Robert P. Stephan

University of Arizona

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Co-Authors

Max D. Cooper

Emory University

Pamela L. Witte

Loyola University Chicago

Randall S. Davis

University of Alabama at Birmingham

Deborah A. Lill-Elghanian

Loyola University Medical Center

Woong-Jai Won

University of Alabama at Birmingham

David K. Crossman

University of Alabama at Birmingham

R. Glenn King

University of Alabama at Birmingham

Kazuhito Honjo

University of Alabama at Birmingham

André M. Vale

Universidade Federal do Rio de Janeiro

Lara Ianov

University of Alabama at Birmingham

Cooperative Institutions

University of Alabama at Birmingham

University of California, Los Angeles

Howard Hughes Medical Institute

Emory University

Versiti Blood Center of Wisconsin

Stanford University

Medical College of Wisconsin

Scripps Research Institute

UCLA Jonsson Comprehensive Cancer Center

Charité - Universitätsmedizin Berlin

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FCRL6 distinguishes a subset of biologically impaired mouse pro B cells (153.16)

The Journal of Immunology (2011)

Woong-Jai Won Robert P. Stephan Randall S. Davis

Abstract Human Fc receptor-like 6 (FCRL6) is discretely expressed by cytotoxic lymphocytes, has ITIM-based signaling potential, and interacts with MHC II. To investigate the biology of its counterpart in mice, a panel of FCRL6-specific monoclonal antibodies was generated. In contrast to its expression in humans, FCRL6 could not be detected on mouse NK or CD8+ T cells. Instead, the receptor marked a subpopulation of pro B cells in adult bone marrow. FCRL6+ cells were absent in Hardy Fr. A, but became evident in Fr. B, peaked in Fr. C, and diminished in Fr. C’. The developmentally regulated expression of FCRL6 was also indicated by the increased frequency of these pro B cells in RAG2-/- and λ5-/- mice. FCRL6+ pro B cells were slightly smaller by light scatter and expressed relatively lower levels of IL-7Rα compared to FCRL6- cells. In keeping with this phenotypic difference, FCRL6+ pro B cells responded less favorably to IL-7 stimulation. They also had relatively lower proliferative and cycling capacity. Exposure of purified FCRL6- pro B cells to cytokines important for early B lineage differentiation revealed that FCRL6 surface expression could be induced by IL-7 alone, but was augmented by the addition of SCF. Collectively, these results demonstrate that FCRL6 defines a unique subset of mouse pro B cells that competes poorly relative to cells lacking its expression and suggest that FCRL6 may distinguish a subset of biologically impaired pro B cells.

10.4049/jimmunol.186.supp.153.16

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Student Feedback Regarding Online Course Production Value: A Case Study from Classics

Robert P. Stephan

This paper employs a data-driven approach to understanding the relationship between online course production quality, instructional effectiveness, and student satisfaction. To accomplish this, it assesses data from six years of student evaluations for online courses and compares it to the production style and quality present in those courses. The quantitative multiple choice course evaluation questions speak to issues of overall quality and satisfaction, student learning, and content and lesson clarity. Qualitative student comments are also assessed to understand the degree to which production quality is highlighted as a driving factor in both student enjoyment and instructional effectiveness. Finally, trends in evaluation data are analyzed in conjunction with the quality and style of each course to provide a better understanding of how students perceive the value of production quality. This project serves as an important step in furthering evidence-based online pedagogy and provides valuable insights for how to best allocate time and resources with regard to online course production.

