M Enany

Cairo University

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First Report of Zoonotic Endocarditis in Egypt: High Prevalence of Brucella in Culture-Negative Endocarditis

International Journal of Infectious Diseases (2012)

M Enany Hussien Rizk Amani El‐Kholy E. Abdul Rahman Hussein Heshmat

Background: Background:Zoonotic agents, such asCoxiella burnetii,Brucellaspp., andBartonellaspp.were frequently detected in North Africa and identified as frequent causes of infective endocarditis (IE) in some countries. In Egypt, 50% of the patients diagnosed as definite IE are blood culture negative and the medical institutes in Egypt do not include zoonotic agents laboratory diagnosis in the IE workup. Objective: To study the contribution ofBrucella spp., Bartonella spp.andCoxiella burnetiiin IE in Egypt and to study the application of laboratory detection methods for these agents as a routine in the IE workup. Methods: Methods:A prospective study on patients with suspected IE referred to the Cardiology Department of Cairo University from February 2005 to February 2009. Three sets of blood culture were withdrawn on admission. Resected valves were cultured. Sera of patients were tested forBrucellaantibodies using standard agglutination test. IFA test for IgG forBartonella,and IgG, IgM, and IgA forCoxiella burnetiiwas done. PCR was performed on blood of patients with positive serology forBrucella,andon 33 cardiac valves forBartonella. Results: Results:150 patients were diagnosed as definite IE; 50% of them had BCNE. By serology, zoonotic pathogens were identified in 11 of all IE patients;Brucellain 5,Bartonellain 5 andCoxiella burnetiiin 1. PCR forBrucellawas positive for the 5 patients and for one patient withBartonellapositive serology. After the completion of the research, the detection of zoonotic agents was applied as a routine in the IE workup for the first time in Egypt and this approach was also disseminated to other institutes through different educational activities and laboratory cooperation. Conclusion: Conclusion:The study showed a higher proportion of IE due toBrucellathan any other reported in North Africa. The quality of IE patients management in Egypt was improved after completion the this work.

Blood Culture

Bartonella

Direct agglutination test

Infective Endocarditis

Rapid diagnostic test

10.1016/j.ijid.2012.05.523

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Citations (0)

Evaluation of broad-range 16S rRNA PCR for the diagnosis of bloodstream infections: two years of experience

The Journal of Infection in Developing Countries (2014)

Reem Hassan M Enany Hussien Rizk

Diagnosis of bloodstream infections using bacteriological cultures suffers from low sensitivity and reporting delay. Advanced molecular techniques introduced in many laboratories provide rapid results and may show improvements in patient outcomes. This study aimed to evaluate the usefulness of a molecular technique, broad-range 16S rRNA PCR followed by sequencing for the diagnosis of bloodstream infections, compared to blood culture in different patient groups.Conventional PCR was performed, using broad-range 16S rRNA primers, on blood cultures collected from different patients with suspected bloodstream infections; results were compared with those of blood culture.Though blood culture is regarded as the gold standard, PCR evaluation showed sensitivity of 86.25%, specificity of 91.25%, positive predictive value of 76.67%, negative predictive value of 95.22%, and accuracy of 88.8%.Molecular assays seem not to be sufficient to replace microbial cultures in the diagnosis of bloodstream infections, but they can offer a rapid, good negative test to rule out infection due to their high negative predictive value.

Gold standard (test)

Blood Culture

Bloodstream infection

Bacteremia

Microbiological culture

10.3855/jidc.4867

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Citations (16)

P016: Device associated infections in adult intensive care units in public versus private hospitals in Egypt

Antimicrobial Resistance and Infection Control (2013)

M Abdelfattah Amani El‐Kholy M Enany I Beheiry D. Saleh

The healthcare system depends on public hospitals including university hospitals and private hospitals in Egypt. Private hospitals have more resources, and healthcare workers work under strict supervision, monitoring and feed- back.

Medical microbiology

Healthcare worker

10.1186/2047-2994-2-s1-p16

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Citations (2)

P015: Prevention of re-use of single-use suction catheter decreased the vap incidence density in a resource-limited adult ICU in Egypt

Antimicrobial Resistance and Infection Control (2013)

M Enany M. M. Sherif AA El Kholy Guljana Saeed Elham Sobhy

Ventilator-associated pneumonia (VAP) had a major impact on patient morbidity and mortality in our ICU, an adult ICU in a tertiary hospital, in spite of application of ventilator- associated pneumonia prevention (VAP) bundle. Between March 2009 and May 2010 the incidence rate was 17/1,000 device-days

Medical microbiology

10.1186/2047-2994-2-s1-p15

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Citations (4)