Abstract Background: Previous studies have demonstrated the benefits of posterior lumbar spine fusion with orthotopic paraspinal muscle-pediculated bone flaps. However, non-union due to poor mechanical support remains a concern. Flexible devices, such as PEEK, are superior to conventional rigid devices in facilitating union and reducing adjacent segmental movement. Here, we assessed whether PEEK rods can accelerate fusion and reduce adjacent segmental movement to prevent adjacent segment disease. Methods: Thirty-two mongrel dogs were assigned randomly into two groups for lumbar fusion surgery: titanium rods (control group; n =16); PEEK rods (treatment group; n =16). Animals were killed 8 or 16 weeks after surgery. The lumbar spines were harvested, and spinal changes were analyzed by manual palpation, gross examination, 3D motion tests, 3D-CT reconstruction (for rate of fusion), and µCT (for 3D structures of the fusion masses). Immunohistochemistry was used to examine the expression of BMP-2 and VEGF. Results: The results indicated no significant differences in the lumbar spine of the control and treatment groups in terms of manual palpation, gross examination, 3D-CT reconstruction or range of movement ( P >0.05). However, BMP-2 and VEGF expression was higher in the treatment group than in the control group at 8 weeks (BMP-2, 106.7 ± 8.8 vs. 90.4 ± 9.6, respectively, P <0.05; VEGF: 71.1 ± 10.6 vs. 58.5 ± 12.4, respectively; P <0.05). Results from the µCT showed better 3D structures of the fusion masses and higher bone quality in the treatment group ( P <0.05). Conclusion: PEEK rods increase the rate and quality of fusion but provide a similar range of adjacent segment movement as titanium rods.
The ABO blood group system is the most important blood type system in human transfusion medicine. Here, we explore the specificity of antibody recognition towards ABO blood group antigens using computational modeling and biolayer interferometry. Automated docking and molecular dynamics (MD) simulations were used to explore the origin of the specificity of an anti-blood group A antibody variable fragment (Fv AC1001). The analysis predicts a number of Fv-antigen interactions that contribute to affinity, including a hydrogen bond between a HisL49 and the carbonyl moiety of the GalNAc in antigen A. This interaction was consistent with the dependence of affinity on pH, as measured experimentally; at lower pH there is an increase in binding affinity. Binding energy calculations provide unique insight into the origin of interaction energies at a per-residue level in both the scFv and the trisaccharide antigen. The calculations indicate that while the antibody can accommodate both blood group A and B antigens in its combining site, the A antigen is preferred by approximately 4 kcal/mol, consistent with the lack of binding observed for the B antigen.
Purpose: To explore the effects of autogenous bone particle/titanium fiber composites on repairing segmental bone defects in rabbits. Materials and methods: A model of bilateral radial bone defect was established in 36 New Zealand white rabbits which were randomly divided into 3 groups according to filling materials used for bilaterally defect treatment: in group C, 9 animal bone defect areas were prepared into simple bilateral radius bone defect (empty sham) as the control group; 27 rabbits were used in groups ABP and ABP-Ti. In group ABP, left defects were simply implanted with autogenous bone particles; meanwhile, group ABP-Ti animals had right defects implanted with autogenous bone particle/titanium fiber composites. Animals were sacrificed at 4, 8, and 12 weeks, respectively, after operation. Results: Micro-CT showed that group C could not complete bone regeneration. Bone volume to tissue volume values in group ABP-Ti were better than group ABP. From histology and histomorphometry Groups ABP and ABP-Ti achieved bone repair, the bone formation of group ABP-Ti was better. The mechanical strength of group ABP-Ti was superior to that of other groups. Conclusions: These results confirmed the effectiveness of autologous bone particle/titanium fiber composites for promoting bone regeneration and mechanical strength.
