Elaine Bélanger

Agriculture and Agri-Food Canada

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Guo-Ping Yang

Laboratoire des Interactions Plantes Micro-Organismes

Frédéric Debellé

Epicura

Jean Dénarié

Interactions Arbres-Microorganismes

Véréna Poinsot

Université Toulouse III - Paul Sabatier

Michel Treilhou

Université de Toulouse

Jean‐Claude Promé

Centre National de la Recherche Scientifique

Serge Laberge

Agriculture and Agri-Food Canada

Jean Cloutier

Agriculture and Agri-Food Canada

Hani Antoun

Université Laval

Cooperative Institutions

Centre National de la Recherche Scientifique

Laboratoire des Interactions Plantes Micro-Organismes

Université de Toulouse

Université Toulouse III - Paul Sabatier

Softmat - Chimie des colloïdes, polymères & assemblages complexes

Agriculture and Agri-Food Canada

Université Laval

Laboratoire de Microbiologie et Génétique Moléculaires

Epicura

University of Mons

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Unusual Methyl-Branched α,β-Unsaturated Acyl Chain Substitutions in the Nod Factors of an Arctic Rhizobium, Mesorhizobium sp. Strain N33 ( Oxytropis arctobia )

Journal of Bacteriology (2001)

Véréna Poinsot Elaine Bélanger Serge Laberge Guo-Ping Yang Hani Antoun

Mesorhizobium sp. strain N33 (Oxytropis arctobia), a rhizobial strain isolated in arctic Canada, is able to fix nitrogen at very low temperatures in association with a few arctic legume species belonging to the genera Astragalus, Onobrychis, and Oxytropis. Using mass spectrometry and nuclear magnetic resonance spectroscopy, we have determined the structure of N33 Nod factors, which are major determinants of nodulation. They are pentameric lipochito-oligosaccharides 6-O sulfated at the reducing end and exhibit other original substitutions: 6-O acetylation of the glucosamine residue next to the nonreducing terminal glucosamine and N acylation of the nonreducing terminal glucosamine by methyl-branched acyl chains of the iso series, some of which are alpha,beta unsaturated. These unusual substitutions may contribute to the peculiar host range of N33. Analysis of N33 whole-cell fatty acids indicated that synthesis of the methyl-branched fatty acids depended on the induction of bacteria by plant flavonoids, suggesting a specific role for these fatty acids in the signaling process between the plant and the bacteria. Synthesis of the methyl-branched alpha,beta-unsaturated fatty acids required a functional nodE gene.

Mesorhizobium

Nod factor

Strain (injury)

Sinorhizobium meliloti

10.1128/jb.183.12.3721-3728.2001

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