Noelia Mendoza

Universidad de Zaragoza

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J.A. Cebrián-Pérez

Universidad de Zaragoza

Rosaura Pérez‐Pé

Universidad de Zaragoza

T. Muiño‐Blanco

Universidad de Zaragoza

Raquel Ausejo

Universidad de Zaragoza

Adriana Casao

Universidad de Zaragoza

María Victoria Falceto

Universidad de Zaragoza

Joaquín Miguel

Universidad de Zaragoza

Adolfo Laviña

Universidad de Zaragoza

E. Fantova

Grupo ACS (Spain)

Olga Mitjana

Universidad de Zaragoza

Cooperative Institutions

Universidad de Zaragoza

Centro Regional de Selección y Reproducción Animal

Consejo Superior de Investigaciones Científicas

Clinica Universidad de Navarra

University of Córdoba

Centro de Investigación y Tecnología Agroalimentaria de Aragón

Instituto Aragonés de Ciencias de la Salud

Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria

Universidad de Murcia

Universidad de Navarra

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Bisphenol A Diglycidyl Ether (BADGE) and Progesterone Do Not Induce Ca2+ Signals in Boar Sperm Cells

Frontiers in Physiology (2020)

Anders Rehfeld Noelia Mendoza Raquel Ausejo Niels E. Skakkebæk

Aim: Exposure of boar sperm cells to Bisphenol A diglycidyl ether (BADGE) has been shown to lead to reproductive failure in sows, however, the mode of action is unknown. As we have recently shown that BADGE can interfere with Ca2+ signaling in human sperm cells through an action on CatSper, and as CatSper has been shown to be expressed in boar sperm cells, we hypothesized that a similar mechanism in the boar sperm cells could be responsible for the reproductive failure. Methods: Direct effects of BADGE and the endogenous ligand of human CatSper, progesterone, on Ca2+ signaling in human and boar sperm cells were evaluated side-by-side using a Ca2+ fluorimetric assay measuring changes in intracellular Ca2+. Effects of BADGE on Ca2+ signaling in boar sperm were furthermore assessed by flow cytometry by an independent laboratory. Results: The exact same solutions of BADGE and progesterone induced transient biphasic Ca2+ signals in human sperm cells, but failed to do so in both non-capacitated and capacitated boar sperm cells. BADGE also failed to induce transient biphasic Ca2+ signals in boar sperm cells in the flow cytometric assay. Conclusion: BADGE and progesterone failed to induce Ca2+ signals in boar sperm cells. This indicates that the signaling mechanisms leading to activation of CatSper differs between human and boar sperm cells, and suggests that the mode of action by which exposure of boar sperm cells to BADGE can lead to reproductive failure in sows does not involve effects on Ca2+ signaling.

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Capacitation

10.3389/fphys.2020.00785

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Significance of Non‐conventional Parameters in the Evaluation of Cooling‐induced Damage to Ram Spermatozoa Diluted in Three Different Media

Reproduction in Domestic Animals (2009)

Ignacio del Valle Noelia Mendoza Adriana Casao JÁ Cebrián‐Pérez Rosaura Pérez‐Pé

The objectives of this study were two. First, to compare three base media with different sugar composition as an initial step to achieve a good chemically-defined extender for ram sperm refrigeration. The second one, to determine which sperm quality parameters may be more useful for revealing differences between sperm samples. One medium contained 200 mM sucrose and 2.8 mM glucose (SM), another only disaccharides (D) such as sucrose, trehalose, maltose and lactose (75 mM each); and the third one (D+M) included a mix of monosaccharides (50 mM glucose, 20 mM fructose and 20 mM galactose,) and the same disaccharides as in D (50 mM each). Ram semen samples diluted in the mentioned media were refrigerated at 5°C for 1 h, and rewarmed upto 37°C in order to mimic the temperature in the female reproductive tract. Addition of monosaccharides to the extender did not produce a better preservation of motility or viability after cooling. The supplementation with other disaccharides apart from sucrose did not enhance the viability either. Thus, after cooling and rewarming, there were no significant differences in sperm viability (membrane integrity evaluated by CFDA/PI staining) or the percentage of progressive motile and rapid sperm (evaluated by CASA) between the three media. However, the percentage of viable non-capacitated sperm evaluated by the chlortetracycline (CTC) assay was higher and sperm oxygen consumption was lower in SM than in D and in D+M. Although the apoptosis-like markers [phosphatidylserine exposure assessed by Annexin V/CFDA staining and DNA-damage evaluated by TUNEL assay] showed a continuous increment throughout the process with all diluents, the percentage of sperm with damaged DNA at the end of the process was significantly lower in SM than in the other two media (p < 0.01). On the basis of these results, we would make two recommendations: the use of an extender supplemented only with sucrose and glucose for ram sperm refrigeration; the inclusion of non-conventional methods such as oxygen consumption measure, evaluation of capacitation state and apoptosis-like markers for revealing differences between sperm samples.

