Tamaš Petrović

Scientific Veterinary Institute "Novi Sad"

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Sava Lazić

Scientific Veterinary Institute "Novi Sad"

Diana Lupulović

Scientific Veterinary Institute "Novi Sad"

Jasna Prodanov-Radulović

Scientific Veterinary Institute "Novi Sad"

Gospava Lazić

Scientific Veterinary Institute "Novi Sad"

Vladimir Polaček

Scientific Veterinary Institute "Novi Sad"

Dejan Vidanović

Arheološki Institut

Radoslav Došen

Scientific Veterinary Institute "Novi Sad"

Dejan Bugarski

Scientific Veterinary Institute "Novi Sad"

Dušan Petrić

University of Novi Sad

Vesna Milošević

University of Novi Sad

Cooperative Institutions

University of Belgrade

144

University of Novi Sad

131

Scientific Veterinary Institute "Novi Sad"

Croatian Veterinary Institute

University of Veterinary Medicine

University of Zagreb

Institute of Public Health of Vojvodina

Oncology Institute of Vojvodina

IFC Research (United Kingdom)

Institut za Reumatologiju

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Complete Genome Sequence of an Equine Arteritis Virus Strain Isolated from a Lipizzaner Stallion in 2015 in Serbia

Microbiology Resource Announcements (2019)

Delphine Gaudaire Sava Lazić Diana Lupulović Tamaš Petrović Gospava Lazić

Here, we report the first whole-genome sequence of an equine arteritis virus (EAV) strain, RS1, isolated from the semen of a Lipizzaner stallion held in the Vojvodina region of Serbia.

Strain (injury)

Arteritis

Sequence (biology)

Virus strain

10.1128/mra.00250-19

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Citations (1)

Biosecurity measures in villages and rural households.

Radoslav Došen Jasna Prodanov-Radulović Ivan Pušić Mladen Gagrčin Tamaš Petrović

Biosecurity

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Examination of enzymes concentration in the blood of rats with sepsis caused by mixed and pure bacterial cultures

Acta veterinaria (2013)

Dragica Stojanović Zorana Kovačević Marina Žekić Jelena Ašanin Radomir Ratajac

A clinical form of sepsis induced by cecal ligation and puncture (CLP) was caused in order to monitor the concentration of enzymes (alanine aminotransferase - ALT, aspartate aminotransferase - AST, lactate dehydrogenase - LDH, amylase and creatine kinase - CK) in the rat blood. Experiments were performed on male Wistar rats, weighing on average 215±25 g. The rats were divided into four groups. In the first three groups (n=28 per group), sepsis was induced by pure culture of Escherichia coli (EC) or Staphylococcus aureus (SA) and mixed culture (MK) of caecum, while the fourth group included 20 control rats who underwent an abdominal incision. Blood was taken in time intervals of 12, 24, 72 and 120 hours. During the experimental protocol, we identified significant changes of all monitored enzymes in the serum of infected rats. After a period of 12 hours there was a significant increase in ALT (all rats with sepsis), AST and LDH (rats in the MK group) levels, while a decrease was noted in the concentration of amylase (EC, SA). Similarly, 24 hours after the CLP procedure, a significant decrease of amylase (MK) and AST (SA) was recorded, while serum LDH level varied significantly from elevated (EC, SA) to reduced (MC) values. Finally, at the time intervals of 72 and 120 hours the concentration of nearly all monitored enzymes has shown a decline, while significance was noted in lowering of ALT (MK), AST (SA, EC) and amylase (SA) levels. Statistical significance could not be observed in the change of CK levels at any of examined time points.

Creatine kinase

Alanine aminotransferase

10.2298/avb1306609s

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Detection of antibodies against Lumpy skin disease virus by Virus neutralization test and ELISA methods

Acta veterinaria (2019)

Milena Samojlović Vladimir Polaček Vladimir Gurjanov Diana Lupulović Gospava Lazić

Abstract Infection of cattle with lumpy skin disease virus (LSDV) is very important from the aspect of livestock production. Although it can cause significant economic losses, available serological assays are still not sufficiently efficient and reliable. A 3-day VNT was performed using Madin-Darby bovine kidney (MDBK) cell line and LSDV isolated from clinically infected cow to improve serological diagnostics of lumpy skin disease (LSD). In total, 325 cattle sera samples were tested in order to compare the performances of VNT and ELISA. Tested samples originated from 125 cows before the occurrence of LSD in the Republic of Serbia and 200 tested samples originated from vaccinated cows. Sera samples from vaccinated cows were collected starting from the vaccination day to 4 months after vaccination. In 7 different time intervals after vaccination sampling was carried out in 20 cows originating from one herd and in 3 different time intervals in 20 cows originating from a different herd each time of sampling. Out of 200 samples from vaccinated cows, antibodies against LSDV were detected in 68 (34%) samples by VNT, and in 60 (30%) samples by ELISA. No positive finding was detected by VNT in samples collected before the occurrence of LSD in Serbia, while one positive finding was detected in the same samples by ELISA. The first presence of antibodies in vaccinated cattle was detected by both tests 20 days after vaccination, and the largest number of animals with antibodies against LSDV was detected 30 days after vaccination. Comparing the results obtained by VNT and ELISA, it was calculated that kappa index was 0.913. The specificity of VNT and ELISA was 100% and 99.2%, respectively. VNT is simpler to perform compared to the recommended virus neutralization test by the OIE and can improve LSD serological diagnostics with additional sensitivity testing.

