Chenghai Peng

South China Agricultural University

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Jiali Cheng

Huazhong University of Science and Technology

Maoqing Wang

Harbin Medical University

Ye Tian

Harbin Medical University

Fan Wang

Harbin Medical University

Zhen Tian

Heilongjiang Institute of Technology

Wenwu Cao

University of Science and Technology Beijing

Sa Shi

Harbin Medical University

Zhiping Long

Harbin Medical University

Zhiguo Zhang

Harbin Institute of Technology

Yongzhi Sun

Johns Hopkins University

Cooperative Institutions

Harbin Medical University

First Affiliated Hospital of Harbin Medical University

Chinese Academy of Sciences

Chinese Academy of Medical Sciences & Peking Union Medical College

Second Affiliated Hospital of Harbin Medical University

Zhejiang University

Nanchang University

Huazhong University of Science and Technology

Harbin Institute of Technology

Ministry of Education of the People's Republic of China

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Apoptosis of vascular smooth muscle cells induced by photodynamic therapy with protoporphyrin IX

Biochemical and Biophysical Research Communications (2009)

Qingsong Li Jiali Cheng Chenghai Peng Zhitao Li Sa Shi

Protoporphyrin IX

Viability assay

10.1016/j.bbrc.2009.11.003

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Citations (11)

Marginal zinc deficiency alters the heart proteome of rats

Food & Function (2023)

Yongzhi Sun Jianfeng Chen Hongmei Wang Xinyu Song Zhaohui Sun

Zinc deficiency is closely related to cardiovascular diseases (CVDs), but the effects of marginal zinc deficiency (MZD) after birth on the heart are unknown. In this study, 4-week-old male rats were fed a low zinc diet (10 mg kg-1, 1/3 recommended nutrient intake, RNI) for 8 weeks. Echocardiography and histopathology were performed to assess the functional and morphological alterations of the heart. High-throughput proteomics was used to study the effects of MZD on cardiac protein expression. We found that MZD reduced food intake, body weight, serum zinc, and heart weight; however, the coefficient, zinc concentration, function, and histopathology of the heart were not changed. The heart proteome was altered in the marginal zinc-deficient diet group (MZG), compared with the normal zinc diet group (NZG). A total of 310 differentially expressed proteins (P < 0.05) were significantly changed by MZD, among which 163 proteins were up-regulated and 147 were down-regulated. Of these, 43 proteins are related to CVDs and 18 proteins are zinc-associated proteins. Gene Ontology and Pathway analysis revealed that 74 biological processes (BPs) and 37 pathways were significantly changed by MZD. This included six CVD-related BPs, such as regulation of heart rate, cardiac muscle contraction, regulation of ventricular cardiac muscle cell action potential, and regulation of blood pressure, and eight CVD-related pathways, such as dilated cardiomyopathy, diabetic cardiomyopathy, and hypertrophic cardiomyopathy. Our data show that marginal zinc deficiency after birth significantly alters cardiac protein expression and pathways related to CVDs.

Histopathology

Dilated Cardiomyopathy

Proteome

10.1039/d2fo03815c

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Citations (4)

High-throughput metabolomics for discovering potential biomarkers and identifying metabolic mechanisms in aging and Alzheimer’s disease

Figshare (2021)

Kun Xie Qi Qin Zhiping Long Yihui Yang Chenghai Peng

To analyse the plasma metabolites related to aging, we conducted an untargeted metabolomics analysis using ultra-high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry in a two-stage cross-sectional study. In total, 669 metabolites were significantly altered with aging.

10.6084/m9.figshare.13621664

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Hyperbaric Oxygen Preconditioning Attenuates Myocardium Ischemia-Reperfusion Injury Through Upregulation of Heme Oxygenase 1 Expression

Journal of Cardiovascular Pharmacology and Therapeutics (2015)

