Poh-Yam Lim

Nagoya University

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Takashi Akazawa

Niigata University

Junji Yamaguchi

Hokkaido University

Javier Pozueta‐Romero

Agrobiotechnology Institute

Pierdomenico Perata

Scuola Superiore Sant'Anna

Kazumi Aratani

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Agrobiotechnology Institute

Universidad Publica de Navarra

Nagoya University

Niigata University

Miyagi University

Renesas Electronics (Japan)

Hitachi (Japan)

Renesas Electronics (United States)

Hanazono University

Fundación Instituto Leloir

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Distinct Profiles of ADP- and UDP-Specific Sucrose Synthases in Developing Rice Grains

Bioscience Biotechnology and Biochemistry (1992)

Poh-Yam Lim Pierdomenico Perata Javier Pozueta‐Romero Takashi Akazawa Junji Yamaguchi

Journal Article Distinct Profiles of ADP- and UDP-Specific Sucrose Synthases in Developing Rice Grains Get access Poh-Yam Lim, Poh-Yam Lim Research Institute for Biochemical Regulation, School of Agricultural Sciences, Nagoya University, Chikusa, Nagoya 464-01, Japan Search for other works by this author on: Oxford Academic Google Scholar Pierdomenico Perata, Pierdomenico Perata Research Institute for Biochemical Regulation, School of Agricultural Sciences, Nagoya University, Chikusa, Nagoya 464-01, Japan Search for other works by this author on: Oxford Academic Google Scholar Javier Pozueta-Romero, Javier Pozueta-Romero Research Institute for Biochemical Regulation, School of Agricultural Sciences, Nagoya University, Chikusa, Nagoya 464-01, Japan Search for other works by this author on: Oxford Academic Google Scholar Takashi Akazawa, Takashi Akazawa Research Institute for Biochemical Regulation, School of Agricultural Sciences, Nagoya University, Chikusa, Nagoya 464-01, Japan Search for other works by this author on: Oxford Academic Google Scholar Junji Yamaguchi Junji Yamaguchi Research Institute for Biochemical Regulation, School of Agricultural Sciences, Nagoya University, Chikusa, Nagoya 464-01, Japan Search for other works by this author on: Oxford Academic Google Scholar Bioscience, Biotechnology, and Biochemistry, Volume 56, Issue 4, 1 January 1992, Pages 695–696, https://doi.org/10.1271/bbb.56.695 Published: 01 January 1992 Article history Received: 16 December 1991 Published: 01 January 1992

10.1271/bbb.56.695

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Isolation and Characterization of Nuclei from Rice Embryos.

Cell Structure and Function (1992)

Junji Yamaguchi Poh-Yam Lim Kazumi Aratani Takashi Akazawa

A method has been developed to isolate pure preparations of nuclei in high yield from commercially available viable rice embryos (germ), employing extraction with buffer solution containing glycerol (without detergent) and polyamine, followed by centrifugation on a 30% Percoll cushion. The intactness of the isolated nuclei was confirmed by light microscopy as well as electron microscopy. The protein profiles of both whole nuclei and nuclear extracts obtained by SDS-PAGE, organellar marker enzyme activities, DNA and RNA analyses, and in vitro RNA synthesis, all indicate that the highly purified nuclei are isolated from rice embryos.

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10.1247/csf.17.87

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