<p>This figure shows that the amount of time to achieve first Cp {less than or equal to} 17 mg/dL was significantly reduced for TN compared to non-TN patients.</p>
Abstract Background: Treatment with trastuzumab prolongs overall survival when given to patients (pts) with Her2/neu+ breast cancer (BC). The primary toxicity of trastuzumab is cardiotoxicity and the incidence is estimated at 2-4% in the adjuvant setting. The mechanism for trastuzumab-induced cardiotoxicity is not known. Although Her2neu expression is usually not seen on cardiac myocytes, its expression has been shown to be upregulated after chemotherapy. Trastuzumab is a monoclonal antibody that binds to the extracellular domain of Her2/neu. We hypothesized that single nucleotide polymorphisms (SNPs) in the Her2/neu receptor may play a role in trastuzumab associated cardiotoxicity. Methods: 140 pts with BC who were treated with chemotherapy and trastuzumab were enrolled into an IRB approved protocol at the Weill Cornell Medical College between July 2008 and March 2013. Cardiotoxicity was defined as either symptomatic CHF, or a decline in LVEF of 15% (or if LVEF <55% a decline in LVEF of 10%) that required management with medications and led to temporary or permanent discontinuation of trastuzumab. 11 nonsynonomous human ErbB2 SNPs were identified in the National Center for Biotechnology Information SNP database (rs1136201, rs2172826, rs28933368, rs28933369, rs28933370, rs34602395, rs36085723, rs4252633, rs55943169, rs56366519, rs61552325). Genotyping of SNPs was performed on DNA prepared from blood or buccal washes. The relationship between SNP characteristics and cardiotoxicity status was assessed by the chi-square test and multivariable logistic regression analysis. Results: 140 subjects (29 with cardiotoxicity and 111 without) had 11 SNPs sequenced. Median age of subjects was 56 years (range: 32-85), mean baseline LVEF was 65% (±6%). 16.4% of subjects had hypertension (HTN). 80% of patients were Caucasian, 10% East Asian, 7.1% African American, 2.9% South Asian. There were two SNPs for which there was variation seen among subjects: rs 1136201 (corresponding to codon 655) and rs61552325 (codon 1170). The frequencies of the codon 655 polymorphisms were: AA (Ile/Ile) 67.9%, AG (Ile/Val) 29.3%, and GG (Val/Val) 2.9%. The frequencies of the codon 1170 polymorphisms were: CC (Pro/Pro) 20.7%, GC (Ala/Pro) 45.7%, and GG (Ala/Ala) 33.6%. There was no association observed between the codon 655 polymorphism and cardiotoxicity (p = 0.96). A significant association between cardiotoxicity and the codon 1170 polymorphism was observed, with subjects having cardiotoxicity being more likely to carry the CC allele compared with subjects without cardiotoxicity (34.5% vs 17.1%, p = 0.04). This association persisted after multivariable adjustment for age, race, and HTN status (adjusted OR = 2.60, 95% CI = 1.02-6.62, p = 0.046). Conclusion: In this study, the Her2/neu 1170 Pro/Pro polymorphism was associated with trastuzumab cardiotoxicity. If confirmed in a larger series, this polymorphism could be used to identify pts who may be at increased risk for cardiotoxicity and who may benefit from treatments associated with less cardiotoxicity. Furthermore, the Her2/neu 1170 SNP has previously been implicated as a minor histocompatibility antigen, and our findings raise the possibility that immune mediated mechanisms may play a role in trastuzumab related cardiotoxicity. Citation Information: Cancer Res 2013;73(24 Suppl): Abstract nr P6-05-06.
