Viktor Liashko

Ministry of Health

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В. И. Кашуба

Karolinska Institutet

Nataliia Melnichuk

Institute of Molecular Biology and Genetics

Antti Hakanen

University of Turku

Endre Ludwig

Semmelweis University

Paul Naaber

University of Tartu

Kristján Orri Helgason

National University Hospital of Iceland

Sören Gatermann

Ruhr University Bochum

L. M. Chernenko

Ministry of Health

Akif Gurbanov

M. A. Tukalo

Institute of Molecular Biology and Genetics

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Institute of Molecular Biology and Genetics

National Academy of Sciences of Ukraine

European Society of Clinical Microbiology and Infectious Diseases

Centre Hospitalier Régional et Universitaire de Nancy

Hospital for Tropical Diseases

CNR de la Résistance aux Antibiotiques

Institute of Medical Microbiology and Hygiene

Sorbonne Université

Inserm

Centre d'Immunologie et des Maladies Infectieuses

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Gene expression profile in peripheral blood cells of patients with COVID-19

Proceedings of 7th International Electronic Conference on Medicinal Chemistry (2020)

Nataliia Melnichuk Viktor Liashko В. И. Кашуба Zenoviy Tkachuk

The clinical course of coronavirus disease 2019 (COVID‐19) varies from mild symptoms to acute respiratory distress syndrome, hyperinflammation, and coagulation disorder. The hematopoietic system plays a critical role in the observed hyperinflammation, particularly in severely ill patients. Using peripheral blood cells (PBCs) for gene expression analysis is valuable to evaluate disease-associated and drug-response related genes. In this study, we aimed to explore the gene expression profile of PBCs in patients with COVID‐19. Material & methods. Whole blood samples were collected from 19 patients with acute COVID‐19 infection and 20 healthy volunteers. The gene expression of PBCs was determined by RT-qPCR. Results. We investigated the expression of cytokines, chemokines, interferon-stimulated pro-oxidation, and coagulation genes in PBCs of the infected and healthy samples. Up-regulated expression of some genes was found out in the blood of COVID‐19-infected patients compared to the healthy sample. Conclusions: We have identified some genes in whole blood that classifies COVID‐19-infected and healthy patients with good accuracy. These results suggested that the expression of cytokines, coagulation, and interferon-stimulated genes in PBCs can be used for early detection of hyperinflammation, coagulation disorders, and evaluation of efficiency treatment of this disease.

10.3390/ecmc2020-07298

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Activities performed by the State Sanitary and Epidemiological Service of Ukraine in control over sanitary and epidemiological situation during EURO-2012.

A. Kravchuk Viktor Liashko

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Identification and characterization of the SARS-CoV-2 lineage B.1.1.7 upon the new outbreak of the COVID-19 in Ukraine in February 2021

Biopolymers and Cell (2021)

В. И. Кашуба N. V. Hryshchenko G. V. Gerashchenko Nataliia Melnichuk Tetiana Marchishak

Aim.To identify and characterize the SARS-CoV-2 variants, collected upon the new wave of COVID-19 outbreak in Ivano-Frankivs'k region of Ukraine, using the whole genome genotyping.Methods.The parallel whole genome sequencing was performed on the processed RNA, isolated from nasopharyngeal swabs of 19 patients, using an Ion GeneStudio S5 Plus System.Results.All the identified SARS-CoV-2 genotypes were referred to 20I/501Y.V1 clade, the variant VUI202012/01 GRY (the B.1.1.7 lineage).In the analyzed virus variants forty-seven various mutations were found.Besides the founder 20I/501Y.V1 missense mutations, several unique alterations were detected, including those in the S-and N-proteins of SARS-CoV-2, that might have clinical and epidemiological relevance.Conclusions.The current wave of the COVID-19 outbreak in Ukraine is associated not only with seasonal fluctuations in the virus transmission, but also with the emergence of more aggressive virulent variants, such as B.1.1.7,which basically displaced previous strains and affects the younger population.

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2019-20 coronavirus outbreak

10.7124/bc.000a52

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Cholera outbreak in Donetsk Oblast, Ukraine, in 2011.

Viktor Liashko

Cholera

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