Xuesong Yin Xiaofeng Wang Zhixin Fan Chenghai Peng Zhongqiao Ren

Background: With the rise of the burden of ischemic heart disease, both clinical and economic evidence show a desperate need to protect the heart against myocardium ischemia-reperfusion injury-related complications following cardiac surgery or percutaneous coronary intervention. However, there is no effective intervention for myocardium ischemia-reperfusion injury as yet. Methods: We pretreated mice with 4 daily 2.0 absolute atmosphere (ATA) hyperbaric oxygen, then observed its effects on heart function parameters and infarct size following in situ ischemia-reperfusion. Multiple oxidative and inflammation products were measured in the myocardium. Next, we investigated the expression of heme oxygenase 1 (HO-1), phosphatidylinositol 3-kinase (PI3K)/serine/threonine protein kinase (Akt) pathway, and NF-E2-related factor 2 (Nrf2) in the presence of myocardium ischemia-reperfusion injury, hyperbaric oxygen preconditioning, and their inhibitors and their effects on heart function parameters. Results: Hyperbaric oxygen preconditioning ameliorated the cardiac function and histological alterations induced by myocardium ischemia-reperfusion injury, decreased oxidative products and proinflammatory cytokine. Hyperbaric oxygen preconditioning increased expression of HO-1, which was suppressed by PI3K inhibitor LY294002, Nrf2 knockout, and Akt inhibitor triciribine. The expression of Nrf2 was enhanced by hyperbaric oxygen preconditioning, but decreased by LY294002 and triciribine. The Akt was also activated by hyperbaric oxygen preconditioning but suppressed by LY294002. The hemodynamic assays showed that cardiac function was suppressed by LY294002, Nrf2 knockout, and triciribine. Conclusion: These data present a novel signaling mechanism by which hyperbaric oxygen preconditioning protects myocardium ischemia-reperfusion injury via PI3K/Akt/Nrf2-dependent antioxidant defensive system.

LY294002

Ischemic Preconditioning

Proinflammatory cytokine

10.1177/1074248414568196

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Citations (41)

High-Throughput Metabolomics for Discovering Potential Biomarkers and Identifying Metabolic Mechanisms in Aging and Alzheimer’s Disease

Research Square (Research Square) (2020)

Kun Xie Qi Qin Zhiping Long Yihui Yang Chenghai Peng

Abstract Background and Aims: Alzheimer’s disease (AD) is an aging-related neurodegenerative disease. The current diagnosis of AD may fail to identify a substantial number of asymptomatic individuals who will progress to AD. We aimed to investigate the metabolic mechanisms of aging and AD and to identify potential biomarkers for the early screening of AD in a natural aging population. Methods: To analyse the plasma metabolites related to aging, we conducted an untargeted metabolomics analysis using ultra-high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry in a two-stage cross-sectional study. Spearman’s correlation analysis and random forest were applied to model the relationship between age and each metabolite. Moreover, systematic reviews of metabolomics studies of AD in the PubMed, Cochrane and Embase databases were searched to extract the differential metabolites and altered pathways from original studies. Pathway enrichment analysis was conducted using Mummichog. Results: In total, 669 metabolites were significantly altered with aging, and thirteen pathways were enriched and correlated with aging. Five metabolites (palmitic acid, stearic acid, linoleic acid, glutamine, and oleic acid) were identified as potential biomarkers for AD based on a systematic review. Arginine and histidine were considered candidate monitoring markers of disease progression in the mild cognitive impairment (MCI) population. Moreover, three pathways (purine metabolism, arginine and proline metabolism, and the TCA cycle) were shared between aging and AD. Arginine and proline metabolism play a key role in the progression from CN to MCI and to AD in the natural aging population. Three metabolites, 16-a-hydroxypregnenolone, stearic acid and PC (16:0/22:5(4Z,7Z,10Z,13Z,16Z)), were finally proposed as potential markers of AD in the natural aging population. Conclusion: The underlying mechanism shared between aging and AD and the potential biomarkers for AD diagnosis were proposed based on multistep comparative analysis.

Metabolic pathway

10.21203/rs.3.rs-60073/v1

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Citations (2)

Rapid inhibition of atherosclerotic plaque progression by sonodynamic therapy

Cardiovascular Research (2018)

Xin Sun Shuyuan Guo Jianting Yao Huan Wang Chenghai Peng

Currently, efficient regimens to reverse atherosclerotic plaques are not available in the clinic. Herein, we present sonodynamic therapy (SDT) as a novel methodology to rapidly inhibit progression of atherosclerotic plaques.

Sonodynamic therapy

10.1093/cvr/cvy139

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Citations (51)

Correction: Marginal zinc deficiency alters the heart proteome of rats

Food & Function (2023)

Yongzhi Sun Jianfeng Chen Hongmei Wang Xinyu Song Zhaohui Sun

Correction for ‘Marginal zinc deficiency alters the heart proteome of rats’ by Yongzhi Sun et al. , Food Funct. , 2023, https://doi.org/10.1039/d2fo03815c.

