José Reginaldo Nascimento Brito

Universidade Federal do Pará

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Edivaldo Herculano Corrêa de Oliveira

Universidade Federal do Pará

Wallax Augusto Silva Ferreira

AC Camargo Hospital

Nilson Praia Anselmo

Universidade Federal do Pará

Fernanda do Espírito Santo Sagica

Instituto Evandro Chagas

José Antônio Santos de Lima

Loyola Medicine

Igor Andrade Pessôa

Universidade Federal do Pará

Carolina Koury Nassar Amorim

Instituto Evandro Chagas

Bárbara do Nascimento Borges

Universidade Federal do Pará

Eric Homero Albuquerque Paschoal

Amazon Research Foundation

Geraldo Nascimento

Universidade de Pernambuco

Cooperative Institutions

Universidade Federal do Pará

Universidade de São Paulo

Instituto Evandro Chagas

University of Coimbra

Instituto Sócioambiental

Universidade Federal Rural da Amazônia

Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo

Jena University Hospital

Universidade Federal Rural de Pernambuco

Universidade Federal de Pernambuco

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Expression Analysis of Genes Involved in the RB/E2F Pathway in Astrocytic Tumors

PLoS ONE (2015)

Wallax Augusto Silva Ferreira Mariana Diniz Araújo Nilson Praia Anselmo Edivaldo Herculano Corrêa de Oliveira José Reginaldo Nascimento Brito

Astrocytic gliomas, which are derived from glial cells, are considered the most common primary neoplasias of the central nervous system (CNS) and are histologically classified as low grade (I and II) or high grade (III and IV). Recent studies have shown that astrocytoma formation is the result of the deregulation of several pathways, including the RB/E2F pathway, which is commonly deregulated in various human cancers via genetic or epigenetic mechanisms. On the basis of the assumption that the study of the mechanisms controlling the INK4/ARF locus can help elucidate the molecular pathogenesis of astrocytic tumors, identify diagnostic and prognostic markers, and help select appropriate clinical treatments, the present study aimed to evaluate and compare methylation patterns using bisulfite sequencing PCR and evaluate the gene expression profile using real-time PCR in the genes CDKN2A, CDKN2B, CDC6, Bmi-1, CCND1, and RB1 in astrocytic tumors. Our results indicate that all the evaluated genes are not methylated independent of the tumor grade. However, the real-time PCR results indicate that these genes undergo progressive deregulation as a function of the tumor grade. In addition, the genes CDKN2A, CDKN2B, and RB1 were underexpressed, whereas CDC6, Bmi-1, and CCND1 were overexpressed; the increase in gene expression was significantly associated with decreased patient survival. Therefore, we propose that the evaluation of the expression levels of the genes involved in the RB/E2F pathway can be used in the monitoring of patients with astrocytomas in clinical practice and for the prognostic indication of disease progression.

CDKN2B

p14arf

Bisulfite sequencing

10.1371/journal.pone.0137259

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Citations (16)

Meningiomas: An Overview of the Landscape of Copy Number Alterations in Samples from an Admixed Population

Journal of Oncology (2020)

Michele Amaral da Silveira Wallax Augusto Silva Ferreira Carolina Koury Nassar Amorim José Reginaldo Nascimento Brito André Salim Kayath

Meningiomas are considered the most common intracranial tumors, affecting mainly women. Studies in mixed populations can be of great importance to clarify issues related to the genetic diversity of tumors and their development. Considering that data obtained from analyses of the profile of copy number alterations (CNA) have been a useful diagnostic indicator for many types of tumors and that meningiomas show a complex pattern of gains and losses in the number of copies, our objective was to analyze the CNA profile in 33 samples of meningiomas of different histological grades (WHO Grade I-III) from patients in a city located in the Amazon region of Brazil, using aCGH. We found that the female to male ratio was 3 : 1. The aCGH analysis revealed a total of 2304 CNA, with an average of 69.8 ± 57.4 per case, of which 1197 were gains (52%), 926 were losses (40.2%), 105 were amplifications (4. 5%), and 76 were deletions (3.3%). A significant relationship was observed between the type of CNA and the degree of the tumor (chi-square test: χ 2 = 65,844; p<0.0001; contingency coefficient: C = 0.1772; p<0.0001). Evaluating the recurrent changes in at least 50% of the samples, we observe as the most frequent losses of the segments 22q13.1-q13.2 (82%), 1p35.3 (76%), and 14q13.1-q13.2 (67%), involving all histopathological grades. The analysis of these regions showed the inclusion of genes with functions such as regulation, maintenance of cell survival, reorganization of the cytoskeleton, cell signaling, and DNA repair, among others. However, overall, the profiles observed in meningiomas of this admixed population were very similar to the ones observed in Caucasian groups. An interesting finding was a recurrent gain of 8p22 observed only in grade I meningiomas, a region which includes DLC1, a suppressor candidate gene probably implicated in the developments or progression of meningiomas, usually found deleted, when related to CNAs.