The double burden of diabetes mellitus (DM) and tuberculosis (TB) has attracted increasing attention, because DM not only increases the risk of active TB but also affects treatment outcomes. Screening for TB among diabetic patients has been recommended, but requires real-world evidence by considering its cost-effectiveness, cost-utility ratio, and cost-benefit ratio.A screening program was conducted in Jiangyin City of Jiangsu Province, China. A total of 14 869 diabetic patients received regular physical examinations for three consecutive years and were followed for the diagnosis of TB. The cost of screening and the effectiveness, utility, and social benefits attributed to the program were evaluated. In addition, a matched case-control study was conducted and the nomogram was used to identify high-risk groups that could be the target population for screening.Among the 14 869 diabetic patients who participated in this screening program, 22 were diagnosed with TB, resulting in an incremental cost-effectiveness ratio (ICER) of 83 910 CNY per disability-adjusted life-year (DALY) gained and a cost-benefit ratio of 0.50. If the screening program was limited to high-risk diabetic patients by considering body mass index (BMI), fasting blood glucose (FBG), and triglycerides, the ICER decreased to 34 303 CNY per DALY gained and the cost-benefit ratio increased to 1.22.Screening for TB using regular chest X-ray examinations is feasible but not economical in areas with a low incidence of TB. It is recommended that diabetic patients with a low BMI, high FBG, and low triglycerides are selected as subjects for TB screening.
Objective To observe the therapeutic effect of Canggezhixieling on infantile rotavirus enteritis.Methods 124 patients with infantile rotavirus enteritis were randomly divided into two groups.In treatment group(n=74)Canggezhixieling was orally taken;in control group(n=50) smecta was orally taken.Results The cure rate was 86.49% in treatment group and 50.00% in control group,and there was a significant difference between two groups (P0.01). Meantime the improvement of clinical symptoms in treatment group was superior to that in control group.Conclusion Canggezhixieling has definite therapeutic effect on infantile rotavirus enteritis.
The persistent primitive hypoglossal artery (PPHA) is the second most common persistent carotid-vertebrobasilar anastomosis. We present a rare case of an 82-year-old woman who diagnosed as acute cerebral infarction three times in both the anterior and posterior circulation territories with a right-sided PPHA and ipsilateral carotid artery dissection (CAD). Microembolus monitoring results suggested that microembolic caused by CAD associated with PPHA should be recognized as a possible cause of multiple infarctions in both the anterior and posterior circulation territories. For these patients, appropriate treatment measures should be taken for prevention of stroke recurrence.
To study the clinical efficacy and safety of dexamethasone of different doses combined with bortezomib and thalidomide for treatment of primary multiple myeloma.Ninety-six patients with multiple myeloma from January 2013 to January 2014 were randomly divided into group A (high-dose dexamethasone + bortezomib + thalidomide, 32 cases), group B (low-dose dexamethasone + bortezomib + thalidomide, 32 cases) and group C (placebo + bortezomib + thalidomide, 32 cases). The clinical efficacy and safety of patients was compared among 3 groups.The overall remission rate (ORR) in group A and B was significantly higher than that in group C (P<0.05), but the ORR was not significant difference between group A and group B (P>0.05). After treatment, the KPS and RNS score in 3 groups were significantly higher and lower than those before treatment, respectively; the KPS score in group A and B was significantly higher than that in group C (P<0.05), the RNS score in group A and B was significantly lower C (P<0.05). After treatment, the positive expression rates of CD38, CD56 and CD138 as well as small residual lesion (SRL) positive rate in 3 grops were significantly lower than those before treatment, but the positive expression rate of CD19 was significantly higher that before treatment; the positive expression rates of CD38, CD56 and CD138 as well as SRL positive rate in group A and B were significantly lower thant those in group C, while the positive expression rate of CD19 was significantly higher that in group C (P<0.05), but the positive expression rates of CD19, CD38, CD56 and CD138 as well as SRL positive rate were not significantly different between group A and B (P>0.05). The incidence of fatigue, rash, peripheral neuropathy, anlmia, granulocyte deficiance and so on in group B and C was significantly lower than that in group A(P<0.05), but the difference in group B and C was not significant (P>0.05).The therapeutic efficacy of different doses of dexamethasone combined with bortezomib and thalidomide for patients with multiple myeloma is similar, can obviously enhance remission rate, prolong the survival time, promote life quality, but the incidence of adverse reactions in low dose dexamethason rigemen is significantly reduced, and the safety is better.
'/%3e%3cuse xlink:href='%23a' transform='rotate(23.036 .093 25.536)'/%3e%3cuse xlink:href='%23a' transform='rotate(45.87 1.273 16.18)'/%3e%3cuse xlink:href='%23a' transform='rotate(69.945 .996 12.078)'/%3e%3cuse xlink:href='%23a' transform='rotate(20.66 -19.689 31.932)'/%3e%3c/svg%3e)