Wael N. Hozzein

Beni-Suef University

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Wen‐Jun Li

Sun Yat-sen University

Dalal Hussien M. Alkhalifah

Princess Nourah bint Abdulrahman University

Mohammed A. M. Wadaan

King Saud University

Min Xiao

Fudan University

Hamada AbdElgawad

University of Antwerp

Samy Selim

Al Jouf University

Nimaichand Salam

Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai)

Areej A. Al-Khalaf

Princess Nourah bint Abdulrahman University

Li Li

Qingdao National Laboratory for Marine Science and Technology

Hongfei Wang

Shanxi Provincial Cancer Hospital

Cooperative Institutions

Chinese Academy of Sciences

Beni-Suef University

King Saud University

Sun Yat-sen University

Yunnan University

Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)

University of Chinese Academy of Sciences

Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai)

Fudan University

Zagazig University

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Nocardiopsis alkaliphila sp. nov., a novel alkaliphilic actinomycete isolated from desert soil in Egypt

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY (2004)

Wael N. Hozzein Wen‐Jun Li Mohammed I. Ali Ola Hammouda Sahar M. Mousa

An alkaliphilic actinomycete strain, designated YIM 80379 T , was isolated from a soil sample collected from the eastern desert of Egypt and subjected to polyphasic taxonomy. The strain produced substrate and aerial mycelia on different media, with an optimum pH for growth of 9·5–10 and scarce or no growth at pH 7. Strain YIM 80379 T contained meso -diaminopimelic acid, no diagnostic sugars, type PIII phospholipids and MK-10(H 6 ) and MK-10(H 8 ) as the predominant menaquinones. All of these characters assign isolate YIM 80379 T consistently to the genus Nocardiopsis . This was confirmed by 16S rDNA analysis. It can be differentiated from all Nocardiopsis species with validly published names by phenotypic characteristics, phylogenetic analysis and DNA–DNA hybridization results. On the basis of polyphasic evidence, a novel species, Nocardiopsis alkaliphila sp. nov., is proposed. The type strain of the species is YIM 80379 T (=CCTCC AA001031 T =DSM 44657 T ).

Strain (injury)

Nocardiaceae

Mycology

10.1099/ijs.0.02832-0

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Citations (70)

In Vitro Cytotoxicity and Hemolysis Effect of Poly-Gamma-Glutamic Acid Nano-Polymer Biosynthesized Using Some Isolates of Bacillus spp.

Microbiology Research (2023)

Eman ElSayed Ahmed A. Farghali Mohamed I. Zanaty Medhat Abdel-Fattah Dalal Hussien M. Alkhalifah

Natural polymers have good biological, biocompatible properties and a low degradation rate, so they can be used in medical applications. This study demonstrates an optimized biosynthesis for poly-gamma-glutamic acid nano-polymer (ɣ-PGA NP) using three bacterial isolates encoded as B4, B5, and B6. The 16S rRNA gene sequence of the isolates showed 98.5, 99, and 99.8% similarity with Bacillus sp., with accession numbers MZ976778, MZ976779, and MZ956153, respectively. The optimal conditions of the biosynthesis were 35 °C, followed by incubation for 30 h, 7 pH, 0.7 O.D600 of bacterial broth, carbon sources of glutamic acid and glucose, and ammonium sulfate as the nitrogen source. Biosynthesized ɣ-PGA NPs were characterized using X-ray diffraction (XRD), Zeta sizer, Fourier transform infrared (FT-IR), transmission electron microscopy (TEM), and nuclear magnetic resonance (1HNMR). The TEM study showed the formation of oval-shaped ɣ-PGA NPs with sizes 31.75–32.69 nm, 18.63–21.66 nm, and 37.57–39.22 nm for B4, B5, and B6, respectively. XRD, FT-IR, and 1HNMR confirmed the purity of the polymer. The hemolysis rates of the biosynthesized ɣ-PGA NPs were below the acceptable value. Moreover, the cytotoxicity test conducted using an MTT assay on peripheral blood mononuclear cells (PBMCs) revealed no significant effect on the cell viability within a dose range of up to 512 μg/mL.

