Mariita Rodriguez Orbegoso

Universität Hamburg

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Christel Schmeisser

Universität Hamburg

Wolfgang R. Streit

Universität Hamburg

Hari B. Krishnan

University of Missouri

Dagmar Krysciak

Universität Hamburg

Jörg Schuldes

University of Göttingen

Lei Li

Shenzhen Bay Laboratory

Christian Utpatel

Research Center Borstel - Leibniz Lung Center

Jessica Grote

Universität Hamburg

Rolf Daniel

University of Göttingen

Konrad U. Förstner

ZB MED - Information Centre for Life Sciences

Cooperative Institutions

Universität Hamburg

University of Göttingen

University of Duisburg-Essen

Heinrich Heine University Düsseldorf

University of Missouri

Agricultural Research Service

United States Department of Agriculture

Apollo Hospitals

Karpagam Academy of Higher Education

All India Institute of Medical Sciences Raipur

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RNA Sequencing Analysis of the Broad-Host-Range Strain Sinorhizobium fredii NGR234 Identifies a Large Set of Genes Linked to Quorum Sensing-Dependent Regulation in the Background of a traI and ngrI Deletion Mutant

Applied and Environmental Microbiology (2014)

Dagmar Krysciak Jessica Grote Mariita Rodriguez Orbegoso Christian Utpatel Konrad U. Förstner

ABSTRACT The alphaproteobacterium Sinorhizobium fredii NGR234 has an exceptionally wide host range, as it forms nitrogen-fixing nodules with more legumes than any other known microsymbiont. Within its 6.9-Mbp genome, it encodes two N -acyl-homoserine-lactone synthase genes (i.e., traI and ngrI ) involved in the biosynthesis of two distinct autoinducer I-type molecules. Here, we report on the construction of an NGR234-Δ traI and an NGR234-Δ ngrI mutant and their genome-wide transcriptome analysis. A high-resolution RNA sequencing (RNA-seq) analysis of early-stationary-phase cultures in the NGR234-Δ traI background suggested that up to 316 genes were differentially expressed in the NGR234-Δ traI mutant versus the parent strain. Similarly, in the background of NGR234-Δ ngrI 466 differentially regulated genes were identified. Accordingly, a common set of 186 genes was regulated by the TraI/R and NgrI/R regulon. Coregulated genes included 42 flagellar biosynthesis genes and 22 genes linked to exopolysaccharide (EPS) biosynthesis. Among the genes and open reading frames (ORFs) that were differentially regulated in NGR234-Δ traI were those linked to replication of the pNGR234 a symbiotic plasmid and cytochrome c oxidases. Biotin and pyrroloquinoline quinone biosynthesis genes were differentially expressed in the NGR234-Δ ngrI mutant as well as the entire cluster of 21 genes linked to assembly of the NGR234 type III secretion system (T3SS-II). Further, we also discovered that genes responsible for rhizopine catabolism in NGR234 were strongly repressed in the presence of high levels of N -acyl-homoserine-lactones. Together with nodulation assays, the RNA-seq-based findings suggested that quorum sensing (QS)-dependent gene regulation appears to be of higher relevance during nonsymbiotic growth rather than for life within root nodules.

Regulon

Homoserine

10.1128/aem.01835-14

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Citations (31)

Molecular Keys to Broad Host Range in Sinorhizobium fredii NGR234, USDA257, and HH103

Biological nitrogen fixation (2015)

Dagmar Krysciak Mariita Rodriguez Orbegoso Christel Schmeisser Wolfgang R. Streit

Sinorhizobium

10.1002/9781119053095.ch32

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Complete Genome Sequence of the Broad-Host-Range Strain Sinorhizobium fredii USDA257

Journal of Bacteriology (2012)

Jörg Schuldes Mariita Rodriguez Orbegoso Christel Schmeisser Hari B. Krishnan Rolf Daniel

Here we announce the complete genome sequence of the symbiotic and nitrogen-fixing bacterium Sinorhizobium fredii USDA257. The genome shares a high degree of sequence similarity with the closely related broad-host-range strains S. fredii NGR234 and HH103. Most strikingly, the USDA257 genome encodes a wealth of secretory systems.

Sinorhizobium meliloti

Strain (injury)

Sinorhizobium

Sequence (biology)

10.1128/jb.00966-12

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Citations (42)