A method for rapidly evaluating functional interactions between ligands and G-protein-coupled receptors has been developed. The technology is based on the ability of animals to change color by controlling the position of pigmented organelles within skin cells called melanophores. cDNA coding for a receptor to be studied is expressed in immortalized frog melanophores. Stimulation of a receptor that normally functions to activate either adenyl cyclase or phospholipase C induces centrifugal melanosome translocation and cell darkening. Conversely, application of an agonist to cells expressing a receptor that operates to inhibit adenyl cyclase induces centripetal pigment movement and cell lightening. The simple optical change can be used to investigate ligand-receptor interactions at several levels, including single-cell analysis and high-throughput chemical screening. Current efforts are focused on (1) identifying small peptides that activate or block thromboxane A2 and platelet-activating factor (PAF) receptors and (2) cloning eicosanoid receptors.
Journal Article Peripheral events in moth olfaction Get access Michael R. Lerner, Michael R. Lerner Section of Molecular Neurobiology, Yale University School of Medicine333 Cedar Street, P.O. Box 3333, New Haven, CT 06510, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Tunde K. Gyorgyi, Tunde K. Gyorgyi Section of Molecular Neurobiology, Yale University School of Medicine333 Cedar Street, P.O. Box 3333, New Haven, CT 06510, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Jeffrey Reagan, Jeffrey Reagan Section of Molecular Neurobiology, Yale University School of Medicine333 Cedar Street, P.O. Box 3333, New Haven, CT 06510, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Alison Roby-Shemkovitz, Alison Roby-Shemkovitz Section of Molecular Neurobiology, Yale University School of Medicine333 Cedar Street, P.O. Box 3333, New Haven, CT 06510, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Robert Rybczynski, Robert Rybczynski Section of Molecular Neurobiology, Yale University School of Medicine333 Cedar Street, P.O. Box 3333, New Haven, CT 06510, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Richard G. Vogt Richard G. Vogt Section of Molecular Neurobiology, Yale University School of Medicine333 Cedar Street, P.O. Box 3333, New Haven, CT 06510, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Chemical Senses, Volume 15, Issue 2, April 1990, Pages 191–198, https://doi.org/10.1093/chemse/15.2.191 Published: 01 April 1990
cDNA encoding pheromone-binding protein (PBP), the major soluble protein in olfactory sensilla of male moths, has been cloned from the tobacco hornworm, Manduca sexta. A study of the developmental time course of PBP reveals that it is first synthesized just prior to eclosion and that the percentage of antennal mRNA encoding PBP shifts from zero to about 20% at that time. PBP is also found in sensilla from female M. sexta antennae. No amino acid sequence homology is observed between PBP and the vertebrate odorant-binding protein.
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