Tingsong Li

Children's Hospital of Chongqing Medical University

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Siqi Hong

China International Science and Technology Cooperation

Shubin Yan

Zhejiang University of Water Resource and Electric Power

Lifang Shen

Zhejiang University of Water Resource and Electric Power

Pengwei Liu

Agriculture and Forestry University

Yifeng Ren

North University of China

Jiannan Ma

Children's Hospital of Chongqing Medical University

Shuang Liao

Children's Hospital of Chongqing Medical University

Yuanyuan Luo

Shanghai East Hospital

Xiaoyu Zhang

First Affiliated Hospital of Anhui Medical University

Jilai Liu

Zhejiang University of Water Resource and Electric Power

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Chongqing Medical University

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Children's Hospital of Chongqing Medical University

China International Science and Technology Cooperation

Chinese Academy of Sciences

Sun Yat-sen University

Zhejiang University

National Clinical Research

Zhejiang University of Water Resource and Electric Power

Huazhong University of Science and Technology

Ministry of Education of the People's Republic of China

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STUDY ON THE DAMAGE OF CULTURED HIPPOCAMPAL NEURONS INDUCED BY SEIZURE-LIKE DISCHARGE AND THE EFFECT OF BRAIN-DERIVED NEUROTROPHIC FACTOR ON THE INJURED NEURONS

PEDIATRICS (2008)

Li Jiang Ming Zhang Tingsong Li

OBJECTIVE: The objective of this study was to observe the damage of cultured hippocampal neurons induced by seizure-like discharge and study the effects of brain-derived neurotrophic factor (BDNF) on such injury. METHODS: Primary cultured hippocampal neurons were randomly divided into 3 groups: (1) control group: cultured neurons were exposed to regular extracellular solution for 3 hours, then returned to regular medium; (2) seizure-like discharge group: cultured neurons were exposed to magnesium-free extracellular solution for 3 hours, then maintained for 24 hours in regular medium; and (3) BDNF-treated group: cultured neurons were precultured with regular medium added to BDNF (200 ng/mL) for 24 hours and exposed to magnesium-free extracellular solution for 3 hours, then maintained for 24 hours in regular medium that contained BDNF. The morphologic changes of neurons dyed by acridine orange/ethidium bromide were observed. Mitochondria membrane potential (MEP) by JC-1 dye was assessed with laser scanning confocal microscope. Lactic acid dehydrogenase (LDH) in supernatant was detected by autobiochemical analyzer. BDNF was detected by immunocytochemistry and assessed by optical density. RESULTS: There were some apoptotic and necrotic neurons in the seizure-like discharge group. Compared with the control group, MEP was significantly decreased and LDH level and BDNF expression were significantly increased in the seizure-like discharge group. Compared with the seizure-like discharge group, MEP was significantly increased and LDH level was significantly decreased in BDNF-treated group, but there was no significant difference on BDNF expression between them. CONCLUSIONS: Seizure-like discharge could induce injury to hippocampal neurons and could upregulate BDNF expression in hippocampal neurons. BDNF could relieve the damage of neurons induced by seizure-like discharge, so BDNF has protective effects on hippocampal neurons.

Acridine orange

NeuN

10.1542/peds.2007-2022llllll

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Clinical characterization of epilepsy in children with chromosomal aberration 47, XXY

Brain and Behavior (2023)