Proteome

10.1039/d3fo90034g

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Citations (2)

Two Novel Angiotensin-Converting Enzyme (ACE) Inhibitory and ACE2 Upregulating Peptides from the Hydrolysate of Pumpkin (Cucurbita moschata) Seed Meal

Journal of Agricultural and Food Chemistry (2024)

Xin Li Chenghai Peng Suyao Xiao Qun Wang Aimei Zhou

Pumpkin (Cucurbita moschata) seed meal (PSM), the major byproduct of pumpkin seed oil industry, was used to prepare angiotensin-converting enzyme (ACE) inhibitory and angiotensin-converting enzyme 2 (ACE2) upregulating peptides. These peptides were isolated and purified from the PSM hydrolysate prepared using Neutrase 5.0 BG by ultrafiltration, Sephadex G-15 column chromatography, and reversed-phase high-performance liquid chromatography. Two peptides with significant ACE inhibition activity were identified as SNHANQLDFHP and PVQVLASAYR with IC50 values of 172.07 and 90.69 μM, respectively. The C-terminal tripeptides of the two peptides contained Pro, Phe, and Tyr, respectively, and PVQVLASAYR also had Val in its N-terminal tripeptide, which was a favorable structure for ACE inhibition. Molecular docking results declared that the two peptides could interact with ACE through hydrogen bonds and hydrophobic interactions. Furthermore, the two peptides performed protective function on EA.hy926 cells by decreasing the secretion of endothelin-1, increasing the release of nitric oxide, and regulating the ACE2 activity. In vitro simulated gastrointestinal digestion showed the two peptides exhibited good stability against gastrointestinal enzyme digestion. In conclusion, PSM is a promising material for preparing antihypertensive peptides.

Cucurbita moschata

Pumpkin seed

Tripeptide

Sephadex

IC50

10.1021/acs.jafc.4c00609

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Citations (5)

An animal model of atherosclerotic plaque disruption and thrombosis in rabbit using pharmacological triggering to plaques induced by perivascular collar placement

Cardiovascular Pathology (2013)

Xin Sun Wei Cao Jinjin Cui Liping Wang Lansi Ma

Collar

Rabbit (cipher)

Animal model

10.1016/j.carpath.2012.11.003

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Citations (6)

HEMATOPORPHYRIN MONOMETHYL ETHER-MEDIATED PHOTODYNAMIC EFFECTS ON MACROPHAGES

Heart (2012)

Jiali Cheng Qingsong Li Chenghai Peng Sa Shi Ye Tian

Objectives

Photodynamic therapy (PDT) has been shown to attenuate atherosclerotic plaque progression and decrease macrophage-infiltration. The effectiveness of PDT depends strongly on the type of photosensitizers. Hematoporphyrin monomethyl ether (HMME) is a promising second-generation porphyrin-related photosensitizer for PDT. This study is designed to investigate HMME mediated Photodynamic therapy on macrophage and define the cell-death pathway.

Methods

Fluorescence of HMME with different incubated time was tested. Use confocal scanning laser microscope to investigate HMME subcellular distribution. THP-1 derived macrophages were cultured with HMME for 2 h and then exposed to 635 nm diode lasers for 1.5 min and 3 min. Six-hours later, cell viability analysis was performed with 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay and annexin V/PI staining by flow cytometer. The activities of caspase-9 and caspase-3 were measured using the fluorescent assay kits.

Results

Our data demonstrated that the intensity of laser-induced HMME fluorescence in macrophages steadily increased with the increasing incubation concentration of HMME. The survival rate of macrophages determined by MTT assay decreased with the increasing HMME concentration and irradiation time. HMME-based PDT induced macrophage apoptosis via caspase-9 and caspase-3 activation pathway detected by caspase fluorescent assay kit and flow cytometer. The 1.5 min group increased the number of apoptotic macrophages 5.36±3.24 at 12 h post irradiation (mean±SD, n=3, p<0.05), whereas the 3 min group increased the number of apoptotic macrophages 67.18±5.17 (mean±SD, n=3, p<0.001).

Conclusions

Our data demonstrated that HMME could accumulate in macrophages and HMME-mediated PDT induced macrophage apoptosis. These results imply that photodynamic therapy with HMME may therefore be a useful clinical treatment for unstable atherosclerotic plaques.

MTT assay

Hematoporphyrin

10.1136/heartjnl-2012-302920a.137

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Citations (1)