10.2478/acve-2019-0003

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Citations (35)

Biological properties of a naturally attenuated infectious bursal disease virus isolated from a backyard chicken flock

Acta Veterinaria Hungarica (2010)

Maja Velhner Darko Mitevski D Potkonjak Dragica Stojanović Mira Kovačević

The biological properties of an infectious bursal disease (IBD) virus isolated from bursas collected during an outbreak in a village chicken flock in Macedonia are described. The mortality rate was 50%. Two viruses coexisted in the bursas of infected chickens (IBDVwt and IBDVtc). The virus termed IBDVtc grows on chicken embryo fibroblast (CEF) cells from the first passage. Specific pathogen free chickens inoculated with IBDVtc at passage level 4 did not develop any clinical signs of disease. Some discrete bleeding on the leg muscles was seen and the bursa of Fabricius revealed pathological lesions similar to those caused by classical strains. However, the bursa recovered quickly (bursa lesion score 2) by 14 days post infection (PI). We also found evidence of bursal repopulation by means of perinuclear antigen staining. Strong CD3 influx was evident at 4 days PI, and at 33 days PI the CD3+ cell finding was comparable to the control. The mean antibody titre was 9.2 log 2 at 14 days PI. The amino acid composition of VP2 in IBDVwt (222 Ala, 242 Ile, 253 Gln, 256 Ile, 279 Asp, 284 Ala, 294 Ile and 299 Ser) is described. The same sequence was found in IBDVtc, except for two point mutations, at Gln253→His and Ala284→Thr. Such amino acid substitution is responsible for partial attenuation and the ability of the strain to replicate in cell culture. None of the commercial vaccine viruses has a similar arrangement of amino acids in the variable domain of IBDV. This strongly suggests that IBDVtc originates from a very virulent strain. To the best of our knowledge, this is the first report of a concomitant infection of chickens with highly pathogenic IBDV and its mutant counterpart.

Infectious bursal disease

Bursa of Fabricius

Flock

Specific-pathogen-free

10.1556/avet.58.2010.4.10

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First Detection and Clinical Presentation of Porcine Epidemic Diarrhea Virus (Pedv) in Serbia

Acta veterinaria (2017)

Jasna Prodanov-Radulović Tamaš Petrović Diana Lupulović Doroteja Marčić Jelena Petrović

Abstract During 2015/2016, fecal and intestinal samples from live diseased and/or dead pigs with suspected PEDV and/or TGEV and signs of rotavirus infection were collected from in total seven different farrow-to-finish swine farms located in Northern Serbia region (Vojvodina Province). A total of 14 samples (2 pools per farm) of small intestine with fecal content were submitted to laboratory molecular investigation (multiplex RTPCR). On these farms the clinical signs included the occurrence of diarrhea in suckling and weaned piglets, with weak or no response to the applied antimicrobial therapy. The epidemic of severe diarrhea affecting pigs of all ages on one farrow-to finish swine farm was detected in January 2016. Watery diarrhea in all swine categories was associated with vomiting and a reduction in feed consumption. Diarrheic, gaunt and dehydrated piglets, covered with feces were found in 90% litters. The disease affected most severely the suckling piglets, and the mortality in newborn piglets was up to 35%. In the weaned piglets and fatteners the mortality was up to 2.5% and 1.2%, respectively. The PEDV RNA was detected in pooled feces and samples of small intestines derived from diseased and dead suckling piglets from only one investigated farm. The PEDV positive samples showed to be negative for rotavirus group A and TGEV. The transport vehicles were identified as the main possible route of PEDV introduction. This is the first report demonstrating the presence of PEDV in Serbia.