Congjie Chen Yuanyuan Luo Xueqing Hou Tingsong Li

Abstract Objective The phenotype of the chromosomal aberration 47, XXY exhibits considerable heterogenicity. In addition, epilepsy is extremely uncommon in individuals with this chromosomal disorder. As a result, the clinical characteristics of epilepsy in these patients remain poorly understood. Methods Clinical data and the evolution of epilepsy in a boy diagnosed with chromosomal aberration 47, XXY were collected and analyzed. Furthermore, a systematic literature review was conducted to examine the relationship between chromosomal aberration 47, XXY and epilepsy in children. Results We identified a novel phenotype associated with the chromosomal anomaly 47, XXY in a 2‐year‐2‐month‐old boy who presented with self‐limited epilepsy with autonomic seizures at onset, followed by developmental and/or epileptic encephalopathy with spike‐wave activation in sleep (D/EE‐SWAS), which was responsive to corticosteroid treatment. Including the present case, we analyzed 21 cases of children diagnosed with epilepsy due to the presence of the 47, XXY chromosomal anomaly. The most common types of epilepsy were focal combined generalized epilepsy ( n = 9), epileptic spasms ( n = 6), and generalized epilepsy ( n = 4). There were six cases of infantile epileptic spasm syndrome (IESS) ( n = 5) and developmental and epileptic encephalopathy ( n = 1), one case of Lennox–Gastaut syndrome, and one case of D/EE‐SWAS. Apart from corticosteroids in IESS, 15 antiseizure medications (ASMs) were prescribed to eight children in this cohort, with valproate ( n = 5) being the most frequently used. Conclusions The epilepsy types and syndromes associated with the chromosomal anomaly 47, XXY demonstrated considerable heterogeneity. Among the observed phenotypes, IESS and focal epilepsy, which displayed partial responsiveness to multiple ASMs, were the most prevalent.

Epilepsy syndromes

West Syndrome

Epileptic spasms

10.1002/brb3.3178

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Characteristics and outcomes of children with dissociative (conversion) disorders in western China: a retrospective study

BMC Psychiatry (2021)

Zhixu Fang Yuhang Li Lingling Xie Min Cheng Jiannan Ma

Abstract Background Dissociative (conversion) disorder in children is a complex biopsychosocial disorder with high rates of medical and psychiatric comorbidities. We sought to identify the characteristics and outcomes of children with dissociative (conversion) disorders in western China. Methods We conducted a retrospective cohort study of 66 children admitted with dissociative (conversion) disorders from January 2017 to July 2019, and analyzed their clinical characteristics, socio-cultural environmental variables, and personality and psychiatric/psychological characteristics. Binary logistic regression was used to analyze the variables associated with clinical efficacy. Results Of these 66 patients, 38 (57.6%) were male and 28 (42.4%) were female, 46 (69.7%) had an antecedent stressor, 30 (45.5%) were left-behind adolescents, and 16 (24.2%) were from single-parent families. In addition, 30 patients (45.5%) were not close to their parents, 38 patients (59.4%) had an introverted personality, and 34 (53.1%) had unstable emotions. Thirteen families (19.7%) were uncooperative with the treatment. Patients who had cormorbid anxiety or depression exhibited significantly lower cognitive ability ( P < 0.01). Logistic regression found that better treatment outcomes were positively associated with having a close relationship with parents, parental cooperation with treatment, and having a father with a lower level of education (i.e., less than junior college or higher). Conclusions The characteristics and outcomes of children with dissociative (conversion) disorders are related to socio-cultural environmental variables and psychiatric/psychological factors. Timely recognition and effective treatment of dissociative (conversion) disorders are important.

Conversion disorder

Dissociative disorders

Depression

Biopsychosocial model

Adjustment disorders

10.1186/s12888-021-03045-0

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Citations (9)

Subcellular reorganization and altered phosphorylation of the astrocytic gap junction protein connexin43 in human and experimental temporal lobe epilepsy

Glia (2017)

Tushar Deshpande Tingsong Li Michel K. Herde Albert J. Becker Hartmut Vatter

Dysfunctional astrocytes are increasingly recognized as key players in the development and progression of mesial temporal lobe epilepsy (MTLE). One of the dramatic changes astrocytes undergo in MTLE with hippocampal sclerosis (HS) is loss of gap junction coupling. To further elucidate molecular mechanism(s) underlying this alteration, we assessed expression, cellular localization and phosphorylation status of astrocytic gap junction proteins in human and experimental MTLE-HS. In addition to conventional confocal analysis of immunohistochemical staining we employed expansion microscopy, which allowed visualization of blood-brain-barrier (BBB) associated cellular elements at a sub-µm scale. Western Blot analysis showed that plasma membrane expression of connexin43 (Cx43) and Cx30 were not significantly different in hippocampal specimens with and without sclerosis. However, we observed a pronounced subcellular redistribution of Cx43 toward perivascular endfeet in HS, an effect that was accompanied by increased plaque size. Furthermore, in HS Cx43 was characterized by enhanced C-terminal phosphorylation of sites affecting channel permeability. Prominent albumin immunoreactivity was found in the perivascular space of HS tissue, indicating that BBB damage and consequential albumin extravasation was involved in Cx43 dysregulation. Together, our results suggest that subcellular reorganization and/or abnormal posttranslational processing rather than transcriptional downregulation of astrocytic gap junction proteins account for the loss of coupling reported in human and experimental TLE. The observations of the present study provide new insights into pathological alterations of astrocytes in HS, which may aid in the identification of novel therapeutic targets and development of alternative anti-epileptogenic strategies.

