Elvira Muratore

Cairo University

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Luigi Bertolotti

University of Turin

Chiara Nogarol

Bitron (Italy)

Loretta Masoero

Istituto Zooprofilattico Sperimentale del Piemonte Liguria e Valle d'Aosta

Margherita Profiti

University of Turin

Sergio Rosati

University of Turin

Claudio Caruso

Azienda Sanitaria Locale CN2

Andreino Ponzo

Azienda Sanitaria Ospedaliera S.Croce e Carle Cuneo

Francesco Cerutti

Ospedale Amedeo di Savoia

Bryan Iotti

University of Turin

Barbara Colitti

University of Turin

Cooperative Institutions

University of Turin

Istituto Zooprofilattico Sperimentale del Piemonte Liguria e Valle d'Aosta

Cairo University

Torino e-district

Istituti di Ricovero e Cura a Carattere Scientifico

University of Padua

ASL Roma

University of Milan

National Research Council

National Veterinary Research Institute

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MODELE MATHEMATIQUE D'UNE FABRICATION DE PATE KRAFT BLANCHIE. I. PRINCIPES D'ELABORATION DU BILAN MATIERES EN REGIME PERMANENT

Elvira Muratore H Weisbuch P Monzie A. Ramaz

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Field application of an indirect gE ELISA on pooled milk samples for the control of IBR in free and marker vaccinated dairy herds

BMC Veterinary Research (2018)

Barbara Colitti Elvira Muratore Maria Elena Careddu Luigi Bertolotti Bryan Iotti

The aim of the present study was to assess the reliability of a new strategy for monitoring the serological response against Bovine Herpesvirus 1 (BoHV1), the causative agent of infectious bovine rhinotracheitis (IBR). Bulk milk samples have already been identified as cost effective diagnostic matrices for monitoring purposes. Nevertheless, most eradication programs are still based on individual standard assays. In a region of northwestern Italy (Piedmont), the voluntary eradication program for IBR has become economically unsustainable. Being the prevalence of infection still high, glycoprotein E-deleted marker vaccines are commonly used but gE blocking ELISAs are less sensitive on bulk milk samples compared to blood serum. A recently developed indirect gE ELISA showed high versatility when applied to a wide range of matrices. In this study, we applied a faster, cost effective system for the concentration of IgG from pooled milk samples. The IgG enriched fractions were tested using a gE indirect ELISA for monitoring purposes in IBR-positive and IBR-marker-vaccinated herds. Official diagnostic tests were used as gold standard. During a 3 years study, a total 250 herds were involved, including more than 34,500 lactating cows. The proposed method showed a very good agreement with official diagnostic protocols and very good diagnostic performances: only 37 positive animals were not detected across the entire study. The results highlighted the ability of the proposed method to support the surveillance of IBR in the Piedmont region, reducing the costs without affecting the diagnostic performances.

Bovine herpesvirus 1

Gold standard (test)

Infectious bovine rhinotracheitis

Bulk tank

10.1186/s12917-018-1716-5

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Citations (11)

[Neurological and psychiatric complications during cardiovascular pathology].

PubMed (1970)

Ettore Savagnone Elvira Muratore

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Una nuova metodica biomolecolare per la ricerca di Sarcocystis spp. nella carne bovina: dati preliminari

Francesco Chiesa Elvira Muratore Alessandra Dalmasso Tiziana Civera

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Development and validation of an indirect ELISA as a confirmatory test for surveillance of infectious bovine rhinotracheitis in vaccinated herds

BMC Veterinary Research (2015)

Luigi Bertolotti Elvira Muratore Chiara Nogarol Claudio Caruso Laura Lucchese

Bovine herpesvirus 1 (BoHV1) is a member of the viral subfamily of Alphaherpesvirinae that infects various species, including cattle, sheep, and goats. The virus causes infectious bovine rhinotracheitis (IBR), which is included in a European list of diseases that may require control and eradication programs. The lack of confirmatory tests affects the validity of diagnostic tools, especially those used for vaccinated herds. In this study, we report the development and validation of an indirect enzyme-linked immunosorbent assay (ELISA) based on BoHV1 glycoprotein E, which was expressed as a secreted recombinant antigen in a mammalian cell system. The performance of the new rec-gE ELISA was compared with that of commercially available indirect and/or blocking ELISAs. The sample set included blood sera from animals from IBR-positive farms, IBR-free farms, and marker-vaccinated farms. The indirect ELISA proposed in this study is based on antibody reactivity against BoHV1 gE, and showed high sensitivity and specificity (98.41 and 99.76 %, respectively). The ELISA performed well, in terms of both its diagnostic sensitivity and specificity, and as a confirmatory methodology, and therefore should improve the diagnostic protocols used for IBR surveillance.