<p>This figure shows that the amount of time to achieve first Cp {less than or equal to} 17 mg/dL was significantly reduced for TN compared to non-TN patients.</p>
A patient with syndromic Duane retraction syndrome harbors a chromosome 811.1q13.2 inversion and 8p11.1-q12.3 marker chromosome containing subregions with differing mosaicism and allele frequencies. This case highlights the potential requirement for multiple genetic methods to gain insight into genotype–phenotype correlation, and ultimately into molecular mechanisms that underlie human disease. Duane retraction syndrome (DRS) occurs in approximately 1 in 1000 individuals and most commonly manifests as limited abduction with globe retraction on attempted adduction. It is believed to result from errors in the development of the abducens nucleus or nerve, and aberrant innervation of the lateral rectus muscle by axons of the oculomotor nerve. While dominant DRS pedigrees can harbor mutations in alpha-chimerin (CHN1) 1 or Sal-like protein 4 (SALL4), 2, 3 most cases of DRS are simplex and genetically undefined. For almost two decades, rare patients with simplex, syndromic DRS have been reported to harbor cytogenetic abnormalities in the chromosomal region 8q12-8q13 that define the DURS1 locus, as summarized below and in Figure 1. The initial three patients that defined the DURS1 locus harbored a deletion or had a translocation breakpoint at 8q13. The first patient had DRS, branchiootorenal syndrome, hydrocephalus, trapezius muscle aplasia, and a large de novo interstitial deletion del(8)(q13.1-q21.11) originating on the paternal allele 4. The second patient had bilateral DRS type 1, severe intellectual disabilities, microcephaly, dysmorphisms, brachydactyly and left club foot, and harbored an insertion of 8q11.2-q13 into 6q25 with a deletion, del(8)(q12.3q13.2) 5. The third patient had DRS, dysgenetic gonads, hypoplastic external genitalia and glandular hypospadias, and a de novo reciprocal translocation t(6;8)(q26;q13) with the chromosome 8q13 translocation breakpoint located within intron 1 of carboxypeptidase A6 (CPA6) 6, 7. Notably, a fourth patient with branchiootorenal syndrome but not DRS is reported to harbor a deletion from distal 8q13.1 through 8q21.13 8. Recently, three patients with DRS were reported to harbor 8q12 microduplications 9-11. Their phenotypes included DRS, sensorineural deafness, intellectual disabilities, hypotonia, dysmorphisms, and congenital heart and kidney defects 9-11. The three patients share a 1.2 Mb duplicated region encompassing carbonic anhydrase VIII (CA8), RAS-associated protein RAB2 (RAB2A), chromodomain helicase DNA binding protein 7 (CHD7), and clavesin 1 (CLVS1) (Fig. 1). A fourth patient harboring a 2.7 Mb 8q12 microduplication also encompassing these four genes had dysmorphic features, congenital heart defect, and torticollis, but did not exhibit DRS 12. In this study, we describe a boy with syndromic DRS and complex structural variations involving both 8q12 and 8q13. The proband and his maternal grandmother participated in an ongoing genetic study of DRS at Boston Children's Hospital, and provided written informed consent to a protocol conforming to the Declaration of Helsinki and approved by Boston Children's Hospital institutional review board. The parents of the proband were not available for participation. Medical and ophthalmologic history and physical examination findings were obtained from medical records. Both participants provided a blood sample for DNA extraction, and the proband also provided a sample for cell line generation. Epstein-Barr virus transformation was performed by the Biosample Services Facility at Partners Center for Personalized Genetic Medicine, Cambridge MA, for initiation of a lymphoblastoid cell line. Bacterial artificial chromosome (BAC) clones were selected using University of California, Santa Cruz (UCSC) genome browser (http://genome.ucsc.edu; hg19) and obtained from Children's Hospital Oakland Research Institute (CHORI, Oakland, CA). BAC DNA was isolated using standard protocols and labeled directly with either SpectrumGreen- or SpectrumOrange-conjugated dUTP following the manufacturer's instructions (Nick Translation Kit, catalog no.: 32-801300; Abbott Laboratories. IL). Cot-I DNA (10 μL) was added for every 1 μg of labeled probe to suppress repetitive sequences, and probes were ethanol precipitated and resuspended in 50% Hybrisol (50% formamide, 2×saline-sodium citrate [SSC], 10% dextran sulfate) (Abbott Laboratories). Metaphase chromosomes were prepared using standard cytogenetic protocols 13. Fluorescence in situ hybridization (FISH) was performed with direct-labeled BAC probes to map each inversion breakpoint. Probes were hybridized in differentially labeled pairs (SpectrumGreen and SpectrumOrange [Vysis, Abbott Laboratories]). The telomeric inversion breakpoint was mapped using BAC clones RP11-89A16 (8q12.3-8q13.1), RP11-282D10 (8q13.1), RP11-212P10 (8q13.1), RP11-271O1 (8q13.1), RP11-343B22 (8q13.2), and RP11-131P18 (8q13.2), and refined using 8q13.2 BAC clones RP11-396J6, RP11-566L6, RP11-664D7, 349K17, RP11-159C14, RP11-50A22, RP11-779P1, and RP11-939K17. The centromeric inversion breakpoint was mapped using RP11-726G23 (8p11.21-8p11.1), RP11-8790P20 (8q11.21), RP11-598P20 (8p11.21), RP11-1031I13 (8q11.1), and 1102L10 and 1130I3 (8q11.21). Probes and chromosomes were codenatured at 72°C for 2 min and hybridized overnight at 37°C in a HYBrite apparatus (Abbott Molecular/Vysis). Slides were washed in 50% formamide/2×SSC at 37°C for 20 min and 2×SSC at 37°C for 20 min. 4′,6′-diamidino-2-phenylindole hydrochloride (DAPI) was used as counterstain. Hybridization results were assessed with a Zeiss Axioskop 2 epifluorescence microscope (Thornwood, NY) or an Olympus BX51 microscope (Center Valley, PA), and images were acquired with an Applied Imaging CytoVision cytogenetics workstation (Santa Clara, CA). A minimum of 10 metaphases was scored per hybridization. Chromosomal microarray analysis (CMA) studies were performed to detect copy number variation (CNV) using two different platforms. A custom high-resolution microarray was designed to target the DURS1 region (hg19; chr8: 41,880,843-74,837,446). Overlapping probes of 50–60 bases in length were tiled across the DURS1 region beginning every ~10 bases (8p11.21-8q13.3) (Roche Nimblegen, Madison, WI). The experiment was performed twice, using standard dye swap. An Illumina HumanOmniExpress BeadChip array composed of ~730 K single nucleotide polymorphisms (SNPs) (Illumina, San Diego, CA) was performed following the manufacturer's directions. Data were evaluated and analyzed using Illumina's GenomeStudio v2011.1 and Nexus CN 7.0 Standard Edition software (updated on 19 April 2012). The Nexus analysis settings used for reporting CNV(s) were as follows: SNP-FASST2 segmentation; significance threshold = 1.0E−9; max contiguous probe spacing (Kbp) = 1000.0; min number of probes per segment = 15; Log-R thresholds were high gain = 0.41; gain = 0.13; loss = −0.23; big loss = −1.1; sex chromosome gain (3:1) = 1.2; sex chromosome gain (4:1) = 1.7; homozygous frequency threshold = 0.95; homozygous value threshold = 0.8; heterozygous imbalance threshold = 0.4; minimum SNP probe density (Probes/MB) = 0.0; regions minimum size (Kbp) = 50. The HumanOmniExpress BeadChip SNP CMA experiment was carried out twice with similar results. The proband was evaluated at 12.5 years of age. He was born at term to a 15-year-old mother, with birth weight of 3266 g (25–50 percentile) and length of 53 cm (75–90 percentile). He had neonatal apnea that resolved without treatment and an otherwise unremarkable neonatal course. On initial hearing evaluation left conductive hearing loss was reported, but repeat testing was normal. The patient had numerous ear infections, frequent respiratory infections, and asthma. Gastroesophageal reflux had been diagnosed by pH probe. He underwent correction surgeries for right esotropia and cleft uvula and submucous cleft palate. Developmental testing revealed learning disabilities, fine and gross motor delays, and articulation difficulties. His WISC-III full-scale IQ was 90 when tested at 9 years and at 11 years of age. He had been diagnosed with panic disorder, anxiety disorder, attention deficit hyperactivity disorder, and adjustment disorder, and subsequently treated with Paroxetine and Methylphenidate. Additional clinical investigations had included magnetic resonance imaging of the brain, electroencephalogram, sleep study, and abdominal sonogram, all reported as normal. Echocardiography at age 11 was normal except for false tendons in the left ventricle. DNA testing for Fragile X and FISH for chromosome 22q11.2 microdeletion associated with velocardiofacial syndrome were normal. The biological mother was of Middle Eastern and Irish ancestry. She completed 10th grade, obtained a general education diploma, and was reported to have normal cognition. Both she and her maternal half-sister were reported to have pectus carinatum and leg length discrepancies. The boy's biological father was of Puerto Rican ancestry. Several paternal half-siblings were reported to have motor delays but no additional details are available. No relative was known to have DRS, cleft palate, or dysmorphic features. On examination at 12.5 years of age, height was 163.25 cm (95th percentile), weight was 47.25 kg (75th percentile), and head circumference was 53.9 cm (50th percentile). Bilateral DRS was noted and was more severe on the right (Fig. 2A and B). Dysmorphic features included synophrys, almond-shaped palpebral fissures, flat midface, high nasal bridge, malar hypoplasia, and inverted W-shaped posterior hairline (Fig. 2A). Ears were prominent and measured 6.9 cm (90th percentile). Palm length was 10.2 cm (85th percentile) and middle finger length was 8.2 cm (97th percentile). He