Juliana Campos Junqueira

Universidade Estadual Paulista (Unesp)

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Antônio Olavo Cardoso Jorge

Universidade Estadual Paulista (Unesp)

Rodnei Dennis Rossoni

Johnson & Johnson (Brazil)

Patrícia Pimentel de Barros

Universidade Federal do Rio Grande do Norte

Maíra Terra Garcia

Universidade Estadual Paulista (Unesp)

Luciane Dias de Oliveira

Universidade Estadual Paulista (Unesp)

Felipe de Camargo Ribeiro

Universidade Federal de São Paulo

Eleftherios Mylonakis

Methodist Hospital

Cristiane Aparecida Pereira

Universidade Estadual Paulista (Unesp)

Jéssica Diane dos Santos

Universidade Estadual Paulista (Unesp)

Anna Carolina Borges Pereira Costa

Concordia University

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Universidade Estadual Paulista (Unesp)

255

Universidade de São Paulo

Universidade de Taubaté

Universidade do Vale do Paraíba

Universidade Federal de São Paulo

Universidade Paulista

Institute of Science and Technology

National and Kapodistrian University of Athens

Prefeitura Municipal de Campos dos Goytacazes

Terasaki Foundation

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The biocompatibility and antifungal effect of Rosmarinus officinalis against Candida albicans in Galleria mellonella model

Scientific Reports (2022)

Vanessa Marques Meccatti Lívia Mara Alves Figueiredo-Godoi Thaís Cristine Pereira Patrícia Michelle Nagai de Lima Amjad Abu Hasna

This study was performed to evaluate the biocompatibility and antifungal effect of Rosmarinus officinalis against Candida albicans in Galleria mellonella model. Five different concentrations of R. officinalis glycolic extract (50; 25; 12.5 e 6.25 mg/mL) were used to evaluate its biocompatibility in G. mellonella model, in which the nystatin suspension (100; 50; 25; 12.5 e 6.25%) was used as a control group. The antifungal action of R. officinalis glycolic extract was evaluated on C. albicans for 72, 48 and 12 h at two different phases: (1) using the extract as therapeutic agent; and (2) using the extract as prophylactic agent. PBS was used as a negative control group. G. mellonella survival curves were plotted using the Kaplan-Meier method and statistical analysis was performed using the log-rank test (Mantel-Cox) and the significance level was set at (α ≤ 0.05). There was no significant difference among the groups in which all were biocompatible except of a significant death rate of 26.6% with nystatin 100%. In phase 1, it was found that after 7 days, there was no statistically significant difference among the prophylactic treatment groups. In phase 2, the groups of R. officinalis 6.25 mg/mL for 72 h and R. officinalis of 12.5 mg/mL for 24 h promoted the survival rate of the larvae in comparison with the control group with a significant difference (p = 0.017) and (p = 0.032) respectively. Therefore, R. officinalis extract is biocompatible in different concentrations and can be used as a prophylactic agent against fungal infection.

Rosmarinus

Galleria mellonella

Carnosic acid

Biocompatibility

10.1038/s41598-022-19425-9

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Citations (11)

Candida krusei and Candida glabrata reduce the filamentation of Candida albicans by downregulating expression of HWP1 gene

Folia Microbiologica (2017)

Patrícia Pimentel de Barros Fernanda Freire Rodnei Dennis Rossoni Juliana Campos Junqueira Antônio Olavo Cardoso Jorge

Candida glabrata

Candida krusei

Filamentation

10.1007/s12223-017-0500-4

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Citations (34)

Candida Biofilms

Frontiers research topics (2023)

Juliana Campos Junqueira Eleftherios Mylonakis

10.3389/978-2-8325-1356-9

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Citations (0)

Photodynamic inactivation of Staphylococcus aureus and Escherichia coli biofilms by malachite green and phenothiazine dyes: An in vitro study

Archives of Oral Biology (2011)

Simone Furgeri Godinho Vilela Juliana Campos Junqueira Júnia Oliveira Barbosa Marta Majewski Egberto Munin

Malachite green

Methylene blue

Toluidine

10.1016/j.archoralbio.2011.12.002

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Citations (100)