Contingency table

Korean population

10.1155/2020/3821695

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Detection and Correlation of Single and Concomitant TP53, PTEN, and CDKN2A Alterations in Gliomas

International Journal of Molecular Sciences (2019)

Igor Andrade Pessôa Carolina Koury Nassar Amorim Wallax Augusto Silva Ferreira Fernanda do Espírito Santo Sagica José Reginaldo Nascimento Brito

Gliomas are the most frequent primary tumors of central nervous system and represent a heterogeneous group of tumors that originates from the glial cells. TP53, PTEN, and CDKN2A are important tumor suppressor genes that encode proteins involved in sustaining cellular homeostasis by different signaling pathways. Though genetic alterations in these genes play a significant role in tumorigenesis, few studies are available regarding the incidence and relation of concomitant TP53, PTEN, and CDKN2A alterations in gliomas. The purpose of this study was to evaluate the occurrence of mutation and deletion in these genes, through single-strand conformational polymorphism, array-comparative genomic hybridization, and fluorescence in situ hybridization techniques, in 69 gliomas samples. Molecular results demonstrated a significant higher prevalence of TP53, PTEN, and CDKN2A alterations in astrocytoma than other tumor subtypes, and heterozygous deletion was the most frequent event. In addition, a significant association was observed between TP53 and CDKN2A alterations (p = 0.0424), which tend to coexist in low grade astrocytomas (5/46 cases (10.9%)), suggesting that they are early events in development of these tumors, and PTEN and CDKN2A deletions (p = 0.0022), which occurred concomitantly in 9/50 (18%) patients, with CDKN2A changes preceding PTEN deletions, present preferably in high-grade gliomas.

Haploinsufficiency

10.3390/ijms20112658

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Citations (27)

Brainstem abscess treated conservatively

Surgical Neurology International (2020)

Camila Furtado Leão Maira Piani Couto José Antônio Santos de Lima Eric Homero Albuquerque Paschoal José Reginaldo Nascimento Brito

Brainstem abscess is a rare condition with a variety of treatment approaches. In this paper, we report an unusual case of a brainstem abscess with a positive outcome in an immunocompetent patient who was treated with antibiotic therapy.A 22-year-old female presented with bilateral tetraparesis that was worse on the left hemibody, appendicular tremor, and left upper eyelid ptosis. Brain magnetic resonance imaging showed an abscess in the pons and midbrain due to possible nocardiosis. She was treated with dexamethasone, phenytoin, vancomycin, and meropenem for 8 weeks and trimethoprim-sulfamethoxazole for 6 weeks. The brain injury decreased, and the patient's neurological status significantly improved.Brainstem abscess may be treated conservatively, leading to improvement of the clinical condition and decreased lesion size on imaging.

Brain abscess

Pons

10.25259/sni_569_2019

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Citations (3)

MTHFR C677T polymorphism not associated with meningiomas: Study of an interethnic Brazilian population, and meta-analysis

Meta Gene (2020)

Carlos Eduardo Matos Carvalho Bastos Bárbara do Nascimento Borges Isabela Guerreiro Diniz José Reginaldo Nascimento Brito Nilson Praia Anselmo

10.1016/j.mgene.2020.100795

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Citations (1)

Iconography : Hemobilia due to pseudoaneurysm of the cystic artery

Helena Tavares de Sousa Pedro Amaro José Reginaldo Nascimento Brito JESSYCA MICHELLE ALMEIDA M R Silva

Pseudoaneurysm

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Dual origin of vertebral artery and intimal relationship with spontaneous artery dissection: a case report and systematic review

Surgical and Radiologic Anatomy (2021)

Derik Williams Barros do Nascimento Rafaella Macêdo Monteiro da Cruz Gabriel Gomes Diniz Pimenta Fernando Mendes Paschoal Antônio de Mattos Lima Neto

Pseudoaneurysm

10.1007/s00276-020-02638-w

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Stereotactic radiosurgery for the treatment of Glomus Jugulare Tumors

Surgical Neurology International (2013)

Emerson Magno de Andrade José Reginaldo Nascimento Brito SusanaDias Mario Suely Maymone de Melo Salomon Benabou