Monosaccharide

10.1111/j.1439-0531.2009.01552.x

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Cryopreservation of aoudad (Ammotragus lervia sahariensis) sperm obtained by transrectal ultrasound-guided massage of the accessory sex glands and electroejaculation

Theriogenology (2012)

J. Santiago‐Moreno C. Castaño Adolfo Toledano‐Díaz M.C. Esteso A. López-Sebastián

Electroejaculation

10.1016/j.theriogenology.2012.10.011

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Citations (53)

Chromatin status in human ejaculated spermatozoa from infertile patients and relationship to seminal parameters

Human Reproduction (2001)

Juan Antonio Maldonado Molina José Antonio Castilla Julio Castaño Juan Torres Fontes Noelia Mendoza

The aim of this study was to evaluate the chromatin status in different groups of patients. Five groups of men were selected: pre-vasectomy; male factor infertility; varicocele; immunological male infertility; and idiopathic infertility. Chromatin status was evaluated using flow cytometry after staining the DNA with the fluorochrome propidium iodide. Differences were observed in the state of sperm chromatin between the male factor and varicocele groups with respect to the others. These two groups presented poorer quality chromatin, as evidenced fundamentally by a lower degree of condensation. These deficiencies in chromatin status were usually accompanied by alterations in the other standard parameters of semen analysis. Individuals who are infertile due to male factor and those presenting varicocele have spermatozoa with less condensed chromatin which might, in part, explain their sterility.

Semen Analysis

10.1093/humrep/16.3.534

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Citations (22)

Relationship between apoptotic markers and fertility in ram semen.

XIV Jordanas Sobre Produccion Animal, Zaragoza, Espana, 17 y 18 de Mayo de 2011. (2011)

Noelia Mendoza Adriana Casao Rosaura Pérez‐Pé F. Quintín E. Sevilla

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Photoperiod and Melatonin Supplementation: Variable Effects on the Quality of Chilled Dog Semen

Frontiers in Veterinary Science (2022)

Olga Mitjana Raquel Ausejo Noelia Mendoza Joaquín Miguel María Teresa Tejedor

The addition of melatonin in seminal extenders due to its antioxidant properties and its beneficial role in sperm preservation has been previously described, especially in seasonal species. The aim of this study was to study a potential seasonal effect based on photoperiod duration when adding a physiological concentration of melatonin in the canine ejaculate. A total of 24 ejaculates were obtained from 10 healthy dogs during the increasing photoperiod (from December 21 to June 21), whereas 12 ejaculates were collected from five healthy individuals during the decreasing photoperiod (from June 22 to December 20). Each ejaculate was separated into two aliquots, and one of them remained as a control, whereas melatonin (100 pM) was added to the other one (C and M treatment groups, respectively). Diluted semen was refrigerated at 5°C. On days 0, 1, 2, 3, and 6, sperm motility analyses were performed using a CASA system and hypoosmotic swelling test (HOST), osmotic resistance test (ORT), and flow cytometry analysis. No effect of melatonin on motility was detected in either photoperiod. Negative effects of melatonin were found for acrosomal defects, apoptosis, and viability in the decreasing photoperiod. The addition of melatonin to sperm in the decreasing photoperiod could create such a high level that it would cause the described negative effects. We found a beneficial effect of melatonin in the increasing photoperiod on acrosomal defects and apoptosis during 0-6 days. Melatonin treatment also increased viability in the short term (days 1 and 2) for both photoperiods. Also, melatonin can provide certain beneficial effects on mitochondrial activity in the medium term (days 2 and 3) in the decreasing photoperiod.

10.3389/fvets.2022.956630

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A new protocol for the assessment of active caspases in ram spermatozoa.

XXXIX Jornadas de Estudio, XIII Jornadas sobre Producción Animal, Zaragoza, España, 12 y 13 de mayo de 2009 (2009)

Noelia Mendoza Rosaura Pérez‐Pé J.A. Cebrián-Pérez T. Muiño‐Blanco

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Contaminación bacteriana por biofilm en un centro de inseminación artificial de porcino

Anaporc: revista de la Asociación de Porcinocultura Científica (2016)

Raquel Ausejo Noelia Mendoza J. C. Miguel Yahya Dahmani Erika Fuentes

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Expression of annexins 1 and 5 in human pituitary tumours

The Journal of Physiology (2000)

Abeda Mulla Helen Christian John F. Morris Noelia Mendoza Egle Solito

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Nuclear DNA Fragmentation in Boar Spermatozoa: Measurement Methods and Reproductive Performance Implications

Frontiers in Veterinary Science (2022)

Raquel Ausejo Juan Manuel Martínez Noelia Mendoza Alfonso Bolarín María Teresa Tejedor

The aim of this research was to compare the different techniques to measure sperm nuclear DNA fragmentation (sDF) and to check its relations to boar reproductive value, classical spermiogram parameters, and reproductive results of the doses in sows. Sperm chromatin stability assay (SCSA), terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, and sperm chromatin dispersion test (SCD, Halomax ® ) results were compared, finding a statistically significant correlation only between SCSA and TUNEL results. The fertility direct boar effect (DBE) index, calculated from the whole productive life of the boar, was not correlated ( p > 0.05) with sDF (measured by any technique). Total or progressive sperm motility was not correlated with sDF, while it found a positive correlation between TUNEL measure and abnormal acrosomes (%) and between SCD measure and total sperm morphological abnormalities (%). No significant correlations were obtained between fertility or prolificacy results and sDF results with the different techniques. However, in the case of total born and SCSA measure, the correlation was close to significance (r partial = −0.095; p = 0.066), appointing to a tendency; as SCSA increases, the number of total piglets born decreases. In conclusion, although the different techniques for the sDF seem not to target exactly the same DNA events and the relationship between their values and the reproductive results and the classical spermiogram results is still to be elucidated, the studied sDF techniques may offer extra information that could be useful for the management of AI studs.

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10.3389/fvets.2022.929858

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Citations (3)