Zeta potential

Glutamic acid

MTT assay

Ammonium chloride

10.3390/microbiolres14040118

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Citations (2)

Microbial Diversity in Asian Deserts: Distribution, Biotechnological Importance, and Environmental Impacts

Microorganisms for sustainability (2018)

Nimaichand Salam Ziwen Yang Mipeshwaree Devi Asem Wael N. Hozzein Wen‐Jun Li

10.1007/978-981-13-0329-6_13

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Citations (1)

Bacteriophage-mediated biosynthesis of MnO2NPs and MgONPs and their role in the protection of plants from bacterial pathogens

Frontiers in Microbiology (2023)

Solabomi Olaitan Ogunyemi Yasmine Abdallah Ezzeldin Ibrahim Yang Zhang Jian Bi

Xanthomonas oryzae pv. oryzae (Xoo) is the plant pathogen of Bacterial Leaf Blight (BLB), which causes yield loss in rice.In this study, the lysate of Xoo bacteriophage X3 was used to mediate the bio-synthesis of MgO and MnO2. The physiochemical features of MgONPs and MnO2NPs were observed via Ultraviolet - Visible spectroscopy (UV-Vis), X-ray diffraction (XRD), Transmission/Scanning electron microscopy (TEM/SEM), Energy dispersive spectrum (EDS), and Fourier-transform infrared spectrum (FTIR). The impact of nanoparticles on plant growth and bacterial leaf blight disease were evaluated. Chlorophyll fluorescence was used to determine whether the nanoparticles application were toxic to the plants.An absorption peak of 215 and 230 nm for MgO and MnO2, respectively, confirmed nanoparticle formation via UV-Vis. The crystalline nature of the nanoparticles was detected by the analysis of XRD. Bacteriological tests indicated that MgONPs and MnO2NPs sized 12.5 and 9.8 nm, respectively, had strong in vitro antibacterial effects on rice bacterial blight pathogen, Xoo. MnO2NPs were found to have the most significant antagonist effect on nutrient agar plates, while MgONPs had the most significant impact on bacterial growth in nutrient broth and on cellular efflux. Furthermore, no toxicity to plants was observed for MgONPs and MnO2NPs, indeed, MgONPs at 200 μg/mL significantly increased the quantum efficiency of PSII photochemistry on the model plant, Arabidopsis, in light (ΦPSII) compared to other interactions. Additionally, significant suppression of BLB was noted in rice seedlings amended with the synthesized MgONPs and MnO2NPs. MnO2NPs showed promotion of plant growth in the presence of Xoo compared to MgONPs.An effective alternative for the biological production of MgONPs and MnO2NPs was reported, which serves as an effective substitute to control plant bacterial disease with no phytotoxic effect.

Nutrient agar

Ralstonia solanacearum

10.3389/fmicb.2023.1193206

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Citations (10)

Georgenia alba sp. nov., a novel halotolerant actinobacterium isolated from a desert sand sample

Antonie van Leeuwenhoek (2018)

Lanyu Li Ziwen Yang Mipeshwaree Devi Asem Nimaichand Salam Min Xiao

Strain (injury)

Halotolerance

Rhamnose

10.1007/s10482-018-1145-2

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Citations (14)

Topical Application of Propolis Enhances Cutaneous Wound Healing by Promoting TGF-Beta/Smad-Mediated Collagen Production in a Streptozotocin-Induced Type I Diabetic Mouse Model

Cellular Physiology and Biochemistry (2015)