had slight asymmetric pectus carinatum with hypoplastic right first rib noted on radiograph, mild metatarsus adductus, flat feet, and wide gap between the first and second toes. Pubic hair was Tanner II, with testes measuring 5 mL. Chromosome analysis revealed a pericentric inversion of chromosome 8 between the centromere and the long arm and mosaicism for a supernumerary marker chromosome: 47,XY,inv(8)(p11.1q13.2),+mar[11]/46,XY,inv(8)(p11.1q13.2)[9] (Fig. 3A). Multiplex fluorescence in situ hybridization (M-FISH) confirmed that the marker chromosome was derived from chromosome 8 (Fig. 3B). The parents were not available for participation and, thus, their karyotypes are not known. The proband's transformed lymphoblasts were analyzed by FISH to define the chromosome 8 inversion breakpoints. The 8p11.1 breakpoint was confirmed through successive BAC hybridizations; the centromere marker, CEP8, but no BAC clone was disrupted in mutant cells, consistent with the original karyotype (Fig. 4A–C). The 8q13.2 inversion breakpoint was defined by the inversion of probe RP11-50A22 at chr8:69,471,542-69,634,621 (Fig. 4A and B) but not of the more telomeric probes RP11-779P1 at chr8:69,621,417-69,803,905 (Fig. 4D, E) and RP11-865I6.2 at chr8:69,760,977-69,764,998 (Fig. 4F). Although inverted probe RP11-50A22 overlaps with noninverted probe RP11-779P1 by ~13 kb, we did not visualize a split of either BAC, suggesting the split occurs within or near the region of overlap. In a structurally normal chromosome 8, C8ORF34 maps to 8q13.2 and its 14 exons are transcribed in a centromeric (5′) to telomeric (3′) direction. The inverted BAC probe RP11-50A22 includes C8ORF34 exons 8–10, while the noninverted BAC probe RP11-779P1 includes C8ORF34 exons 10–14. Thus, these data support an intragenic breakpoint of C8ORF34 between exons 7–14, and map the telomeric breakpoint maximum critical region to 293 kb between hg19: chr8:69,471,542 and 69,764,998 defined by the start of RP11-50A22 and the end of RP11-865I6.2 (Fig. 4F). To define further the boundaries of the mosaic duplication arising from the marker chromosome, we undertook CMA of the proband's DNA. CMA analysis using the custom comparative genomic hybridization (CGH) oligonucleotide based microarray shows a 9 Mb copy gain spanning 8q11.2-q12.1 (hg19: chr8:51,488, 197-60,554,196); the duplicated region contains 38 genes but excludes CA8, RAB2A, CHD7, and CLVS1 (Fig. 5A). CMA using the SNP-based array revealed a larger and more complex duplication pattern which encompasses the region identified by the oligonucleotide array; the region spans 8p11.1-q12.3 and contains 56 genes (hg19: chr8:43,460,491-63,696,218) including CA8, RAB2A, CHD7, and CLVS1 (Fig. 5B-i–v). Notably, this region contains several contiguous but distinctive patterns of duplication. The pericentromeric area, highlighted in yellow (Fig. 5B-iii), has a Log R ratio of 0.39 within the smaller region on 8p11.1, but a ratio of only 0.15 within the larger region on 8q11.1-8q11.21. More remarkably, this region does not harbor the allelic imbalance predicted within a region of duplication, but instead reveals loss of heterozygosity (LOH). In contrast, the allele frequencies within the regions highlighted in purple (Fig. 5B-iv, v) harbor the anticipated allelic imbalance. In addition, the first purple region (Fig. 5B-iv) has a Log R ratio of 0.22 and corresponds closely to the region of duplication detected by the oligonucleotide array (Fig. 5A). Within the second region highlighted in purple (Fig. 5B-v) the Log R ratio falls to 0.15. Thus, the regions labeled 5B-ii and 5B-v both have Log R ratios very close to minimum threshold for copy number gain set at 0.13, and this might account for why the mosaicism was not detected by the oligonucleotide array. The 8q12-8q13 DURS1 locus is defined by two patients with syndromic DRS harboring deletions beginning at 8q12 and extending in the telomeric direction 4, 5 and one patient with a reciprocal translocation disrupting CPA6 on 8q13.2 6, 7. While all three patients had DRS, their accompanying syndromic features were quite variable. The definition of the DURS1 locus was then expanded by reports of three patients with DRS and 8q12 microduplications who shared syndromic features of DRS, dysmorphism, neonatal hypotonia, and motor developmental delay 9-11. Analyses of studies performed to date (Fig. 1) reveal that the deleted and the duplicated chromosomal regions within the DURS1 locus are nonoverlapping. Herein, we report a 12.5-year-old boy with syndromic DRS whose analysis further highlights the complexity of cytogenetic abnormalities that can occur at the DURS1-DRS locus. We find that he has a unique constellation of features associated with DRS, and has both a chromosome 8 inversion that transposes highly repetitive centromeric DNA and multiple 8q genes (8p11.1-8q13.2), and a complex mosaic supernumerary marker chromosome containing 8p11.1-8q12.3 