Yuriy V. Shebzukhov

Research Field

Supplementary Figure 1 from Alternative Variants of Human HYDIN Are Novel Cancer-Associated Antigens Recognized by Adaptive Immunity

Karoline Laske Yuriy V. Shebzukhov Ludger Große‐Hovest Dmitry V. Kuprash Svetlana V. Khlgatian

PDF - 395KB, Supplementary Figure 1: Structure of MO-TES391 and KIAA1864 transcripts.

10.1158/2326-6066.22537066

Cite

Citations (0)

Supplementary Figure 1 from Alternative Variants of Human HYDIN Are Novel Cancer-Associated Antigens Recognized by Adaptive Immunity

Karoline Laske Yuriy V. Shebzukhov Ludger Große‐Hovest Dmitry V. Kuprash Svetlana V. Khlgatian

PDF - 395KB, Supplementary Figure 1: Structure of MO-TES391 and KIAA1864 transcripts.

10.1158/2326-6066.22537066.v1

Cite

Citations (0)

Accelerated thymic atrophy as a result of elevated homeostatic expression of the genes encoded by the TNF/lymphotoxin cytokine locus

European Journal of Immunology (2009)

Dmitry J. Liepinsh Andrey Kruglov A R Galimov Alexander N. Shakhov Yuriy V. Shebzukhov

TNF, lymphotoxin (LT)-alpha, LT-beta and LIGHT are members of a larger superfamily of TNF-related cytokines that can cross-utilize several receptors. Although LIGHT has been implicated in thymic development and function, the role of TNF and LT remains incompletely defined. To address this, we created a model of modest homeostatic overexpression of TNF/LT cytokines using the genomic human TNF/LT locus as a low copy number Tg. Strikingly, expression of Tg TNF/LT gene products led to profound early thymic atrophy characterized by decreased numbers of thymocytes and cortical thymic epithelial cells, partial block of thymocyte proliferation at double negative (DN) 1 stage, increased apoptosis of DN2 thymocytes and severe decline of T-cell numbers in the periphery. Results of backcrossing to TNFR1-, LTbetaR- or TNF/LT-deficient backgrounds and of reciprocal bone marrow transfers implicated both LT-alpha/LT-beta to LTbetaR and TNF/LT-alpha to TNFR1 signaling in accelerated thymus degeneration. We hypothesize that chronic infections can promote thymic atrophy by upregulating LT and TNF production.

Lymphotoxin alpha

Thymocyte

Cytokine receptor

Thymic involution

Lymphotoxin beta receptor

Homeostasis

10.1002/eji.200839191

Cite

Citations (33)

Ipr1 gene mediates innate immunity to tuberculosis

Nature (2005)

Hui Pan Bo‐Shiun Yan Mauricio Rojas Yuriy V. Shebzukhov Hongwei Zhou

Intracellular parasite

Congenic

10.1038/nature03419

Cite

Citations (472)

Cellular source and molecular form of TNF specify its distinct functions in organization of secondary lymphoid organs

Blood (2010)

Alexei V. Tumanov Sergei I. Grivennikov Andrey Kruglov Yuriy V. Shebzukhov Ekaterina P. Koroleva

Follicular dendritic cells

10.1182/blood-2009-10-249177

Cite

Citations (92)

Supplementary Figure Legends from Alternative Variants of Human HYDIN Are Novel Cancer-Associated Antigens Recognized by Adaptive Immunity

Karoline Laske Yuriy V. Shebzukhov Ludger Große‐Hovest Dmitry V. Kuprash Svetlana V. Khlgatian

PDF - 88KB, Supplementary Figure Legends.

10.1158/2326-6066.22537060

Cite

Citations (0)

Supplementary Table 3 from Alternative Variants of Human HYDIN Are Novel Cancer-Associated Antigens Recognized by Adaptive Immunity

Karoline Laske Yuriy V. Shebzukhov Ludger Große‐Hovest Dmitry V. Kuprash Svetlana V. Khlgatian

PDF - 49KB, Supplementary Table 3: Epitope prediction for HLA-A*02 binding peptides derived from KIAA1864 and MO-TES391.

Table (database)

10.1158/2326-6066.22537051.v1

Cite

Citations (0)

Alternative Variants of Human HYDIN Are Novel Cancer-Associated Antigens Recognized by Adaptive Immunity

Cancer Immunology Research (2013)

Karoline Laske Yuriy V. Shebzukhov Ludger Große‐Hovest Dmitry V. Kuprash Svetlana V. Khlgatian

A mutation in the hydin gene has been recently described as one possible mechanism leading to lethal congenital hydrocephalus in mice, and a similar defect is proposed to be involved in an autosomal recessive form of hydrocephalus in human. Here, we report for the first time on the cancer association and immunogenicity of two HYDIN variants in humans. One is a previously described sequence derived from the chromosome 1 gene copy, that is, KIAA1864. The second is encoded by a novel alternative transcript originating from the chromosome 16, which we identified by immunoscreening of a testis-derived cDNA expression library with sera of patients with colorectal cancer, and called MO-TES391. Both variants are targeted by immunoglobulin G antibodies in a significant subset of cancer patients but only rarely in healthy donors. Moreover, we identify HLA-A*0201-restricted sequences derived from MO-TES391 and KIAA1864, which are specifically recognized by human cytotoxic CD8(+) T cells. Taken together, these results suggest frequent and coordinated adaptive immune responses against HYDIN variants in patients with cancer and propose HYDIN as a novel cancer-associated antigen.

10.1158/2326-6066.cir-13-0079

Cite

Citations (17)

S2.2 ‐ Antigen Processing and Activation

European Journal of Immunology (2009)

Dmitry V. Kuprash Andrey Kruglov Yuriy V. Shebzukhov Sergei I. Grivennikov Dmitry J. Liepinsh

Abstract No Abtract

10.1002/eji.200990080

Cite

Citations (0)

Supplementary Table 1 from Alternative Variants of Human HYDIN Are Novel Cancer-Associated Antigens Recognized by Adaptive Immunity

Karoline Laske Yuriy V. Shebzukhov Ludger Große‐Hovest Dmitry V. Kuprash Svetlana V. Khlgatian