Mathias Köppen

Instituto Gulbenkian de Ciência

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Carl‐Philipp Heisenberg

Institute of Science and Technology Austria

Pablo Oteíza

Max Planck Institute for Ornithology

Miguel L. Concha

University of Chile

Eduardo Pulgar

Neuroscience Institute

Steffen Härtel

University of Chile

Lara Carvalho

Universidade Nova de Lisboa

Paul A. Sims

University of Oklahoma

Jeffrey D. Hardin

University of Wisconsin–Madison

Cristina Melo

University of Chile

Jeffrey S. Simske

Case Western Reserve University

Cooperative Institutions

University of Chile

Max Planck Institute of Molecular Cell Biology and Genetics

KU Leuven

Instituto de Neurociencia Biomédica

Howard Hughes Medical Institute

Nagoya University

Universidad del Desarrollo

Albert Einstein College of Medicine

University of Wisconsin–Madison

Vlaams Instituut voor Biotechnologie

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Cleavage and Gastrulation in Zebrafish Embryos

Encyclopedia of Life Sciences (2005)

Mathias Köppen Carl‐Philipp Heisenberg

Abstract During zebrafish gastrulation, the interplay between patterning events and morphogenesis creates an embryo out of a seemingly unstructured blastula stage embryo, an embryo with distinct polarities along its anterior–posterior, dorsoventral and left–right axes at the end of gastrulation.

Blastula

Cleavage (geology)

Polarity in embryogenesis

10.1038/npg.els.0001072

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Citations (0)

Cooperative regulation of AJM-1 controls junctional integrity in Caenorhabditis elegans epithelia

Nature Cell Biology (2001)

Mathias Köppen Jeffrey S. Simske Paul A. Sims Bonnie L. Firestein David H. Hall

Septate junctions

10.1038/ncb1101-983

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Citations (313)

S10-05 Linking organ formation to left–right patterning in the embryonic zebrafish

Mechanisms of Development (2009)

Pablo Oteíza Carmen G. Lemus Mathias Köppen Karina Palma Michael Krieg

10.1016/j.mod.2009.06.970

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Citations (1)

Coordinated cell-shape changes control epithelial movement in zebrafish andDrosophila

Development (2006)

Mathias Köppen Beatriz García Fernández Lara Carvalho António Jacinto Carl‐Philipp Heisenberg

Epithelial morphogenesis depends on coordinated changes in cell shape, a process that is still poorly understood. During zebrafish epiboly and Drosophila dorsal closure, cell-shape changes at the epithelial margin are of critical importance. Here evidence is provided for a conserved mechanism of local actin and myosin 2 recruitment during theses events. It was found that during epiboly of the zebrafish embryo, the movement of the outer epithelium (enveloping layer) over the yolk cell surface involves the constriction of marginal cells. This process depends on the recruitment of actin and myosin 2 within the yolk cytoplasm along the margin of the enveloping layer. Actin and myosin 2 recruitment within the yolk cytoplasm requires the Ste20-like kinase Msn1, an orthologue of Drosophila Misshapen. Similarly, in Drosophila, actin and myosin 2 localization and cell constriction at the margin of the epidermis mediate dorsal closure and are controlled by Misshapen. Thus, this study has characterized a conserved mechanism underlying coordinated cell-shape changes during epithelial morphogenesis.

Epiboly

Apical constriction

10.1242/dev.02439

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Citations (149)

Planar cell polarity signalling regulates cell adhesion properties in progenitors of the zebrafish laterality organ

Development (2010)

Pablo Oteíza Mathias Köppen Michael Krieg Eduardo Pulgar Cecilia Farias

Organ formation requires the precise assembly of progenitor cells into a functional multicellular structure. Mechanical forces probably participate in this process but how they influence organ morphogenesis is still unclear. Here, we show that Wnt11- and Prickle1a-mediated planar cell polarity (PCP) signalling coordinates the formation of the zebrafish ciliated laterality organ (Kupffer's vesicle) by regulating adhesion properties between organ progenitor cells (the dorsal forerunner cells, DFCs). Combined inhibition of Wnt11 and Prickle1a reduces DFC cell-cell adhesion and impairs their compaction and arrangement during vesicle lumen formation. This leads to the formation of a mis-shapen vesicle with small fragmented lumina and shortened cilia, resulting in severely impaired organ function and, as a consequence, randomised laterality of both molecular and visceral asymmetries. Our results reveal a novel role for PCP-dependent cell adhesion in coordinating the supracellular organisation of progenitor cells during vertebrate laterality organ formation.

Progenitor

10.1242/dev.049981

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Citations (67)

Lpp is involved in Wnt/PCP signaling and acts together with Scrib to mediate convergence and extension movements during zebrafish gastrulation

Developmental Biology (2008)

Hilke Vervenne Koen R.M.O. Crombez Kathleen Lambaerts Lara Carvalho Mathias Köppen

Convergent extension

Cell polarity

Morpholino

10.1016/j.ydbio.2008.05.529

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Citations (29)

Establishment of a tissue-specific RNAi system in C. elegans

Gene (2007)

Hiroshi Qadota Makiko Inoue Takao Hikita Mathias Köppen Jeffrey D. Hardin

10.1016/j.gene.2007.06.020

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Citations (200)

Origin and shaping of the laterality organ in zebrafish

Development (2008)

Pablo Oteíza Mathias Köppen Miguel L. Concha Carl‐Philipp Heisenberg

Handedness of the vertebrate body plan critically depends on transient embryonic structures/organs that generate cilia-dependent leftward fluid flow within constrained extracellular environments. Although the function of ciliated organs in laterality determination has been extensively studied, how they are formed during embryogenesis is still poorly understood. Here we show that Kupffer's vesicle (KV), the zebrafish organ of laterality, arises from a surface epithelium previously thought to adopt exclusively extra-embryonic fates. Live multi-photon confocal imaging reveals that surface epithelial cells undergo Nodal/TGFbeta signalling-dependent ingression at the dorsal germ ring margin prior to gastrulation, to give rise to dorsal forerunner cells (DFCs), the precursors of KV. DFCs then migrate attached to the overlying surface epithelium and rearrange into rosette-like epithelial structures at the end of gastrulation. During early somitogenesis, these epithelial rosettes coalesce into a single rosette that differentiates into the KV with a ciliated lumen at its apical centre. Our results provide novel insights into the morphogenetic transformations that shape the laterality organ in zebrafish and suggest a conserved progenitor role of the surface epithelium during laterality organ formation in vertebrates.

Ciliogenesis

Fate mapping

10.1242/dev.022228

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Citations (121)

The cell junction protein VAB-9 regulates adhesion and epidermal morphology in C. elegans

Nature Cell Biology (2003)

Jeffrey S. Simske Mathias Köppen Paul A. Sims Jonathan Hodgkin Alicia Yonkof

Adherens junction

Cell polarity

Epithelial polarity

Vinculin

Septate junctions

10.1038/ncb1002

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Citations (91)