David A. Gutierrez

University of California, Davis

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Jared T. Shaw

University of California, Davis

James C. Fettinger

University of California, Davis

Kaori Ando

Osaka University

Garrett Toth-Williams

University of California, Davis

K. N. Houk

University of California, Los Angeles

Anna Lo

University of California, Davis

Jie Jack Li

Jinggangshan University

M. Tonezzer

University of Trento

A. Quaranta

Istituto Nazionale di Fisica Nucleare, Trento Institute for Fundamental Physics And Applications

Ana Luisa Muñoz

Universidad Antonio Nariño

Cooperative Institutions

Universidad Antonio Nariño

University of California, Davis

Pfizer (United States)

Institute for Myeloma & Bone Cancer Research

University of California, Berkeley

University of San Francisco

Rensselaer Polytechnic Institute

University of British Columbia

University of Nebraska–Lincoln

Howard Hughes Medical Institute

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Improving solar cell efficiency with novel luminescent thin films

M. Tonezzer G. Maggioni Enrico Menin S. Carturan Mónica Della Pirriera

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Salud bucodental para comunidades vulnerables y desplazadas de Popayán que viven en condiciones de pobreza

Revista Cubana de Salud Pública (2019)

Inés Amparo Revelo Mejía David A. Gutierrez Claudia Natalí Castro Garcés Luis Fernando Rodríguez Muñoz

Introduccion: En el Municipio de Popayan, departamento del Cauca, Colombia, hay mas de 23 asentamientos, donde viven cerca de 4 mil personas desplazadas en condicion de vulnerabilidad y pobreza, quienes, en su mayoria, no estan protegidos por el del sistema de salud y carecen de atencion odontologica. Objetivo: Aplicar los conocimientos sobre salud oral adquiridos en la carrera de odontologia, para mejorar la salud bucodental en comunidades vulnerables y desplazadas que viven en condiciones de pobreza en Popayan, Cauca, Colombia. Metodo: Estudio cualitativo, tipo investigacion accion. La poblacion beneficiada se conformo por 1515 personas, a las que se le aplicaron procedimientos educativos, preventivos, curativos y restaurativos, durante 5 anos. Resultados: La poblacion mas beneficiada fue la vulnerable; los mas atendidos fueron: en cuanto a genero. el femenino; rango de edad, ninos entre 3 y 8 anos y adultos, de 36 y mas anos. El procedimiento odontologico que mas se realizo en ninos fue la profilaxis dental y en adultos el raspaje supragingival. Conclusiones: Aplicar el curriculo de la carrera de Odontologia centrado en la promocion de salud y en el aprendizaje contextualizado a partir de su aplicacion en virtud del desarrollo de la comunidad, lo convierte en un curriculo con mayor compromiso social. Y la comunidad que recibe los beneficios se convierte en ente activo de los procesos de promocion de salud oral. La poblacion vulnerable y la desplazada manifiestan receptividad ante los tratamientos clinicos orales que se le ofrecen.

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Liquefaction experiment and analysis projects (LEAP): Summary of observations from the planning phase

Soil Dynamics and Earthquake Engineering (2017)

Majid T. Manzari Mohamed El Ghoraiby Bruce L. Kutter Mourad Zeghal Tarek Abdoun

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Base (topology)

10.1016/j.soildyn.2017.05.015

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Citations (72)

Análisis estructural de la fibrina rica en plaquetas y sus aplicaciones en odontología regenerativa

Universitas Odontologica (2018)

David A. Gutierrez Juan Pablo Hinojosa Sarria Ana Isabel Restrepo Dorado Ana Luisa Muñoz Néstor Velarde

Antecedentes: La fibrina rica en plaquetas (PRF) es un concentrado plaquetario que se está usando con mayor frecuencia en medicina y odontología. Los resultados clínicos son variables posiblemente porque hay diferentes protocolos de obtención, equipos de centrifugado y técnicas de colocación. El desconocimiento de los aspectos estructurales puede afectar el resultado clínico. Objetivo: Describir las características estructurales de la PRF en las diferentes zonas de la membrana. Métodos: Se realizó un estudio experimental in vitro con 15 muestras de sangre periférica tomada de cinco voluntarios adultos, sanos, asistentes a la clínica odontológica de la Universidad Antonio, Popayán. Se hizo hemograma inicial, se recolectó sangre y se centrifugó (10 min x 3000 rpm). Las muestras se analizaron histológicamente y con microscopía electrónica de barrido (SEM). Se describió la estructura de la fibrina, las plaquetas y los leucocitos. Resultados: El promedio de recuento de plaquetas en sangre total fue de 251±31,74 x103 x mm3 y en PRF fue de 832±123,43 x103 x mm3. Macroscópicamente, se identificaron tres zonas del PRF: una superior con pocas plaquetas, una zona leucocitaria (BC) y una zona corpuscular roja. En el análisis de microscopía óptica muestra que en la zona BC hay mayor concentración plaquetaria. El análisis por SEM comprueba que la estructura de la red de fibrina y el contenido celular son diferenciales en cada zona. Conclusión: A partir del conocimiento estructural del PRF se pueden proponer aplicaciones que mejoren el rendimiento del material y por tanto los resultados clínicos.