Wael N. Hozzein Gamal Badr Ahmad A. Al Ghamdi Ayat A. Sayed Noori S. Al‐Waili

Background/Aims: Impaired wound healing is considered to be one of the most serious complications associated with diabetes as it significantly increases the susceptibility of patients to infection. Propolis is a natural bee product used extensively in foods and beverages that has significant benefits to human health. In particular, propolis has antioxidant, anti-inflammatory and analgesic effects that could be useful for improving wound healing. In this study, we investigated the effects of topical application of propolis on the healing and closure of diabetic wounds in a streptozotocin (STZ)-induced type I diabetic mouse model. Methods: Sixty male mice were distributed equally into 3 experimental groups: group 1, non-diabetic control mice; group 2, diabetic mice; and group 3, diabetic mice treated daily with a topical application of propolis. Results: We found that diabetic mice exhibited delayed wound closure characterized by a significant decrease in the levels of TGF-β1 and a prolonged elevation of the levels of inflammatory cytokines (IL-1β, IL-6 and TNF-α) and MMP9 in wound tissues compared with control non-diabetic mice. Moreover, the wound tissues of diabetic mice showed a marked reduction in the phosphorylation of Smad2 and Smad3 as well as a marked reduction in collagen production. Interestingly, compared with untreated diabetic mice, topical application of propolis significantly enhanced the closure of diabetic wounds and decreased the levels of IL-1β, IL-6, TNF-α and MMP9 to near normal levels. Most importantly, compared with untreated diabetic mice, the treatment of diabetic mice with propolis significantly enhanced the production of collagen via the TGF-β1/Smad2,3 signaling axis in wounded tissues. Conclusion: Our findings reveal the molecular mechanisms underlying the improved healing and closure of diabetic wounds following topical propolis application.

Propolis

MMP9

10.1159/000430221

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Citations (126)

RETRACTED ARTICLE: Oral supplementation of diabetic mice with propolis restores the proliferation capacity and chemotaxis of B and T lymphocytes towards CCL21 and CXCL12 by modulating the lipid profile, the pro-inflammatory cytokine levels and oxidative stress

BMC Immunology (2015)

Ahmad A. Al Ghamdi Gamal Badr Wael N. Hozzein Ahmed A. Allam Noori S. Al‐Waili

Type 1 diabetes mellitus (T1D) is a chronic autoimmune disease caused by the selective destruction of pancreatic β cells, followed by hyperglycemia, oxidative stress and the subsequent extensive impairment of immune cell functions, a phenomenon responsible for the development of chronic diabetic complications. Propolis, a natural bee product that is extensively used in foods and beverages, significantly benefits human health. Specifically, propolis exerts antioxidant, anti-inflammatory and analgesic effects that may improve diabetic complications. To further elucidate the potential benefits of propolis, the present study investigated the effect of dietary supplementation with propolis on the plasma cytokine profiles, free radical levels, lipid profile and lymphocyte proliferation and chemotaxis in a streptozotocin (STZ)-induced type I diabetic mouse model.Thirty male mice were equally distributed into 3 experimental groups: group 1, non-diabetic control mice; group 2, diabetic mice; and group 3, diabetic mice supplemented daily with an ethanol-soluble derivative of propolis (100 mg/kg body weight) for 1 month.First, the induction of diabetes in mice was associated with hyperglycemia and significant decreases in the insulin level and the lymphocyte count. In this context, diabetic mice exhibited severe diabetic complications, as demonstrated by a significant decrease in the levels of IL-2, IL-4 and IL-7, prolonged elevation of the levels of pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α) and reactive oxygen species (ROS) and altered lipid profiles compared with control non-diabetic mice. Moreover, antigen stimulation of B and T lymphocytes markedly reduced the proliferative capacity and chemotaxis of these cells towards CCL21 and CXCL12 in diabetic mice compared with control mice. Interestingly, compared with diabetes induction alone, treatment of diabetic mice with propolis significantly restored the plasma cytokine and ROS levels and the lipid profile to nearly normal levels. Most importantly, compared with untreated diabetic mice, diabetic mice treated with propolis exhibited significantly enhanced lymphocyte proliferation and chemotaxis towards CCL21 and CXCL12.Our findings reveal the potential immuno-modulatory effects of propolis, which acts as a natural antioxidant to enhance the function of immune cells during diabetes.

