Pla Medical

Chinese PLA General Hospital

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Xiaopeng Lan

Affiliated Hospital of Qingdao University

Kan Liu

Second Affiliated Hospital of Nanchang University

Wang Wei

Fuyang Second People's Hospital

Yuyan Zhou

Changhai Hospital

Zhu Dong-ping

Technical Institute of Physics and Chemistry

Fang Chi-hu

Zhujiang Hospital

Li Juan Song

Building Research Institute

Yang Xiang

Nanchang University

LU Ke-fu

Second Military Medical University

Zhang Hui-j

Cooperative Institutions

Chinese Academy of Sciences

Army Medical University

Second Military Medical University

Nanchang University

Nanjing University

Jilin University

Fuzhou General Hospital of Nanjing Military Command

Second Affiliated Hospital of Nanchang University

Chinese PLA General Hospital

Washington University in St. Louis

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A prospective study of the development of the information system in China's hospitals

Zhonghua yiyuan guanli zazhi (1997)

Pla Medical

The popularization of computer hardware and software technique and knowledge and the introduction of computers and the special attention paid to their application provide favorable conditions for the development of information systems in China's hospitals. At present, the computer system used by China's hospitals are limited in coverage, their functions incomplete, their models backward and their formation into a national network difficult. Therefore, more work should be done to uniform the software and hardware systems, to establish multimedia electronic medical records and to set down regulation on service payments. A hospital information system with automatic digit and quality control should be the target to hit. Relevant rules and laws ought to be written. The application of computer knowledge and technique should be extensively popularized.

Prospective payment system

Hospital information system

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Sex hormones change of renovascular hypertension rats and effect of Laonianjiangyachongji on sex hormones

Pla Medical

Objective: It is to observe the relation between the changes of estradiol (E 2) and testosterone (T) levels in plasma of the renovascular hypertension rat (RHR) and hypertension, and the effect of Laonianjiangyachongji (LNJYCHJ) on the sex hormones. Methods: The RHR models were made and randomly divided into five groups. The changes of E 2 and T levels in plasma were observed. Results: T level in plasma decreased significantly ( P 0.01), but E 2 level in plasma increased significantly ( P 0.01). LNJYCHJ could increase the density of T in plasma, while it could decrease the density of E 2 in plasma. Conclusion: The T and E 2 might have important role in the hypertension form of the RHR. The regulation of T and E 2 might be one of the anti hypertension mechanisms of LNJYCHJ.

Renovascular Hypertension

Plasma levels

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VERTICAL DISTRIBUTION OF AEDES ALPOPICTUS AND COMPOSITION OF MOSQUITO POPULATIONS IN BAMBOO FOREST OF WUYISHAN NATURE RESERVE,FUJIAN PROVINCE (DIPTERA:CULICIDAE)

Wuyi kexue (1985)

Pla Medical

Four bamboo forests at different altitudes in Wuyi mountain were sur veyed for the presence of Aedes albopictus from July to August in 1984.This mosquito was found in residential area from low altitude up to 800m above sea level.It was not found in bamboo forest in inhabited region at higher altitude(950-1250m). Larvae of 6 genera and 16 species of mosquitoes had been collected from bamboo-holes,among which Aedes fengi was the dominant one.It occurred in 602 out of 828 bamboo-holes examined,with a frequency of 72.71%.

Aedes albopictus

Low altitude

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A Study of Methodology of Library and Information Science in the 21st Century in China

Pla Medical

According to the classification for the methodology of library and information Science put forward by Professor Wang Chongde,the article introduces new development in China during nearly 10 years from the three aspects of the philosophical method,general methods and specific methods.It also points out the existing problems such as the lack of empirical study,the lack of new methods for the network environment.It proposes that the empirical research and standardization development should be strengthened;research methods for information technology environment should be established;a stable,unified,standardized methodology system should be established.

Empirical Research

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Experimental Research of Distribution and Migration of Oval Cells in Progressive Hepatic Injury

Zhongguo pu-wai jichu yu linchuang zazhi (2004)

Pla Medical

Objective To explore the distribution and migration of oval cells in progressive hepatic injury.Methods Sixty SD rats were divided into the control group ( n =20) and experimental group ( n =40). After the establishment of hepatic carcinoma models, C kit was continuously detected by immunohistochemistry and the liver pathologic changes were regularly observed by optical microscopy. Results The hepatic surface was smooth with eumorphism in histology in the control group. The C kit positive cells were occasionally found. In the experimental group, the oval cells with C kit positive were initially discovered in the portal regions in the second week, and these cells proliferated along the bile duct epithelia. With the hepatic injury becoming more serious, the oval cells extended into the hepatic lobular regions from the portal regions. When hepatocellular carcinoma occurred,the majority were mixed carcinomas, and the oval cells were found inside and outside the carcinoma nodes. In this period, the most of C kit positive cells still located in the portal regions. Conclusion ①The oval cells are the most sensitive cells for the hepatic injury. ②The oval cells which migrate unruly participate in the formation of hepatic pseudolobules. ③The oval cells play an important role in hepatocarcinogenesis.

