Cristina da Silva Meira

Instituto Adolfo Lutz

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Vera Lúcia Pereira‐Chioccola

Instituto Adolfo Lutz

Luíz Carlos de Mattos

Faculdade de Medicina de São José do Rio Preto

Cinara Cássia Brandão de Mattos

Faculdade de Medicina de São José do Rio Preto

Fábio Batista Frederico

Hospital de Base

J Vidal

Instituto de Infectologia Emílio Ribas

Ricardo Gava

Instituto Adolfo Lutz

Thais A. Costa‐Silva

Universidade Federal do ABC

Gabriela Motoie

Instituto Adolfo Lutz

Marta Marques Maia

Instituto Adolfo Lutz

Rubens Camargo Siqueira

Faculdade de Medicina de São José do Rio Preto

Cooperative Institutions

Faculdade de Medicina de São José do Rio Preto

Instituto Adolfo Lutz

Universidade de São Paulo

Instituto de Infectologia Emílio Ribas

Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo

Hospital de Base

Universidade Estadual Paulista (Unesp)

Instituto de Medicina Tropical

Universidade Federal de São Paulo

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Performance of cryptococcal antigen lateral flow assay in serum, cerebrospinal fluid, whole blood, and urine in HIV-infected patients with culture-proven cryptococcal meningitis admitted at a Brazilian referral center

Revista do Instituto de Medicina Tropical de São Paulo (2018)

J Vidal C Zenatti Adriana Paulino Arnaldo Lopes Colombo Marilena dos Anjos Martins

Cryptococcal meningitis is the most common cause of opportunistic meningitis in HIV-infected patients in Brazil and causes unacceptable high mortality rates. In this study, HIV-infected patients with a first episode of culture-proven cryptococcal meningitis in cerebrospinal fluid (CSF) were prospectively included in order to evaluate sensitivity of cryptococcal antigen (CrAg) lateral flow assay (LFA) in serum, CSF, whole blood (fingerstick), and fresh urine. In addition, HIV-infected patients with other neurological confirmed diseases were included in order to evaluate the specificity of CrAg LFA in serum. Twenty patients with cryptococcal meningitis were included and in 19 of them, CrAg LFA in CSF, serum, and whole blood were positive (95% sensitivity). In 18 patients, India ink test was positive in CSF (90% sensitivity), and in 16 cases, CrAg LFA was positive in urine (80% sensitivity). Thirty-six HIV-infected patients with other neurological diseases had negative results of CrAg LFA in serum (100% specificity). In conclusion, CrAg LFA in serum, CSF, and whole blood showed high sensitivity and specificity. Whole blood CrAg LFA seems to be a good and reliable strategy to improve AIDS-related cryptococcal meningitis diagnosis in Brazil.

Blood Culture

10.1590/s1678-9946201860001

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Citations (20)

Toxoplasmose ocular com reação em cadeia da polimerase positiva em sangue periférico – relato de dois casos, estado de São Paulo, Brasil

Scientia Medica (2016)

Amanda Pires Barbosa Fábio Batista Frederico Mariana Previato Ana Iara da Costa Ferreira Plínio Pereira Martins Neto

Aims: To describe the use of polymerase chain reaction (PCR) in peripheral blood and demonstrate its importance in the clinical follow-up of patients with ocular toxoplasmosis.Case description: Two immunocompetent patients were clinically diagnosed with acute ocular toxoplasmosis. The routine clinical evaluation consisted of fundus examination using binocular indirect ophthalmoscopy, color fundus photography, fluorescein angiography, and spectral domain optical coherence tomography. The serological diagnosis was made by ELISA (IgM, IgG) and confirmed by ELFA (IgG, IgM). The molecular diagnosis was made by PCR in peripheral blood using the B1 gene of Toxoplasma gondii as marker. The younger patient was male, had previous lesion in the right eye, complained of low visual acuity in the left eye and was under treatment. The older patient was male, had retinal detachment, and presented with sudden loss of acuity in the right eye. The fundus examination revealed chorioretinal scar in the left eye. IgG was reactive, IgM was non-reactive, and PCR was positive in the peripheral blood of both patients. New blood samples were collected for serological and molecular monitoring and PCR remained positive in both cases. Six weeks after treatment with oral sulfadiazine and pyrimethamine, the PCR yielded negative results.Conclusions: The results show that T. gondii antigens may be found in peripheral blood during ocular reactivations and that PCR may be a good tool for the follow-up of patients with ocular toxoplasmosis.

Toxoplasmosis

Fundus (uterus)

Fundus Photography

10.15448/1980-6108.2015.4.20932

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Reference genes for studies in infectious parasitic diseases in five types of human tissues

Gene Reports (2017)

Cristina da Silva Meira Vera Lúcia Pereira‐Chioccola Marta Marques Maia Daise Damaris Carnietto de Hippólito Hui Tzu Lin‐Wang

Reference Genes

Glyceraldehyde 3-phosphate dehydrogenase

Housekeeping gene

10.1016/j.genrep.2017.03.002

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Citations (5)

miRNA 511_5p is a potential biomarker for ocular toxoplasmosis

Transactions of the Royal Society of Tropical Medicine and Hygiene (2023)

Geraldo Magela de Faria Laurie Sayuri Kumano Isabela Bronchtein Gomes Gláucio Silva Camargos Cristina da Silva Meira