10.11144/javeriana.uo37-79.aefr

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PO-54: Preliminary evaluation of venous thromboembolic risk in patients with colorectal cancer from the CARTAGO study

Thrombosis Research (2022)

M. Salgado R. Molina E. Brozos Vázquez E. Martínez E. Cillán

10.1016/s0049-3848(22)00244-4

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Synergistic Anti-Myeloma Effects of the Lyn Kinase Inhibitor INNO-406 In Combination with Doxorubicin, Melphalan and Bortezomib

Blood (2010)

Zhiwei Li Eric Sanchez Cathy Wang Mingjie Li David A. Gutierrez

LYN

10.1182/blood.v116.21.5015.5015

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CCDC 2114719: Experimental Crystal Structure Determination

The Cambridge Structural Database (2022)

Anna Lo David A. Gutierrez Garrett Toth-Williams James C. Fettinger Jared T. Shaw

10.5517/ccdc.csd.cc28zjrk

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Effects of 970 nm diode laser irradiation on morphology, proliferation, and differentiation of gingival mesenchymal stem cells

Journal Of Oral Research (2023)

Javiera Ortiz Fernando Matamala Catherine Andrade David A. Gutierrez Carolina Inostroza

Objective: The objective of this study was to investigate the morphology, proliferation, and differentiation of gingival mesenchymal stem cells (GMSCs) irradiated with a 970 nm Diode Laser (LLLT). It is essential to validate the efficacy of treatment, optimize irradiation conditions and guarantee the safety and quality of stem cells for future use in dental applications. Materials and Methods: GMSCs were cultured in standard conditions and irradiated with a Diode laser (970 nm, 0.5W) with an energy density of 9J/cm2. Cell proliferation was assessed with the WST-1 proliferation kit. GMSCs were differentiated into chondrogenic and osteogenic lineages. Cell morphology was performed with Hematoxylin/eosin staining, and quantitative nuclear analysis was done. Cell viability was monitored with trypan blue testing. Results: GMSCs subjected to irradiation demonstrated a significant increase in proliferation at 72 hours compared to the non-irradiated controls (p=0.027). This indicates that the 970 nm diode laser has a stimulatory effect on the proliferation of GMSCs. LLLT-stimulated GMSCs exhibited the ability to differentiate into chondrogenic and osteogenic lineages. A substantial decrease in cell viability was observed 24 hours after irradiation (p=0.024). However, after 48 hours, the cell viability recovered without any significant differences. This indicates that there might be a temporary negative impact on cell viability immediately following irradiation, but the cells were able to recover and regain their viability over time. Conclusions: This study support that irradiation with a 970 nm diode laser could stimulate the proliferation of GMSCs, maintain their ability to differentiate into chondrogenic and osteogenic lineages, and has minimal impact on the mor- phological characteristics of the cells. These results support the potential use of NIR Lasers in combination with GMSCs as a promising strategy for dental treatments. Keywords: Photobiomodulation; semiconductor lasers; Mesenchymal stem cells; Gingiva; Cell Proliferation; Safety.

Morphology

10.17126/joralres.2023.008

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Supporting the potential of quantitative ultrasonic techniques for the evaluation of platelet concentration

Edgar Gutierrez Tatiana Molano Yady M. Jiménez Julián Antonio Villamarín Luis Fernando Londoño Franco

This article describes the results obtained by making use of a non-destructive, non-invasive ultrasonic system for the acoustic characterization of bovine plasma rich in platelets using digital signal processing techniques. This study includes computational methods based on acoustic spectrometry estimation and experimental measurements of the speed of sound in blood plasma from different samples analyzed, using an ultrasonic field with resonance frequency of 5 MHz. The results showed that the measurements on ultrasonic signals can contribute to the hematological predictions based on the linear regression model applied to the relationship between experimental ultrasonic parameters calculated and platelet concentration, indicating a growth rate of 1 m/s for each 0.90 x10³ platelet per mm³. On the other hand, the attenuation coefficient presented changes of 20% in the platelet concentration using a resolution of 0.057 dB/cm MHz.

SIGNAL (programming language)

10.1117/12.2288118

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Osteogenic potential of apical papilla stem cells mediated by platelet-rich fibrin and low-level laser

Odontology (2023)

David A. Gutierrez Carolina Inostroza Mahmoud Rouabhia Camilo Alfonso Rodriguez Lina Andrea Gómez

Abstract To evaluate the osteogenic potential of platelet-rich fibrin (PRF) and low-level laser therapy (LLLT) on human stem cells from the apical papilla (SCAP) we isolated, characterized, and then cultured in an osteogenic medium cells with PRF and/or LLLT (660 nm, 6 J/m2-irradiation). Osteogenic differentiation was assessed by bone nodule formation and expression of bone morphogenetic proteins (BMP-2 and BMP-4), whereas the molecular mechanisms were achieved by qRT-PCR and RNA-seq analysis. Statistical analysis was performed by ANOVA and Tukey’s post hoc tests ( p < 0.05* and p < 0.01**). Although PRF and LLLT increased bone nodule formation after 7 days and peaked at 21 days, the combination of PRF + LLLT led to the uppermost nodule formation. This was supported by increased levels of BMP-2 and -4 osteogenic proteins ( p < 0.005). Furthermore, the PRF + LLLT relative expression of specific genes involved in osteogenesis, such as osteocalcin, was 2.4- ( p = 0.03) and 28.3- ( p = 0.001) fold higher compared to the PRF and LLLT groups, and osteopontin was 22.9- and 1.23-fold higher, respectively ( p < 0.05), after 7 days of interaction. The transcriptomic profile revealed that the combination of PRF + LLLT induces MSX1, TGFB1, and SMAD1 expression, after 21 days of osteogenic differentiation conditions exposition. More studies are required to understand the complete cellular and molecular mechanisms of PRF plus LLLT on stem cells. Overall, we demonstrated for the first time that the combination of PRF and LLLT would be an excellent therapeutic tool that can be employed for dental, oral, and craniofacial repair and other tissue engineering applications.

Platelet-rich fibrin

Osteopontin

10.1007/s10266-023-00851-8

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Citations (5)