10.1515/acve-2017-0031

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Citations (8)

West Nile virus infection in humans and other vertebrates

Archives of Biological Sciences (2014)

Ivana Hrnjaković-Cvjetković Vesna Milošević Vladimir Petrović Gordana Kovačević Jelena Radovanov

The West Nile virus is an arthropod borne or ARBO virus from the Flaviviridae family, which is maintained in nature in the transmission cycle between hosting birds and ornithophilic mosquito vectors. The virus is capable of infecting different vertebrate species and 60 mosquito species. The infection in humans can be asymptomatic or it can have different clinical manifestations ranging from light febrile diseases to fatal meningoencephalitis. This paper presents recent findings on the activity of the West Nile virus in Europe, the USA and Serbia. Presented are the results of serological testing of human populations and animals in Serbia, and the methods of molecular diagnostics to prove the existence of the virus.

West Nile virus

Flavivirus

10.2298/abs1401037h

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THE CONTROL OF CLASSICAL SWINE FEVER VIRUS PRESENCE IN WILD BOARS POPULATION

Biotechnology in Animal Husbandry (2009)

Jasna Prodanov-Radulović Radoslav Došen Ivan Pušić Tamaš Petrović D. Orlic̆

In our country a certain number of wild boars is controlled and reared in an enclosed hunting ground while the number of free-ranging population is mainly unknown. It is not easy to achieve and often not possible entirely to perform a complete diagnostic examination on the presence of classical swine fever virus (CSFV) in wild boars in each evaluated case. The aim of the research was control of CSFV presence in wild boars population in five enclosed hunting area in Vojvodina. As part of health control programm of wild boars, shot by hunters, the examination of the trunci deriving from 30 wild boars was performed. The laboratory testing of tissue samples deriving from 15 shot wild boars was done in regular hunting season. In order to check health status, the pathomorphological examination of 12 carcasses of dead or diseased animals shot by hunters and laboratory testing of their organs and tissue samples was performed. One of the characteristics of outdoor swine production in some regions is raising free-roaming domestic pigs, where they share forest habitat with the wild boars. In epidemiological research of the problem of free-roaming domestic pigs, the material included domestic pigs located in forest habitat. Applying laboratory testing (ELISA test) on the tissue samples deriving from wild boars the presence of antigen of CSFV was not detected, while in free-roaming domestic pigs clinical, patological and laboratory results were positive. The control of wild boars population on the CSFV is complex and requires applying the specific plan which takes into consideration the disease characteristics, wild boar ethology, interaction which occurs between wild boars and free-roaming domestic animals and interfering human activities.

Classical swine fever

Hunting season

Wild boar

Domestic animal

Domestic pig

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Possibility for use of RT-PCR technique in establishing presence of bovine viral diarrhea virus in sperm of breeding bulls

Veterinarski glasnik (2005)

Tamaš Petrović Sava Lazić Milovan Jovičin B. Djuričič

The bovine viral diarrhea (BVD) virus is a significant health-economic pathogen in cattle which can be excreted and spread also through sperm of persistently or acutely infected bulls. Native sperm of 6 bulls, found to be negative to the BVD virus by isolating the virus and using the RT-PCR method, was experimentally infected with a tenfold dilution of the non-cytopathogen 22146 strain of the BVD virus with a titer of 105,5. This way, dilutions of the BVD virus from 10-1 to 10-6 (5 x 104 TCID/50 do 0,5 TCID/50 in 0.1 ml native sperm were obtained. From sperm infected in this way, the virus was reisolated on FTB cell culture in a microtiter plate with 96 pools in which each sample of the infected sperm was set up in three samples, and each of them was titrated to a dilution of 1:2 to 1:256. The presence of the BVD virus was proven using the technique of fluorescent antibodies in a second blind passage on FTB culture cells. For cell culture an extremely toxic effect of native sperm to a dilution of 1:64 was established. The BVD virus was reisolated from sperm in all three sperm samples with 5 x 104, 5 x 103 i 5 x 102 TCID/50, and it was not reisolated from sperm with 50, with 5, and with 0.5 TCID/50 BVD virus in 0.1 ml native sperm. At the same time, the presence of the BVD viral genome was proved using the RT-PCR method in the same samples of artificially infected native sperm of bulls. A positive re suit was established in native sperm with 5 x 104, 5 x 103, 5 x 102 and 50 TCID/50 BVD virus n 0.1 ml native sperm. The experiment proved that the RT-PCR method has advantages over the isolation of the BVD virus from samples of native sperm of bulls. These are: shortterm investigations (1 to 2 days) and greater sensitivity (10 times bigger than the isolation of the virus). The isolation of the virus takes at least 10 days, and its greater sensitivity is primarily a result of the cyrotoxic effect of native sperm of bulls on cell culture.

Serial dilution

10.2298/vetgl0504371p

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Schoolchildren's family diet in yugoslav study of precursors of atherosclerosis

Atherosclerosis (1999)

Momčilo Pavlović Agneš Kadvan Tamaš Petrović M. Mirković Mira Pavkovic

10.1016/s0021-9150(99)80518-8

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