ABSTRACT Background Ocular toxoplasmosis (OT) is a frequent clinical manifestation due to infection by Toxoplasma gondii. It is characterized by an inflammatory process involving macrophages activated by pro-inflammatory cytokines. The expression of microRNAs takes place during the inflammatory process and, among them, miRNA 511 regulates the activation of macrophages. This study evaluated the expression of miRNA 511_5p in patients with OT and healthy controls. Methods A total of 361 patients from the Hospital de Base of Fundação Faculdade de Medicina de São José do Rio Preto were enrolled and divided into four groups: G1–patients with active ocular lesions and reagent serology for T. gondii; G2–patients with scars and reagent serology for T. gondii; G3–patients without ocular lesions or scars and reagent serology for T. gondii; G4–patients without ocular lesions or scars and non-reagent serology for T. gondii. All patients underwent clinical and laboratory evaluation to confirm the diagnosis of OT. Serology tests, RNA extraction and cDNA synthesis were performed. Results The miRNA 511_5p levels were compared among the groups. The G1 group showed a high blood plasma concentration of miRNA 511_5p (mean 22.34) compared with the G2 (4.65), G3 (8.91) and G4 (3.52) groups (p<0.0001). Conclusion These data suggest that miRNA 511_5p has significant potential as a biomarker for OT.

Toxoplasmosis

10.1093/trstmh/trad045

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Citations (3)

Evaluation of serological and molecular tests used to identify Toxoplasma gondii infection in pregnant women attended in a public health service in São Paulo state, Brazil

Diagnostic Microbiology and Infectious Disease (2017)

F. Murata Marina Neves Ferreira Vera Lúcia Pereira‐Chioccola Lígia Cosentino Junqueira Franco Spegiorin Cristina da Silva Meira

Concordance

Toxoplasmosis

Kappa

10.1016/j.diagmicrobio.2017.06.004

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Citations (7)

Molecular detection of Leishmania (Leishmania) infantum in phlebotomine sandflies from a visceral leishmaniasis endemic area in northwestern of São Paulo State, Brazil

Acta Tropica (2018)

Kate Bastos dos Santos Brighente André Antonio Cutolo Gabriela Motoie Cristina da Silva Meira Vera Lúcia Pereira‐Chioccola

Phlebotomus

Kinetoplastida

10.1016/j.actatropica.2018.01.012

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Citations (9)

[Acute abdomen and malaria].

PubMed (1947)

Cristina da Silva Meira

Acute abdomen

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Analysis of the humoral response in Balb/c mice immunized with 255 Gy irradiated T. gondii tachyzoites and challenged with cysts of ME49 strain

Thais A. Costa‐Silva Andrés Jimenez Galisteo Cristina da Silva Meira Choccola Pereira Heitor Franco de Andrade

Strain (injury)

BALB/c

Humoral immunity

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Antígenos excretados/secretados de Toxoplasma gondii : resposta imune celular e humoral na toxoplasmose cerebral e Aids

Cristina da Silva Meira

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Human extracellular vesicles and correlation with two clinical forms of toxoplasmosis

PLoS ONE (2020)

Allecinéia Bispo da Cruz Marta Marques Maia Ingrid de Siqueira Pereira Noemi Nosomi Taniwaki Gislene Mitsue Namiyama

Aim This study analyzed microvesicles and exosomes, called as extracellular vesicles (EVs) excreted in serum and cerebrospinal fluid (CSF) from patients with cerebral or gestational toxoplasmosis. Methods Clinical samples from 83 individuals were divided into four groups. Group I, 20 sera from healthy individuals and pregnant women (seronegative for toxoplasmosis); group II, 21 sera from seropositive patients for toxoplasmosis (cerebral or gestational forms); group III, 26 CSF samples from patients with cerebral toxoplasmosis/HIV co-infection (CT/HIV) (seropositive for toxoplasmosis); and group IV, 16 CSF samples from seronegative patients for toxoplasmosis, but with HIV infection and other opportunistic infections (OI/HIV). Serum and CSF samples were ultracentrifuged to recover EVs. Next, vesicle size and concentration were characterized by Nanoparticle Tracking Analysis (NTA). Results Concentrations of serum-derived EVs from toxoplasmosis patients (mean: 2.4 x 1010 EVs/mL) were statically higher than of non-infected individuals (mean: 5.9 x 109 EVs/mL). Concentrations of CSF-derived EVs were almost similar in both groups. CT/HIV (mean: 2.9 x 109 EVs/mL) and OI/HIV (mean: 4.8 x 109 EVs/mL). Analyses by NTA confirmed that CSF-derived EVs and serum-derived EVs had size and shape similar to microvesicles and exosomes. The mean size of EVs was similar in serum and CSF. Thus, the concentration, and not size was able distinguish patients with toxoplasmosis than healthy individuals. Presence of exosomes was also confirmed by transmission electron microscopy and evidence of tetraspanins CD63 and CD9 in immunoblotting. Relative expressions of miR-146a-5p, miR-155-5p, miR-21-5p, miR-29c-3p and miR-125b-5p were estimated in exosomal miRNA extracted of EVs. Serum-derived EVs from group II (cerebral and gestational toxoplasmosis) up-expressed miR-125b-5p and miR-146a-5p. CSF-derived EVs from CT/HIV patients) up-expressed miR-155-5p and miR-21-5p and were unable to express miR-29c-3p. Conclusion These data suggest the participation of EVs and exosomal miRNAs in unbalance of immune response as elevation of TNF-α, IL-6; and downregulation of IFN-γ in cerebral and gestational forms of toxoplasmosis.

Toxoplasmosis

CD63

Extracellular Vesicles

Nanoparticle tracking analysis

10.1371/journal.pone.0229602

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Citations (27)