Hippocampal sclerosis

Human brain

Extravasation

10.1002/glia.23196

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Citations (79)

Prognostic factors for the recurrence of afebrile seizures after benign convulsions associated with mild gastroenteritis

Epilepsia (2021)

Duan Wang Yu Jiang Siqi Hong Jiannan Ma Shuang Liao

The overall prognosis of benign convulsions associated with mild gastroenteritis (CwG) is favorable, and the incidence of afebrile seizure recurrence with or without gastroenteritis (ASwGI and ASwoGI, respectively) is low. In this study we investigated the prognostic factors associated with afebrile seizure (AS) relapse after the first CwG episode.A hospital-based cohort with an initial CwG episode from January 2012 to October 2019 was followed for at least 19 months. The relapse types were divided into ASwGI and ASwoGI. Logistic regression analysis was performed to identify the independent prognostic factors for the recurrence of AS after the initial CwG episode. Furthermore, the clinical characteristics between ASwGI and ASwoGI were compared.Among the 868 patients enrolled, 67 (7.7%) experienced a second AS and 71% (48/67) showed gastroenteritis-associated recurrence. Except for five patients with subsequent epilepsy (0.6%), only eight (0.9%) experienced three seizure episodes. The independent predictive factors for the subsequent recurrence of AS were age less than 18 months at onset (odds ratio [OR]: 2.93, 95% confidence interval [CI]: 1.53-5.63), repeated seizures over 24 h (OR: 4.09, 95% CI: 2.19-7.65), and absence of fever (OR: 2.33, 95% CI: 1.26-4.33) during the first CwG episode. The probability of recurrence of AS for those with one, two, and three predictive factors was 3.23%, 13.35%, and 22.85%, respectively. In addition, the age at onset was significantly lower in the ASwoGI group than in the ASwGI group during the first episode (p < .05).The risk of AS relapse after the initial CwG episode is low, and the majority of patients presented with gastroenteritis. The risk can be predicted by age at onset, repeated seizures over 24 h, and absence of fever during the first CwG episode.

Convulsion

10.1111/epi.17102

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Citations (10)

Dynamic Changes of Astrocytes and Adenosine Signaling in Rat Hippocampus in Post-status Epilepticus Model of Epileptogenesis

Cellular and Molecular Neurobiology (2018)

Siqi Hong Tingsong Li Yuanyuan Luo Wenjuan Li Tang Xiao-ju

Adenosine A1 receptor

10.1007/s10571-018-0590-9

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Citations (4)

Prednisolone/prednisone as adrenocorticotropic hormone alternative for infantile spasms: a meta‐analysis of randomized controlled trials

Developmental Medicine & Child Neurology (2020)

