The aim of the present study was to evaluate during 120 days the cultivation of the cyanobacteria Synechocystis sp. in urban secondary effluent in a pilot scale photobioreactor for the production of lipids, polyhydroxybutyrate (PHB) and phycobiliproteins. A 30 L semi-continuous photobioreactor fed with secondary effluent was used to produce biomass and evaluate the production of phycobiliproteins. Then, the harvested biomass was transferred into 5 L vertical column batch photobioreactors to perform PHB and lipid accumulation under nutrient starvation. Three hydraulic retention times (HRT) (6, 8 and 10 days) were tested in the growing phase photobioreactor to evaluate its influence on the biomass growth and microbial community. A maximum biomass concentration of 1.413 g L-1 and a maximum productivity of 173 mg L-1 d-1 was reached under an HRT of 8 days. Microscopy analysis revealed a shift from Synechocystis sp. to Leptolyngbya sp. and green algae when an HRT of 6 days was used. A continuous and stable production of phycobiliproteins was obtained, reaching a maximum of 7.4 %dcw content in the biomass. In the starvation phase, a PHB content of 4.8 %dcw was reach under 7 days of nitrogen and phosphorus starvation while 44.7 %dcw of lipids was achieved under nitrogen starvation.In conclusion, this work demonstrates the feasibility of cyanobacteria cultivation in wastewater to produce bio-based valuable compounds within a circular economy approach.
The boosting of greenhouse gas (GHG) emissions into the atmosphere due to anthropogenic activity contributes significantly to climate change. According to the Green Deal by 2050, net zero greenhouse gas emissions must be achieved. Therefore, actions are needed in order to control GHG emissions. The research presents and discusses the optimization of the microalgae biomass cultivation phase and the harvesting process in an advanced membrane photobioreactor (mPBR) with the aim to improve its production for green fuel generation. Experimental activities are carried out by considering Chlorella vulgaris microalgae as photosynthetic microorganism. A dark/light cycle of 12/12 hours was implemented by varying the light intensity from 100 to 300 μmol m-2 s-1. Different L/G rate, by keeping the gas flow rate (G) constant at 100 ml/min and increasing the liquid flow rate recirculation (L) from 500 to 1500 L min-1, has been tested to boost up the productivity of microalgae. Results highlight optimal production of microalgae biomass concentration up to 1.45 g L-1. Then a dynamic membrane module was implemented for the harvesting of the biomass. The work contributes to the field of climate change mitigation actions, by providing useful information to improve green energy production from algae biomass.
The resources on the planet are not unlimited and special attention has been paid to alternative energies in recent years. Alternative (or renewable) energy are all those energy sources that do not come from fossil fuels (coal, oil and natural gas). To date, wind and solar energy are the main sources of renewable energy on which huge investments has been made in recent years. However, many of these plants are no longer functional and need to be disused or upgraded. The aim of the following study is to show a technical and economic analysis carried out on some alternative energy plants, focusing on the social, economic and environmental aspects. In particular, the different possible scenarios were analyzed, in which the principles of reuse and recycling were applied, where the different plants from renewable sources were given a new life.
Using an environmentally friendly approach for eliminating methylene blue from an aqueous solution, the authors developed a unique electrospun nanofiber membrane made of a combination of polyethersulfone and hydroxypropyl cellulose (PES/HPC). SEM results confirmed the formation of a uniformly sized nanofiber membrane with an ultrathin diameter of 168.5 nm (for PES/HPC) and 261.5 nm (for pristine PES), which can be correlated by observing the absorption peaks in FTIR spectra and their amorphous/crystalline phases in the XRD pattern. Additionally, TGA analysis indicated that the addition of HPC plays a role in modulating their thermal stability. Moreover, the blended nanofiber membrane exhibited better mechanical strength and good hydrophilicity (measured by the contact angle). The highest adsorption capacity was achieved at a neutral pH under room temperature (259.74 mg/g), and the pseudo-second-order model was found to be accurate. In accordance with the Langmuir fitted model and MB adsorption data, it was revealed that the adsorption process occurred in a monolayer form on the membrane surface. The adsorption capacity of the MB was affected by the presence of various concentrations of NaCl (0.1-0.5 M). The satisfactory reusability of the PES/HPC nanofiber membrane was revealed for up to five cycles. According to the mechanism given for the adsorption process, the electrostatic attraction was shown to be the most dominant in increasing the adsorption capacity. Based on these findings, it can be concluded that this unique membrane may be used for wastewater treatment operations with high efficiency and performance.
Biodegradability is a growing interest in the field of research in Bioplastics. To what extent are we fully aware of the benign impact Bioplastic has on the environment, especially in water? Wastewater treatment plants are the primary source of microplastic release in the hydrographical environment. Almost 56% of annual plastic is discarded (19% recycled, 25% incinerated), the majority of which is discharged in the ocean. To reduce the negative impact of plastic waste on the environment, Bioplastics are chosen as an eco-friendly solution. Although Bioplastics are produced from renewable sources, it is a misbelief to consider all bioplastic harmless for the environment. As a matter of fact, most bioplastics are biodegradable but not compostable, a necessary criterion of sustainability. The behavior of micro-bioplastics in the aquatic environment is a complex ongoing area of research. The aim of this work is to give insight on the overlooked damaging nature of bioplastics in both production and disposal processes. Furthermore, a focus is placed on the recovery of bioplastics from wastewater by applying circular economy criteria and technology, including WWTPs processes.
Abstract The concept of nanomaterials membranes (NMs) promises to be a sustainable route to improve the membrane characteristics and enhance the performance of membrane bioreactors (MBRs) treating wastewater. This paper provided a critical review of recent studies on the use of membranes incorporating nanomaterials in membrane bioreactor (NMs-MBR) applications for wastewater treatment. Novel types of nanomaterials membranes were identified and discussed based on their structural morphologies. For each type, their design and fabrication, advances and potentialities were presented. The performance of NMs-MBR system has been summarized in terms of removal efficiencies of common pollutants and membrane fouling. The review also highlighted the sustainability and cost viability aspects of NMs-MBR technology that can enhance their widespread use in wastewater treatment applications.
Tannery wastewater contains large amounts of pollutants that, if directly discharged into ecosystems, can generate an environmental hazard. The present investigation has focused the attention to the remediation of wastewater originated from tanned leather in Tunisia. The analysis revealed wastewater with a high level of chemical oxygen demand (COD) of 7376 mgO2/L. The performance in reduction of COD, via electrocoagulation (EC) or UV photolysis or, finally, operating electrocoagulation and photolysis in sequence was examined. The effect of voltage and reaction time on COD reduction, as well as the phytotoxicity were determined. Treated effluents were analysed by UV spectroscopy, extracting the organic components with solvents differing in polarity. A sequential EC and UV treatment of the tannery wastewater has been proven effective in the reduction of COD. These treatments combined afforded 94.1 % of COD reduction, whereas the single EC and UV treatments afforded respectively 85.7 and 55.9 %. The final COD value of 428.7 mg/L was found largely below the limit of 1000 mg/L for admission of wastewater in public sewerage network. Germination tests of Hordeum Vulgare seeds indicated reduced toxicity for the remediated water. Energy consumptions of 33.33 kWh/m3 and 314.28 kWh/m3 were determined for the EC process and for the same followed by UV treatment. Both those technologies are yet available and ready for scale-up.