material. Using FISH, we successfully mapped the telomeric inversion breakpoint to a 293 Kb interval within C8ORF34. C8ORF34 is a cDNA isolated from a human vestibular library which encodes an uncharacterized protein containing a putative cAMP-dependent protein kinase regulatory subunit expressed in adult brain, eye, ear, pituitary gland, thymus, kidney, and stomach (UCSC genome browser http://genome.ucsc.edu and Stanford SOURCE search gene report, http://source.stanford.edu). Thus, alteration or loss of C8ORF34 function in brain and eye could potentially contribute to the patient's DRS and intellectual and social disabilities. The translocation disrupting CPA6 7 and the inversion disrupting C8ORF34 (this report) support disruption of these genes, or regulatory elements, in DURS1-DRS. These genes are also deleted in the patient reported with branchiootorenal syndrome without DRS 8, however, suggesting that simply deleting these genes is not adequate to cause DRS, or that DRS is not fully penetrant. The chromosome 8 inversion also transposes highly repetitive centromeric DNA to the long arm of chromosome 8. We are not aware of phenotypic sequelae from germline changes in centromeric repetitive DNA sequence. There is, however, data suggesting that repetitive elements can have epigenetic influences on gene expression 14, 15. Thus, transposition of centromeric DNA may also contribute to the proband's phenotype. Concordant with our findings from the karyotype and FISH, interpretation of data from the two different CMA platforms is not straightforward, highlighting the complexity of the molecular mechanisms that have resulted in the apparent chromosomal rearrangement. While the custom CGH microarray demonstrates an 8q11.2-q12.1 duplication that does not include the four genes found to be in the 8q12 microduplication syndrome critical region, the SNP-based array reveals a larger region of duplication that includes these, as well as many additional genes. It has been reported that SNP-based arrays can detect subtle changes, such as low level mosaicism, that are missed on CGH 16, and thus we are confident that the extended duplicated region encompassing the 8q12 microduplication region in this patient is real. However, we cannot provide an explanation for the decrease in the level of mosaicism within the most telomeric portion of the duplicated region, nor for the LOH within the pericentromeric region. LOH regions identified from SNP-based arrays usually indicate consanguinity, uniparental disomy (UPD), or true copy number loss. We are unaware of a family history compatible with consanguinity, and no excess of LOH regions were observed in the CMA at the whole genome level. Notably, however, it has been suggested that at least one third of UPD cases emerge in connection with or due to a chromosomal rearrangement 17. As the LOH region identified in the q arm shows a borderline Log R ratio for a copy gain, it is possible that the region is actually in a euploid state. The proband shares some dysmorphic features and motor developmental delay with patients previously described with the germline 8q12 microduplication syndrome, suggesting that duplication of CA8, RAB2A, CHD7, and/or CLVS1 may contribute to DRS. He does not, however, share their heart and kidney malformations, he has less severe intellectual disabilities, and he has additional dysmorphisms, including a submucous cleft palate, not present in the previously described patients. These differences may reflect the low level of mosaic duplication in the patient or effects from the additional regions of duplication and inversion. Moreover, a fifth patient with an 8q12 microduplication encompassing these genes was reported to not have DRS 12, Thus, DRS may not be a fully penetrant feature of the 8q12 microduplication syndrome, or may arise from duplication of the 572 Kb region identified in the four patients with DRS but not in the patient without DRS (Fig 1). This region (hg19: chr8:60,219,746-60,792,079) is currently annotated by the UCSC Genome Browser to contain spliced ESTs and long noncoding RNAs but no protein-coding genes. In summary, these data suggest that the DURS1 locus could result in DRS by dosage effect in the region of 8q1, through deletion on 8q13 and/or a duplication of 8q12, or through alterations in gene expression arising from the inversion breakpoints or transposition of repetitive centromeric sequence. Thus, this case highlights the complexity of human disorders, and the potential requirement for multiple methods (including cytogenetics and different chromosomal microarray platforms) to gain insight into genotype–phenotype correlation, and ultimately into molecular mechanisms that underlie human disease. We thank the family for participating in the study. This study was supported by the National Institutes of Health (R01 EY15298). E. C. E. is a Howard Hughes Medical Institute Investigator. None declared.