Hepatic carcinoma

Histology

Hepatic stellate cell

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Experience of clinical application of cholangioscopy in biliary disease:Report of 369 cases

Laboratory Medicine and Clinic (2009)

Yuyan Zhou Pla Medical

Objective To study the clinical application of cholangioscopy in biliary disease.Methods From December 2007 to May 2009,369 cases of patients for check-up and treatment of biliary disease via cholangioscopy during and after surgical interventions were admitted into our hospital.Results The satisfactory effects have been achieved at the diagnosis of biliary disease and treatment of bile duct stones.Conclusion Application of cholangioscopy used in the surgery of bile duct exploration enhances the diagnosis rate of biliary disease and the extraction rate of bile duct stones via the way of surgery,and also avoids the aches of a second surgery for the patient and cut down the cost of treatment.Therefore,we recommend the exploratory and treatment via cholangioscopy be used in the bile duct surgery.

Biliary disease

Bile Duct Diseases

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An investigative analysis on the idleness of large medical equipments

Zhonghua yiyuan guanli zazhi (1997)

Pla Medical

An investigation on the use of medical equipment in 3 hospitals indicates that the introduction of advanced medical equipments has become an important step for hospitals to take part in market competition. But their idleness has become a serious management problem whose solution lies in: ①the necessity of their purchase must be repeatedly proved beforehand and finally referred to the leading department for approval; ②the full use of the medical equipments are to be shared by the hospital community through a jointly made plan; and ③the training of the equipment crew should be expedited to meet the need of the ever renewing advanced medical eqipments.

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A NEW RAPID DETECTION SYSTEM FOR B. ANTHRACIS BASED ON STRING OF PEARLS TEST

ACTA MICROBIOLOGICA SINICA (1981)

Pla Medical

A new rapid detection system for B. anthracis has been developed. It's main procedures read as follows: 1. Cultivate spore containing specimen in rabbit serum tryptose broth for rapid enrichment; 2. Add penicillin (0.5μ/ml) and incubate in 37℃ water bath for 1 hour; 3. Fix the string of pearls and disinfect living cells with formalin; 4. Detect string of pearls of B. anthracis directly by microscopy of wet smear; 5. Stain string of pearls by fluorescent precipitin antibody for further identification, if necessary.According to the results of the experiments of 231 strains of B. anthracis and 164 strains of other aerobic sporeforming bacillus, the detection of artificial contaminated specimens and specimens collected from epizootic area, it has been shown that this detection system has a higher specificity and sensitivity. The correlation rates between routine culture method and this system are 80—90% in the detection of 540 epizootic specimens. It may have been finished within 6—8 hours since the pure culture procedure was omitted, and may be performed in any bacteriological laboratories.It should be pointed out that the positive result may not be got, if the specimen is heavily contaminated by other aerobic sporeforming bacilli, especially by B. cereus or the detected B. anthracis is a penicillin resistant variant.

Bacillus anthracis

Epizootic

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Eukaryotic Expression and Primarily Application of Human Smith D1 Antigen in Methylotrophic Yeast Pichia pastoris

Microbiology (2009)

Yang Xiang Pla Medical

To clone, express and primarily use human autoantigen Sm D1 in methylotrophic yeast Pichia Pastoris. The gene Sm D1 was cloned by PCR.The PCR product was inserted into the vector pPIC9k. The recombinant plasmid pPIC9k- Sm D1 was transformed into yeast SMD1168 by electroporation. The positive clones were screened in MD plates. The high copy number transformants were rapidly selected by using G418 and were induced by methanol. Supernatants after induction were analyzed by SDS-PAGE and im-munodot. The PCR product was showed about 360 bp in size which was in accordance with predicted. The pPIC9k-Sm D1 showed the same seqencing result with GenBank’s report and restriction enzyme analysis confirmed our prediction. The pPIC9k-Sm D1 positive clone produced an about 16 kD protein which had natural immunogenicity of human autoantigen Sm D1 by SDS-PAGE and immunodot. The sensitivity and specificity of immunodot were 96% and 100%, respectively. The agreement between immunodot and im-munoblot was 98%. Successfully cloning and high-level expression of human autoantigen Sm D1 in methy-lotrophic yeast Pichia pastoris laid a foundation for further research work.

clone (Java method)

Pichia

Cloning (programming)

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Application of case teaching in community nursing teaching

Chinese Nursing Research (2006)

Pla Medical

Community nursing

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