The glomus jugulare tumor is a slowly growing benign neoplasm originating from neural crest. There is a high morbidity associated with surgical resection of glomus jugulare. Radiosurgery play a relevant role as a therapeutic option in these tumors and its use has grown in popularity. The authors describe a retrospective series of 15 patients and reviewed the literature about the glomus jugulare tumors.We reviewed retrospectively the data of 15 patients treated with stereotactic linear accelerator stereotactic radiosurgery (LINAC) radiosurgery between 2006 and 2011.The average tumor volume was 18.5 cm(3). The radiation dose to the tumor margin ranged between 12 and 20 Gy. The neurological status improved in three patients and remained unchanged in 12 patients. One patient developed a transient 7(th) nerve palsy that improved after clinical treatment. All tumors remained stable in size on follow-up with resonance magnetic images.The radiosurgery is a safe and effective therapy for patients with glomus jugulare tumor. Despite the short follow-up period and the limited number of patients analyzed, we can infer that radiosurgery produce a tumor growth control with low morbidity, and may be used as a good option to surgical resection in selected cases.

Glomus tumor

10.4103/2152-7806.121629

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Citations (25)

Is Ethnobiologist Training at Risk? Challenges of a Brazilian Graduate Program

Ethnobiology and Conservation (2025)

Valdir de Moura Brito Júnior Ana Alves Ana Maria Siqueira Quirino Aníbal Silva Cantalice Antônio Celso Magalhães

The Graduate Program in Ethnobiology and Nature Conservation (PPGEtno, an acronym in Portuguese) was established in 2011 at the Federal Rural University of Pernambuco (UFRPE, an acronym in Portuguese) in collaboration with other institutions in Northeast Brazil. PPGEtno addressed the urgent need to train professionals sensitive to biodiversity conservation issues through an interdisciplinary approach that values traditional knowledge and the relationship between people and nature. Despite its success, PPGEtno faces administrative challenges due to regulatory constraints, which restrict coordination roles only UFRPE faculty members to hold, which is currently limited to just five faculty members from the UFRPE, the program’s host institution. The lack of faculty members aligned with social-ecological thinking at UFRPE exacerbates the situation, putting the program at risk of extinction or reduction to a research line within another graduate program. PPGEtno's academic output is significant, with 96 graduates, 1,473 published articles, and important contributions to Ethnobiology and Ecology. Its publications include high-impact journals and cover a wide range of topics. The program's closure would represent an immeasurable loss for research on biodiversity and human-nature interactions. In this manuscript, we demonstrate the impacts that the absence of PPGEtno could cause, both theoretically and practically. The continuation of PPGEtno is vital for advancing Ethnobiology as a scientific discipline, serving a critical role in training ethnobiologists and fostering new knowledge on biodiversity conservation and human-nature relationships in Brazil and across Latin America.

10.15451/ec2025-02-14.13-1-9

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IDH1 and IDH2 mutations in different histologic subtypes and WHO grading gliomas in a sample from Northern Brazil

Genetics and Molecular Research (2015)

Igor Andrade Pessôa Fernanda do Espírito Santo Sagica Nilson Praia Anselmo José Reginaldo Nascimento Brito Edivaldo Herculano Corrêa de Oliveira

Glioma is a term used to describe tumors derived from glial cells.These tumors are divided into subgroups based on the histological morphology and similarity of their differentiated glia cells.Traditionally, they are classified according to the World Health Organization and include astrocytomas, oligodendrogliomas, ependymomas, and oligoastrocytomas.Like most cancers, gliomas develop as a result of genetic changes that accumulate with tumor progression.Alterations in isocitrate dehydrogenase 1 (IDH1) and IDH2 were found to be relevant in the classification and prognostic of gliomas.Because of the importance of mutations in these genes, particularly in ©FUNPEC-RP www.funpecrp.com.brGenetics and Molecular Research 14 (2): 6533-6542 (2015)IDH1, in different proposals of the genesis and progression of gliomas, we analyzed the occurrence of mutations in these genes in samples obtained from patients from Belém (PA, Brazil) using polymerase chain reaction-single-strand conformation polymorphism followed by sequencing.We compared the results obtained from tumors of different malignancy grades, evaluating the significance of the associations between different variables.R132H was the only mutation found in 17.6% (6/34) of cases, including in astrocytomas, anaplastic astrocytomas, oligodendroglioma, and anaplastic oligoastrocytoma.No mutations were found in the IDH2 gene.We found no significant relationship between the identified mutations in IDH1 and the variables.Our data could not confirm that mutations in IDH1/IDH2 are indicative of malignancy and prognosis.However, the results support that the mutation in IDH1 gene was an early event in the development of gliomas, as it was found in tumors of different malignancy grades.

IDH2

Isocitrate dehydrogenase

Anaplastic astrocytoma

Grading (engineering)

Oligodendroglial Tumor

10.4238/2015.june.12.6

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Citations (5)