Complex central nervous system (CNS) malformations frequently coexist with other developmental abnormalities, but whether the associated defects share a common genetic basis is often unclear. We describe five individuals who share phenotypically related CNS malformations and in some cases urinary tract defects, and also haploinsufficiency for the NFIA transcription factor gene due to chromosomal translocation or deletion. Two individuals have balanced translocations that disrupt NFIA. A third individual and two half-siblings in an unrelated family have interstitial microdeletions that include NFIA. All five individuals exhibit similar CNS malformations consisting of a thin, hypoplastic, or absent corpus callosum, and hydrocephalus or ventriculomegaly. The majority of these individuals also exhibit Chiari type I malformation, tethered spinal cord, and urinary tract defects that include vesicoureteral reflux. Other genes are also broken or deleted in all five individuals, and may contribute to the phenotype. However, the only common genetic defect is NFIA haploinsufficiency. In addition, previous analyses of Nfia−/− knockout mice indicate that Nfia deficiency also results in hydrocephalus and agenesis of the corpus callosum. Further investigation of the mouse Nfia+/− and Nfia−/− phenotypes now reveals that, at reduced penetrance, Nfia is also required in a dosage-sensitive manner for ureteral and renal development. Nfia is expressed in the developing ureter and metanephric mesenchyme, and Nfia+/− and Nfia−/− mice exhibit abnormalities of the ureteropelvic and ureterovesical junctions, as well as bifid and megaureter. Collectively, the mouse Nfia mutant phenotype and the common features among these five human cases indicate that NFIA haploinsufficiency contributes to a novel human CNS malformation syndrome that can also include ureteral and renal defects.
Abstract Abstract #1036 Background: Endothelial progenitor cells are critical to tumor angiogenesis and are increased in breast cancer patients. Copper is required for angiogenesis, and pre-clinical data suggest that tetrathiomolybdate (TM), a copper-depleting compound, inhibits angiogenesis and maintains tumor dormancy. We sought to measure circulating endothelial progenitor cells (CEPCs) in patients at high risk of breast cancer recurrence and to evaluate the effect of copper depletion on CEPCs.
 Methods: This analysis is part of an ongoing phase II study of TM in breast cancer patients at high risk of recurrence defined as Stage III or IV with no evidence of disease. All therapy other than hormonal was completed at least 6 weeks prior to study. Treatment: TM 180 mg daily to achieve a target ceruloplasmin (Cp) level of 5-15 mg/dL (copper depletion), and then 100 mg daily. We monitored levels of CEPCs (CD45dim, CD133+, VEGFR2+), CEA, CA15-3, and Cp at baseline and monthly. CEPCs were also measured in 6 healthy controls.
 Results: To date we have enrolled 16 patients with a median age of 51 years (range: 29-64). 14 had a history of Stage III disease, while 2 were considered to be Stage IV with no evidence of disease. The median number of positive lymph nodes among Stage III patients was 7 (1-42), with 2 patients having received neoadjuvant therapy. The median baseline Cp level was 28 mg/dL (21-41). Among 12 patients who have reached target Cp, the median time to target was 1 month (1-3 months). The median follow-up of the 4 patients who have not yet achieved target is 2.5 months. 1 of these discontinued treatment before reaching target. The median baseline CEPCs was lower in patients than healthy controls: 0.022 cells/μL (0.000-0.286) vs. 0.123 cells/μL (0.058-0.418); p=0.03. There was no statistically significant change in CEPCs from baseline over time.
