G. P. Georgiev

Institute of Gene Biology

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i-10 index

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А. П. Рысков

Institute of Gene Biology

O. P. Samarina

Ural State Medical University

Yuri V. Khramtsov

Institute of Gene Biology

Alexander S. Sobolev

Lomonosov Moscow State University

Seryozha Gontarev

Saints Cyril and Methodius University of Skopje

A. V. Ulasov

Institute of Gene Biology

Alexander Varshavsky

California Institute of Technology

T. N. Lupanova

Institute of Gene Biology

E. M. Lukanidin

Danish Cancer Society

Yu. V. Ilyin

Engelhardt Institute of Molecular Biology

Cooperative Institutions

Russian Academy of Sciences

114

Institute of Gene Biology

Institute of Molecular Genetics

Engelhardt Institute of Molecular Biology

Lomonosov Moscow State University

Institute of Bioorganic Chemistry

Ministry of Health of the Russian Federation

Saints Cyril and Methodius University of Skopje

University of Montenegro

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MODULAR NANOTRANSPORTERS CAPABLE OF BINDING WITH SARS-COV-2 VIRUS NUCLEOCAPSID PROTEIN INTO TARGET CELLS

Доклады Российской академии наук Науки о жизни (2023)

Yuri V. Khramtsov A. V. Ulasov T. N. Lupanova G. P. Georgiev Alexander S. Sobolev

Based on the literature data, an antibody-like molecule, a monobody, was selected that is capable of interacting with the nucleocapsid protein (N-protein) of the SARS-CoV-2 virus with high affinity (dissociation constant 6.7 nM). We have previously developed modular nanotransporters (MNTs) to deliver various molecules to a selected compartment of target cells. In this work, a monobody to the N-protein of the SARS-CoV-2 virus was included in the MNT using genetic engineering methods. In this MNT, a site for the cleavage of the monobody from the MNT in endosomes was also introduced. It was shown by thermophoresis that the cleavage of this monobody from MNT by the endosomal protease cathepsin B leads to a 12-fold increase in the affinity of the monobody for the N-protein. Cellular thermal shift assay showed the ability of the obtained MNT to interact with the N-protein in A431 cells transfected with the SARS-CoV-2 N-protein fused to the mRuby3 fluorescent protein.

Microscale Thermophoresis

Dissociation constant

Cleavage (geology)

10.31857/s2686738923700191

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Oncogene c-MYC Controls the Expression of PHF10 Subunit of PBAF Chromatin Remodeling Complex in SW620 Cell Line

Doklady Biochemistry and Biophysics (2019)

E. V. Tatarskiy G. P. Georgiev Н. В. Сошникова

Multiprotein complex

SWI/SNF

Oncogene Proteins

10.1134/s1607672919010204

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Citations (3)

DNA adjacent to attachment points of deoxyribonucleoprotein fibril to chromosomal axial structure is enriched in reiterated base sequences

Nucleic Acids Research (1978)

Sergey V. Razin V.L. Mantieva G. P. Georgiev

Mitotic chromosomes of L cells (metaphase plates) were dehistonized by centrifugation through a layer of 2 M NaCl and then treated with restriction endonuclease Bam HI. Alternatively, they were pretreated with EcoRI endonuclease, dehistonized, and additionally digested with EcoRI or HindIII. The DNA remaining attached to the axial structure of the chromosomes was isolated and investigated in renaturation experiments. It was found to be enriched in reiterated base sequences belonging to the satellite and to abundant intermediate repeats. The CsCl density gradient ultracentrifugation of this DNA separated the satellite from the fraction containing intermediate repeats.

