection among cattle in Japan and comparison with bovine leukemia virus infection

Journal of Veterinary Medical Science (2020)

Ryoji Iwasaki Yu NAKAGIRI Yuji Yaguchi K. Oguma Mamiko ONO

The prevalence of bovine foamy virus (BFV) infections in cattle on farms in the Kanto region of Japan was determined using agar gel immunodiffusion (AGID) test and polymerase chain reaction (PCR). Six out of 20 farms contained BFV-positive cattle. Furthermore, 16.7% (91/545) of all cattle tested positive for BFV. This suggested that BFV-infected cattle are widely prevalent in Japan. Positive results for BFV infection were consistent between AGID and PCR tests. Additionally, we tested for bovine leukemia virus (BLV) infections at nine farms, primarily those containing BFV-infected cows. At each farm, the infection rate of BFV was lower than that of BLV. Further, cattle that were PCR-positive but antibody-negative, indicating immune tolerance to BFV, were not detected.

Bovine leukemia virus

Cattle Diseases

10.1292/jvms.19-0592

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Citations (3)