Natalia Gutiérrez

Andalusian Institute of Agricultural and Fisheries Research and Training

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A. M. Torres

Andalusian Institute of Agricultural and Fisheries Research and Training

C. M. Ávila

Andalusian Institute of Agricultural and Fisheries Research and Training

Carmen Palomino

The University of Texas at El Paso

Sergio G. Atienza

Instituto de Agricultura Sostenible

M. T. Moreno

Universidad de Sevilla

María J. Giménez

Instituto de Agricultura Sostenible

Diego Rubiales

Instituto de Agricultura Sostenible

ZŠ

Zlatko Šatović

University of Zagreb

J. I. Cubero

University of Córdoba

Cristina Rodríguez‐Suárez

Instituto de Agricultura Sostenible

Cooperative Institutions

University of California, Los Angeles

327

Loyola Marymount University

273

Banaras Hindu University

271

Andalusian Institute of Agricultural and Fisheries Research and Training

Instituto de Agricultura Sostenible

University of Córdoba

Consejo Superior de Investigaciones Científicas

Stanford University

University of California, San Francisco

Harvard University

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Marker-assisted selection in faba bean (Vicia faba L.)

Field Crops Research (2009)

A. M. Torres C. M. Ávila Natalia Gutiérrez Carmen Palomino M. T. Moreno

Marker-Assisted Selection

Molecular marker

Molecular breeding

Plant Breeding

Selectable marker

Ascochyta

10.1016/j.fcr.2008.12.002

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Citations (98)

Genome-wide association analysis for drought tolerance and associated traits in faba bean (Vicia faba L.)

Frontiers in Plant Science (2023)

Natalia Gutiérrez Marie Pégard Christiane Balko A. M. Torres

Faba bean ( Vicia faba L.) is an important high protein legume adapted to diverse climatic conditions with multiple benefits for the overall sustainability of the cropping systems. Plant-based protein demand is being expanded and faba bean is a good candidate to cover this need. However, the crop is very sensitive to abiotic stresses, especially drought, which severely affects faba bean yield and development worldwide. Therefore, identifying genes associated with drought stress tolerance is a major challenge in faba bean breeding. Although the faba bean response to drought stress has been widely studied, the molecular approaches to improve drought tolerance in this crop are still limited. Here we built on recent genomic advances such as the development of the first high-density SNP genotyping array, to conduct a genome-wide association study (GWAS) using thousands of genetic polymorphisms throughout the entire faba bean genome. A worldwide collection of 100 faba bean accessions was grown under control and drought conditions and 10 morphological, phenological and physiological traits were evaluated to identify single nucleotide polymorphism (SNP) markers associated with drought tolerance. We identified 29 SNP markers significantly correlated with these traits under drought stress conditions. The flanking sequences were blasted to the Medicago truncatula reference genomes in order to annotate potential candidate genes underlying the causal variants. Three of the SNPs for chlorophyll content after the stress, correspond to uncharacterized proteins indicating the presence of novel genes associated with drought tolerance in faba bean. The significance of stress-inducible signal transducers provides valuable information on the possible mechanisms underlying the faba bean response to drought stress, thus providing a foundation for future marker-assisted breeding in the crop.

Drought Tolerance

SNP genotyping

Medicago truncatula

Candidate gene

Genome-wide Association Study

10.3389/fpls.2023.1091875

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Asystasia gangetica (L.) T. Anders, subsp. micrantha (Nees) Ensermu (Acanthaceae), a new record for Venezuela.

Ernstia (2011)

Marianella Torres Lujan Natalia Gutiérrez Jair Gaviria

Acanthaceae

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Development of SCAR markers linked to a gene controlling absence of tannins in faba bean

Molecular Breeding (2006)

Natalia Gutiérrez C. M. Ávila Cristina Rodríguez‐Suárez M. T. Moreno A. M. Torres

Bulked segregant analysis

Tannin

10.1007/s11032-006-9063-9

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Citations (43)

Characterization and diagnostic marker for TTG1 regulating tannin and anthocyanin biosynthesis in faba bean

Scientific Reports (2019)

Natalia Gutiérrez A. M. Torres

Abstract Condensed tannins, found in coloured-flowering varieties of faba bean ( Vicia faba L) are, after vicine and convicine, one of the major anti-nutritional factors for monogastric animals. The development of tannin-free cultivars is a key goal in breeding to broaden the use of this legume in the animal feed industry. Two recessive genes, zt-1 and zt-2 , control the zero-tannin content and promote white-flowered plants. Previous studies exploiting synteny with the model Medicago truncatula reported a mutation in TTG1 , a gene encoding a WD40 transcription factor located in chromosome II, as the responsible for the zt-1 phenotypes. Here a comprehensive analysis of VfTTG1 (including phylogenetic relationships, gene structure and gene expression) has been conducted to confirm the identity of the gene and to reveal structural changes that may result in different functional alleles. The results confirmed the identity of the candidate and revealed the existence of two different alleles responsible for the phenotype: ttg1-a , probably due to a mutation in the promoter region, and ttg1-b caused by a deletion at the 5′end of VfTTG1 . Based on the sequencing results, an allele-specific diagnostic marker was designed that differentiate zt-1 from wild and zt-2 genotypes and facilitates its deployment in faba bean breeding programs.