Shaojun Li Xuefei Zhong Siqi Hong Tingsong Li Li Jiang

Aim To compare the efficacy and safety of prednisolone/prednisone and adrenocorticotropic hormone (ACTH) in the treatment of infantile spasms using a meta‐analysis of randomized controlled trials (RCTs). Method In a systematic literature search of electronic databases (MEDLINE, Embase, the Cochrane Library), we identified RCTs that assessed prednisolone/prednisone compared with ACTH/tetracosactide in patients with infantile spasms. The electroclinical response and adverse events were evaluated. Results Six RCTs (616 participants) were included in the meta‐analysis. Compared with prednisolone/prednisone, ACTH/tetracosactide was not superior in terms of cessation of spasms at day 14 (relative risk 1.19, 95% confidence interval [CI] 0.74–1.92), day 42 (relative risk 1.02, 95% CI 0.63–1.65), and resolution of hypsarrhythmia on electroencephalogram (relative risk 1.14, 95% CI 0.71–1.81); the incidences of common adverse reactions caused by ACTH/tetracosactide were not lower than that of prednisolone/prednisone for irritability (relative risk 0.79, 95% CI 0.57–1.10), increased appetite (relative risk 0.78, 95% CI 0.57–1.08), weight gain (relative risk 0.86, 95% CI 0.56–1.32), and gastrointestinal upset (relative risk 0.60, 95% CI 0.35–1.02), though it seemed less frequent. Interpretation Prednisolone/prednisone elicits a similar electroclinical response as ACTH for infantile spasms, which indicates that it can be an alternative to ACTH for treating infantile spasms. What this paper adds Prednisolone/prednisone is as effective as adrenocorticotropic hormone (ACTH) in electroclinical response of infantile spasms. Prednisolone/prednisone and ACTH cause similar and tolerable adverse effects, whose incidences are comparable. High‐dose prednisone/prednisolone might be preferable to low dose for achieving freedom from spasms.

Prednisolone

10.1111/dmcn.14452

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Citations (29)

Author response for "Clinical characterization of epilepsy in children with chromosomal aberration 47, XXY"

Congjie Chen Yuanyuan Luo Xueqing Hou Tingsong Li

Characterization

10.1002/brb3.3178/v3/response1

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Refractive Index and Alcohol-Concentration Sensor Based on Fano Phenomenon

Sensors (2022)

Qiang Wang Shubin Yan Jilai Liu Xiaoyu Zhang Lifang Shen

A novel nano-refractive index sensor based on the Fano resonance phenomenon is proposed in this paper. The sensor consists of the metal-insulator-metal (MIM) waveguide and a V-ring cavity with a groove (VRCG). We analyzed the performance of the nanoscale sensor using the finite element method. The simulation results show that the asymmetry of the geometric structure itself is the main factor leading to Fano resonance splitting. In Fano splitting mode, the Fano bandwidth of the system can be significantly reduced when the sensor sensitivity is slightly reduced, so that the figure of merit (FOM) of the sensor can be substantially improved. Based on the above advantages, the sensor's sensitivity in this paper is as high as 2765 nm/RIU, FOM = 50.28. In addition, we further applied the sensor to alcohol concentration detection. The effect is good, and the sensitivity achieves about 150. This type of sensor has a bright future in the precision measurement of solution concentrations.

Fano plane

Phenomenon

10.3390/s22218197

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Astrocytic connexin 43‐hemichannels aggravate seizures by modulating blood‐brain barrier permeability in temporal lobe epilepsy mice

Pediatric Discovery (2024)

Honghong Song Yi Li Jiayu Liu Xianshu Bai Li Jiang

Abstract The involvement of astrocytic connexin 43 (Cx43) in epileptogenesis has been extensively studied through various approaches, yet the underlying mechanism remains enigmatic. In this study, we explored whether astrocytic Cx43 forms hemichannels (HCs) that contribute to seizure progression in temporal lobe epilepsy (TLE) in mice. We focused on how these HCs influence the permeability of the blood‐brain barrier (BBB), a crucial factor in the pathophysiology of epilepsy. Immunofluorescence staining and western blot analysis were employed to assess Cx43 expression in kainic acid‐induced TLE mice, while BBB permeability was evaluated in TLE mice and those treated with TAT‐Gap19 (an astrocytic Cx43 HC inhibitor) using Evans Blue permeation, serum S100β protein quantification, ZO‐1 expression, and albumin extravasation into brain parenchyma via western blotting. Furthermore, seizure burden was monitored continuously using telemetric electroencephalography (EEG) and video monitoring in epileptic and TAT‐Gap19‐treated mice. Results demonstrated a significant increase in Cx43 content in hippocampal tissue in the TLE group, with a pronounced expression around blood vessels. TAT‐GAP19 treatment alleviated EEG seizures and BBB permeability in TLE mice. These findings suggest that astrocytic Cx43 HCs in the hippocampus play a crucial role in epileptogenesis and seizure progression by regulating BBB permeability. Targeting Cx43‐formed HCs distributed around the neurovascular unit may offer a novel therapeutic approach for epilepsy.

Evans Blue

Extravasation

Carbenoxolone

10.1002/pdi3.2500

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