CLARITY

Online course

Value (mathematics)

10.23919/pnc56605.2022.9982733

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Characterization of Paired Ig-Like Receptors in Rats

The Journal of Immunology (1999)

Glynn Dennis Robert P. Stephan Hiromi Kubagawa Max D. Cooper

To explore the phylogenetic history of the murine paired Ig-like receptors of activating (PIR-A) and inhibitory (PIR-B) types, we isolated PIR homologues from a rat splenocyte cDNA library. The rat (ra) PIR-A and raPIR-B cDNA sequences predict transmembrane proteins with six highly conserved extracellular Ig-like domains and distinctive membrane proximal, transmembrane, and cytoplasmic regions. The raPIR-B cytoplasmic region contains prototypic inhibitory motifs, whereas raPIR-A features a charged transmembrane region and a short cytoplasmic tail. Southern blot analysis predicts the presence of multiple Pira genes and a single Pirb gene in the rat genome. Although raPIR-A and raPIR-B are coordinately expressed by myeloid cells, analysis of mRNA detected unpaired expression of raPIR-A by B cells and raPIR-B by NK cells. Collectively, these findings indicate that the structural hallmarks of the Pir gene family are conserved in rats and mice, yet suggest divergence of PIR regulatory elements during rodent speciation.

Northern blot

Southern blot

10.4049/jimmunol.163.12.6371

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Citations (23)

Student Feedback on Archaeogaming: Perspectives from a Classics Classroom

Zenodo (CERN European Organization for Nuclear Research) (2023)

Robert P. Stephan

10.5281/zenodo.8221602

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Development of B cells in aged mice: decline in the ability of pro-B cells to respond to IL-7 but not to other growth factors.

The Journal of Immunology (1997)

Robert P. Stephan Deborah A. Lill-Elghanian Pamela L. Witte

The process of B lymphopoiesis changes with increasing age, resulting in a severe drop in the number of pre-B cells in old mice. We have shown that the ability of freshly isolated pro-B cells to proliferate on stromal cells declines with age. In this study, we wanted to determine the reason for the diminished response of the aged pro-B cells. The functional alterations could arise from changes intrinsic to the pro-B cells or to composition of the precursor population. Changes in the composition of pro-B cells in marrow or long-term bone marrow culture system for B lymphocytes (LTBMC-B) could not account for the functional losses. We then examined the hypothesis that the diminished amount of proliferation on stromal cells was due to changes in the responsiveness to stroma-derived cytokines. The proliferation to IL-7 but not to stem cell factor (SCF) or insulin-like growth factor 1 (IGF-1) was severely impaired by 24 mo of age, independently of the concentration of IL-7 and length of culture time. The reduced IL-7 response could not be explained by an increase in the percentage of cells undergoing apoptosis; rather, a greater frequency of aged cells remained in G0/G1 after IL-7 stimulation. Furthermore, the impaired responsiveness of the aged pro-B cells is not due to diminished expression of the IL-7R alpha-chain or the common gamma-chain. Since only IL-7 responsiveness is impaired, we believe that the underlying molecular mechanism for the cellular alterations is specific to signaling through the IL-7 receptor complex.

Lymphopoiesis

10.4049/jimmunol.158.4.1598

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Citations (133)

The Bays of East Attica Regional Survey 2020–2021: New Evidence for Settlement, Production, and Exchange in Porto Rafti, Greece, from Prehistory to Late Antiquity

Mouseion Journal of the Classical Association of Canada (2023)

Sarah C. Murray Melanie Godsey Joseph Frankl Bartłomiej Lis Grace Erny

This paper presents the work of the Bays of East Attica Regional Survey project in 2020 and 2021. The project focuses on documenting evidence of human occupation and land use around the bay of Porto Rafti in eastern Attica, Greece. Work in 2020 and 2021 built on the progress achieved in the 2019 pilot season, which established the presence of a Final Neolithic/Early Bronze Age obsidian processing centre on the Pounta peninsula, a sizeable Late Helladic IIIC settlement on the islet of Raftis, and some minor pre- and post-Ptolemaic activity on the Koroni peninsula, previously assessed as a single-period military camp. Fieldwork in 2021 documented a dense, multi-period surface scatter on the islet of Praso, including Final Neolithic/Early Bronze Age, Late Bronze Age, Archaic, Classical, Hellenistic, Late Roman, Byzantine, and Ottoman material. Remains of waste products from the manufacture of Late Helladic IIIC White Ware, Classical/Hellenistic tile, Late Roman tile, and metal indicate the frequentative presence of industrial activity. Additional study of material from the 2019 season in 2020 and 2021 provided new insights into the chronological and typological character of material from Pounta, Koroni, and Raftis. The survey data from the first two field seasons of the project contribute to an increasingly rich and detailed reconstruction of society and economy in Porto Rafti bay throughout antiquity.