 One patient was diagnosed with recurrent breast cancer at month 10. A rise in her CEPCs preceded a rise in a CEA and overt relapse by 1 and 5 months, respectively.
 Toxicity: Grade 3/4 neutropenia occurred in 3 patients. TM was held, and this resolved 5-13 days later, after which TM was resumed. No other grade 3/4 toxicity was observed. One patient discontinued TM due to diarrhea attributed to the lactose used in the compounding of TM.
 Conclusions: TM is well tolerated in breast cancer patients. We postulate that the increased CEPCs noted in one patient at month 4, 6 months prior to overt relapse, could represent the “turning on” of an angiogenic switch, resulting in an outpouring of CEPCs to the new site of metastasis. The trial is ongoing, and with additional follow-up other trends might emerge.
 Supported by Komen for the Cure Foundation, Anbinder Foundation, NY Community Trust and Breast Cancer Alliance of Greenwich. Citation Information: Cancer Res 2009;69(2 Suppl):Abstract nr 1036.
Abstract Background: Bone marrow-derived endothelial progenitor cells (EPCs) are critical for metastatic progression. Tetrathiomolybdate (TM), a copper-depleting compound inhibits angiogenesis and maintains tumor dormancy. This study explores the effect of TM on EPCs in patients (pts) at high risk for breast cancer (BC) recurrence. Methods: This phase II study enrolled Stage 3, 4 without evidence of disease (NED), and any node-positive triple negative (TN) BC pts. Only concomitant hormonal therapy was allowed. Pts received induction TM 180 mg daily at baseline (with exception of one pt) followed by an equal or lower daily dose (median 100mg, range 0-140) to maintain ceruloplasmin (Cp) level < 17 mg/dl (target for copper depletion). We monitored EPCs (CD45dim/CD133+/VEGFR2+), Cp, CEA and CA15-3 at baseline and monthly. To assess the association between Cp and EPCs over time, 3 independent mixed effects linear models with subject as a random effect were used to account for the correlation between observations on the same subject. All p-values were two-sided with statistical significance evaluated at the 0.10 alpha level. Results: 40 pts (28 adjuvant, 12 Stage 4 NED, 11 TN) were enrolled and 426 cycles of TM (average 10.65 per pt) were administered in the first 12 months on study. Median age was 50 yrs (29-66). Median number of tumor size and positive lymph nodes among adjuvant pts were 3.5 cm (1.2-7) and 9 (0-42), respectively. Of the patients receiving hormone therapy, 10 patients were on tamoxifen and 15 patients were on an aromatase inhibitor. 20% of patients were receiving a proton pump inhibitor (PPI). Median baseline Cp level was 28 mg/dL (20-47). 75% pts adequately copper depleted at month 1. 91% of TN patients copper depleted compared to hormone receptor positive subtypes (38-43%) and HER2/neu positive subtypes (40-67%). EPCs/ml decreased from baseline to last dose by 12 in pts that were copper-depleted (p=0.10) and by −52 in pts that did not achieve the copper depletion target (p=0.95). Multivariable modeling revealed an association of EPCs with Cp over time (p=0.005), with type of hormone therapy administered (p=0.007), and with co-administration of a PPI (p=0.0008). Six pts relapsed while on study in which a 200-fold increase in EPCs preceded an objective clinical relapse and a tumor marker rise by median of 1 month. Only grade 3/4 toxicity was hematologic, occurred in 20 cycles (4.7%) and resolved in 5-13 days with TM held and resumed at lower dose. Conclusions: TM is a well-tolerated oral copper chelator that may contribute to maintaining EPCs below baseline in copper-depleted pts. PPI may facilitate TM absorption. Molecular subtype and hormone therapy may impact on the ability to copper deplete. EPCs may have potential as a surrogate marker for early relapse and as a therapeutic target for interrupting the metastatic progression. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 2699. doi:1538-7445.AM2012-2699
<p>This section contains details of all mixed models utilized in the statistical analyses. The inferential tests performed in this paper necessitates a multiple comparisons adjustment, and details of the adjustment procedure is included in this section as well.</p>