Infectious bovine rhinotracheitis

Bovine herpesvirus 1

Alphaherpesvirinae

10.1186/s12917-015-0612-5

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Applicazione di un nuovo test ELISA indiretto per la ricerca di anticorpi totali verso il virus delle rinotracheite infettiva bovina su pools di sieri

Elvira Muratore Luigi Bertolotti Margherita Profiti Chiara Nogarol Claudio Caruso

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Feline Morbillivirus in Piedmont: A Pathological and Molecular Study

Journal of Comparative Pathology (2020)

Elvira Muratore Francesco Cerutti Elena Biasibetti Calogero Caruso C. Brovida

Morbillivirus

10.1016/j.jcpa.2019.10.098

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Evaluation of a new diagnostic tool for IBR surveillance in dairy herds: a field study in Cuneo province

Elvira Muratore Barbara Colitti Bryan Iotti Maria Elena Careddu Chiara Nogarol

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Feline morbillivirus in northwestern Italy: first detection of genotype 1-B

Journal of Feline Medicine and Surgery (2020)

Elvira Muratore Francesco Cerutti Elena Colombino Elena Biasibetti Claudio Caruso

Objectives A novel morbillivirus was recently described in stray and domestic cats in Asia, the USA and Europe. Most cats infected with feline morbillivirus (FeMV) showed lower urinary tract or kidney disease. Although the association of FeMV infection and kidney diseases has been suggested, the virus pathogenicity remains unclear. The present study aimed to investigate the distribution of FeMV infection, as well as the relationship between FeMV infection and kidney diseases in cats from northwestern Italy. Methods A total of 153 urine samples (150 individuals and three pools) and 50 kidney samples were collected and included in the study; total RNA was extracted and a reverse transcription quantitative PCR (RT-qPCR) was performed in order to identify FeMV. Kidneys were also submitted to anatomopathological examination. Phylogenetic analysis and isolation attempts were carried out on positive samples. In FeMV-positive cats, urinalysis and blood analysis were performed. Results FeMV RNA was detected in 7.3% of urine samples and in 8% of kidney samples, both in healthy cats and in cats with clinical signs/post-mortem lesions compatible with kidney disease. At histopathological examination, tubulointerstitial nephritis (TIN) was shown in 3/4 positive kidney samples, but a clear relationship between FeMV and TIN was not observed. Isolation attempts were unsuccessful, although the urine sample of one castrated male cat hosted in a cattery showed a positive signal in RT-qPCR until the fourth cell passage. Phylogenetic analysis revealed that this FeMV strain belonged to genotype 1-B. In the same cattery, a second genotype 1-B variant was detected from a urine pool. Urinalysis showed proteinuria in three cats, while at blood analysis three cats presented altered creatinine levels. Conclusions and relevance Data reported suggest the presence of a FeMV sub-cluster distinct from the strain previously isolated in Italy, whose role in renal disorders remains uncertain.

Morbillivirus

10.1177/1098612x20969360

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A new molecular approach to assess the occurrence of Sarcocystis spp. in cattle and products thereof: preliminary data

Italian Journal of Food Safety (2013)

Francesco Chiesa Elvira Muratore Alessandra Dalmasso Tiziana Civera

The genus Sarcocystis consists of more than 200 species. Those protozoa are characterised by a biological cycle composed by two obligatory hosts, definitive and intermediate. Apart from being possibly pathogenic for the intermediate host, a number of authors consider the intestinal sarcocystosis a minor zoonotic disease. Humans, in fact, can act as definitive host for two sarcosporidian species, S. suihominis e S. hominis, being infected through the consumption of raw or undercooked pig and bovine meat, respectively. Other two species could parasitise cattle: S. cruzi and S. hirsuta, having canids and felids as definitive hosts, respectively. The three species differentiate from each other in dimensions and cystic wall morphology, this latter being the basis for taxonomical studies. In 2010, the European Food Safety Authority (EFSA) highlighted the absence of reliable methods for epidemiological studies on the presence of Sarcocystis spp. in animals and products thereof. On this basis, the present study has been developed a new molecular method for the identification of Sarcocystis in bovine meat. For the development of the polymerase chain reaction (PCR) protocol, a set of samples of bovine meat from cattle (N=15), slaughtered at the didactic abattoir at the Veterinary Faculty of Turin University, has been collected, sequenced and used as reference samples during the study. A second set of samples (N=29), gathered from the same abattoir (N=12) and from abattoirs of Piedmont region (N=17), has been used for applicability tests. The overall positive rate for Sarcocystis spp. in our samples has been 91% (40/44), with S. cruzi representing the species with higher rates (68%), followed by S. hominis (43%) and S. hirsuta (2%). Based on the results of specificity and applicability tests performed in this study, the newly developed protocol proved to be reliable and suitable for epidemiologic purposes.

Sarcocystosis

Raw meat

10.4081/ijfs.2013.e41

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Citations (19)