Propolis

Proinflammatory cytokine

Lipid Profile

10.1186/s12865-015-0117-9

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Citations (45)

Antioxidant and Antibacterial Activities of Silver Nanoparticles Biosynthesized by Moringa oleifera through Response Surface Methodology

Journal of Nanomaterials (2022)

A. B. Abeer Mohammed Amr M. Mohamed Noura El‐Ahmady El‐Naggar Hoda Mahrous Ghada M. Nasr

The research highlights the environmentally sustainable biosynthesis of silver nanoparticles from fresh leaves of the herbal medicinal plant Moringa oleifera . They may have been used as anti‐inflammatory, anticancer, and antimicrobial agents. M. oleifera extract both reduces and stabilizes silver nanoparticles (AgNPs). Optimum factors needed for AgNP biosynthesis were studied using a central composite design (CCD) matrix. Ultraviolet‐visible (UV–Vis) absorption spectroscopy, transmission electron microscopy, and Fourier‐transform infrared spectroscopy were used to confirm and characterize the synthesized AgNPs. The biogenic AgNPs demonstrated substantial antibacterial potential against the pathogenic strains Escherichia coli , Klebsiella pneumoniae , Staphylococcus aureus , and Bacillus subtilis . The antioxidant activity of biosynthesized AgNPs with M. oleifera extract increased from 11.96% when the concentration of the extract was 4 mg/mL to 63.79% at a plant concentration of 20 mg/mL. This research provides an easy and cost‐effective technique for the production of stable nanoparticles, with an evaluation of their bioactivity.

Moringa

Silver nanoparticle

10.1155/2022/9984308

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Citations (39)

Actinomycetes Enrich Soil Rhizosphere and Improve Seed Quality as well as Productivity of Legumes by Boosting Nitrogen Availability and Metabolism

Biomolecules (2020)

Hamada AbdElgawad Walid Abuelsoud Mahmoud M. Y. Madany Samy Selim Gaurav Zinta

The use of actinomycetes for improving soil fertility and plant production is an attractive strategy for developing sustainable agricultural systems due to their effectiveness, eco-friendliness, and low production cost. Out of 17 species isolated from the soil rhizosphere of legume crops, 4 bioactive isolates were selected and their impact on 5 legumes: soybean, kidney bean, chickpea, lentil, and pea were evaluated. According to the morphological and molecular identification, these isolates belong to the genus Streptomyces. Here, we showed that these isolates increased soil nutrients and organic matter content and improved soil microbial populations. At the plant level, soil enrichment with actinomycetes increased photosynthetic reactions and eventually increased legume yield. Actinomycetes also increased nitrogen availability in soil and legume tissue and seeds, which induced the activity of key nitrogen metabolizing enzymes, e.g., glutamine synthetase, glutamate synthase, and nitrate reductase. In addition to increased nitrogen-containing amino acids levels, we also report high sugar, organic acids, and fatty acids as well as antioxidant phenolics, mineral, and vitamins levels in actinomycete treated legume seeds, which in turn improved their seed quality. Overall, this study shed the light on the impact of actinomycetes on enhancing the quality and productivity of legume crops by boosting the bioactive primary and secondary metabolites. Moreover, our findings emphasize the positive role of actinomycetes in improving the soil by enriching its microbial population. Therefore, our data reinforce the usage of actinomycetes as biofertilizers to provide sustainable food production and achieve biosafety.

Biofertilizer

10.3390/biom10121675

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Citations (88)

Biogeography of Nocardiopsis strains from hypersaline environments of Yunnan and Xinjiang Provinces, western China

Scientific Reports (2015)

Songtao He Xiao‐Yang Zhi Hongchen Jiang Ling‐Ling Yang Jinyuan Wu

Abstract The genus Nocardiopsis is a widespread group within the phylum Actinobacteria and has been isolated from various salty environments worldwide. However, little is known about whether biogeography affects Nocardiopsis distribution in various hypersaline environments. Such information is essential for understanding the ecology of Nocardiopsis . Here we analyzed 16S rRNA, gyrB , rpoB and sodA genes of 78 Nocardiopsis strains isolated from hypersaline environments in Yunnan and Xinjiang Provinces of western China. The obtained Nocardiopsis strains were classified into five operational taxonomic units, each comprising location-specific phylo- and genotypes. Statistical analyses showed that spatial distance and environmental factors substantially influenced Nocardiopsis distribution in hypersaline environments: the former had stronger influence at large spatial scales, whereas the latter was more influential at small spatial scales.

Extreme environment

10.1038/srep13323

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Citations (11)