Peninsula

Bronze

Settlement (finance)

10.3138/mous.19.2.01

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Citations (2)

EMAP Pague Well - LA130191 Unit 43, 2002

Maren P. Hopkins Stephanie Kulow Siri L. Angeles Robert P. Stephan

Source

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Role of Stromal Cells in Protecting Young and Aged B-Lineage Precursors from Dexamethasone-Induced Apoptosis

The Scientific World JOURNAL (2001)

Jeannette Pifer Robert P. Stephan Deborah A. Lill-Elghanian Pamela L. Witte

This article has no abstract.

Lineage (genetic)

10.1100/tsw.2001.148

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The 2019 Bays of East Attica Regional Survey (BEARS) Project: New Archaeological Evidence for the History of Coastal Settlement, Economy, and Connectivity from Porto Rafti (Greece)

Mouseion Journal of the Classical Association of Canada (2021)

Sarah C. Murray Catherine E. Pratt Robert P. Stephan Maeve McHugh Grace Erny

This paper presents the findings of the inaugural season of the Bays of East Attica Regional Survey project (BEARS). The project aims to clarify the history of human activity around the bay of Porto Rafti in eastern Attica. Surface finds from Raftis Island demonstrate that it was the location of a major Late Helladic IIIC site probably linked to the cemetery at Perati, as well as of limited Late Roman occupation. The Pounta peninsula yielded a large quantity of obsidian lithics, indicating significant activity during the Final Neolithic/Early Bronze Age, with lesser quantities of material dated to the Late Helladic IIIC and Roman/Byzantine periods. At Koroni, surface finds of Late Helladic IIIC and Archaic/Classical date indicate that activity at the site predates the third-century Ptolemaic military camp excavated by American archaeologists in 1960. Overall, these survey data provide a range of new evidence and insight into the history of the Porto Rafti area and its connections to other regions of the Aegean. Methodologically, the project’s work also demonstrates the value of conducting archaeological surface survey even in areas with extensive modern development.

Peninsula

Settlement (finance)

Archaeological Evidence

10.3138/mous.17.2.005

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Citations (7)

Student Feedback On Archaeogaming: Perspectives from a Classics Classroom

Journal of Computer Applications in Archaeology (2024)

Robert P. Stephan

This study assesses student feedback from the implementation of Assassin's Creed: Odyssey as a teaching tool in a lower level, general education Classics course (CLAS 160B1: Meet the Ancients: Gateway to Greece and Rome).In this course, which ran in Fall 2021, students were given the option to choose either a video game-based assignment sequence or a traditional reading response assignment sequence.Both assignment sequences required similar activities and output: reading primary and secondary source literature, reflecting on a question prompt, and writing an essay in response to the prompt.The primary difference was that students who opted for the video game sequence also completed a series of tasks within the video Assassin's Creed: Odyssey.After the course, students were provided with a short quantitative and qualitative survey in which they could reflect upon their learning experience, and these results were then compared between the students who opted for the video game-based assignment sequence and the more traditional assignment sequence.Preliminary results show that students who opted for the video game assignment sequence gave, on average, higher ratings with regard to their enjoyment of the course assignments and with regard to the amount of learning they felt they achieved.This suggests that the implementation of video game-based pedagogy may be a useful strategy for history, archaeology, and classics courses in the future.Caution is urged, however, due to the small sample size, the non-random sampling strategy, and the lack of objective learning outcome measurement.

10.5334/jcaa.139

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Citations (1)