Medicago truncatula

10.1038/s41598-019-52575-x

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Citations (29)

Potential MLO homologous genes in legume crops

Nicolás Rispail Nuno Felipe Almeida Natalia Gutiérrez A. M. Torres María Carlota Vaz Patto

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A bHLH transcription factor (VfTT8) underlieszt2, the locus determining zero tannin content in faba bean (Vicia faba L.)

bioRxiv (Cold Spring Harbor Laboratory) (2020)

Natalia Gutiérrez C. M. Ávila A. M. Torres

Abstract Faba bean ( Vicia faba L.) is an important protein-rich fodder crop widely cultivated in temperate areas. However, antinutritional compounds such as condensed tannins, limit the use of this protein source in monogastric feed formulations. Previous studies demonstrated that two recessive and complementary genes, zt1 and zt2 , control the absence of tannin and the white flower colour in faba bean. An ortholog of the Medicago WD40 transcription factor, ( TTG1 ) was reported to encode the zt1 phenotypes but the responsible gene for zt2 is still unknown. A candidate gene approach combined with linkage mapping, comparative genomics and gene expression has been used in this study to fine map the zt2 genomic region and to identify the regulatory gene controlling both traits. Seventy-two genes, including 23 regulatory genes (MYB and bHLH) predicted to be associated with anthocyanin expression together with WRKY proteins were screened and genotyped in three mapping populations. The linkage groups constructed identified the regulatory gene, TRANSPARENT TESTA8 ( TT8 ), encoding a basic helix-loop-helix transcription factor (bHLH), as the best candidate for zt2 . This finding was supported by qPCR analyses and further validated in different genetic backgrounds. Accordingly, VfTT8 was down-regulated in white flowered types, in contrast to the levels of expression in wild genotypes. Our results provide new insights on the regulatory mechanisms for tannin biosynthesis in faba bean and will favour the development of an ultimate zt2 diagnostic marker for the fast generation of new value-added cultivars free of tannins and improved nutritional value.

WRKY protein domain

MYB

Candidate gene

Medicago truncatula

10.1101/2020.03.06.980425

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A reference consensus genetic map for molecular markers and economically important traits in faba bean (Vicia fabaL.)

BMC Genomics (2013)

Zlatko Šatović C. M. Ávila Serafín Cruz-Izquierdo Ramón Díaz-Ruiz Gloria María García-Ruiz

Faba bean (Vicia faba L.) is among the earliest domesticated crops from the Near East. Today this legume is a key protein feed and food worldwide and continues to serve an important role in culinary traditions throughout Middle East, Mediterranean region, China and Ethiopia. Adapted to a wide range of soil types, the main faba bean breeding objectives are to improve yield, resistance to biotic and abiotic stresses, seed quality and other agronomic traits. Genomic approaches aimed at enhancing faba bean breeding programs require high-quality genetic linkage maps to facilitate quantitative trait locus analysis and gene tagging for use in a marker-assisted selection. The objective of this study was to construct a reference consensus map in faba bean by joining the information from the most relevant maps reported so far in this crop. A combination of two approaches, increasing the number of anchor loci in diverse mapping populations and joining the corresponding genetic maps, was used to develop a reference consensus map in faba bean. The map was constructed from three main recombinant inbreed populations derived from four parental lines, incorporates 729 markers and is based on 69 common loci. It spans 4,602 cM with a range from 323 to 1041 loci in six main linkage groups or chromosomes, and an average marker density of one locus every 6 cM. Locus order is generally well maintained between the consensus map and the individual maps. We have constructed a reliable and fairly dense consensus genetic linkage map that will serve as a basis for genomic approaches in faba bean research and breeding. The core map contains a larger number of markers than any previous individual map, covers existing gaps and achieves a wider coverage of the large faba bean genome as a whole. This tool can be used as a reference resource for studies in different genetic backgrounds, and provides a framework for transferring genetic information when using different marker technologies. Combined with syntenic approaches, the consensus map will increase marker density in selected genomic regions and will be useful for future faba bean molecular breeding applications.

Plant Breeding

10.1186/1471-2164-14-932

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Citations (62)

Additional file 2 of QTL dissection and mining of candidate genes for Ascochyta fabae and Orobanche crenata resistance in faba bean (Vicia faba L.)

Figshare (2021)

Natalia Gutiérrez A. M. Torres

Additional file 2. List of candidate genes assayed to saturate QTLs regions in the Vf6 x 136 RIL population detailing the genotyping method used, ID in the faba bean transcriptome (Ocaña et al. 2015), homologous gene in Medicago truncatula, protein function, chromosome location, genotyping results in cross 29H x 136 and common markers between the two RILs populations.

10.6084/m9.figshare.17065989

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Madrid: New Proximities in the Productive Post- Industrial City

De Gruyter eBooks (2024)

Julio de la Fuente Natalia Gutiérrez

10.1515/9783986120641-010

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