Comparison of the effect of rose bengal- and eosin Y-mediated photodynamic inactivation on planktonic cells and biofilms of Candida albicans

Lasers in Medical Science (2013)

Fernanda Freire Anna Carolina Borges Pereira Costa Cristiane Aparecida Pereira Milton Beltrame Juliana Campos Junqueira

Rose bengal

Eosin

10.1007/s10103-013-1435-x

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Citations (52)

Mixed biofilms formed by C. albicans and non-albicans species: a study of microbial interactions

Brazilian Oral Research (2016)

Jéssica Diane dos Santos Elisabete Piva Simone Furgeri Godinho Vilela Antônio Olavo Cardoso Jorge Juliana Campos Junqueira

Most Candida infections are related to microbial biofilms often formed by the association of different species. The objective of this study was to evaluate the interactions between Candida albicans and non-albicans species in biofilms formed in vitro. The non-albicans species studied were:Candida tropicalis, Candida glabrata andCandida krusei. Single and mixed biofilms (formed by clinical isolates of C. albicans and non-albicans species) were developed from standardized suspensions of each strain (107 cells/mL), on flat-bottom 96-well microtiter plates for 48 hour. These biofilms were analyzed by counting colony-forming units (CFU/mL) in Candida HiChrome agar and by determining cell viability, using the XTT 2,3-bis (2-methoxy-4-nitro-5-sulphophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide colorimetric assay. The results for both the CFU/mL count and the XTT colorimetric assay showed that all the species studied were capable of forming high levels of in vitro biofilm. The number of CFU/mL and the metabolic activity of C. albicans were reduced in mixed biofilms with non-albicans species, as compared with a singleC. albicans biofilm. Among the species tested, C. krusei exerted the highest inhibitory action against C. albicans. In conclusion, C. albicans established antagonistic interactions with non-albicans Candida species in mixed biofilms.

10.1590/1807-3107bor-2016.vol30.0023

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Citations (38)

Susceptibility of Candida albicans and Candida dubliniensis to erythrosine- and LED-mediated photodynamic therapy

Archives of Oral Biology (2011)

Anna Carolina Borges Pereira Costa Vanessa Maria de Campos Rasteiro Cristiane Aparecida Pereira Emily Setsuko Halter da Silva Hashimoto Milton Beltrame

The effect of erythrosine- and LED-mediated photodynamic therapy (PDT) on planktonic cultures and biofilms of Candida albicans and Candida dubliniensis was evaluated. Planktonic cultures of standardized suspensions (10(6)cells/mL) of C. albicans and C. dubliniensis were treated with erythrosine concentrations of 0.39-200 μM and LEDs in a 96-well microtiter plate. Biofilms formed by C. albicans and C. dubliniensis in the bottom of a 96-well microtiter plate were treated with 400 μM erythrosine and LEDs. After PDT, the biofilms were analysed by scanning electron microscopy (SEM). The antimicrobial effect of PDT against planktonic cultures and biofilms was verified by counting colony-forming units (CFU/mL), and the data were submitted to analysis of variance and the Tukey test (P<0.05). C. albicans and C. dubliniensis were not detectable after PDT of planktonic cultures with erythrosine concentrations of 3.12 μM or higher. The CFU/mL values obtained from biofilms were reduced 0.74 log(10) for C. albicans and 0.21 log(10) for C. dubliniensis. SEM revealed a decrease in the quantity of yeasts and hyphae in the biofilm after PDT. In conclusion, C. albicans and C. dubliniensis were susceptible to erythrosine- and LED-mediated PDT, but the biofilms of both Candida species were more resistant than their planktonic counterparts.

Erythrosine

Candida dubliniensis

10.1016/j.archoralbio.2011.05.013

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Citations (76)

Estudo microbiologico das maos e luvas dos graduandos de odontologia

Revista de odontologia da UNICID (2003)

Sibeli Bonafe Santos Juliana Campos Junqueira Célia Regina Gonçalves e Silva Ivan Balducci Antônio Olavo Cardoso Jorge

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Citations (0)

Susceptibility of Candida albicans and Candida dubliniensis planktonic cultures and biofilms to erythrosine- and LED-mediated photodynamic therapy