To investigate the clinical efficacy and influence on thyroid function of ultrasound-guided microwave ablation (UGMWA) in patients with nodular goiter.
Non-small cell lung cancer (NSCLC) accounts for more than 80% of lung cancer. CircRNA is a new type of non-coding RNA. CircRNA was found to be deeply involved in the regulation of NSCLC cells. However, the principle of CircRNA regulating NSCLC cells needs to be further explored.The relative mRNA expression levels of CircRNA_103993, miR-1271 and ERG were detected by quantificational real-time polymerase chain reaction (qRT-PCR) in NSCLC cells and human bronchial epithelial cell line HBE. Cell proliferation was detected by the Cell Counting Kit (CCK8) when NSCLC cells were transfected with si-CircRNA_103993, si-NC, miR-1271 mimics, miR-NC, LV-ERG, respectively. The apoptotic rate of NSCLC cells was measured by apoptotic assay and flow Cytometry. The relative mRNA and protein expression levels of PCNA and caspase-3 were detected by Western blot and qRT-PCR.CircRNA_103993 and ERG were significantly up-regulated while miR-1271 was significantly down-regulated in NSCLC cells. Knockdown of CircRNA_103993 and high expression of miR-1271 significantly inhibited NSCLC cell proliferation and promoted apoptosis. The double luciferin report showed that CircRNA_103993 served as a sponge of miR-1271 and miR-1271 could directly target ERG in NSCLC cells. More importantly, low expression of CircRNA_103993 increased the expression level of miR-1271, and high expression of miR-1271 decreased the expression level of ERG. Further experiments showed that miR-1271 inhibitors reversed the effect of si-CircRNA_103993 on proliferation and apoptosis of NSCLC cells. However, miR-1271 mimics significantly promoted the effects of si-CircRNA_103993 on the proliferation and apoptosis. Moreover, LV-ERG reversed the effect of miR-1271 mimics on proliferation and apoptosis of NSCLC cells. However, si-ERG significantly promoted the effects of miR-1271 mimics on the proliferation and apoptosis.CircRNA_103993 was highly expressed in NSCLC cells. CircRNA_103993 regulated proliferation and apoptosis of NSCLC cells by acting as a sponge of miR-1271. The CircRNA_103993 /miR-1271/ ERG axis had an important effect on the proliferation and apoptosis of NSCLC cells. Therefore, CircRNA_103993 may be a target for treating lung cancer.
<div>Abstract<p>Surgical resection followed by radiotherapy (RT) is recommended for malignant meningioma, but poor outcome is unavoidable. To improve the efficacy of RT in malignant meningioma, a targeted radiosensitizer can be added. Nicotinamide phosphoribosyltransferase (NAMPT), highly expressed in high-grade meningiomas, may play a role in determining the radioresponse. Herein, we evaluated the impact of NAMPT inhibition on radiosensitivity in malignant meningioma <i>in vivo</i> and <i>in vitro</i>. IOMM-Lee and TTMM705 cells were treated with NAMPT inhibition (FK866 or shRNA <i>NAMPT</i>) before irradiation. The subsequent clonogenic assay demonstrated significantly increased radiosensitivity. Combination treatment with FK866 and irradiation significantly increased the number of G2/M-phase cells, percentage of apoptotic cells, and γ-H2A.X level compared with FK866 or RT alone. We examined the effect of NAMPT inhibition on NMI and p53 expression in IOMM-Lee and TTMM705 cells. NAMPT inhibition by FK866 and shRNA treatment increased NMI, p53, CDKN1A and BAX expression. Additionally, we assessed the efficacy of FK866/RT combination treatment <i>in vivo</i>. The combination treatment exhibited increased antitumor efficacy compared with either treatment alone. The Ki67 level was significantly lower, and the p53 and γ-H2A.X levels were significantly higher in the combination treatment group than in the other three groups. In conclusion, these results indicate that FK866 improves radiosensitivity in malignant meningioma, an effect that may be attributed to the increase in p53 expression.</p></div>
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