Satellite DNA

BglII

10.1093/nar/5.12.4737

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Citations (34)

Waist-to-height ratio and body mass index as indicators of obesity in children from Montenegro

Nutrición Hospitalaria (2021)

Marija Bubanja Marina Vukotic G. P. Georgiev Zoran Milošević

Montenegro

Waist-to-height ratio

Body volume index

Body height

Body Shape Index

10.20960/nh.03687

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Heterogeneity of deoxynucleoprotein particles as evidenced by ultracentrifugation in cesium chloride density gradient

Journal of Molecular Biology (1969)

Yu. V. Ilyin G. P. Georgiev

Epigenome

Chromatin immunoprecipitation

Epigenomics

10.1016/0022-2836(69)90395-7

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Citations (62)

The properties of oligonucleotide fragments containing the triphosphorylated 5?-termini of nuclear pre-mRNA from Ehrlich ascites carcinoma cells

Molecular Biology Reports (1976)

G. Bajsz�r O. P. Samarina G. P. Georgiev

Ehrlich ascites carcinoma

10.1007/bf00366258

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Citations (3)

HSPBP1 in complex with the peptide of the innate immunity protein TAG7 is able to lyse tumor cells carrying TNFR1 receptor

Доклады Российской академии наук Науки о жизни (2024)

Е. А. Романова Daria M. Yurkina Denis V. Yashin Л. П. Сащенко G. P. Georgiev

The search for new cytotoxic agents capable of lysing tumor cells is an important task in the fight against cancer. Here we have shown that the protein HspBP1, the chaperone of the heat shock protein Hsp70, is able to form a complex with the previously discovered peptide (17.1) of the innate immunity protein Tag7. Experiments using thermophoresis have demonstrated that the affinity of the Tag7 protein peptide 17.1 to the HspBP1 molecule is 100 times higher than that of the full-sized Tag7 molecule. The addition of the 17.1-HspBP1 complex to tumor cells induces apoptosis and necroptosis in them. The results obtained in this work can be used to develop promising antitumor drugs.

10.31857/s2686738924010112

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International Journal of Applied Exercise Physiology (2020)

Velisa Vukasevic Izet Bajramović Marin Ćorluka Bojan Mašanović Zoran Milošević

The aim of this research is to analyze the scientific productivity of Montenegrin researchers in the field of sports sciences, as well as the trend of publishing in Montenegrin sports sciences journals. The research included studies focused on the sports sciences issues that were published in the period from 2002 to 2019. The electronic databases (Google Scholar, Scopus, and Web of Science) were searched for research articles available until July 22, 2019. Research findings were summarized in accordance with the PRISMA guidelines. They are presented the number of citations, h-index, i10-index as well as the number of documents by the authors were presented. The results of this study indicated that sports sciences researchers increased the number of publications from 2002 to 2019. The number of citations span from 299 and 5674 within most researchers, while the same researchers were cited quite less in Scopus and Web of Science databases. On the other hand, in the Google Scholar Database, there are three registered Montenegrin journals: The Sport Mont Journal is the most cited one with the highest h-index. The Montenegrin Journal of Sports Science and Medicine is the best ranked Montenegrin journal according to the bibliometric data that can be found in the Scopus and Web of Science databases, while the Journal of Anthropology of Sport and Physical Education is the lowest ranked but with significant progress in the last year.

Web of science

Impact factor

10.26655/ijaep.2020.1.8

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Methylation of DNA — One of the Major Epigenetic Markers

Biochemistry (Moscow) (2005)

С. В. Саложин Е. Б. Прохорчук G. P. Georgiev

Epigenomics

RNA-Directed DNA Methylation

Cancer Epigenetics

10.1007/s10541-005-0146-8

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Citations (53)

Oncogene c-MYC controls the expression of PHF10 subunit of PBAF chromatin remodeling complex in SW620 cell line

Доклады Академии наук (2019)

E. V. Tatarskiy G. P. Georgiev Н. В. Сошникова

The PBAF(SWI/SNF) multiprotein complex, which changes the chromatin structure, is widely involved in the regulation of eukaryotic gene expression. A specific component of this complex is the PHF10 protein, which is involved in recruiting this complex to chromatin. We showed that the PHF10 expression in cells of different lines is activated by the c-MYC oncogene. Since PHF10 stimulates cell proliferation, its c-MYC-dependent activation in cancer cells should lead to an increase in their proliferation rate.

SWI/SNF

Multiprotein complex

Oncogene Proteins

10.31857/s0869-56524845633-636

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